AU2011246504B2 - Improved cell culture medium - Google Patents

Improved cell culture medium Download PDF

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Publication number
AU2011246504B2
AU2011246504B2 AU2011246504A AU2011246504A AU2011246504B2 AU 2011246504 B2 AU2011246504 B2 AU 2011246504B2 AU 2011246504 A AU2011246504 A AU 2011246504A AU 2011246504 A AU2011246504 A AU 2011246504A AU 2011246504 B2 AU2011246504 B2 AU 2011246504B2
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AU
Australia
Prior art keywords
cell culture
production
medium
choline chloride
polypeptide
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AU2011246504A
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English (en)
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AU2011246504A1 (en
Inventor
Wolfgang Ernst Gustav Budach
Helene C. Chassin
Kerstin Dorsch
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Novartis AG
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Novartis AG
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=44276356&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=AU2011246504(B2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Novartis AG filed Critical Novartis AG
Publication of AU2011246504A1 publication Critical patent/AU2011246504A1/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/005—Protein-free medium
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0031—Serum-free culture media
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C12N2500/95—Protein-free medium and culture conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/99—Serum-free medium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AU2011246504A 2010-04-26 2011-04-25 Improved cell culture medium Active AU2011246504B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US32783710P 2010-04-26 2010-04-26
US61/327,837 2010-04-26
PCT/EP2011/056509 WO2011134921A1 (en) 2010-04-26 2011-04-25 Improved cell culture medium

Publications (2)

Publication Number Publication Date
AU2011246504A1 AU2011246504A1 (en) 2012-11-22
AU2011246504B2 true AU2011246504B2 (en) 2013-09-26

Family

ID=44276356

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2011246504A Active AU2011246504B2 (en) 2010-04-26 2011-04-25 Improved cell culture medium

Country Status (14)

Country Link
US (2) US8986957B2 (enExample)
EP (2) EP3599276A1 (enExample)
JP (2) JP6113653B2 (enExample)
KR (1) KR101848945B1 (enExample)
CN (2) CN105331659B (enExample)
AU (1) AU2011246504B2 (enExample)
BR (1) BR112012027434B1 (enExample)
CA (1) CA2797140C (enExample)
ES (1) ES2755907T3 (enExample)
IL (1) IL222453B (enExample)
MX (1) MX2012012525A (enExample)
RU (1) RU2563353C2 (enExample)
SG (1) SG185037A1 (enExample)
WO (1) WO2011134921A1 (enExample)

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US9976163B2 (en) 2012-10-03 2018-05-22 Kyowa Hakko Kirin Co., Ltd Method for preventing reduction of polypeptide by adding amino acid to culture solution
CN104822826B (zh) 2012-10-15 2019-04-30 百时美施贵宝公司 用于蛋白产生的哺乳动物细胞培养过程
CA2943667C (en) * 2014-01-29 2022-11-08 Lg Life Sciences Ltd. Method for modulating galactosylation of recombinant protein through optimization of culture medium
WO2015155975A1 (ja) * 2014-04-07 2015-10-15 東洋製罐グループホールディングス株式会社 動物細胞用培養液、及び培養容器
ES2733298T3 (es) 2014-07-18 2019-11-28 Sandoz Ag Cuantificación de TNFR2:Fc plegado erróneamente
SG11201705743YA (en) * 2015-04-01 2017-08-30 Boehringer Ingelheim Int Cell culture medium
EP3303555B1 (en) * 2015-05-29 2020-06-24 Merck Patent GmbH Deep eutectic solvents and/or ionic liquids in cell culture media
EP3303554B1 (en) * 2015-05-29 2020-06-24 Merck Patent GmbH Deep eutectic solvents and/or ionic liquids as feed media
WO2016207721A1 (en) 2015-06-25 2016-12-29 University Of Limerick Mechanical device for generating combinatorial library
JP2018536404A (ja) * 2015-11-09 2018-12-13 ブリストル−マイヤーズ スクイブ カンパニーＢｒｉｓｔｏｌ−Ｍｙｅｒｓ Ｓｑｕｉｂｂ Ｃｏｍｐａｎｙ Ｃｈｏ細胞において産生したポリペプチドの品質特性を操作する方法
KR20230124093A (ko) * 2016-05-10 2023-08-24 제넨테크, 인크. 폴리펩타이드의 재조합 생산 동안 트리설파이드 결합을감소시키기 위한 방법
KR102893388B1 (ko) * 2016-10-31 2025-11-28 삼성바이오에피스 주식회사 세포 배양 배지에서 원치 않는 배양 부산물을 감소시키는 공정
CN106676058B (zh) * 2016-12-09 2020-06-19 中农威特生物科技股份有限公司 一种bhk21悬浮细胞高密度流加培养方法及其在口蹄疫病毒增殖中的应用
GB201708655D0 (en) * 2017-05-31 2017-07-12 Ucb Biopharma Sprl Cell culture methods
EP4056678A4 (en) * 2019-11-05 2023-12-06 Ajinomoto Co., Inc. Method for producing protein
CA3167733A1 (en) * 2020-01-14 2021-07-22 Ajinomoto Co., Inc. High-density cell culture method

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US6048728A (en) * 1988-09-23 2000-04-11 Chiron Corporation Cell culture medium for enhanced cell growth, culture longevity, and product expression
US20090061516A1 (en) * 1999-04-30 2009-03-05 Invitrogen Corporation Animal cell culture media comprising non animal or plant derived nutrients

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WO1992015670A1 (en) * 1991-02-28 1992-09-17 Hyclone Laboratories, Inc. Blended bovine sera cellular growth media and their methods of production and use
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US20090061516A1 (en) * 1999-04-30 2009-03-05 Invitrogen Corporation Animal cell culture media comprising non animal or plant derived nutrients

Also Published As

Publication number Publication date
RU2012150281A (ru) 2014-06-10
US20130102032A1 (en) 2013-04-25
IL222453B (en) 2018-11-29
JP6113653B2 (ja) 2017-04-12
BR112012027434A2 (pt) 2015-09-22
KR20130098869A (ko) 2013-09-05
CN102858953B (zh) 2015-09-09
CN105331659B (zh) 2019-07-16
ES2755907T3 (es) 2020-04-24
EP3599276A1 (en) 2020-01-29
US9243224B2 (en) 2016-01-26
KR101848945B1 (ko) 2018-04-16
EP2563903B1 (en) 2019-08-21
CA2797140C (en) 2018-01-23
IL222453A0 (en) 2012-12-31
JP2016052310A (ja) 2016-04-14
CA2797140A1 (en) 2011-11-03
AU2011246504A1 (en) 2012-11-22
MX2012012525A (es) 2012-11-23
US20150218509A1 (en) 2015-08-06
JP2013524826A (ja) 2013-06-20
CN102858953A (zh) 2013-01-02
US8986957B2 (en) 2015-03-24
BR112012027434B1 (pt) 2020-09-29
CN105331659A (zh) 2016-02-17
WO2011134921A1 (en) 2011-11-03
RU2563353C2 (ru) 2015-09-20
EP2563903A1 (en) 2013-03-06
SG185037A1 (en) 2012-11-29

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