JP2013531978A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2013531978A5 JP2013531978A5 JP2013512251A JP2013512251A JP2013531978A5 JP 2013531978 A5 JP2013531978 A5 JP 2013531978A5 JP 2013512251 A JP2013512251 A JP 2013512251A JP 2013512251 A JP2013512251 A JP 2013512251A JP 2013531978 A5 JP2013531978 A5 JP 2013531978A5
- Authority
- JP
- Japan
- Prior art keywords
- pdhk
- nucleic acid
- acid sequence
- cell
- heterologous nucleic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US34972710P | 2010-05-28 | 2010-05-28 | |
| US61/349,727 | 2010-05-28 | ||
| PCT/US2011/038191 WO2011150241A2 (en) | 2010-05-28 | 2011-05-26 | Decreasing lactate level and increasing polypeptide production by downregulating the expression of lactate dehydrogenase and pyruvate dehydrogenase kinase |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016227876A Division JP6325060B2 (ja) | 2010-05-28 | 2016-11-24 | Ｌｄｈ及びｐｄｈｋ発現を下方制御することによる乳酸レベルの低下及びポリペプチド生産の増加 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2013531978A JP2013531978A (ja) | 2013-08-15 |
| JP2013531978A5 true JP2013531978A5 (https=) | 2014-07-10 |
| JP6050226B2 JP6050226B2 (ja) | 2016-12-21 |
Family
ID=45004831
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2013512251A Active JP6050226B2 (ja) | 2010-05-28 | 2011-05-26 | Ｌｄｈ及びｐｄｈｋ発現を下方制御することによる乳酸レベルの低下及びポリペプチド生産の増加 |
| JP2016227876A Active JP6325060B2 (ja) | 2010-05-28 | 2016-11-24 | Ｌｄｈ及びｐｄｈｋ発現を下方制御することによる乳酸レベルの低下及びポリペプチド生産の増加 |
| JP2018076234A Active JP6706642B2 (ja) | 2010-05-28 | 2018-04-11 | Ｌｄｈ及びｐｄｈｋ発現を下方制御することによる乳酸レベルの低下及びポリペプチド生産の増加 |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016227876A Active JP6325060B2 (ja) | 2010-05-28 | 2016-11-24 | Ｌｄｈ及びｐｄｈｋ発現を下方制御することによる乳酸レベルの低下及びポリペプチド生産の増加 |
| JP2018076234A Active JP6706642B2 (ja) | 2010-05-28 | 2018-04-11 | Ｌｄｈ及びｐｄｈｋ発現を下方制御することによる乳酸レベルの低下及びポリペプチド生産の増加 |
Country Status (15)
| Country | Link |
|---|---|
| US (4) | US9487809B2 (https=) |
| EP (1) | EP2576580B1 (https=) |
| JP (3) | JP6050226B2 (https=) |
| KR (3) | KR101869987B1 (https=) |
| CN (1) | CN102985437B (https=) |
| BR (1) | BR112012030197A2 (https=) |
| CA (1) | CA2800728C (https=) |
| DK (1) | DK2576580T3 (https=) |
| ES (1) | ES2599412T3 (https=) |
| HU (1) | HUE030820T2 (https=) |
| MX (3) | MX343604B (https=) |
| PL (1) | PL2576580T3 (https=) |
| RU (1) | RU2614125C2 (https=) |
| SI (1) | SI2576580T1 (https=) |
| WO (1) | WO2011150241A2 (https=) |
Families Citing this family (42)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2831860B2 (ja) | 1991-03-29 | 1998-12-02 | 株式会社東芝 | 連続ラインの連続式冷却槽温度制御装置 |
| TWI456062B (zh) | 2006-09-13 | 2014-10-11 | Abbvie Inc | 細胞培養改良 |
| US8911964B2 (en) | 2006-09-13 | 2014-12-16 | Abbvie Inc. | Fed-batch method of making human anti-TNF-alpha antibody |
| AU2009347206C1 (en) | 2008-10-20 | 2016-12-08 | Abbvie Inc. | Isolation and purification of antibodies using Protein A affinity chromatography |
| MX2011004201A (es) | 2008-10-20 | 2011-05-24 | Abbott Lab | Desactivacion viral durante purificacion de anticuerpos. |
| PL2576580T3 (pl) | 2010-05-28 | 2017-03-31 | F.Hoffmann-La Roche Ag | Zmniejszenie poziomu mleczanu i zwiększenie wytwarzania polipeptydu przez obniżenie poziomu ekspresji dehydrogenazy mleczanowej i kinazy dehydrogenazy pirogronianowej |
| EP2702077A2 (en) | 2011-04-27 | 2014-03-05 | AbbVie Inc. | Methods for controlling the galactosylation profile of recombinantly-expressed proteins |
| US9150645B2 (en) | 2012-04-20 | 2015-10-06 | Abbvie, Inc. | Cell culture methods to reduce acidic species |
| WO2013158273A1 (en) | 2012-04-20 | 2013-10-24 | Abbvie Inc. | Methods to modulate c-terminal lysine variant distribution |
| US9067990B2 (en) | 2013-03-14 | 2015-06-30 | Abbvie, Inc. | Protein purification using displacement chromatography |
| US9249182B2 (en) | 2012-05-24 | 2016-02-02 | Abbvie, Inc. | Purification of antibodies using hydrophobic interaction chromatography |
| HK1211981A1 (en) | 2012-09-02 | 2016-06-03 | Abbvie Inc. | Methods to control protein heterogeneity |
| US9512214B2 (en) | 2012-09-02 | 2016-12-06 | Abbvie, Inc. | Methods to control protein heterogeneity |
| SG11201507230PA (en) | 2013-03-12 | 2015-10-29 | Abbvie Inc | Human antibodies that bind human tnf-alpha and methods of preparing the same |
| WO2014151878A2 (en) | 2013-03-14 | 2014-09-25 | Abbvie Inc. | Methods for modulating protein glycosylation profiles of recombinant protein therapeutics using monosaccharides and oligosacharides |
| US8921526B2 (en) | 2013-03-14 | 2014-12-30 | Abbvie, Inc. | Mutated anti-TNFα antibodies and methods of their use |
| US9017687B1 (en) | 2013-10-18 | 2015-04-28 | Abbvie, Inc. | Low acidic species compositions and methods for producing and using the same using displacement chromatography |
| AR095196A1 (es) * | 2013-03-15 | 2015-09-30 | Regeneron Pharma | Medio de cultivo celular libre de suero |
| SMT202100691T1 (it) | 2013-07-11 | 2022-01-10 | Modernatx Inc | Composizioni 5 comprendenti polinucleotidi sintetici che codificano proteine correlate a crispr e sgrna sintetici e metodi d'uso |
| US9598667B2 (en) | 2013-10-04 | 2017-03-21 | Abbvie Inc. | Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins |
| US20160244725A1 (en) * | 2013-10-11 | 2016-08-25 | Regeneron Pharmaceuticals, Inc. | Metabolically optimized cell culture |
| US11390663B2 (en) | 2013-10-11 | 2022-07-19 | Regeneron Pharmaceuticals, Inc. | Metabolically optimized cell culture |
| US8946395B1 (en) | 2013-10-18 | 2015-02-03 | Abbvie Inc. | Purification of proteins using hydrophobic interaction chromatography |
| US9085618B2 (en) | 2013-10-18 | 2015-07-21 | Abbvie, Inc. | Low acidic species compositions and methods for producing and using the same |
| US9181337B2 (en) | 2013-10-18 | 2015-11-10 | Abbvie, Inc. | Modulated lysine variant species compositions and methods for producing and using the same |
| US20150139988A1 (en) | 2013-11-15 | 2015-05-21 | Abbvie, Inc. | Glycoengineered binding protein compositions |
| WO2015142903A2 (en) * | 2014-03-17 | 2015-09-24 | Genentech, Inc. | Method of controlling lactate production with piperdine-dione derivatives |
| AU2015330670B2 (en) * | 2014-10-10 | 2022-01-06 | Novo Nordisk Health Care Ag | Therapeutic inhibition of lactate dehydrogenase and agents therefor |
| KR102265924B1 (ko) | 2014-10-31 | 2021-06-15 | 삼성바이오에피스 주식회사 | Pdk 불활성화에 의한 폴리펩타이드의 생산 |
| WO2016125937A1 (ko) * | 2015-02-06 | 2016-08-11 | 경북대학교 산학협력단 | Pdk2를 이용한 여성 갱년기 질환 예방 또는 치료제의 스크리닝 방법 및 상기 방법으로 스크리닝 된 치료제를 유효성분으로 포함하는 여성 갱년기 질환의 예방 또는 치료용 약학적 조성물 |
| CN105368839A (zh) * | 2015-12-22 | 2016-03-02 | 中国人民解放军第三军医大学 | 一种有效抑制小鼠pdk2表达的rna干扰序列及其应用 |
| EP3452080A4 (en) * | 2016-05-02 | 2019-10-09 | The Regents of the University of California | MAMMALIAN CELLS WITHOUT LACTATE DEHYDROGENASE ACTIVITY |
| HRP20231063T1 (hr) | 2017-10-13 | 2023-12-22 | Novo Nordisk Health Care Ag | Postupci i pripravci namijenjeni inhibiranju eksprimiranja ldha |
| CN107904257B (zh) * | 2017-11-22 | 2021-05-04 | 上海药明生物技术有限公司 | 一种增加cho细胞群蛋白表达量的方法 |
| JP2021505662A (ja) * | 2017-12-04 | 2021-02-18 | ザ ジェネラル ホスピタル コーポレイション | 疾患を軽減するための細胞外酸化還元酵素系 |
| HRP20260157T1 (hr) * | 2018-03-29 | 2026-03-27 | F. Hoffmann - La Roche Ag | Moduliranje laktogene aktivnosti u stanicama sisavaca |
| MX2021015823A (es) * | 2019-06-26 | 2022-02-03 | Hoffmann La Roche | Lineas celulares de mamifero con inactivacion del gen sirtuina-1 (sirt-1). |
| US12297451B1 (en) | 2019-10-25 | 2025-05-13 | Regeneron Pharmaceuticals, Inc. | Cell culture medium |
| US12188060B2 (en) | 2020-05-15 | 2025-01-07 | Crispr Therapeutics Ag | Messenger RNA encoding Cas9 for use in genome-editing systems |
| CN115032286B (zh) * | 2021-03-08 | 2024-08-13 | 江成鸿 | 组合物在制备诊断婴幼儿血管瘤和/或监测其进展及预后的药物中的应用及试剂盒和药物 |
| CN115948404B (zh) * | 2023-02-01 | 2024-06-04 | 重庆医科大学 | 一种抑制环状RNA circPDHK1的siRNA及其用途 |
| EP4574966A1 (en) * | 2023-12-18 | 2025-06-25 | Good Meat, Inc. | Engineered cell lines for the production of cultivated meat |
Family Cites Families (58)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US30985A (en) | 1860-12-18 | Thomas l | ||
| USRE30985E (en) | 1978-01-01 | 1982-06-29 | Serum-free cell culture media | |
| FR2413974A1 (fr) | 1978-01-06 | 1979-08-03 | David Bernard | Sechoir pour feuilles imprimees par serigraphie |
| US4515893A (en) | 1979-04-26 | 1985-05-07 | Ortho Pharmaceutical Corporation | Hybrid cell line for producing complement-fixing monoclonal antibody to human T cells |
| US4560655A (en) | 1982-12-16 | 1985-12-24 | Immunex Corporation | Serum-free cell culture medium and process for making same |
| US4657866A (en) | 1982-12-21 | 1987-04-14 | Sudhir Kumar | Serum-free, synthetic, completely chemically defined tissue culture media |
| US4816567A (en) | 1983-04-08 | 1989-03-28 | Genentech, Inc. | Recombinant immunoglobin preparations |
| DD266710A3 (de) | 1983-06-06 | 1989-04-12 | Ve Forschungszentrum Biotechnologie | Verfahren zur biotechnischen Herstellung van alkalischer Phosphatase |
| US4767704A (en) | 1983-10-07 | 1988-08-30 | Columbia University In The City Of New York | Protein-free culture medium |
| US5672347A (en) | 1984-07-05 | 1997-09-30 | Genentech, Inc. | Tumor necrosis factor antagonists and their use |
| US4879231A (en) | 1984-10-30 | 1989-11-07 | Phillips Petroleum Company | Transformation of yeasts of the genus pichia |
| GB8516415D0 (en) | 1985-06-28 | 1985-07-31 | Celltech Ltd | Culture of animal cells |
| US4676980A (en) | 1985-09-23 | 1987-06-30 | The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Target specific cross-linked heteroantibodies |
| US5091178A (en) | 1986-02-21 | 1992-02-25 | Oncogen | Tumor therapy with biologically active anti-tumor antibodies |
| US4927762A (en) | 1986-04-01 | 1990-05-22 | Cell Enterprises, Inc. | Cell culture medium with antioxidant |
| GB8610600D0 (en) | 1986-04-30 | 1986-06-04 | Novo Industri As | Transformation of trichoderma |
| IL85035A0 (en) | 1987-01-08 | 1988-06-30 | Int Genetic Eng | Polynucleotide molecule,a chimeric antibody with specificity for human b cell surface antigen,a process for the preparation and methods utilizing the same |
| DE3850542T2 (de) | 1987-09-23 | 1994-11-24 | Bristol Myers Squibb Co | Antikörper-Heterokonjugate zur Töting von HIV-infizierten Zellen. |
| US5091313A (en) | 1988-08-05 | 1992-02-25 | Tanox Biosystems, Inc. | Antigenic epitopes of IgE present on B cell but not basophil surface |
| US5720937A (en) | 1988-01-12 | 1998-02-24 | Genentech, Inc. | In vivo tumor detection assay |
| EP0420937B1 (en) | 1988-06-21 | 1994-11-09 | Genentech, Inc. | Therapeutic compositions for the treatment of myocardial infarction |
| AU632065B2 (en) | 1988-09-23 | 1992-12-17 | Novartis Vaccines And Diagnostics, Inc. | Cell culture medium for enhanced cell growth, culture longevity and product expression |
| US5530101A (en) | 1988-12-28 | 1996-06-25 | Protein Design Labs, Inc. | Humanized immunoglobulins |
| EP0402226A1 (en) | 1989-06-06 | 1990-12-12 | Institut National De La Recherche Agronomique | Transformation vectors for yeast yarrowia |
| ES2096590T3 (es) | 1989-06-29 | 1997-03-16 | Medarex Inc | Reactivos biespecificos para la terapia del sida. |
| US5122469A (en) | 1990-10-03 | 1992-06-16 | Genentech, Inc. | Method for culturing Chinese hamster ovary cells to improve production of recombinant proteins |
| EP0586505A1 (en) | 1991-05-14 | 1994-03-16 | Repligen Corporation | Heteroconjugate antibodies for treatment of hiv infection |
| CA2113813C (en) | 1991-08-14 | 2005-04-12 | Paula M. Jardieu | Immunoglobulin variants for specific fc epsilon receptors |
| WO1993008829A1 (en) | 1991-11-04 | 1993-05-13 | The Regents Of The University Of California | Compositions that mediate killing of hiv-infected cells |
| AU675929B2 (en) | 1992-02-06 | 1997-02-27 | Curis, Inc. | Biosynthetic binding protein for cancer marker |
| WO1994004188A1 (en) | 1992-08-21 | 1994-03-03 | Genentech, Inc. | Method for treating an lfa-1-mediated disorder |
| US5736137A (en) | 1992-11-13 | 1998-04-07 | Idec Pharmaceuticals Corporation | Therapeutic application of chimeric and radiolabeled antibodies to human B lymphocyte restricted differentiation antigen for treatment of B cell lymphoma |
| DK0733207T3 (da) | 1993-12-10 | 1998-04-20 | Genentech Inc | Fremgangsmåder til diagnose af allergi og screening af anti-allergi-terapeutika |
| DK0739214T3 (da) | 1994-01-18 | 1998-10-07 | Genentech Inc | Fremgangsmåde til behandling af parasitinfektionerunder anvendelse af IgE antagonister |
| US5702946A (en) | 1994-03-03 | 1997-12-30 | Genentech, Inc. | Anti-IL-8 monoclonal antibodies for treatment of inflammatory disorders |
| US5731168A (en) | 1995-03-01 | 1998-03-24 | Genentech, Inc. | Method for making heteromultimeric polypeptides |
| IL117645A (en) | 1995-03-30 | 2005-08-31 | Genentech Inc | Vascular endothelial cell growth factor antagonists for use as medicaments in the treatment of age-related macular degeneration |
| AU6267896A (en) | 1995-06-07 | 1996-12-30 | Imclone Systems Incorporated | Antibody and antibody fragments for inhibiting the growth oftumors |
| IL125255A (en) | 1996-01-23 | 2002-02-10 | Genentech Inc | Anti-cd18 antibodies for treating stroke |
| US5726856A (en) | 1996-02-28 | 1998-03-10 | General Electric Company | Hexasubstituted guanidinium salts and ultracapacitors employing them as electrolytes |
| US5714388A (en) | 1996-08-14 | 1998-02-03 | Bayer Corporation | Apparatus and method for detecting chemiluminescent light |
| US7147851B1 (en) | 1996-08-15 | 2006-12-12 | Millennium Pharmaceuticals, Inc. | Humanized immunoglobulin reactive with α4β7 integrin |
| WO1998023761A1 (en) | 1996-11-27 | 1998-06-04 | Genentech, Inc. | HUMANIZED ANTI-CD11a ANTIBODIES |
| JP3957765B2 (ja) | 1997-04-07 | 2007-08-15 | ジェネンテク・インコーポレイテッド | 抗ｖｅｇｆ抗体 |
| EP0981618B2 (en) | 1997-05-15 | 2011-08-24 | Genentech, Inc. | Anti-apo-2 antibody |
| US6946129B1 (en) | 1999-06-08 | 2005-09-20 | Seattle Genetics, Inc. | Recombinant anti-CD40 antibody and uses thereof |
| AU783250B2 (en) | 1999-06-17 | 2005-10-06 | Dartmouth College | Autologous adoptive immunotherapy with primed antigen-specific T cells or B cells |
| CA2376596C (en) | 1999-06-25 | 2009-10-06 | Genentech, Inc. | Humanized anti-erbb2 antibodies and treatment with anti-erbb2 antibodies |
| AU778199B2 (en) | 1999-10-29 | 2004-11-25 | Genentech Inc. | Anti-prostate stem cell antigen (PSCA) antibody compositions and methods of use |
| WO2002004598A2 (en) * | 2000-07-12 | 2002-01-17 | Immunex Corporation | Improvement of cell culture performance by inhibitors of pyruvate dehydrogenase kinase |
| EP2345720A3 (en) * | 2001-07-12 | 2012-01-25 | University of Massachusetts | In vivo production of small interfering RNAs that mediate gene silencing |
| WO2006085942A2 (en) | 2004-06-17 | 2006-08-17 | The Regents Of The University Of California | Compositions and methods for regulating gene transcription |
| US20090209618A1 (en) * | 2004-10-08 | 2009-08-20 | Dang Chi V | Pyruvate dehydrogenase kinases as therapeutic targets for cancer and ischemic diseases |
| DK2186905T3 (en) | 2007-08-07 | 2016-09-19 | Chugai Pharmaceutical Co Ltd | Method of Preparation of Heterogeneous Protein |
| UA106047C2 (uk) * | 2008-06-13 | 2014-07-25 | Сентокор Орто Байотек Інк. | Спосіб отримання високої щільності життєздатних клітин в культурі клітин ссавців |
| HRP20180738T1 (hr) * | 2008-09-15 | 2018-06-15 | F. Hoffmann - La Roche Ag | Pripravci i postupci za regulaciju stanične osmolarnosti |
| PL2576580T3 (pl) | 2010-05-28 | 2017-03-31 | F.Hoffmann-La Roche Ag | Zmniejszenie poziomu mleczanu i zwiększenie wytwarzania polipeptydu przez obniżenie poziomu ekspresji dehydrogenazy mleczanowej i kinazy dehydrogenazy pirogronianowej |
| WO2015198451A1 (ja) | 2014-06-26 | 2015-12-30 | 楽天株式会社 | 情報処理装置、情報処理方法及び情報処理プログラム |
-
2011
- 2011-05-26 PL PL11787435T patent/PL2576580T3/pl unknown
- 2011-05-26 BR BR112012030197A patent/BR112012030197A2/pt not_active Application Discontinuation
- 2011-05-26 JP JP2013512251A patent/JP6050226B2/ja active Active
- 2011-05-26 MX MX2012013579A patent/MX343604B/es active IP Right Grant
- 2011-05-26 KR KR1020127033948A patent/KR101869987B1/ko not_active Expired - Fee Related
- 2011-05-26 WO PCT/US2011/038191 patent/WO2011150241A2/en not_active Ceased
- 2011-05-26 SI SI201131017A patent/SI2576580T1/sl unknown
- 2011-05-26 EP EP11787435.4A patent/EP2576580B1/en active Active
- 2011-05-26 CN CN201180026517.2A patent/CN102985437B/zh active Active
- 2011-05-26 MX MX2016014767A patent/MX374851B/es unknown
- 2011-05-26 HU HUE11787435A patent/HUE030820T2/en unknown
- 2011-05-26 CA CA2800728A patent/CA2800728C/en active Active
- 2011-05-26 DK DK11787435.4T patent/DK2576580T3/en active
- 2011-05-26 KR KR1020187016873A patent/KR101989762B1/ko not_active Expired - Fee Related
- 2011-05-26 RU RU2012157307A patent/RU2614125C2/ru active
- 2011-05-26 ES ES11787435.4T patent/ES2599412T3/es active Active
- 2011-05-26 KR KR1020197016381A patent/KR20190067277A/ko not_active Withdrawn
-
2012
- 2012-11-22 MX MX2020009460A patent/MX2020009460A/es unknown
- 2012-11-28 US US13/688,075 patent/US9487809B2/en active Active
-
2016
- 2016-09-16 US US15/268,424 patent/US10011856B2/en active Active
- 2016-11-24 JP JP2016227876A patent/JP6325060B2/ja active Active
-
2018
- 2018-04-11 JP JP2018076234A patent/JP6706642B2/ja active Active
- 2018-05-31 US US15/994,950 patent/US10704071B2/en active Active
-
2020
- 2020-05-29 US US16/888,344 patent/US11046987B2/en active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2013531978A5 (https=) | ||
| RU2012157307A (ru) | Снижение уровня лактата и увеличение продукции полипептида путем ингибирования экспрессии лактатдегидрогеназы и киназы пируватдегидрогеназы | |
| JP2023182587A5 (https=) | ||
| JP2010110331A5 (https=) | ||
| JP2012503478A5 (https=) | ||
| MY169935A (en) | "a method for reducing heterogeneity of antibodies and a process of producing the antibodies thereof" | |
| JP2012065652A5 (https=) | ||
| MX352746B (es) | Aceites específicos producidos a partir de microorganismos heterótrofos recombinantes. | |
| BR112015019950A2 (pt) | Método para gerar linhagem de células-tronco embrionárias (es) de rato, e, cultura in vitro | |
| MY166973A (en) | Methods for reducing accumulation of lactate during culturing and method for producing polypeptide | |
| MX337128B (es) | Sistemas de celulas y metodos para mejorar la sintesis de acidos grasos mediante la expresion de deshidrogenasas. | |
| MY166543A (en) | A microorganism for methionine production with enhanced glucose import | |
| Liu et al. | Development of genetic tools in glucoamylase-hyperproducing industrial Aspergillus niger strains | |
| US20160177300A1 (en) | Means and methods for the generation of mammalian producer cells for the production of recombinant proteins | |
| CN104152402A (zh) | 一种半滑舌鳎伪雄鱼性腺细胞系的构建与应用方法 | |
| CN103146525A (zh) | 绵柔型复合多微功能曲生产方法 | |
| CN108841864A (zh) | 一种利用rna干扰机制的分子传感器 | |
| CN103255238A (zh) | 一种控制哺乳动物细胞乳酸生成的方法 | |
| CN103361352A (zh) | 鹅原代肝细胞scd1基因沉默试剂盒及其应用 | |
| CN103397034A (zh) | miRNA-19a作为哺乳动物宿主细胞增殖调控靶标的应用 | |
| CN116121301A (zh) | miRNA在CHO细胞重组蛋白表达中的应用 | |
| CN104232804B (zh) | 一种梯度变温诱导提高透明质酸酶表达量的方法 | |
| CN109517800B (zh) | 一株补强胆固醇内源性合成的重构st细胞及其构建方法和用途 | |
| Tian et al. | RNAi-based Boolean gates in the yeast Saccharomyces cerevisiae | |
| Falco et al. | Fed-batch production of endoglucanase with a recombinant industrial strain of the yeast Saccharomyces cerevisiae |