BR112012018163A2 - métodos e composições para exibição de um polipeptídeo sobre a superfície de uma célula de levredura - Google Patents
métodos e composições para exibição de um polipeptídeo sobre a superfície de uma célula de levreduraInfo
- Publication number
- BR112012018163A2 BR112012018163A2 BR112012018163A BR112012018163A BR112012018163A2 BR 112012018163 A2 BR112012018163 A2 BR 112012018163A2 BR 112012018163 A BR112012018163 A BR 112012018163A BR 112012018163 A BR112012018163 A BR 112012018163A BR 112012018163 A2 BR112012018163 A2 BR 112012018163A2
- Authority
- BR
- Brazil
- Prior art keywords
- polypeptide
- compositions
- methods
- displaying
- yeast cell
- Prior art date
Links
- 229920001184 polypeptide Polymers 0.000 title abstract 5
- 108090000765 processed proteins & peptides Proteins 0.000 title abstract 5
- 102000004196 processed proteins & peptides Human genes 0.000 title abstract 5
- 239000000203 mixture Substances 0.000 title abstract 4
- 210000005253 yeast cell Anatomy 0.000 title abstract 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 abstract 1
- 241000235013 Yarrowia Species 0.000 abstract 1
- 241000235015 Yarrowia lipolytica Species 0.000 abstract 1
- 239000012634 fragment Substances 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/32—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
- C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
- C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/55—Fab or Fab'
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
- C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
- C07K2317/622—Single chain antibody (scFv)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/035—Fusion polypeptide containing a localisation/targetting motif containing a signal for targeting to the external surface of a cell, e.g. to the outer membrane of Gram negative bacteria, GPI- anchored eukaryote proteins
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Virology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Oncology (AREA)
- Botany (AREA)
- Tropical Medicine & Parasitology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US29709310P | 2010-01-21 | 2010-01-21 | |
| PCT/IB2011/000227 WO2011089527A1 (en) | 2010-01-21 | 2011-01-21 | Methods and compositions for displaying a poypeptide on a yeast cell surface |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR112012018163A2 true BR112012018163A2 (pt) | 2019-09-24 |
Family
ID=44022829
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112012018163A BR112012018163A2 (pt) | 2010-01-21 | 2011-01-21 | métodos e composições para exibição de um polipeptídeo sobre a superfície de uma célula de levredura |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US20130096281A1 (enExample) |
| EP (1) | EP2526193A1 (enExample) |
| JP (1) | JP2013517761A (enExample) |
| KR (1) | KR20120118045A (enExample) |
| CN (1) | CN102803491A (enExample) |
| BR (1) | BR112012018163A2 (enExample) |
| CA (1) | CA2787677A1 (enExample) |
| SG (1) | SG182647A1 (enExample) |
| WO (1) | WO2011089527A1 (enExample) |
Families Citing this family (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2508613A3 (en) | 2007-04-03 | 2012-11-28 | Oxyrane UK Limited | Glycosylation of molecules |
| JP5990102B2 (ja) | 2009-09-29 | 2016-09-07 | ユニフェルシテイト ヘント | ホスホ−６−マンノースへのマンノース−１−ホスホ−６−マンノース結合の加水分解 |
| BR112012011980A2 (pt) | 2009-11-19 | 2021-09-08 | Oxyrane Uk Limited | Métodos de produzir células transformadas de yarrowia lipolytica e proteínas-alvocompreendendo n-glicanos, células transformadas de yarrowia lipolytica e suas culturas, bem como composição compreendendo glicoproteínas |
| SG189110A1 (en) | 2010-09-29 | 2013-05-31 | Oxyrane Uk Ltd | De-mannosylation of phosphorylated n-glycans |
| CA2812870C (en) | 2010-09-29 | 2020-06-09 | Oxyrane Uk Limited | Mannosidases capable of uncapping mannose-1-phospho-6-mannose linkages and demannosylating phosphorylated n-glycans and methods of facilitating mammalian cellular uptake of glycoproteins |
| US20140243234A1 (en) * | 2011-08-03 | 2014-08-28 | Texas Biomedical Research Institute | Nucleic acid compositions, methods and kits for rapid pairing of affinity agents |
| CN104379162B (zh) | 2012-03-15 | 2017-03-15 | 奥克西雷恩英国有限公司 | 用于治疗蓬佩氏病的方法和材料 |
| KR102150414B1 (ko) * | 2013-09-18 | 2020-09-02 | 리제너론 파마슈티칼스 인코포레이티드 | 히스티딘 엔지니어링된 경쇄 항체 및 그것을 생성하기 위한 유전자 변형된 비-인간 동물 |
| HRP20201906T1 (hr) | 2013-12-20 | 2021-04-02 | Fred Hutchinson Cancer Research Center | Označene kimerne efektorske molekule i njihovi receptori |
| WO2016075314A1 (fr) | 2014-11-13 | 2016-05-19 | Institut National De La Recherche Agronomique | Identification de facteurs de transcription de yarrowia lipolytica affectant la production de proteines |
| FR3028527A1 (fr) | 2014-11-13 | 2016-05-20 | Pivert | Identification de facteurs de transcription de yarrowia lipolytica |
| US20160185633A1 (en) * | 2014-12-30 | 2016-06-30 | University Of Florida Research Foundation, Inc. | Recovery of nutrients from water and wastewater by precipitation as struvite |
| WO2016176652A2 (en) | 2015-04-29 | 2016-11-03 | Fred Hutchinson Cancer Research Center | Modified stem cells and uses thereof |
| EA201891521A1 (ru) * | 2016-01-08 | 2019-01-31 | ДСМ АйПи АССЕТС Б.В. | Переключение типа спаривания у yarrowia lipolytica |
| US10988759B2 (en) | 2016-01-15 | 2021-04-27 | University Of Washington | High throughput protein-protein interaction screening in yeast liquid culture |
| CN106701606B (zh) * | 2016-12-19 | 2021-03-26 | 广东利世康低碳科技有限公司 | 一种能降解利用餐厨废弃物的基因工程产朊假丝酵母及其构建方法 |
| CN108085332B (zh) * | 2017-12-27 | 2020-11-27 | 中国科学院青岛生物能源与过程研究所 | 一种细胞表面展示有豆壳过氧化物酶的重组酵母菌及其构建方法与应用 |
| FR3081169B1 (fr) | 2018-05-15 | 2020-06-19 | Messenger Biopharma | Substitution de la coiffe des arn messagers par deux sequences d'arn introduites a leur extremite 5' |
| WO2021231013A1 (en) * | 2020-05-11 | 2021-11-18 | A-Alpha Bio, Inc. | High-throughput screening methods to identify small molecule targets |
| EP4158015A1 (en) | 2020-06-01 | 2023-04-05 | A-Alpha Bio, Inc. | Methods for characterizing and engineering protein-protein interactions |
| CN111996132A (zh) * | 2020-07-21 | 2020-11-27 | 郑州大学 | 一种解脂耶氏酵母表面展示方法 |
| WO2023051972A1 (en) | 2021-09-29 | 2023-04-06 | Miltenyi Biotec B.V. & Co. KG | Method for the generation and selection of a producer cell |
| CN114606151B (zh) * | 2022-04-27 | 2023-05-19 | 南京工业大学 | 一种表面展示β-半乳糖苷酶的重组毕赤酵母及构建方法和应用 |
| CN115851460A (zh) * | 2022-08-10 | 2023-03-28 | 丁辰 | 一种具有激活哺乳动物干细胞分化功能的菌株、组合物及其应用 |
| CN115786387B (zh) * | 2022-09-19 | 2024-02-13 | 苏州泓迅生物科技股份有限公司 | 一种通用型酵母细胞表面展示质粒载体及其应用 |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5530101A (en) | 1988-12-28 | 1996-06-25 | Protein Design Labs, Inc. | Humanized immunoglobulins |
| US6042828A (en) | 1992-09-07 | 2000-03-28 | Kyowa Hakko Kogyo Co., Ltd. | Humanized antibodies to ganglioside GM2 |
| US6699658B1 (en) * | 1996-05-31 | 2004-03-02 | Board Of Trustees Of The University Of Illinois | Yeast cell surface display of proteins and uses thereof |
| US6300065B1 (en) * | 1996-05-31 | 2001-10-09 | Board Of Trustees Of The University Of Illinois | Yeast cell surface display of proteins and uses thereof |
| US7442772B2 (en) | 1997-12-03 | 2008-10-28 | Genentech, Inc. | Antibodies to PRO361 polypeptide |
| WO2001042462A2 (en) | 1999-12-08 | 2001-06-14 | National University Of Singapore | Phospholipase a2 inhibitory peptides from python reticulatus |
| ES2405551T3 (es) * | 2001-10-01 | 2013-05-31 | Dyax Corporation | Vectores de presentación eucariotas multicatenarios y usos de los mismos |
| MXPA04009418A (es) | 2002-03-29 | 2005-06-08 | Schering Corp | Anticuerpos monoclonales humanos par interleucina-5, y metodos y composiciones que comprenden los mismos. |
| CA2501422C (en) | 2004-04-29 | 2014-08-12 | University Of Rochester | Lymphoid chemokines in the diagnosis, monitoring and treatment of autoimmune disease |
| US7431927B2 (en) | 2005-03-24 | 2008-10-07 | Epitomics, Inc. | TNFα-neutralizing antibodies |
| CA2604917A1 (en) * | 2005-04-14 | 2007-01-25 | Csir | Recombinant yeasts for synthesizing epoxide hydrolases |
| WO2008100816A2 (en) * | 2007-02-09 | 2008-08-21 | Medimmune, Llc | Antibody libray display by yeast cell plasma membrane |
-
2011
- 2011-01-21 WO PCT/IB2011/000227 patent/WO2011089527A1/en not_active Ceased
- 2011-01-21 KR KR1020127021903A patent/KR20120118045A/ko not_active Withdrawn
- 2011-01-21 BR BR112012018163A patent/BR112012018163A2/pt not_active IP Right Cessation
- 2011-01-21 CN CN2011800148893A patent/CN102803491A/zh active Pending
- 2011-01-21 EP EP11710288A patent/EP2526193A1/en not_active Withdrawn
- 2011-01-21 US US13/574,126 patent/US20130096281A1/en not_active Abandoned
- 2011-01-21 SG SG2012053948A patent/SG182647A1/en unknown
- 2011-01-21 CA CA2787677A patent/CA2787677A1/en not_active Abandoned
- 2011-01-21 JP JP2012549441A patent/JP2013517761A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| WO2011089527A1 (en) | 2011-07-28 |
| CN102803491A (zh) | 2012-11-28 |
| CA2787677A1 (en) | 2011-07-28 |
| JP2013517761A (ja) | 2013-05-20 |
| SG182647A1 (en) | 2012-08-30 |
| EP2526193A1 (en) | 2012-11-28 |
| KR20120118045A (ko) | 2012-10-25 |
| US20130096281A1 (en) | 2013-04-18 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B08F | Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette] | ||
| B08K | Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette] |
Free format text: EM VIRTUDE DO ARQUIVAMENTO PUBLICADO NA RPI 2543 DE 01-10-2019 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDO O ARQUIVAMENTO DO PEDIDO DE PATENTE, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013. |