AU2011246504B2 - Improved cell culture medium - Google Patents

Improved cell culture medium Download PDF

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Publication number
AU2011246504B2
AU2011246504B2 AU2011246504A AU2011246504A AU2011246504B2 AU 2011246504 B2 AU2011246504 B2 AU 2011246504B2 AU 2011246504 A AU2011246504 A AU 2011246504A AU 2011246504 A AU2011246504 A AU 2011246504A AU 2011246504 B2 AU2011246504 B2 AU 2011246504B2
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AU
Australia
Prior art keywords
cell culture
production
medium
choline chloride
polypeptide
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AU2011246504A
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AU2011246504A1 (en
Inventor
Wolfgang Ernst Gustav Budach
Helene C. Chassin
Kerstin Dorsch
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Novartis AG
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Novartis AG
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Application filed by Novartis AG filed Critical Novartis AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/005—Protein-free medium
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0031—Serum-free culture media
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C12N2500/95—Protein-free medium and culture conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/99—Serum-free medium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AU2011246504A 2010-04-26 2011-04-25 Improved cell culture medium Active AU2011246504B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US32783710P 2010-04-26 2010-04-26
US61/327,837 2010-04-26
PCT/EP2011/056509 WO2011134921A1 (en) 2010-04-26 2011-04-25 Improved cell culture medium

Publications (2)

Publication Number Publication Date
AU2011246504A1 AU2011246504A1 (en) 2012-11-22
AU2011246504B2 true AU2011246504B2 (en) 2013-09-26

Family

ID=44276356

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2011246504A Active AU2011246504B2 (en) 2010-04-26 2011-04-25 Improved cell culture medium

Country Status (14)

Country Link
US (2) US8986957B2 (enExample)
EP (2) EP2563903B1 (enExample)
JP (2) JP6113653B2 (enExample)
KR (1) KR101848945B1 (enExample)
CN (2) CN102858953B (enExample)
AU (1) AU2011246504B2 (enExample)
BR (1) BR112012027434B1 (enExample)
CA (1) CA2797140C (enExample)
ES (1) ES2755907T3 (enExample)
IL (1) IL222453B (enExample)
MX (1) MX2012012525A (enExample)
RU (1) RU2563353C2 (enExample)
SG (1) SG185037A1 (enExample)
WO (1) WO2011134921A1 (enExample)

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WO2014054744A1 (ja) * 2012-10-03 2014-04-10 協和発酵キリン株式会社 培養液にアミノ酸を添加することによるポリペプチドの還元防止方法
JP2015531244A (ja) 2012-10-15 2015-11-02 ブリストル−マイヤーズ スクイブ カンパニーＢｒｉｓｔｏｌ−Ｍｙｅｒｓ Ｓｑｕｉｂｂ Ｃｏｍｐａｎｙ タンパク質を製造するための哺乳動物細胞培養方法
CA2943667C (en) * 2014-01-29 2022-11-08 Lg Life Sciences Ltd. Method for modulating galactosylation of recombinant protein through optimization of culture medium
WO2015155975A1 (ja) * 2014-04-07 2015-10-15 東洋製罐グループホールディングス株式会社 動物細胞用培養液、及び培養容器
ES2733298T3 (es) 2014-07-18 2019-11-28 Sandoz Ag Cuantificación de TNFR2:Fc plegado erróneamente
KR20240093910A (ko) 2015-04-01 2024-06-24 베링거 인겔하임 인터내셔날 게엠베하 세포 배양 배지
WO2016192830A1 (en) * 2015-05-29 2016-12-08 Merck Patent Gmbh Deep eutectic solvents and/or ionic liquids in cell culture media
DK3303554T3 (da) * 2015-05-29 2020-08-31 Merck Patent Gmbh Dybe eutektiske opløsningsmidler og/eller ioniske væsker som fodringsmedie
WO2016207721A1 (en) 2015-06-25 2016-12-29 University Of Limerick Mechanical device for generating combinatorial library
WO2017083224A1 (en) * 2015-11-09 2017-05-18 Bristol-Myers Squibb Company Methods to manipulate quality attributes of polypeptides produced in cho cells
KR20190005966A (ko) * 2016-05-10 2019-01-16 제넨테크, 인크. 폴리펩타이드의 재조합 생산 동안 트리설파이드 결합을 감소시키기 위한 방법
KR102893388B1 (ko) * 2016-10-31 2025-11-28 삼성바이오에피스 주식회사 세포 배양 배지에서 원치 않는 배양 부산물을 감소시키는 공정
CN106676058B (zh) * 2016-12-09 2020-06-19 中农威特生物科技股份有限公司 一种bhk21悬浮细胞高密度流加培养方法及其在口蹄疫病毒增殖中的应用
GB201708655D0 (en) * 2017-05-31 2017-07-12 Ucb Biopharma Sprl Cell culture methods
KR20220097421A (ko) 2019-11-05 2022-07-07 아지노모토 가부시키가이샤 단백질의 제조 방법
CA3167733A1 (en) * 2020-01-14 2021-07-22 Ajinomoto Co., Inc. High-density cell culture method

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US20090061516A1 (en) * 1999-04-30 2009-03-05 Invitrogen Corporation Animal cell culture media comprising non animal or plant derived nutrients

Also Published As

Publication number Publication date
AU2011246504A1 (en) 2012-11-22
JP6113653B2 (ja) 2017-04-12
CA2797140A1 (en) 2011-11-03
EP3599276A1 (en) 2020-01-29
SG185037A1 (en) 2012-11-29
US9243224B2 (en) 2016-01-26
JP2013524826A (ja) 2013-06-20
CA2797140C (en) 2018-01-23
EP2563903A1 (en) 2013-03-06
CN105331659B (zh) 2019-07-16
BR112012027434A2 (pt) 2015-09-22
KR101848945B1 (ko) 2018-04-16
WO2011134921A1 (en) 2011-11-03
US20130102032A1 (en) 2013-04-25
US8986957B2 (en) 2015-03-24
RU2012150281A (ru) 2014-06-10
CN102858953B (zh) 2015-09-09
JP2016052310A (ja) 2016-04-14
KR20130098869A (ko) 2013-09-05
CN105331659A (zh) 2016-02-17
US20150218509A1 (en) 2015-08-06
BR112012027434B1 (pt) 2020-09-29
RU2563353C2 (ru) 2015-09-20
IL222453B (en) 2018-11-29
IL222453A0 (en) 2012-12-31
EP2563903B1 (en) 2019-08-21
CN102858953A (zh) 2013-01-02
ES2755907T3 (es) 2020-04-24
MX2012012525A (es) 2012-11-23

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