CN102858953B - 改进的细胞培养基 - Google Patents

改进的细胞培养基 Download PDF

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Publication number
CN102858953B
CN102858953B CN201180021025.4A CN201180021025A CN102858953B CN 102858953 B CN102858953 B CN 102858953B CN 201180021025 A CN201180021025 A CN 201180021025A CN 102858953 B CN102858953 B CN 102858953B
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cell culture
medium
culture medium
production
choline
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CN102858953A (zh
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W·E·G·布达施
H·C·沙桑
K·多尔施
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Novartis AG
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Novartis AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/005—Protein-free medium
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0031—Serum-free culture media
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C12N2500/95—Protein-free medium and culture conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/99—Serum-free medium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201180021025.4A 2010-04-26 2011-04-25 改进的细胞培养基 Active CN102858953B (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201510505711.9A CN105331659B (zh) 2010-04-26 2011-04-25 改进的细胞培养基

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US32783710P 2010-04-26 2010-04-26
US61/327,837 2010-04-26
PCT/EP2011/056509 WO2011134921A1 (en) 2010-04-26 2011-04-25 Improved cell culture medium

Related Child Applications (1)

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CN102858953A CN102858953A (zh) 2013-01-02
CN102858953B true CN102858953B (zh) 2015-09-09

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CN201180021025.4A Active CN102858953B (zh) 2010-04-26 2011-04-25 改进的细胞培养基

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US (2) US8986957B2 (enExample)
EP (2) EP3599276A1 (enExample)
JP (2) JP6113653B2 (enExample)
KR (1) KR101848945B1 (enExample)
CN (2) CN105331659B (enExample)
AU (1) AU2011246504B2 (enExample)
BR (1) BR112012027434B1 (enExample)
CA (1) CA2797140C (enExample)
ES (1) ES2755907T3 (enExample)
IL (1) IL222453B (enExample)
MX (1) MX2012012525A (enExample)
RU (1) RU2563353C2 (enExample)
SG (1) SG185037A1 (enExample)
WO (1) WO2011134921A1 (enExample)

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CA2797140C (en) 2010-04-26 2018-01-23 Novartis Ag Improved cell culture medium
JP6411891B2 (ja) * 2012-10-03 2018-10-24 協和発酵キリン株式会社 培養液にアミノ酸を添加することによるポリペプチドの還元防止方法
ES2633960T3 (es) 2012-10-15 2017-09-26 Bristol-Myers Squibb Company Procesos de cultivo de células de mamífero para la producción de proteínas
AU2015211514B2 (en) * 2014-01-29 2018-05-10 Lg Chem, Ltd. Method for modulating galactosylation of recombinant protein through optimization of culture medium
CN106062174A (zh) * 2014-04-07 2016-10-26 东洋制罐集团控股株式会社 动物细胞用培养液、以及培养容器
ES2733298T3 (es) 2014-07-18 2019-11-28 Sandoz Ag Cuantificación de TNFR2:Fc plegado erróneamente
US11505784B2 (en) * 2015-04-01 2022-11-22 Boehringer Ingelheim International Gmbh Cell culture medium
WO2016192829A1 (en) * 2015-05-29 2016-12-08 Merck Patent Gmbh Deep eutectic solvents and/or ionic liquids as feed media
DK3303555T3 (da) 2015-05-29 2020-08-31 Merck Patent Gmbh Dybe eutektiske opløsningsmidler og/eller ioniske væsker i celledyrkningsmedium
JP2018529309A (ja) 2015-06-25 2018-10-11 ユニバーシティ・オブ・リムリック 組み合わせライブラリを生成するための機械的デバイス
EP3374493A1 (en) 2015-11-09 2018-09-19 Bristol-Myers Squibb Company Methods to manipulate quality attributes of polypeptides produced in cho cells
BR112018073133A2 (pt) * 2016-05-10 2019-04-30 Genentech Inc métodos de diminuição das ligações trissulfeto durante a produção recombinante de polipeptídeos
KR102893388B1 (ko) * 2016-10-31 2025-11-28 삼성바이오에피스 주식회사 세포 배양 배지에서 원치 않는 배양 부산물을 감소시키는 공정
CN106676058B (zh) * 2016-12-09 2020-06-19 中农威特生物科技股份有限公司 一种bhk21悬浮细胞高密度流加培养方法及其在口蹄疫病毒增殖中的应用
GB201708655D0 (en) * 2017-05-31 2017-07-12 Ucb Biopharma Sprl Cell culture methods
WO2021090888A1 (ja) 2019-11-05 2021-05-14 味の素株式会社 タンパク質の製造方法
CN115003792A (zh) * 2020-01-14 2022-09-02 味之素株式会社 细胞的高密度培养方法

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WO1995012664A1 (en) * 1993-11-03 1995-05-11 Genetics Institute, Inc. Adaption of mammalian cell lines to high cell densities
US6048728A (en) * 1988-09-23 2000-04-11 Chiron Corporation Cell culture medium for enhanced cell growth, culture longevity, and product expression
CN101052708A (zh) * 2004-11-02 2007-10-10 阿雷斯贸易股份有限公司 哺乳动物细胞的无血清细胞培养基
CN101381702A (zh) * 2008-09-10 2009-03-11 吉林省赛达生物科技有限公司 无动物组分细胞培养基
WO2009127098A1 (zh) * 2008-04-18 2009-10-22 上海中信国健药业有限公司 一种浓缩培养液及其使用方法

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US6048728A (en) * 1988-09-23 2000-04-11 Chiron Corporation Cell culture medium for enhanced cell growth, culture longevity, and product expression
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CN101052708A (zh) * 2004-11-02 2007-10-10 阿雷斯贸易股份有限公司 哺乳动物细胞的无血清细胞培养基
WO2009127098A1 (zh) * 2008-04-18 2009-10-22 上海中信国健药业有限公司 一种浓缩培养液及其使用方法
CN101381702A (zh) * 2008-09-10 2009-03-11 吉林省赛达生物科技有限公司 无动物组分细胞培养基

Also Published As

Publication number Publication date
US20150218509A1 (en) 2015-08-06
EP3599276A1 (en) 2020-01-29
CN102858953A (zh) 2013-01-02
RU2012150281A (ru) 2014-06-10
ES2755907T3 (es) 2020-04-24
CN105331659B (zh) 2019-07-16
IL222453A0 (en) 2012-12-31
US20130102032A1 (en) 2013-04-25
AU2011246504A1 (en) 2012-11-22
CN105331659A (zh) 2016-02-17
WO2011134921A1 (en) 2011-11-03
JP2016052310A (ja) 2016-04-14
EP2563903A1 (en) 2013-03-06
CA2797140C (en) 2018-01-23
US8986957B2 (en) 2015-03-24
KR101848945B1 (ko) 2018-04-16
CA2797140A1 (en) 2011-11-03
BR112012027434B1 (pt) 2020-09-29
US9243224B2 (en) 2016-01-26
SG185037A1 (en) 2012-11-29
BR112012027434A2 (pt) 2015-09-22
JP2013524826A (ja) 2013-06-20
JP6113653B2 (ja) 2017-04-12
IL222453B (en) 2018-11-29
EP2563903B1 (en) 2019-08-21
KR20130098869A (ko) 2013-09-05
RU2563353C2 (ru) 2015-09-20
MX2012012525A (es) 2012-11-23
AU2011246504B2 (en) 2013-09-26

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