CN105331659B - 改进的细胞培养基 - Google Patents

改进的细胞培养基 Download PDF

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CN105331659B
CN105331659B CN201510505711.9A CN201510505711A CN105331659B CN 105331659 B CN105331659 B CN 105331659B CN 201510505711 A CN201510505711 A CN 201510505711A CN 105331659 B CN105331659 B CN 105331659B
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culture medium
cell
cell culture
polypeptide
medium
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CN105331659A (zh
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W·E·G·布达施
H·C·沙桑
K·多尔施
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Novartis AG
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    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018Culture media for cell or tissue culture
    • C12N5/0031Serum-free culture media
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018Culture media for cell or tissue culture
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018Culture media for cell or tissue culture
    • C12N5/005Protein-free medium
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/10Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14Specific host cells or culture conditions, e.g. components, pH or temperature
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    • C12N2500/00Specific components of cell culture medium
    • C12N2500/30Organic components
    • C12N2500/32Amino acids
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2500/00Specific components of cell culture medium
    • C12N2500/30Organic components
    • C12N2500/38Vitamins
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    • C12N2500/00Specific components of cell culture medium
    • C12N2500/90Serum-free medium, which may still contain naturally-sourced components
    • C12N2500/95Protein-free medium and culture conditions
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    • C12N2500/00Specific components of cell culture medium
    • C12N2500/99Serum-free medium
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    • C12N2510/00Genetically modified cells
    • C12N2510/02Cells for production

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
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  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201510505711.9A 2010-04-26 2011-04-25 改进的细胞培养基 Active CN105331659B (zh)

Applications Claiming Priority (3)

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US32783710P 2010-04-26 2010-04-26
US61/327,837 2010-04-26
CN201180021025.4A CN102858953B (zh) 2010-04-26 2011-04-25 改进的细胞培养基

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CN105331659A CN105331659A (zh) 2016-02-17
CN105331659B true CN105331659B (zh) 2019-07-16

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US (2) US8986957B2 (enExample)
EP (2) EP2563903B1 (enExample)
JP (2) JP6113653B2 (enExample)
KR (1) KR101848945B1 (enExample)
CN (2) CN102858953B (enExample)
AU (1) AU2011246504B2 (enExample)
BR (1) BR112012027434B1 (enExample)
CA (1) CA2797140C (enExample)
ES (1) ES2755907T3 (enExample)
IL (1) IL222453B (enExample)
MX (1) MX2012012525A (enExample)
RU (1) RU2563353C2 (enExample)
SG (1) SG185037A1 (enExample)
WO (1) WO2011134921A1 (enExample)

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MX2012012525A (es) 2010-04-26 2012-11-23 Novartis Ag Medio de cultivo mejorado.
WO2014054744A1 (ja) * 2012-10-03 2014-04-10 協和発酵キリン株式会社 培養液にアミノ酸を添加することによるポリペプチドの還元防止方法
JP2015531244A (ja) 2012-10-15 2015-11-02 ブリストル−マイヤーズ スクイブ カンパニーBristol−Myers Squibb Company タンパク質を製造するための哺乳動物細胞培養方法
KR102255902B1 (ko) * 2014-01-29 2021-05-26 주식회사 엘지화학 배양배지의 최적화를 통한 재조합 단백질의 갈락토오스화를 조절하는 방법
CN106062174A (zh) * 2014-04-07 2016-10-26 东洋制罐集团控股株式会社 动物细胞用培养液、以及培养容器
EP2975050B1 (en) 2014-07-18 2019-05-29 Sandoz Ag Quantification of misfolded TNFR2:Fc
EP3277797B1 (en) * 2015-04-01 2020-08-19 Boehringer Ingelheim International GmbH Cell culture medium
US10731126B2 (en) 2015-05-29 2020-08-04 Merck Patent Gmbh Deep eutectic solvents and/or ionic liquids in cell culture media
EP3303554B1 (en) * 2015-05-29 2020-06-24 Merck Patent GmbH Deep eutectic solvents and/or ionic liquids as feed media
WO2016207721A1 (en) 2015-06-25 2016-12-29 University Of Limerick Mechanical device for generating combinatorial library
KR20180073693A (ko) * 2015-11-09 2018-07-02 브리스톨-마이어스 스큅 컴퍼니 Cho 세포에서 생산되는 폴리펩티드의 품질 속성을 조작하는 방법
CN114703244A (zh) * 2016-05-10 2022-07-05 豪夫迈·罗氏有限公司 重组产生多肽期间减少三硫键的方法
KR102893388B1 (ko) * 2016-10-31 2025-11-28 삼성바이오에피스 주식회사 세포 배양 배지에서 원치 않는 배양 부산물을 감소시키는 공정
CN106676058B (zh) * 2016-12-09 2020-06-19 中农威特生物科技股份有限公司 一种bhk21悬浮细胞高密度流加培养方法及其在口蹄疫病毒增殖中的应用
GB201708655D0 (en) * 2017-05-31 2017-07-12 Ucb Biopharma Sprl Cell culture methods
KR20220097421A (ko) 2019-11-05 2022-07-07 아지노모토 가부시키가이샤 단백질의 제조 방법
CN115003792A (zh) * 2020-01-14 2022-09-02 味之素株式会社 细胞的高密度培养方法

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CN101052708A (zh) * 2004-11-02 2007-10-10 阿雷斯贸易股份有限公司 哺乳动物细胞的无血清细胞培养基
CN101381702A (zh) * 2008-09-10 2009-03-11 吉林省赛达生物科技有限公司 无动物组分细胞培养基

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CN101052708A (zh) * 2004-11-02 2007-10-10 阿雷斯贸易股份有限公司 哺乳动物细胞的无血清细胞培养基
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Also Published As

Publication number Publication date
CN102858953B (zh) 2015-09-09
US20130102032A1 (en) 2013-04-25
EP2563903A1 (en) 2013-03-06
MX2012012525A (es) 2012-11-23
CA2797140C (en) 2018-01-23
CA2797140A1 (en) 2011-11-03
BR112012027434B1 (pt) 2020-09-29
CN102858953A (zh) 2013-01-02
EP2563903B1 (en) 2019-08-21
KR20130098869A (ko) 2013-09-05
US9243224B2 (en) 2016-01-26
JP2016052310A (ja) 2016-04-14
KR101848945B1 (ko) 2018-04-16
EP3599276A1 (en) 2020-01-29
WO2011134921A1 (en) 2011-11-03
IL222453B (en) 2018-11-29
RU2563353C2 (ru) 2015-09-20
JP2013524826A (ja) 2013-06-20
IL222453A0 (en) 2012-12-31
CN105331659A (zh) 2016-02-17
AU2011246504B2 (en) 2013-09-26
ES2755907T3 (es) 2020-04-24
US20150218509A1 (en) 2015-08-06
US8986957B2 (en) 2015-03-24
BR112012027434A2 (pt) 2015-09-22
AU2011246504A1 (en) 2012-11-22
SG185037A1 (en) 2012-11-29
RU2012150281A (ru) 2014-06-10
JP6113653B2 (ja) 2017-04-12

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