JP2013524826A5 - - Google Patents
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- Publication number
- JP2013524826A5 JP2013524826A5 JP2013506613A JP2013506613A JP2013524826A5 JP 2013524826 A5 JP2013524826 A5 JP 2013524826A5 JP 2013506613 A JP2013506613 A JP 2013506613A JP 2013506613 A JP2013506613 A JP 2013506613A JP 2013524826 A5 JP2013524826 A5 JP 2013524826A5
- Authority
- JP
- Japan
- Prior art keywords
- cell culture
- culture medium
- mmol
- amino acids
- recombinant polypeptide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims 8
- 239000006143 cell culture medium Substances 0.000 claims 7
- 229940024606 amino acid Drugs 0.000 claims 3
- 235000001014 amino acid Nutrition 0.000 claims 3
- 150000001413 amino acids Chemical class 0.000 claims 3
- 229920001184 polypeptide Polymers 0.000 claims 3
- 102000004196 processed proteins & peptides Human genes 0.000 claims 3
- 108090000765 processed proteins & peptides Proteins 0.000 claims 3
- 239000001763 2-hydroxyethyl(trimethyl)azanium Substances 0.000 claims 2
- 235000019743 Choline chloride Nutrition 0.000 claims 2
- 239000004381 Choline salt Substances 0.000 claims 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims 2
- 229960003178 choline chloride Drugs 0.000 claims 2
- SGMZJAMFUVOLNK-UHFFFAOYSA-M choline chloride Chemical compound [Cl-].C[N+](C)(C)CCO SGMZJAMFUVOLNK-UHFFFAOYSA-M 0.000 claims 2
- 235000019417 choline salt Nutrition 0.000 claims 2
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 claims 2
- 238000004519 manufacturing process Methods 0.000 claims 2
- 239000002609 medium Substances 0.000 claims 2
- 150000003248 quinolines Chemical class 0.000 claims 2
- 239000004475 Arginine Substances 0.000 claims 1
- DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 claims 1
- 239000004471 Glycine Substances 0.000 claims 1
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 claims 1
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 claims 1
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 claims 1
- LEVWYRKDKASIDU-IMJSIDKUSA-N L-cystine Chemical compound [O-]C(=O)[C@@H]([NH3+])CSSC[C@H]([NH3+])C([O-])=O LEVWYRKDKASIDU-IMJSIDKUSA-N 0.000 claims 1
- AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 claims 1
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 claims 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 claims 1
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 claims 1
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 claims 1
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 claims 1
- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 claims 1
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 claims 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 claims 1
- 239000004472 Lysine Substances 0.000 claims 1
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 claims 1
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 claims 1
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 claims 1
- 239000004473 Threonine Substances 0.000 claims 1
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 claims 1
- KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 claims 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 claims 1
- 229960001230 asparagine Drugs 0.000 claims 1
- 235000009582 asparagine Nutrition 0.000 claims 1
- 235000003704 aspartic acid Nutrition 0.000 claims 1
- 238000010923 batch production Methods 0.000 claims 1
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 claims 1
- 210000004027 cell Anatomy 0.000 claims 1
- 210000004978 chinese hamster ovary cell Anatomy 0.000 claims 1
- 229960003067 cystine Drugs 0.000 claims 1
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 claims 1
- AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 claims 1
- 229960000310 isoleucine Drugs 0.000 claims 1
- 229930182817 methionine Natural products 0.000 claims 1
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 claims 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 claims 1
- 239000004474 valine Substances 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US32783710P | 2010-04-26 | 2010-04-26 | |
| US61/327,837 | 2010-04-26 | ||
| PCT/EP2011/056509 WO2011134921A1 (en) | 2010-04-26 | 2011-04-25 | Improved cell culture medium |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015204453A Division JP2016052310A (ja) | 2010-04-26 | 2015-10-16 | 改良型細胞培養培地 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2013524826A JP2013524826A (ja) | 2013-06-20 |
| JP2013524826A5 true JP2013524826A5 (enExample) | 2013-11-28 |
| JP6113653B2 JP6113653B2 (ja) | 2017-04-12 |
Family
ID=44276356
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2013506613A Active JP6113653B2 (ja) | 2010-04-26 | 2011-04-25 | 改良型細胞培養培地 |
| JP2015204453A Pending JP2016052310A (ja) | 2010-04-26 | 2015-10-16 | 改良型細胞培養培地 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015204453A Pending JP2016052310A (ja) | 2010-04-26 | 2015-10-16 | 改良型細胞培養培地 |
Country Status (14)
| Country | Link |
|---|---|
| US (2) | US8986957B2 (enExample) |
| EP (2) | EP3599276A1 (enExample) |
| JP (2) | JP6113653B2 (enExample) |
| KR (1) | KR101848945B1 (enExample) |
| CN (2) | CN105331659B (enExample) |
| AU (1) | AU2011246504B2 (enExample) |
| BR (1) | BR112012027434B1 (enExample) |
| CA (1) | CA2797140C (enExample) |
| ES (1) | ES2755907T3 (enExample) |
| IL (1) | IL222453B (enExample) |
| MX (1) | MX2012012525A (enExample) |
| RU (1) | RU2563353C2 (enExample) |
| SG (1) | SG185037A1 (enExample) |
| WO (1) | WO2011134921A1 (enExample) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8986957B2 (en) | 2010-04-26 | 2015-03-24 | Novartis Ag | Cell culture medium |
| US9976163B2 (en) | 2012-10-03 | 2018-05-22 | Kyowa Hakko Kirin Co., Ltd | Method for preventing reduction of polypeptide by adding amino acid to culture solution |
| CN104822826B (zh) | 2012-10-15 | 2019-04-30 | 百时美施贵宝公司 | 用于蛋白产生的哺乳动物细胞培养过程 |
| CA2943667C (en) * | 2014-01-29 | 2022-11-08 | Lg Life Sciences Ltd. | Method for modulating galactosylation of recombinant protein through optimization of culture medium |
| WO2015155975A1 (ja) * | 2014-04-07 | 2015-10-15 | 東洋製罐グループホールディングス株式会社 | 動物細胞用培養液、及び培養容器 |
| ES2733298T3 (es) | 2014-07-18 | 2019-11-28 | Sandoz Ag | Cuantificación de TNFR2:Fc plegado erróneamente |
| SG11201705743YA (en) * | 2015-04-01 | 2017-08-30 | Boehringer Ingelheim Int | Cell culture medium |
| EP3303555B1 (en) * | 2015-05-29 | 2020-06-24 | Merck Patent GmbH | Deep eutectic solvents and/or ionic liquids in cell culture media |
| EP3303554B1 (en) * | 2015-05-29 | 2020-06-24 | Merck Patent GmbH | Deep eutectic solvents and/or ionic liquids as feed media |
| WO2016207721A1 (en) | 2015-06-25 | 2016-12-29 | University Of Limerick | Mechanical device for generating combinatorial library |
| JP2018536404A (ja) * | 2015-11-09 | 2018-12-13 | ブリストル−マイヤーズ スクイブ カンパニーＢｒｉｓｔｏｌ−Ｍｙｅｒｓ Ｓｑｕｉｂｂ Ｃｏｍｐａｎｙ | Ｃｈｏ細胞において産生したポリペプチドの品質特性を操作する方法 |
| KR20230124093A (ko) * | 2016-05-10 | 2023-08-24 | 제넨테크, 인크. | 폴리펩타이드의 재조합 생산 동안 트리설파이드 결합을감소시키기 위한 방법 |
| KR102893388B1 (ko) * | 2016-10-31 | 2025-11-28 | 삼성바이오에피스 주식회사 | 세포 배양 배지에서 원치 않는 배양 부산물을 감소시키는 공정 |
| CN106676058B (zh) * | 2016-12-09 | 2020-06-19 | 中农威特生物科技股份有限公司 | 一种bhk21悬浮细胞高密度流加培养方法及其在口蹄疫病毒增殖中的应用 |
| GB201708655D0 (en) * | 2017-05-31 | 2017-07-12 | Ucb Biopharma Sprl | Cell culture methods |
| EP4056678A4 (en) * | 2019-11-05 | 2023-12-06 | Ajinomoto Co., Inc. | Method for producing protein |
| CA3167733A1 (en) * | 2020-01-14 | 2021-07-22 | Ajinomoto Co., Inc. | High-density cell culture method |
Family Cites Families (37)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US4767704A (en) * | 1983-10-07 | 1988-08-30 | Columbia University In The City Of New York | Protein-free culture medium |
| US6048728A (en) * | 1988-09-23 | 2000-04-11 | Chiron Corporation | Cell culture medium for enhanced cell growth, culture longevity, and product expression |
| US5266479A (en) * | 1989-04-12 | 1993-11-30 | Board Of Regents, The University Of Texas System | High calcium chemically defined culture medium |
| US5122469A (en) | 1990-10-03 | 1992-06-16 | Genentech, Inc. | Method for culturing Chinese hamster ovary cells to improve production of recombinant proteins |
| WO1992015670A1 (en) * | 1991-02-28 | 1992-09-17 | Hyclone Laboratories, Inc. | Blended bovine sera cellular growth media and their methods of production and use |
| GB9118664D0 (en) | 1991-08-30 | 1991-10-16 | Celltech Ltd | Cell culture |
| USH1532H (en) * | 1993-11-03 | 1996-05-07 | Genetics Institute, Inc. | Adaption of mammalian cell lines to high cell densities |
| US5856179A (en) | 1994-03-10 | 1999-01-05 | Genentech, Inc. | Polypeptide production in animal cell culture |
| AU2260097A (en) * | 1996-03-12 | 1997-10-01 | Life Technologies, Inc. | Hematopoietic cell culture nutrient supplement |
| EP2243827B2 (en) | 1996-08-30 | 2017-11-22 | Life Technologies Corporation | Serum-free mammalian cell culture medium, and uses thereof |
| AU4751697A (en) | 1996-10-10 | 1998-05-05 | Douglas Danner | Animal cell culture media comprising plant-derived nutrients |
| US6692961B1 (en) * | 1996-10-11 | 2004-02-17 | Invitrogen Corporation | Defined systems for epithelial cell culture and use thereof |
| US20060286668A1 (en) * | 1999-04-30 | 2006-12-21 | Invitrogen Corporation | Animal-cell culture media comprising non-animal or plant-derived nutrients |
| EP1210410B1 (en) | 1999-08-27 | 2008-01-16 | Invitrogen Corporation | Metal binding compounds and their use in cell culture medium compositions |
| US20030096414A1 (en) | 2001-03-27 | 2003-05-22 | Invitrogen Corporation | Culture medium for cell growth and transfection |
| CA2447791A1 (en) * | 2001-06-13 | 2002-12-19 | Neslihan Delacruz | Methods of culturing animal cells and polypeptide production in animal cells |
| ES2291534T3 (es) | 2001-10-02 | 2008-03-01 | Novo Nordisk Health Care Ag | Metodo para la produccion de proteinas recombinantes en celulas eucariotas. |
| CA2542121A1 (en) | 2003-10-09 | 2005-04-21 | Kyowa Hakko Kogyo Co., Ltd. | Genomically modified cell neutralized to serum-free system |
| EP1673452B1 (en) | 2003-10-10 | 2015-12-23 | Novo Nordisk Health Care AG | Method for large-scale production of a polypeptide in eukaryote cells |
| US7294484B2 (en) | 2004-08-27 | 2007-11-13 | Wyeth Research Ireland Limited | Production of polypeptides |
| TWI374935B (en) | 2004-08-27 | 2012-10-21 | Pfizer Ireland Pharmaceuticals | Production of α-abeta |
| US7300773B2 (en) | 2004-08-27 | 2007-11-27 | Wyeth Research Ireland Limited | Production of TNFR-Ig |
| JP2008518597A (ja) * | 2004-10-29 | 2008-06-05 | セントカー・インコーポレーテツド | 化学的規定培地組成物 |
| WO2006108455A1 (en) * | 2004-11-02 | 2006-10-19 | Ares Trading S.A. | Serum-free cell culture medium for mammalian cells |
| TWI369401B (en) | 2005-07-05 | 2012-08-01 | Ares Trading Sa | Serum-free culture medium for the production of recombinant gonadotropins |
| US20070231895A1 (en) | 2005-11-02 | 2007-10-04 | Lee Gene W | Methods for adapting mammalian cells |
| ES2474573T3 (es) | 2006-01-04 | 2014-07-09 | Baxter International Inc | Medio de cultivo celular sin oligop�ptidos |
| CA2666317C (en) | 2006-11-03 | 2013-08-06 | Wyeth | Glycolysis-inhibiting substances in cell culture |
| FR2910024B1 (fr) | 2006-12-19 | 2009-03-27 | Agronomique Inst Nat Rech | Milieu de culture synthetique chimiquement defini. |
| CA2687082C (en) | 2007-05-11 | 2014-01-14 | Amgen Inc. | Improved feed media |
| US8771988B2 (en) | 2007-10-12 | 2014-07-08 | Hoffmann-La Roche Inc. | Protein expression from multiple nucleic acids |
| JP2011516086A (ja) * | 2008-04-18 | 2011-05-26 | 上海中信国健薬業股▲ふん▼有限公司 | 濃縮培養液及びその使用方法 |
| CN101381702A (zh) * | 2008-09-10 | 2009-03-11 | 吉林省赛达生物科技有限公司 | 无动物组分细胞培养基 |
| JP2012503487A (ja) * | 2008-09-26 | 2012-02-09 | シェーリング コーポレイション | 高力価抗体の製造 |
| ES2533074T3 (es) | 2010-04-26 | 2015-04-07 | Novartis Ag | Medio de cultivo celular mejorado |
| US8986957B2 (en) | 2010-04-26 | 2015-03-24 | Novartis Ag | Cell culture medium |
| EP3862423A1 (en) | 2010-04-26 | 2021-08-11 | Novartis AG | Improved cell cultivation process |
-
2011
- 2011-04-25 US US13/695,002 patent/US8986957B2/en active Active
- 2011-04-25 ES ES11716890T patent/ES2755907T3/es active Active
- 2011-04-25 WO PCT/EP2011/056509 patent/WO2011134921A1/en not_active Ceased
- 2011-04-25 AU AU2011246504A patent/AU2011246504B2/en active Active
- 2011-04-25 RU RU2012150281/10A patent/RU2563353C2/ru active
- 2011-04-25 EP EP19192479.4A patent/EP3599276A1/en active Pending
- 2011-04-25 CN CN201510505711.9A patent/CN105331659B/zh active Active
- 2011-04-25 CA CA2797140A patent/CA2797140C/en active Active
- 2011-04-25 SG SG2012079158A patent/SG185037A1/en unknown
- 2011-04-25 JP JP2013506613A patent/JP6113653B2/ja active Active
- 2011-04-25 EP EP11716890.6A patent/EP2563903B1/en active Active
- 2011-04-25 MX MX2012012525A patent/MX2012012525A/es active IP Right Grant
- 2011-04-25 BR BR112012027434-1A patent/BR112012027434B1/pt active IP Right Grant
- 2011-04-25 KR KR1020127030708A patent/KR101848945B1/ko active Active
- 2011-04-25 CN CN201180021025.4A patent/CN102858953B/zh active Active
-
2012
- 2012-10-15 IL IL222453A patent/IL222453B/en active IP Right Grant
-
2015
- 2015-02-12 US US14/620,359 patent/US9243224B2/en active Active
- 2015-10-16 JP JP2015204453A patent/JP2016052310A/ja active Pending
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