SG185037A1 - Improved cell culture medium - Google Patents
Improved cell culture medium Download PDFInfo
- Publication number
- SG185037A1 SG185037A1 SG2012079158A SG2012079158A SG185037A1 SG 185037 A1 SG185037 A1 SG 185037A1 SG 2012079158 A SG2012079158 A SG 2012079158A SG 2012079158 A SG2012079158 A SG 2012079158A SG 185037 A1 SG185037 A1 SG 185037A1
- Authority
- SG
- Singapore
- Prior art keywords
- cell culture
- choline
- production
- polypeptide
- medium
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Classifications
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
- C12N5/005—Protein-free medium
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
- C12N5/0031—Serum-free culture media
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
- C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/32—Amino acids
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/38—Vitamins
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2500/00—Specific components of cell culture medium
- C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
- C12N2500/95—Protein-free medium and culture conditions
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2500/00—Specific components of cell culture medium
- C12N2500/99—Serum-free medium
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
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- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
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| US32783710P | 2010-04-26 | 2010-04-26 | |
| PCT/EP2011/056509 WO2011134921A1 (en) | 2010-04-26 | 2011-04-25 | Improved cell culture medium |
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| SG185037A1 true SG185037A1 (en) | 2012-11-29 |
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| US8986957B2 (en) | 2010-04-26 | 2015-03-24 | Novartis Ag | Cell culture medium |
| US9976163B2 (en) | 2012-10-03 | 2018-05-22 | Kyowa Hakko Kirin Co., Ltd | Method for preventing reduction of polypeptide by adding amino acid to culture solution |
| CN104822826B (zh) | 2012-10-15 | 2019-04-30 | 百时美施贵宝公司 | 用于蛋白产生的哺乳动物细胞培养过程 |
| CA2943667C (en) * | 2014-01-29 | 2022-11-08 | Lg Life Sciences Ltd. | Method for modulating galactosylation of recombinant protein through optimization of culture medium |
| WO2015155975A1 (ja) * | 2014-04-07 | 2015-10-15 | 東洋製罐グループホールディングス株式会社 | 動物細胞用培養液、及び培養容器 |
| ES2733298T3 (es) | 2014-07-18 | 2019-11-28 | Sandoz Ag | Cuantificación de TNFR2:Fc plegado erróneamente |
| SG11201705743YA (en) * | 2015-04-01 | 2017-08-30 | Boehringer Ingelheim Int | Cell culture medium |
| EP3303555B1 (en) * | 2015-05-29 | 2020-06-24 | Merck Patent GmbH | Deep eutectic solvents and/or ionic liquids in cell culture media |
| EP3303554B1 (en) * | 2015-05-29 | 2020-06-24 | Merck Patent GmbH | Deep eutectic solvents and/or ionic liquids as feed media |
| WO2016207721A1 (en) | 2015-06-25 | 2016-12-29 | University Of Limerick | Mechanical device for generating combinatorial library |
| JP2018536404A (ja) * | 2015-11-09 | 2018-12-13 | ブリストル−マイヤーズ スクイブ カンパニーＢｒｉｓｔｏｌ−Ｍｙｅｒｓ Ｓｑｕｉｂｂ Ｃｏｍｐａｎｙ | Ｃｈｏ細胞において産生したポリペプチドの品質特性を操作する方法 |
| KR20230124093A (ko) * | 2016-05-10 | 2023-08-24 | 제넨테크, 인크. | 폴리펩타이드의 재조합 생산 동안 트리설파이드 결합을감소시키기 위한 방법 |
| KR102893388B1 (ko) * | 2016-10-31 | 2025-11-28 | 삼성바이오에피스 주식회사 | 세포 배양 배지에서 원치 않는 배양 부산물을 감소시키는 공정 |
| CN106676058B (zh) * | 2016-12-09 | 2020-06-19 | 中农威特生物科技股份有限公司 | 一种bhk21悬浮细胞高密度流加培养方法及其在口蹄疫病毒增殖中的应用 |
| GB201708655D0 (en) * | 2017-05-31 | 2017-07-12 | Ucb Biopharma Sprl | Cell culture methods |
| EP4056678A4 (en) * | 2019-11-05 | 2023-12-06 | Ajinomoto Co., Inc. | Method for producing protein |
| CA3167733A1 (en) * | 2020-01-14 | 2021-07-22 | Ajinomoto Co., Inc. | High-density cell culture method |
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| US4767704A (en) * | 1983-10-07 | 1988-08-30 | Columbia University In The City Of New York | Protein-free culture medium |
| US6048728A (en) * | 1988-09-23 | 2000-04-11 | Chiron Corporation | Cell culture medium for enhanced cell growth, culture longevity, and product expression |
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| US5122469A (en) | 1990-10-03 | 1992-06-16 | Genentech, Inc. | Method for culturing Chinese hamster ovary cells to improve production of recombinant proteins |
| WO1992015670A1 (en) * | 1991-02-28 | 1992-09-17 | Hyclone Laboratories, Inc. | Blended bovine sera cellular growth media and their methods of production and use |
| GB9118664D0 (en) | 1991-08-30 | 1991-10-16 | Celltech Ltd | Cell culture |
| USH1532H (en) * | 1993-11-03 | 1996-05-07 | Genetics Institute, Inc. | Adaption of mammalian cell lines to high cell densities |
| US5856179A (en) | 1994-03-10 | 1999-01-05 | Genentech, Inc. | Polypeptide production in animal cell culture |
| AU2260097A (en) * | 1996-03-12 | 1997-10-01 | Life Technologies, Inc. | Hematopoietic cell culture nutrient supplement |
| EP2243827B2 (en) | 1996-08-30 | 2017-11-22 | Life Technologies Corporation | Serum-free mammalian cell culture medium, and uses thereof |
| AU4751697A (en) | 1996-10-10 | 1998-05-05 | Douglas Danner | Animal cell culture media comprising plant-derived nutrients |
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| US20060286668A1 (en) * | 1999-04-30 | 2006-12-21 | Invitrogen Corporation | Animal-cell culture media comprising non-animal or plant-derived nutrients |
| EP1210410B1 (en) | 1999-08-27 | 2008-01-16 | Invitrogen Corporation | Metal binding compounds and their use in cell culture medium compositions |
| US20030096414A1 (en) | 2001-03-27 | 2003-05-22 | Invitrogen Corporation | Culture medium for cell growth and transfection |
| CA2447791A1 (en) * | 2001-06-13 | 2002-12-19 | Neslihan Delacruz | Methods of culturing animal cells and polypeptide production in animal cells |
| ES2291534T3 (es) | 2001-10-02 | 2008-03-01 | Novo Nordisk Health Care Ag | Metodo para la produccion de proteinas recombinantes en celulas eucariotas. |
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| EP1673452B1 (en) | 2003-10-10 | 2015-12-23 | Novo Nordisk Health Care AG | Method for large-scale production of a polypeptide in eukaryote cells |
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| JP2008518597A (ja) * | 2004-10-29 | 2008-06-05 | セントカー・インコーポレーテツド | 化学的規定培地組成物 |
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| TWI369401B (en) | 2005-07-05 | 2012-08-01 | Ares Trading Sa | Serum-free culture medium for the production of recombinant gonadotropins |
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| ES2474573T3 (es) | 2006-01-04 | 2014-07-09 | Baxter International Inc | Medio de cultivo celular sin oligop�ptidos |
| CA2666317C (en) | 2006-11-03 | 2013-08-06 | Wyeth | Glycolysis-inhibiting substances in cell culture |
| FR2910024B1 (fr) | 2006-12-19 | 2009-03-27 | Agronomique Inst Nat Rech | Milieu de culture synthetique chimiquement defini. |
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| US8771988B2 (en) | 2007-10-12 | 2014-07-08 | Hoffmann-La Roche Inc. | Protein expression from multiple nucleic acids |
| JP2011516086A (ja) * | 2008-04-18 | 2011-05-26 | 上海中信国健薬業股▲ふん▼有限公司 | 濃縮培養液及びその使用方法 |
| CN101381702A (zh) * | 2008-09-10 | 2009-03-11 | 吉林省赛达生物科技有限公司 | 无动物组分细胞培养基 |
| JP2012503487A (ja) * | 2008-09-26 | 2012-02-09 | シェーリング コーポレイション | 高力価抗体の製造 |
| ES2533074T3 (es) | 2010-04-26 | 2015-04-07 | Novartis Ag | Medio de cultivo celular mejorado |
| US8986957B2 (en) | 2010-04-26 | 2015-03-24 | Novartis Ag | Cell culture medium |
| EP3862423A1 (en) | 2010-04-26 | 2021-08-11 | Novartis AG | Improved cell cultivation process |
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| RU2012150281A (ru) | 2014-06-10 |
| US20130102032A1 (en) | 2013-04-25 |
| IL222453B (en) | 2018-11-29 |
| JP6113653B2 (ja) | 2017-04-12 |
| BR112012027434A2 (pt) | 2015-09-22 |
| KR20130098869A (ko) | 2013-09-05 |
| AU2011246504B2 (en) | 2013-09-26 |
| CN102858953B (zh) | 2015-09-09 |
| CN105331659B (zh) | 2019-07-16 |
| ES2755907T3 (es) | 2020-04-24 |
| EP3599276A1 (en) | 2020-01-29 |
| US9243224B2 (en) | 2016-01-26 |
| KR101848945B1 (ko) | 2018-04-16 |
| EP2563903B1 (en) | 2019-08-21 |
| CA2797140C (en) | 2018-01-23 |
| IL222453A0 (en) | 2012-12-31 |
| JP2016052310A (ja) | 2016-04-14 |
| CA2797140A1 (en) | 2011-11-03 |
| AU2011246504A1 (en) | 2012-11-22 |
| MX2012012525A (es) | 2012-11-23 |
| US20150218509A1 (en) | 2015-08-06 |
| JP2013524826A (ja) | 2013-06-20 |
| CN102858953A (zh) | 2013-01-02 |
| US8986957B2 (en) | 2015-03-24 |
| BR112012027434B1 (pt) | 2020-09-29 |
| CN105331659A (zh) | 2016-02-17 |
| WO2011134921A1 (en) | 2011-11-03 |
| RU2563353C2 (ru) | 2015-09-20 |
| EP2563903A1 (en) | 2013-03-06 |
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