BR112012027434A2 - meio de cultura de células melhorado - Google Patents

meio de cultura de células melhorado

Info

Publication number
BR112012027434A2
BR112012027434A2 BR112012027434A BR112012027434A BR112012027434A2 BR 112012027434 A2 BR112012027434 A2 BR 112012027434A2 BR 112012027434 A BR112012027434 A BR 112012027434A BR 112012027434 A BR112012027434 A BR 112012027434A BR 112012027434 A2 BR112012027434 A2 BR 112012027434A2
Authority
BR
Brazil
Prior art keywords
cell culture
culture medium
improved cell
culture media
amino acids
Prior art date
Application number
BR112012027434A
Other languages
English (en)
Other versions
BR112012027434B1 (pt
Inventor
Helene C Chassin
Kerstin Dorsch
Wolfgang Ernst Gustav Budach
Original Assignee
Novartis Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=44276356&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=BR112012027434(A2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Novartis Ag filed Critical Novartis Ag
Publication of BR112012027434A2 publication Critical patent/BR112012027434A2/pt
Publication of BR112012027434B1 publication Critical patent/BR112012027434B1/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0031—Serum-free culture media
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/005—Protein-free medium
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C12N2500/95—Protein-free medium and culture conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/99—Serum-free medium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

meio de cultura de células melhorado. a presente invenção refere-se a um meio de cultura de células com alto teor de cloreto de colina. os meios de cultura de células também compreendem apenas quantidades moderadas de aminoácidos, em particula, a quantidade de glutamina nos meios de cultura de células é limitada. os meios de cultura de células com alto teor de cloreto de colina são particulamente adequados para cultura de células descotínua alimentada, pelo que as viabilidades das células permanecem a um nível mais elevado por um tempo maior e altas titulações de polipeptídeo, embora sejam usadas quantidades limitadas de aminoácidos.
BR112012027434-1A 2010-04-26 2011-04-25 Processo para produção de um polipeptídeo recombinante compreendendo uma fase de produção BR112012027434B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US32783710P 2010-04-26 2010-04-26
US61/327.837 2010-04-26
PCT/EP2011/056509 WO2011134921A1 (en) 2010-04-26 2011-04-25 Improved cell culture medium

Publications (2)

Publication Number Publication Date
BR112012027434A2 true BR112012027434A2 (pt) 2015-09-22
BR112012027434B1 BR112012027434B1 (pt) 2020-09-29

Family

ID=44276356

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112012027434-1A BR112012027434B1 (pt) 2010-04-26 2011-04-25 Processo para produção de um polipeptídeo recombinante compreendendo uma fase de produção

Country Status (14)

Country Link
US (2) US8986957B2 (pt)
EP (2) EP3599276A1 (pt)
JP (2) JP6113653B2 (pt)
KR (1) KR101848945B1 (pt)
CN (2) CN102858953B (pt)
AU (1) AU2011246504B2 (pt)
BR (1) BR112012027434B1 (pt)
CA (1) CA2797140C (pt)
ES (1) ES2755907T3 (pt)
IL (1) IL222453B (pt)
MX (1) MX2012012525A (pt)
RU (1) RU2563353C2 (pt)
SG (1) SG185037A1 (pt)
WO (1) WO2011134921A1 (pt)

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WO2014062535A1 (en) 2012-10-15 2014-04-24 Bristol-Myers Squibb Company Mammalian cell culture processes for protein production
RU2642285C1 (ru) * 2014-01-29 2018-01-24 ЭлДжи КЕМ, ЛТД. Способ получения целевого антитела с модулированным галактозилированием (варианты) и способ модулирования галактозилирования целевого антитела (варианты) путем оптимизации культуральной среды
JPWO2015155975A1 (ja) * 2014-04-07 2017-04-13 東洋製罐グループホールディングス株式会社 動物細胞用培養液、及び培養容器
EP2975050B1 (en) 2014-07-18 2019-05-29 Sandoz Ag Quantification of misfolded TNFR2:Fc
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DK3303554T3 (da) * 2015-05-29 2020-08-31 Merck Patent Gmbh Dybe eutektiske opløsningsmidler og/eller ioniske væsker som fodringsmedie
JP2018529309A (ja) 2015-06-25 2018-10-11 ユニバーシティ・オブ・リムリック 組み合わせライブラリを生成するための機械的デバイス
CN108350416A (zh) * 2015-11-09 2018-07-31 百时美施贵宝公司 操纵在cho细胞中产生的多肽的品质属性的方法
KR20190005966A (ko) * 2016-05-10 2019-01-16 제넨테크, 인크. 폴리펩타이드의 재조합 생산 동안 트리설파이드 결합을 감소시키기 위한 방법
KR20180047404A (ko) * 2016-10-31 2018-05-10 삼성바이오에피스 주식회사 세포 배양 배지에서 원치 않는 배양 부산물을 감소시키는 공정
CN106676058B (zh) * 2016-12-09 2020-06-19 中农威特生物科技股份有限公司 一种bhk21悬浮细胞高密度流加培养方法及其在口蹄疫病毒增殖中的应用
GB201708655D0 (en) * 2017-05-31 2017-07-12 Ucb Biopharma Sprl Cell culture methods
JPWO2021090888A1 (pt) * 2019-11-05 2021-05-14
CN115003792A (zh) * 2020-01-14 2022-09-02 味之素株式会社 细胞的高密度培养方法

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Also Published As

Publication number Publication date
JP6113653B2 (ja) 2017-04-12
CA2797140C (en) 2018-01-23
EP2563903A1 (en) 2013-03-06
CA2797140A1 (en) 2011-11-03
CN102858953B (zh) 2015-09-09
US8986957B2 (en) 2015-03-24
CN105331659B (zh) 2019-07-16
US20150218509A1 (en) 2015-08-06
IL222453A0 (en) 2012-12-31
RU2012150281A (ru) 2014-06-10
JP2016052310A (ja) 2016-04-14
CN102858953A (zh) 2013-01-02
KR20130098869A (ko) 2013-09-05
EP2563903B1 (en) 2019-08-21
AU2011246504B2 (en) 2013-09-26
WO2011134921A1 (en) 2011-11-03
JP2013524826A (ja) 2013-06-20
AU2011246504A1 (en) 2012-11-22
SG185037A1 (en) 2012-11-29
US20130102032A1 (en) 2013-04-25
RU2563353C2 (ru) 2015-09-20
MX2012012525A (es) 2012-11-23
CN105331659A (zh) 2016-02-17
IL222453B (en) 2018-11-29
EP3599276A1 (en) 2020-01-29
US9243224B2 (en) 2016-01-26
KR101848945B1 (ko) 2018-04-16
BR112012027434B1 (pt) 2020-09-29
ES2755907T3 (es) 2020-04-24

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B09A Decision: intention to grant [chapter 9.1 patent gazette]
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