JP2013517761A5 - - Google Patents
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- Publication number
- JP2013517761A5 JP2013517761A5 JP2012549441A JP2012549441A JP2013517761A5 JP 2013517761 A5 JP2013517761 A5 JP 2013517761A5 JP 2012549441 A JP2012549441 A JP 2012549441A JP 2012549441 A JP2012549441 A JP 2012549441A JP 2013517761 A5 JP2013517761 A5 JP 2013517761A5
- Authority
- JP
- Japan
- Prior art keywords
- cell
- antibody
- polypeptide
- antibody polypeptide
- yarrowia
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 210000004027 cell Anatomy 0.000 claims 142
- 108090000765 processed proteins & peptides Proteins 0.000 claims 97
- 102000004196 processed proteins & peptides Human genes 0.000 claims 96
- 229920001184 polypeptide Polymers 0.000 claims 95
- 238000000034 method Methods 0.000 claims 64
- 241000235013 Yarrowia Species 0.000 claims 49
- 239000012634 fragment Substances 0.000 claims 49
- 150000007523 nucleic acids Chemical group 0.000 claims 26
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 25
- 239000002773 nucleotide Substances 0.000 claims 25
- 125000003729 nucleotide group Chemical group 0.000 claims 25
- 239000013598 vector Substances 0.000 claims 18
- 230000004927 fusion Effects 0.000 claims 14
- 108091033319 polynucleotide Proteins 0.000 claims 12
- 102000040430 polynucleotide Human genes 0.000 claims 12
- 239000002157 polynucleotide Substances 0.000 claims 12
- 238000011534 incubation Methods 0.000 claims 8
- 239000003550 marker Substances 0.000 claims 8
- 101710112477 Phycobiliprotein ApcE Proteins 0.000 claims 7
- 230000006698 induction Effects 0.000 claims 6
- 230000010076 replication Effects 0.000 claims 6
- 102000001706 Immunoglobulin Fab Fragments Human genes 0.000 claims 5
- 108010054477 Immunoglobulin Fab Fragments Proteins 0.000 claims 5
- 210000004899 c-terminal region Anatomy 0.000 claims 4
- 210000002230 centromere Anatomy 0.000 claims 4
- 102100034613 Annexin A2 Human genes 0.000 claims 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims 3
- 101000924474 Homo sapiens Annexin A2 Proteins 0.000 claims 3
- 230000001939 inductive effect Effects 0.000 claims 3
- 239000002609 medium Substances 0.000 claims 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims 2
- 101710186708 Agglutinin Proteins 0.000 claims 2
- 101710146024 Horcolin Proteins 0.000 claims 2
- 101710189395 Lectin Proteins 0.000 claims 2
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 claims 2
- 101710179758 Mannose-specific lectin Proteins 0.000 claims 2
- 101710150763 Mannose-specific lectin 1 Proteins 0.000 claims 2
- 101710150745 Mannose-specific lectin 2 Proteins 0.000 claims 2
- 108010006519 Molecular Chaperones Proteins 0.000 claims 2
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 claims 2
- 101100215634 Yarrowia lipolytica (strain CLIB 122 / E 150) XPR2 gene Proteins 0.000 claims 2
- 238000005273 aeration Methods 0.000 claims 2
- 239000000910 agglutinin Substances 0.000 claims 2
- 229930024421 Adenine Natural products 0.000 claims 1
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 claims 1
- 239000004475 Arginine Substances 0.000 claims 1
- 108010023063 Bacto-peptone Proteins 0.000 claims 1
- 102100037620 Centrin-1 Human genes 0.000 claims 1
- 102100037633 Centrin-3 Human genes 0.000 claims 1
- 108020004705 Codon Proteins 0.000 claims 1
- 102000005720 Glutathione transferase Human genes 0.000 claims 1
- 108010070675 Glutathione transferase Proteins 0.000 claims 1
- BCCRXDTUTZHDEU-VKHMYHEASA-N Gly-Ser Chemical compound NCC(=O)N[C@@H](CO)C(O)=O BCCRXDTUTZHDEU-VKHMYHEASA-N 0.000 claims 1
- 101710154606 Hemagglutinin Proteins 0.000 claims 1
- 108010093488 His-His-His-His-His-His Proteins 0.000 claims 1
- 101000880499 Homo sapiens Centrin-1 Proteins 0.000 claims 1
- 101000880522 Homo sapiens Centrin-3 Proteins 0.000 claims 1
- 101100084403 Homo sapiens PRODH gene Proteins 0.000 claims 1
- GRRNUXAQVGOGFE-UHFFFAOYSA-N Hygromycin-B Natural products OC1C(NC)CC(N)C(O)C1OC1C2OC3(C(C(O)C(O)C(C(N)CO)O3)O)OC2C(O)C(CO)O1 GRRNUXAQVGOGFE-UHFFFAOYSA-N 0.000 claims 1
- 102000008394 Immunoglobulin Fragments Human genes 0.000 claims 1
- 108010021625 Immunoglobulin Fragments Proteins 0.000 claims 1
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 claims 1
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 claims 1
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 claims 1
- 239000004472 Lysine Substances 0.000 claims 1
- 102000005431 Molecular Chaperones Human genes 0.000 claims 1
- 102100038895 Myc proto-oncogene protein Human genes 0.000 claims 1
- 101710135898 Myc proto-oncogene protein Proteins 0.000 claims 1
- 101710093908 Outer capsid protein VP4 Proteins 0.000 claims 1
- 101710135467 Outer capsid protein sigma-1 Proteins 0.000 claims 1
- 101150059359 POX2 gene Proteins 0.000 claims 1
- 102000007079 Peptide Fragments Human genes 0.000 claims 1
- 108010033276 Peptide Fragments Proteins 0.000 claims 1
- 102100028772 Proline dehydrogenase 1, mitochondrial Human genes 0.000 claims 1
- 101710176177 Protein A56 Proteins 0.000 claims 1
- 102000006010 Protein Disulfide-Isomerase Human genes 0.000 claims 1
- 108010090804 Streptavidin Proteins 0.000 claims 1
- 101710150448 Transcriptional regulator Myc Proteins 0.000 claims 1
- 102000004357 Transferases Human genes 0.000 claims 1
- 108090000992 Transferases Proteins 0.000 claims 1
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 claims 1
- 101100029251 Zea mays PER2 gene Proteins 0.000 claims 1
- 229960000643 adenine Drugs 0.000 claims 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 claims 1
- 229960002685 biotin Drugs 0.000 claims 1
- 235000020958 biotin Nutrition 0.000 claims 1
- 239000011616 biotin Substances 0.000 claims 1
- 229940041514 candida albicans extract Drugs 0.000 claims 1
- 210000001840 diploid cell Anatomy 0.000 claims 1
- 108010033706 glycylserine Proteins 0.000 claims 1
- 239000001963 growth medium Substances 0.000 claims 1
- 210000003783 haploid cell Anatomy 0.000 claims 1
- 239000000185 hemagglutinin Substances 0.000 claims 1
- -1 hygromycin B phospho Chemical class 0.000 claims 1
- 229940097277 hygromycin b Drugs 0.000 claims 1
- 102000028557 immunoglobulin binding proteins Human genes 0.000 claims 1
- 108091009323 immunoglobulin binding proteins Proteins 0.000 claims 1
- 230000013011 mating Effects 0.000 claims 1
- 108020003519 protein disulfide isomerase Proteins 0.000 claims 1
- 102220080600 rs797046116 Human genes 0.000 claims 1
- AWUCVROLDVIAJX-GSVOUGTGSA-N sn-glycerol 3-phosphate Chemical compound OC[C@@H](O)COP(O)(O)=O AWUCVROLDVIAJX-GSVOUGTGSA-N 0.000 claims 1
- 229940035893 uracil Drugs 0.000 claims 1
- 239000012138 yeast extract Substances 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US29709310P | 2010-01-21 | 2010-01-21 | |
| US61/297,093 | 2010-01-21 | ||
| PCT/IB2011/000227 WO2011089527A1 (en) | 2010-01-21 | 2011-01-21 | Methods and compositions for displaying a poypeptide on a yeast cell surface |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2013517761A JP2013517761A (ja) | 2013-05-20 |
| JP2013517761A5 true JP2013517761A5 (enExample) | 2014-03-13 |
Family
ID=44022829
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2012549441A Pending JP2013517761A (ja) | 2010-01-21 | 2011-01-21 | 酵母細胞の表面上にポリペプチドをディスプレイするための方法および組成物 |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US20130096281A1 (enExample) |
| EP (1) | EP2526193A1 (enExample) |
| JP (1) | JP2013517761A (enExample) |
| KR (1) | KR20120118045A (enExample) |
| CN (1) | CN102803491A (enExample) |
| BR (1) | BR112012018163A2 (enExample) |
| CA (1) | CA2787677A1 (enExample) |
| SG (1) | SG182647A1 (enExample) |
| WO (1) | WO2011089527A1 (enExample) |
Families Citing this family (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2508613A3 (en) | 2007-04-03 | 2012-11-28 | Oxyrane UK Limited | Glycosylation of molecules |
| JP5990102B2 (ja) | 2009-09-29 | 2016-09-07 | ユニフェルシテイト ヘント | ホスホ−６−マンノースへのマンノース−１−ホスホ−６−マンノース結合の加水分解 |
| BR112012011980A2 (pt) | 2009-11-19 | 2021-09-08 | Oxyrane Uk Limited | Métodos de produzir células transformadas de yarrowia lipolytica e proteínas-alvocompreendendo n-glicanos, células transformadas de yarrowia lipolytica e suas culturas, bem como composição compreendendo glicoproteínas |
| SG189110A1 (en) | 2010-09-29 | 2013-05-31 | Oxyrane Uk Ltd | De-mannosylation of phosphorylated n-glycans |
| CA2812870C (en) | 2010-09-29 | 2020-06-09 | Oxyrane Uk Limited | Mannosidases capable of uncapping mannose-1-phospho-6-mannose linkages and demannosylating phosphorylated n-glycans and methods of facilitating mammalian cellular uptake of glycoproteins |
| US20140243234A1 (en) * | 2011-08-03 | 2014-08-28 | Texas Biomedical Research Institute | Nucleic acid compositions, methods and kits for rapid pairing of affinity agents |
| CN104379162B (zh) | 2012-03-15 | 2017-03-15 | 奥克西雷恩英国有限公司 | 用于治疗蓬佩氏病的方法和材料 |
| KR102150414B1 (ko) * | 2013-09-18 | 2020-09-02 | 리제너론 파마슈티칼스 인코포레이티드 | 히스티딘 엔지니어링된 경쇄 항체 및 그것을 생성하기 위한 유전자 변형된 비-인간 동물 |
| HRP20201906T1 (hr) | 2013-12-20 | 2021-04-02 | Fred Hutchinson Cancer Research Center | Označene kimerne efektorske molekule i njihovi receptori |
| WO2016075314A1 (fr) | 2014-11-13 | 2016-05-19 | Institut National De La Recherche Agronomique | Identification de facteurs de transcription de yarrowia lipolytica affectant la production de proteines |
| FR3028527A1 (fr) | 2014-11-13 | 2016-05-20 | Pivert | Identification de facteurs de transcription de yarrowia lipolytica |
| US20160185633A1 (en) * | 2014-12-30 | 2016-06-30 | University Of Florida Research Foundation, Inc. | Recovery of nutrients from water and wastewater by precipitation as struvite |
| WO2016176652A2 (en) | 2015-04-29 | 2016-11-03 | Fred Hutchinson Cancer Research Center | Modified stem cells and uses thereof |
| EA201891521A1 (ru) * | 2016-01-08 | 2019-01-31 | ДСМ АйПи АССЕТС Б.В. | Переключение типа спаривания у yarrowia lipolytica |
| US10988759B2 (en) | 2016-01-15 | 2021-04-27 | University Of Washington | High throughput protein-protein interaction screening in yeast liquid culture |
| CN106701606B (zh) * | 2016-12-19 | 2021-03-26 | 广东利世康低碳科技有限公司 | 一种能降解利用餐厨废弃物的基因工程产朊假丝酵母及其构建方法 |
| CN108085332B (zh) * | 2017-12-27 | 2020-11-27 | 中国科学院青岛生物能源与过程研究所 | 一种细胞表面展示有豆壳过氧化物酶的重组酵母菌及其构建方法与应用 |
| FR3081169B1 (fr) | 2018-05-15 | 2020-06-19 | Messenger Biopharma | Substitution de la coiffe des arn messagers par deux sequences d'arn introduites a leur extremite 5' |
| WO2021231013A1 (en) * | 2020-05-11 | 2021-11-18 | A-Alpha Bio, Inc. | High-throughput screening methods to identify small molecule targets |
| EP4158015A1 (en) | 2020-06-01 | 2023-04-05 | A-Alpha Bio, Inc. | Methods for characterizing and engineering protein-protein interactions |
| CN111996132A (zh) * | 2020-07-21 | 2020-11-27 | 郑州大学 | 一种解脂耶氏酵母表面展示方法 |
| WO2023051972A1 (en) | 2021-09-29 | 2023-04-06 | Miltenyi Biotec B.V. & Co. KG | Method for the generation and selection of a producer cell |
| CN114606151B (zh) * | 2022-04-27 | 2023-05-19 | 南京工业大学 | 一种表面展示β-半乳糖苷酶的重组毕赤酵母及构建方法和应用 |
| CN115851460A (zh) * | 2022-08-10 | 2023-03-28 | 丁辰 | 一种具有激活哺乳动物干细胞分化功能的菌株、组合物及其应用 |
| CN115786387B (zh) * | 2022-09-19 | 2024-02-13 | 苏州泓迅生物科技股份有限公司 | 一种通用型酵母细胞表面展示质粒载体及其应用 |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5530101A (en) | 1988-12-28 | 1996-06-25 | Protein Design Labs, Inc. | Humanized immunoglobulins |
| US6042828A (en) | 1992-09-07 | 2000-03-28 | Kyowa Hakko Kogyo Co., Ltd. | Humanized antibodies to ganglioside GM2 |
| US6699658B1 (en) * | 1996-05-31 | 2004-03-02 | Board Of Trustees Of The University Of Illinois | Yeast cell surface display of proteins and uses thereof |
| US6300065B1 (en) * | 1996-05-31 | 2001-10-09 | Board Of Trustees Of The University Of Illinois | Yeast cell surface display of proteins and uses thereof |
| US7442772B2 (en) | 1997-12-03 | 2008-10-28 | Genentech, Inc. | Antibodies to PRO361 polypeptide |
| WO2001042462A2 (en) | 1999-12-08 | 2001-06-14 | National University Of Singapore | Phospholipase a2 inhibitory peptides from python reticulatus |
| ES2405551T3 (es) * | 2001-10-01 | 2013-05-31 | Dyax Corporation | Vectores de presentación eucariotas multicatenarios y usos de los mismos |
| MXPA04009418A (es) | 2002-03-29 | 2005-06-08 | Schering Corp | Anticuerpos monoclonales humanos par interleucina-5, y metodos y composiciones que comprenden los mismos. |
| CA2501422C (en) | 2004-04-29 | 2014-08-12 | University Of Rochester | Lymphoid chemokines in the diagnosis, monitoring and treatment of autoimmune disease |
| US7431927B2 (en) | 2005-03-24 | 2008-10-07 | Epitomics, Inc. | TNFα-neutralizing antibodies |
| CA2604917A1 (en) * | 2005-04-14 | 2007-01-25 | Csir | Recombinant yeasts for synthesizing epoxide hydrolases |
| WO2008100816A2 (en) * | 2007-02-09 | 2008-08-21 | Medimmune, Llc | Antibody libray display by yeast cell plasma membrane |
-
2011
- 2011-01-21 WO PCT/IB2011/000227 patent/WO2011089527A1/en not_active Ceased
- 2011-01-21 KR KR1020127021903A patent/KR20120118045A/ko not_active Withdrawn
- 2011-01-21 BR BR112012018163A patent/BR112012018163A2/pt not_active IP Right Cessation
- 2011-01-21 CN CN2011800148893A patent/CN102803491A/zh active Pending
- 2011-01-21 EP EP11710288A patent/EP2526193A1/en not_active Withdrawn
- 2011-01-21 US US13/574,126 patent/US20130096281A1/en not_active Abandoned
- 2011-01-21 SG SG2012053948A patent/SG182647A1/en unknown
- 2011-01-21 CA CA2787677A patent/CA2787677A1/en not_active Abandoned
- 2011-01-21 JP JP2012549441A patent/JP2013517761A/ja active Pending
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