JP2014516512A5 - - Google Patents

Download PDF

Info

Publication number
JP2014516512A5
JP2014516512A5 JP2014504020A JP2014504020A JP2014516512A5 JP 2014516512 A5 JP2014516512 A5 JP 2014516512A5 JP 2014504020 A JP2014504020 A JP 2014504020A JP 2014504020 A JP2014504020 A JP 2014504020A JP 2014516512 A5 JP2014516512 A5 JP 2014516512A5
Authority
JP
Japan
Prior art keywords
particles
peg
subset
recombinase
fluorescence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2014504020A
Other languages
English (en)
Japanese (ja)
Other versions
JP2014516512A (ja
JP5961247B2 (ja
Filing date
Publication date
Application filed filed Critical
Priority claimed from PCT/US2012/032508 external-priority patent/WO2012138989A1/en
Publication of JP2014516512A publication Critical patent/JP2014516512A/ja
Publication of JP2014516512A5 publication Critical patent/JP2014516512A5/ja
Application granted granted Critical
Publication of JP5961247B2 publication Critical patent/JP5961247B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

JP2014504020A 2011-04-07 2012-04-06 リコンビナーゼポリメラーゼ増幅混合物のモニタリング Active JP5961247B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161472919P 2011-04-07 2011-04-07
US61/472,919 2011-04-07
PCT/US2012/032508 WO2012138989A1 (en) 2011-04-07 2012-04-06 Monitoring recombinase polymerase amplification mixtures

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2016067727A Division JP6209638B2 (ja) 2011-04-07 2016-03-30 リコンビナーゼポリメラーゼ増幅混合物のモニタリング

Publications (3)

Publication Number Publication Date
JP2014516512A JP2014516512A (ja) 2014-07-17
JP2014516512A5 true JP2014516512A5 (https=) 2015-05-28
JP5961247B2 JP5961247B2 (ja) 2016-08-02

Family

ID=46966393

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2014504020A Active JP5961247B2 (ja) 2011-04-07 2012-04-06 リコンビナーゼポリメラーゼ増幅混合物のモニタリング
JP2016067727A Active JP6209638B2 (ja) 2011-04-07 2016-03-30 リコンビナーゼポリメラーゼ増幅混合物のモニタリング

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2016067727A Active JP6209638B2 (ja) 2011-04-07 2016-03-30 リコンビナーゼポリメラーゼ増幅混合物のモニタリング

Country Status (10)

Country Link
US (3) US8809021B2 (https=)
EP (3) EP2694666B1 (https=)
JP (2) JP5961247B2 (https=)
CN (2) CN103476940B (https=)
AU (1) AU2012239989B2 (https=)
BR (1) BR112013025758B1 (https=)
CA (1) CA2831140C (https=)
ES (1) ES2905686T3 (https=)
WO (1) WO2012138989A1 (https=)
ZA (1) ZA201307184B (https=)

Families Citing this family (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
US8030000B2 (en) 2002-02-21 2011-10-04 Alere San Diego, Inc. Recombinase polymerase amplification
US8071308B2 (en) 2006-05-04 2011-12-06 Alere San Diego, Inc. Recombinase polymerase amplification
US9334531B2 (en) 2010-12-17 2016-05-10 Life Technologies Corporation Nucleic acid amplification
US9309566B2 (en) 2010-12-17 2016-04-12 Life Technologies Corporation Methods, compositions, systems, apparatuses and kits for nucleic acid amplification
US9309557B2 (en) 2010-12-17 2016-04-12 Life Technologies Corporation Nucleic acid amplification
US20120156728A1 (en) 2010-12-17 2012-06-21 Life Technologies Corporation Clonal amplification of nucleic acid on solid surface with template walking
US9399217B2 (en) 2010-10-04 2016-07-26 Genapsys, Inc. Chamber free nanoreactor system
US9184099B2 (en) 2010-10-04 2015-11-10 The Board Of Trustees Of The Leland Stanford Junior University Biosensor devices, systems and methods therefor
NZ610129A (en) 2010-10-04 2014-08-29 Genapsys Inc Systems and methods for automated reusable parallel biological reactions
US8585973B2 (en) 2011-05-27 2013-11-19 The Board Of Trustees Of The Leland Stanford Junior University Nano-sensor array
US9926596B2 (en) 2011-05-27 2018-03-27 Genapsys, Inc. Systems and methods for genetic and biological analysis
CN106591103B (zh) 2011-12-01 2021-06-04 吉纳普赛斯股份有限公司 用于高效电子测序与检测的系统和方法
EP2971141B1 (en) 2013-03-15 2018-11-28 Genapsys, Inc. Systems for biological analysis
JP6050555B2 (ja) * 2013-07-15 2016-12-21 シージーン アイエヌシー Ｐｔｏの切断及び延長−依存的固定化オリゴヌクレオチドハイブリダイゼーションを用いたターゲット核酸配列の検出
US11008625B2 (en) * 2013-11-22 2021-05-18 Aidian Oy Detection of nucleic acids by strand invasion based amplification
WO2015089238A1 (en) 2013-12-11 2015-06-18 Genapsys, Inc. Systems and methods for biological analysis and computation
EP3556864B1 (en) 2014-04-18 2020-12-09 Genapsys, Inc. Methods and systems for nucleic acid amplification
ES2828959T3 (es) * 2014-08-22 2021-05-28 Cepheid Métodos para detectar la influenza
KR102126641B1 (ko) * 2014-09-29 2020-06-24 일루미나 케임브리지 리미티드 재조합효소 돌연변이체
CN104830820A (zh) * 2015-05-07 2015-08-12 浙江泰晶生物科技有限公司 用于常温等温快速检测核糖核酸的蛋白酶及检测方法
EP3307908B1 (en) 2015-06-09 2019-09-11 Life Technologies Corporation Methods for molecular tagging
US20180291413A1 (en) 2015-10-06 2018-10-11 Thermo Fisher Scientific Geneart Gmbh Devices and methods for producing nucleic acids and proteins
GB201519565D0 (en) 2015-11-05 2015-12-23 Alere San Diego Inc Sample preparation device
US11118206B2 (en) 2016-01-13 2021-09-14 The Trustees Of The University Of Pennsylvania Multiple stage isothermal enzymatic amplification
WO2017147486A1 (en) * 2016-02-26 2017-08-31 Alere San Diego Inc. Redox labeled oligonucleotide probes and their use
CA3016582A1 (en) * 2016-03-04 2017-09-08 Alere San Diego, Inc. Automated nested recombinase polymerase amplification
AU2017241670B2 (en) 2016-03-28 2020-10-08 Illumina, Inc. Recombinase mutants
US9617587B1 (en) 2016-04-04 2017-04-11 Nat Diagnostics, Inc. Isothermal amplification components and processes
US11299777B2 (en) 2016-04-04 2022-04-12 Nat Diagnostics, Inc. Isothermal amplification components and processes
US10544456B2 (en) 2016-07-20 2020-01-28 Genapsys, Inc. Systems and methods for nucleic acid sequencing
EP3650543B1 (en) * 2017-07-05 2023-06-07 Oriciro Genomics, Inc. Dna production method and dna fragment joining kit
CA3076378A1 (en) 2017-09-21 2019-03-28 Genapsys, Inc. Systems and methods for nucleic acid sequencing
CN108195801B (zh) * 2017-11-17 2020-11-24 北京林业大学 单分子水平观测气孔保卫细胞膜蛋白分布和动态的方法
CN107893103A (zh) * 2017-11-29 2018-04-10 默禾医疗科技（上海）有限公司 重组酶聚合酶扩增中重组酶和蛋白浓度比及活性定量法
AU2019287163B2 (en) 2018-06-12 2025-08-21 Keygene N.V. Nucleic acid amplification method
GB201905303D0 (en) 2019-04-15 2019-05-29 Thermo Fisher Scient Geneart Gmbh Multiplex assembly of nucleic acid molecules
CN111154739B (zh) * 2020-02-06 2021-03-02 广州普世利华科技有限公司 一种新型重组酶依赖型扩增方法及试剂盒
US20230313323A1 (en) * 2020-03-26 2023-10-05 Ionian Technologies, Llc Assays for detecting coronavirus disease 2019 (covid-19)
US20210381067A1 (en) * 2020-06-08 2021-12-09 Ampliwise Inc. Nucleic acid amplification and detection with attenuaiting probe
EP4053293A1 (en) * 2021-03-03 2022-09-07 midge medical GmbH Protected isothermal nucleic acid amplification (pina) methods for point-of-need diagnosis of emerging infectious diseases
CA3218077A1 (en) 2021-04-29 2022-11-03 Abbott Laboratories Systems and methods for pooling samples for high-throughput analysis
CN114085891A (zh) * 2021-11-23 2022-02-25 广州达安基因股份有限公司 基于重组酶聚合酶扩增技术的逆转录扩增系统和方法

Family Cites Families (55)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US5418149A (en) 1990-07-24 1995-05-23 Hoffmann-La Roche Inc. Reduction of non-specific amplification glycosylase using DUTP and DNA uracil
US5858652A (en) 1988-08-30 1999-01-12 Abbott Laboratories Detection and amplification of target nucleic acid sequences
GB8903627D0 (en) 1989-02-17 1989-04-05 Unilever Plc Assays
CA2035010C (en) 1990-01-26 1996-12-10 Keith C. Backman Method of amplifying target nucleic acids applicable to both polymerase and ligase chain reactions
US5326692B1 (en) 1992-05-13 1996-04-30 Molecular Probes Inc Fluorescent microparticles with controllable enhanced stokes shift
US5273881A (en) 1990-05-07 1993-12-28 Daikin Industries, Ltd. Diagnostic applications of double D-loop formation
ATE153707T1 (de) 1990-05-07 1997-06-15 Daikin Ind Ltd Diagnostische anwendungen der bildung von doppelten d-loops.
US5223414A (en) 1990-05-07 1993-06-29 Sri International Process for nucleic acid hybridization and amplification
EP0543484B1 (en) 1991-08-30 2001-01-31 Research Development Corporation of Japan A method of DNA amplification
CA2122203C (en) 1993-05-11 2001-12-18 Melinda S. Fraiser Decontamination of nucleic acid amplification reactions
FR2708288B1 (fr) 1993-07-26 1995-09-01 Bio Merieux Procédé d'amplification d'acides nucléiques par transcription utilisant le déplacement, réactifs et nécessaire pour la mise en Óoeuvre de ce procédé.
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5705366A (en) 1994-09-15 1998-01-06 Johnson & Johnson Clinical Diagnostics, Inc. Coamplification of target nucleic acids using volume exclusion agent in reaction composition, test kit and test device useful therefor
US5656430A (en) 1995-06-07 1997-08-12 Trevigen, Inc. Oscillating signal amplifier for nucleic acid detection
US5853990A (en) 1996-07-26 1998-12-29 Edward E. Winger Real time homogeneous nucleotide assay
CA2263815A1 (en) 1996-08-29 1998-03-05 Koji Kigawa Methods for targeting, enriching, detecting and/or isolating target nucleic acid sequence using reca-like recombinase
JP3065035B2 (ja) * 1998-10-01 2000-07-12 ダイキン工業株式会社 高性能なＲｅｃＡ様組換え酵素／１本鎖核酸プローブ複合体の調製方法及びその利用
US6087112A (en) 1998-12-30 2000-07-11 Oligos Etc. Inc. Arrays with modified oligonucleotide and polynucleotide compositions
WO2000041524A2 (en) 1999-01-11 2000-07-20 President And Fellows Of Harvard College Isothermal amplification of dna
US6699693B1 (en) 1999-02-04 2004-03-02 Sloan-Kettering Institute For Cancer Research Process for DNA replication
US6387621B1 (en) 1999-04-27 2002-05-14 University Of Utah Research Foundation Automated analysis of real-time nucleic acid amplification
NO314091B1 (no) 2000-01-12 2003-01-27 Biotec Pharmacon Asa Varmelabil uracil-DNA-glykosylase, DNA-sekvens som koder for enzymet, mikroorganisme som inneholder DNA-sekvensen, samt anvendelse av enzymet
AU2001243670A1 (en) 2000-03-20 2001-10-03 Maxygen, Inc. Method for generating recombinant dna molecules in complex mixtures
US20020061530A1 (en) 2000-07-31 2002-05-23 Belotserkovskii Boris P. Enhanced targeting of DNA sequences by recombinase protein and single-stranded homologous DNA probes using DNA analog activation
US6379899B1 (en) 2001-03-13 2002-04-30 Discoverx Isothermal exponential RNA amplification in complex mixtures
CA2444649C (en) 2001-04-20 2012-10-02 The Penn State Research Foundation Methods for nucleic acid manipulation
EA006066B1 (ru) 2001-08-20 2005-08-25 Такара Био Инк. Способы амплификации нуклеиновых кислот
AU2002341898A2 (en) 2001-09-28 2003-04-07 University Of Delaware Polymorphism detection and separation
US7244562B2 (en) 2001-11-01 2007-07-17 Gene Check, Inc. RecA assisted detection of mutations, single nucleotide polymorphisms and specific sequences
US8030000B2 (en) * 2002-02-21 2011-10-04 Alere San Diego, Inc. Recombinase polymerase amplification
US7270981B2 (en) 2002-02-21 2007-09-18 Asm Scientific, Inc. Recombinase polymerase amplification
US7399590B2 (en) * 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
US20040137456A1 (en) 2002-04-04 2004-07-15 Hiroki Yokota Method for identifying and characterizing individual dna molecules
US20030228611A1 (en) 2002-05-01 2003-12-11 President And Fellows Of Harvard College Nucleic acid memory device
US20060160078A1 (en) 2002-07-12 2006-07-20 Cardy Donald L N Lateral flow assay device and method
EP1543154A4 (en) 2002-08-21 2006-08-16 Epoch Biosciences Inc ASSAY WITH ENDONUCLEASE FOR ABASIC SPOTS
CA2498764C (en) 2002-09-20 2015-11-10 New England Biolabs, Inc. Helicase dependent amplification of nucleic acids
EP1613768A4 (en) 2003-03-11 2006-05-31 Gene Check Inc RECA-SUPPORTED ALLELSPECIFIC OLIGONUCLEOTIDE EXTENSION PROCESS
WO2004104213A2 (en) 2003-05-15 2004-12-02 The Rockefeller University Nucleic acids and polypeptides of c1 bacteriophage and uses thereof
JP2005110621A (ja) 2003-10-10 2005-04-28 Aisin Seiki Co Ltd 核酸増幅方法及び核酸増幅用試薬キット
CA2497324A1 (en) 2004-02-17 2005-08-17 Affymetrix, Inc. Methods for fragmenting and labelling dna
WO2005103670A1 (ja) 2004-04-21 2005-11-03 Toray Industries, Inc. ラボオンチップ用基板
DE102005052752A1 (de) 2005-11-04 2007-05-10 Clondiag Chip Technologies Gmbh Vorrichtung und Verfahren zum Nachweis von molekularen Wechselwirkungen
EP3985125A1 (en) * 2004-06-01 2022-04-20 Abbott Diagnostics Scarborough, Inc. Recombinase polymerase amplification
US7745125B2 (en) 2004-06-28 2010-06-29 Roche Molecular Systems, Inc. 2′-terminator related pyrophosphorolysis activated polymerization
JP2008520245A (ja) 2004-11-23 2008-06-19 ワン，シャオ・ビン 切断−増幅法による核酸多様性の検出
WO2007096702A2 (en) 2005-07-25 2007-08-30 Asm Scientific, Inc. Methods for multiplexing recombinase polymerase amplification
US8071308B2 (en) * 2006-05-04 2011-12-06 Alere San Diego, Inc. Recombinase polymerase amplification
CN101743063B (zh) 2007-05-03 2012-12-12 科隆迪亚戈有限公司 分析方法
US8560130B2 (en) 2007-12-04 2013-10-15 Lester F. Ludwig Software controlled lab-on-a-chip emulation
US20090215050A1 (en) * 2008-02-22 2009-08-27 Robert Delmar Jenison Systems and methods for point-of-care amplification and detection of polynucleotides
CN108088824B (zh) 2008-03-14 2021-06-04 雅培快速诊断耶拿有限公司 一种用于检测分析物的系统
EP2438196B1 (en) 2009-06-05 2016-12-21 Alere San Diego, Inc. Recombinase polymerase amplification reagents and kits

Similar Documents

Publication Publication Date Title
JP2014516512A5 (https=)
CN105164279B (zh) 靶核酸的多重分析
US10982257B2 (en) Methods to profile molecular complexes or single cells via proximity dependent barcoding
US20210380974A1 (en) Combinatorial sets of nucleic acid barcodes for analysis of nucleic acids associated with single cells
US8945844B2 (en) Method of amplifying target nucleic acid with reduced amplification bias and method for determining relative amount of target nucleic acid in sample
KR102531677B1 (ko) 개별 세포 또는 세포 개체군으로부터 핵산을 분석하는 방법
JP2014512826A5 (https=)
JP2015519911A5 (https=)
JP2017510244A5 (https=)
JP2015156872A5 (https=)
US20180100192A1 (en) Surface concatemerization of templates
CN107012202A (zh) 核酸扩增的精度管理方法、精度管理用试剂及其试剂盒
US20230212561A1 (en) Accurate sequencing library generation via ultra-high partitioning
AU2014265791A1 (en) Analyte enrichment methods and compositions
CN107429292A (zh) 底数大于2的指数核酸扩增
CN102676637B (zh) 一种高专一性的测定小rna的方法
EP2761032A1 (en) Hydrolysis probes
EP3099819A2 (en) Isothermal methods and related compositions for preparing nucleic acids
WO2023133094A1 (en) Accurate sequencing library generation via ultra-high partitioning
JP2018518968A5 (https=)
CN108504706A (zh) 核酸扩增方法
JP2014083052A5 (https=)
CA2983428A1 (en) Kit for together detecting multiple target nucleic acids differing from each other and detection method using the same
Dupinay et al. Next-generation sequencing of ultra-low copy samples: from clinical FFPE samples to single-cell sequencing
JP2018014924A (ja) ヘリカーゼを用いたｐｃｒ