JP2013524826A5 - - Google Patents
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- Publication number
- JP2013524826A5 JP2013524826A5 JP2013506613A JP2013506613A JP2013524826A5 JP 2013524826 A5 JP2013524826 A5 JP 2013524826A5 JP 2013506613 A JP2013506613 A JP 2013506613A JP 2013506613 A JP2013506613 A JP 2013506613A JP 2013524826 A5 JP2013524826 A5 JP 2013524826A5
- Authority
- JP
- Japan
- Prior art keywords
- cell culture
- culture medium
- mmol
- amino acids
- recombinant polypeptide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims 8
- 239000006143 cell culture medium Substances 0.000 claims 7
- 229940024606 amino acid Drugs 0.000 claims 3
- 235000001014 amino acid Nutrition 0.000 claims 3
- 150000001413 amino acids Chemical class 0.000 claims 3
- 229920001184 polypeptide Polymers 0.000 claims 3
- 102000004196 processed proteins & peptides Human genes 0.000 claims 3
- 108090000765 processed proteins & peptides Proteins 0.000 claims 3
- 239000001763 2-hydroxyethyl(trimethyl)azanium Substances 0.000 claims 2
- 235000019743 Choline chloride Nutrition 0.000 claims 2
- 239000004381 Choline salt Substances 0.000 claims 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims 2
- 229960003178 choline chloride Drugs 0.000 claims 2
- SGMZJAMFUVOLNK-UHFFFAOYSA-M choline chloride Chemical compound [Cl-].C[N+](C)(C)CCO SGMZJAMFUVOLNK-UHFFFAOYSA-M 0.000 claims 2
- 235000019417 choline salt Nutrition 0.000 claims 2
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 claims 2
- 238000004519 manufacturing process Methods 0.000 claims 2
- 239000002609 medium Substances 0.000 claims 2
- 150000003248 quinolines Chemical class 0.000 claims 2
- 239000004475 Arginine Substances 0.000 claims 1
- DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 claims 1
- 239000004471 Glycine Substances 0.000 claims 1
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 claims 1
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 claims 1
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 claims 1
- LEVWYRKDKASIDU-IMJSIDKUSA-N L-cystine Chemical compound [O-]C(=O)[C@@H]([NH3+])CSSC[C@H]([NH3+])C([O-])=O LEVWYRKDKASIDU-IMJSIDKUSA-N 0.000 claims 1
- AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 claims 1
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 claims 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 claims 1
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 claims 1
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 claims 1
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 claims 1
- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 claims 1
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 claims 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 claims 1
- 239000004472 Lysine Substances 0.000 claims 1
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 claims 1
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 claims 1
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 claims 1
- 239000004473 Threonine Substances 0.000 claims 1
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 claims 1
- KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 claims 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 claims 1
- 229960001230 asparagine Drugs 0.000 claims 1
- 235000009582 asparagine Nutrition 0.000 claims 1
- 235000003704 aspartic acid Nutrition 0.000 claims 1
- 238000010923 batch production Methods 0.000 claims 1
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 claims 1
- 210000004027 cell Anatomy 0.000 claims 1
- 210000004978 chinese hamster ovary cell Anatomy 0.000 claims 1
- 229960003067 cystine Drugs 0.000 claims 1
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 claims 1
- AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 claims 1
- 229960000310 isoleucine Drugs 0.000 claims 1
- 229930182817 methionine Natural products 0.000 claims 1
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 claims 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 claims 1
- 239000004474 valine Substances 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US32783710P | 2010-04-26 | 2010-04-26 | |
| US61/327,837 | 2010-04-26 | ||
| PCT/EP2011/056509 WO2011134921A1 (en) | 2010-04-26 | 2011-04-25 | Improved cell culture medium |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015204453A Division JP2016052310A (ja) | 2010-04-26 | 2015-10-16 | 改良型細胞培養培地 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2013524826A JP2013524826A (ja) | 2013-06-20 |
| JP2013524826A5 true JP2013524826A5 (enExample) | 2013-11-28 |
| JP6113653B2 JP6113653B2 (ja) | 2017-04-12 |
Family
ID=44276356
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2013506613A Active JP6113653B2 (ja) | 2010-04-26 | 2011-04-25 | 改良型細胞培養培地 |
| JP2015204453A Pending JP2016052310A (ja) | 2010-04-26 | 2015-10-16 | 改良型細胞培養培地 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015204453A Pending JP2016052310A (ja) | 2010-04-26 | 2015-10-16 | 改良型細胞培養培地 |
Country Status (14)
| Country | Link |
|---|---|
| US (2) | US8986957B2 (enExample) |
| EP (2) | EP2563903B1 (enExample) |
| JP (2) | JP6113653B2 (enExample) |
| KR (1) | KR101848945B1 (enExample) |
| CN (2) | CN102858953B (enExample) |
| AU (1) | AU2011246504B2 (enExample) |
| BR (1) | BR112012027434B1 (enExample) |
| CA (1) | CA2797140C (enExample) |
| ES (1) | ES2755907T3 (enExample) |
| IL (1) | IL222453B (enExample) |
| MX (1) | MX2012012525A (enExample) |
| RU (1) | RU2563353C2 (enExample) |
| SG (1) | SG185037A1 (enExample) |
| WO (1) | WO2011134921A1 (enExample) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BR112012027434B1 (pt) | 2010-04-26 | 2020-09-29 | Novartis Ag | Processo para produção de um polipeptídeo recombinante compreendendo uma fase de produção |
| WO2014054744A1 (ja) * | 2012-10-03 | 2014-04-10 | 協和発酵キリン株式会社 | 培養液にアミノ酸を添加することによるポリペプチドの還元防止方法 |
| JP2015531244A (ja) | 2012-10-15 | 2015-11-02 | ブリストル−マイヤーズ スクイブ カンパニーＢｒｉｓｔｏｌ−Ｍｙｅｒｓ Ｓｑｕｉｂｂ Ｃｏｍｐａｎｙ | タンパク質を製造するための哺乳動物細胞培養方法 |
| CA2943667C (en) * | 2014-01-29 | 2022-11-08 | Lg Life Sciences Ltd. | Method for modulating galactosylation of recombinant protein through optimization of culture medium |
| WO2015155975A1 (ja) * | 2014-04-07 | 2015-10-15 | 東洋製罐グループホールディングス株式会社 | 動物細胞用培養液、及び培養容器 |
| ES2733298T3 (es) | 2014-07-18 | 2019-11-28 | Sandoz Ag | Cuantificación de TNFR2:Fc plegado erróneamente |
| KR20240093910A (ko) | 2015-04-01 | 2024-06-24 | 베링거 인겔하임 인터내셔날 게엠베하 | 세포 배양 배지 |
| WO2016192830A1 (en) * | 2015-05-29 | 2016-12-08 | Merck Patent Gmbh | Deep eutectic solvents and/or ionic liquids in cell culture media |
| DK3303554T3 (da) * | 2015-05-29 | 2020-08-31 | Merck Patent Gmbh | Dybe eutektiske opløsningsmidler og/eller ioniske væsker som fodringsmedie |
| WO2016207721A1 (en) | 2015-06-25 | 2016-12-29 | University Of Limerick | Mechanical device for generating combinatorial library |
| WO2017083224A1 (en) * | 2015-11-09 | 2017-05-18 | Bristol-Myers Squibb Company | Methods to manipulate quality attributes of polypeptides produced in cho cells |
| KR20190005966A (ko) * | 2016-05-10 | 2019-01-16 | 제넨테크, 인크. | 폴리펩타이드의 재조합 생산 동안 트리설파이드 결합을 감소시키기 위한 방법 |
| KR102893388B1 (ko) * | 2016-10-31 | 2025-11-28 | 삼성바이오에피스 주식회사 | 세포 배양 배지에서 원치 않는 배양 부산물을 감소시키는 공정 |
| CN106676058B (zh) * | 2016-12-09 | 2020-06-19 | 中农威特生物科技股份有限公司 | 一种bhk21悬浮细胞高密度流加培养方法及其在口蹄疫病毒增殖中的应用 |
| GB201708655D0 (en) * | 2017-05-31 | 2017-07-12 | Ucb Biopharma Sprl | Cell culture methods |
| KR20220097421A (ko) | 2019-11-05 | 2022-07-07 | 아지노모토 가부시키가이샤 | 단백질의 제조 방법 |
| CA3167733A1 (en) * | 2020-01-14 | 2021-07-22 | Ajinomoto Co., Inc. | High-density cell culture method |
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| US4767704A (en) * | 1983-10-07 | 1988-08-30 | Columbia University In The City Of New York | Protein-free culture medium |
| US6048728A (en) | 1988-09-23 | 2000-04-11 | Chiron Corporation | Cell culture medium for enhanced cell growth, culture longevity, and product expression |
| US5266479A (en) * | 1989-04-12 | 1993-11-30 | Board Of Regents, The University Of Texas System | High calcium chemically defined culture medium |
| US5122469A (en) | 1990-10-03 | 1992-06-16 | Genentech, Inc. | Method for culturing Chinese hamster ovary cells to improve production of recombinant proteins |
| WO1992015670A1 (en) * | 1991-02-28 | 1992-09-17 | Hyclone Laboratories, Inc. | Blended bovine sera cellular growth media and their methods of production and use |
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| JP2000507812A (ja) * | 1996-03-12 | 2000-06-27 | ライフ テクノロジーズ，インコーポレイテッド | 造血細胞培養栄養補充成分 |
| EP2243827B2 (en) | 1996-08-30 | 2017-11-22 | Life Technologies Corporation | Serum-free mammalian cell culture medium, and uses thereof |
| JP4543402B2 (ja) | 1996-10-10 | 2010-09-15 | ライフ テクノロジーズ コーポレーション | 植物由来栄養素を含む動物細胞培養培地 |
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| ES2695999T3 (es) | 2007-10-12 | 2019-01-11 | Hoffmann La Roche | Expresión de proteínas a partir de múltiples ácidos nucleicos |
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| CN101381702A (zh) * | 2008-09-10 | 2009-03-11 | 吉林省赛达生物科技有限公司 | 无动物组分细胞培养基 |
| EP2342318A1 (en) * | 2008-09-26 | 2011-07-13 | Schering Corporation | High titer antibody production |
| BR112012027434B1 (pt) | 2010-04-26 | 2020-09-29 | Novartis Ag | Processo para produção de um polipeptídeo recombinante compreendendo uma fase de produção |
| CA2795461C (en) | 2010-04-26 | 2018-04-24 | Novartis Ag | Improved cell cultivation process |
| PT2563904E (pt) | 2010-04-26 | 2015-05-12 | Novartis Ag | Meio de cultura de células aperfeiçoado |
-
2011
- 2011-04-25 BR BR112012027434-1A patent/BR112012027434B1/pt active IP Right Grant
- 2011-04-25 AU AU2011246504A patent/AU2011246504B2/en active Active
- 2011-04-25 EP EP11716890.6A patent/EP2563903B1/en active Active
- 2011-04-25 CA CA2797140A patent/CA2797140C/en active Active
- 2011-04-25 EP EP19192479.4A patent/EP3599276A1/en active Pending
- 2011-04-25 RU RU2012150281/10A patent/RU2563353C2/ru active
- 2011-04-25 ES ES11716890T patent/ES2755907T3/es active Active
- 2011-04-25 KR KR1020127030708A patent/KR101848945B1/ko active Active
- 2011-04-25 WO PCT/EP2011/056509 patent/WO2011134921A1/en not_active Ceased
- 2011-04-25 JP JP2013506613A patent/JP6113653B2/ja active Active
- 2011-04-25 MX MX2012012525A patent/MX2012012525A/es active IP Right Grant
- 2011-04-25 CN CN201180021025.4A patent/CN102858953B/zh active Active
- 2011-04-25 SG SG2012079158A patent/SG185037A1/en unknown
- 2011-04-25 US US13/695,002 patent/US8986957B2/en active Active
- 2011-04-25 CN CN201510505711.9A patent/CN105331659B/zh active Active
-
2012
- 2012-10-15 IL IL222453A patent/IL222453B/en active IP Right Grant
-
2015
- 2015-02-12 US US14/620,359 patent/US9243224B2/en active Active
- 2015-10-16 JP JP2015204453A patent/JP2016052310A/ja active Pending
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