CN102803491A - 用于在酵母细胞表面上展示多肽的方法和组合物 - Google Patents

用于在酵母细胞表面上展示多肽的方法和组合物 Download PDF

Info

Publication number
CN102803491A
CN102803491A CN2011800148893A CN201180014889A CN102803491A CN 102803491 A CN102803491 A CN 102803491A CN 2011800148893 A CN2011800148893 A CN 2011800148893A CN 201180014889 A CN201180014889 A CN 201180014889A CN 102803491 A CN102803491 A CN 102803491A
Authority
CN
China
Prior art keywords
polypeptide
nucleotide sequence
antibody polypeptides
cell
fragment
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN2011800148893A
Other languages
English (en)
Chinese (zh)
Inventor
S.里卡尔特
G.莱隆德尔
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Oxyrane UK Ltd
Original Assignee
Oxyrane UK Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Oxyrane UK Ltd filed Critical Oxyrane UK Ltd
Publication of CN102803491A publication Critical patent/CN102803491A/zh
Pending legal-status Critical Current

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/32Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • C12N1/16Yeasts; Culture media therefor
    • C12N1/18Baker's yeast; Brewer's yeast
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1037Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62DNA sequences coding for fusion proteins
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/10Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14Specific host cells or culture conditions, e.g. components, pH or temperature
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/20Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/50Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55Fab or Fab'
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/60Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622Single chain antibody (scFv)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/01Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/035Fusion polypeptide containing a localisation/targetting motif containing a signal for targeting to the external surface of a cell, e.g. to the outer membrane of Gram negative bacteria, GPI- anchored eukaryote proteins

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Oncology (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN2011800148893A 2010-01-21 2011-01-21 用于在酵母细胞表面上展示多肽的方法和组合物 Pending CN102803491A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US29709310P 2010-01-21 2010-01-21
US61/297,093 2010-01-21
PCT/IB2011/000227 WO2011089527A1 (en) 2010-01-21 2011-01-21 Methods and compositions for displaying a poypeptide on a yeast cell surface

Publications (1)

Publication Number Publication Date
CN102803491A true CN102803491A (zh) 2012-11-28

Family

ID=44022829

Family Applications (1)

Application Number Title Priority Date Filing Date
CN2011800148893A Pending CN102803491A (zh) 2010-01-21 2011-01-21 用于在酵母细胞表面上展示多肽的方法和组合物

Country Status (9)

Country Link
US (1) US20130096281A1 (enExample)
EP (1) EP2526193A1 (enExample)
JP (1) JP2013517761A (enExample)
KR (1) KR20120118045A (enExample)
CN (1) CN102803491A (enExample)
BR (1) BR112012018163A2 (enExample)
CA (1) CA2787677A1 (enExample)
SG (1) SG182647A1 (enExample)
WO (1) WO2011089527A1 (enExample)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105722387A (zh) * 2013-09-18 2016-06-29 瑞泽恩制药公司 组氨酸工程改造的轻链抗体和用于生成所述抗体的经基因修饰的非人动物
CN106701606A (zh) * 2016-12-19 2017-05-24 广东启智生物科技有限公司 一种能降解利用餐厨废弃物的基因工程产朊假丝酵母及其构建方法
CN108085332A (zh) * 2017-12-27 2018-05-29 中国科学院青岛生物能源与过程研究所 一种细胞表面展示有豆壳过氧化物酶的重组酵母菌及其构建方法与应用
CN111996132A (zh) * 2020-07-21 2020-11-27 郑州大学 一种解脂耶氏酵母表面展示方法
CN114606151A (zh) * 2022-04-27 2022-06-10 南京工业大学 一种表面展示β-半乳糖苷酶的重组毕赤酵母及构建方法和应用
CN115851460A (zh) * 2022-08-10 2023-03-28 丁辰 一种具有激活哺乳动物干细胞分化功能的菌株、组合物及其应用

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2508613A3 (en) 2007-04-03 2012-11-28 Oxyrane UK Limited Glycosylation of molecules
JP5990102B2 (ja) 2009-09-29 2016-09-07 ユニフェルシテイト ヘント ホスホ−6−マンノースへのマンノース−1−ホスホ−6−マンノース結合の加水分解
BR112012011980A2 (pt) 2009-11-19 2021-09-08 Oxyrane Uk Limited Métodos de produzir células transformadas de yarrowia lipolytica e proteínas-alvocompreendendo n-glicanos, células transformadas de yarrowia lipolytica e suas culturas, bem como composição compreendendo glicoproteínas
SG189110A1 (en) 2010-09-29 2013-05-31 Oxyrane Uk Ltd De-mannosylation of phosphorylated n-glycans
CA2812870C (en) 2010-09-29 2020-06-09 Oxyrane Uk Limited Mannosidases capable of uncapping mannose-1-phospho-6-mannose linkages and demannosylating phosphorylated n-glycans and methods of facilitating mammalian cellular uptake of glycoproteins
US20140243234A1 (en) * 2011-08-03 2014-08-28 Texas Biomedical Research Institute Nucleic acid compositions, methods and kits for rapid pairing of affinity agents
CN104379162B (zh) 2012-03-15 2017-03-15 奥克西雷恩英国有限公司 用于治疗蓬佩氏病的方法和材料
HRP20201906T1 (hr) 2013-12-20 2021-04-02 Fred Hutchinson Cancer Research Center Označene kimerne efektorske molekule i njihovi receptori
WO2016075314A1 (fr) 2014-11-13 2016-05-19 Institut National De La Recherche Agronomique Identification de facteurs de transcription de yarrowia lipolytica affectant la production de proteines
FR3028527A1 (fr) 2014-11-13 2016-05-20 Pivert Identification de facteurs de transcription de yarrowia lipolytica
US20160185633A1 (en) * 2014-12-30 2016-06-30 University Of Florida Research Foundation, Inc. Recovery of nutrients from water and wastewater by precipitation as struvite
WO2016176652A2 (en) 2015-04-29 2016-11-03 Fred Hutchinson Cancer Research Center Modified stem cells and uses thereof
EA201891521A1 (ru) * 2016-01-08 2019-01-31 ДСМ АйПи АССЕТС Б.В. Переключение типа спаривания у yarrowia lipolytica
US10988759B2 (en) 2016-01-15 2021-04-27 University Of Washington High throughput protein-protein interaction screening in yeast liquid culture
FR3081169B1 (fr) 2018-05-15 2020-06-19 Messenger Biopharma Substitution de la coiffe des arn messagers par deux sequences d'arn introduites a leur extremite 5'
WO2021231013A1 (en) * 2020-05-11 2021-11-18 A-Alpha Bio, Inc. High-throughput screening methods to identify small molecule targets
EP4158015A1 (en) 2020-06-01 2023-04-05 A-Alpha Bio, Inc. Methods for characterizing and engineering protein-protein interactions
WO2023051972A1 (en) 2021-09-29 2023-04-06 Miltenyi Biotec B.V. & Co. KG Method for the generation and selection of a producer cell
CN115786387B (zh) * 2022-09-19 2024-02-13 苏州泓迅生物科技股份有限公司 一种通用型酵母细胞表面展示质粒载体及其应用

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999036569A1 (en) * 1998-01-20 1999-07-22 The Board Of Trustees Of The University Of Illinois Yeast cell surface display of proteins and uses thereof
WO2003029456A1 (en) * 2001-10-01 2003-04-10 Dyax Corp. Multi-chain eukaryotic display vectors and uses thereof
US20080171359A1 (en) * 2005-04-14 2008-07-17 Oxyrane (Uk) Ltd. Recombinant Yeasts for Synthesizing Epoxide Hydrolases
WO2008100816A2 (en) * 2007-02-09 2008-08-21 Medimmune, Llc Antibody libray display by yeast cell plasma membrane

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5530101A (en) 1988-12-28 1996-06-25 Protein Design Labs, Inc. Humanized immunoglobulins
US6042828A (en) 1992-09-07 2000-03-28 Kyowa Hakko Kogyo Co., Ltd. Humanized antibodies to ganglioside GM2
US6699658B1 (en) * 1996-05-31 2004-03-02 Board Of Trustees Of The University Of Illinois Yeast cell surface display of proteins and uses thereof
US7442772B2 (en) 1997-12-03 2008-10-28 Genentech, Inc. Antibodies to PRO361 polypeptide
WO2001042462A2 (en) 1999-12-08 2001-06-14 National University Of Singapore Phospholipase a2 inhibitory peptides from python reticulatus
MXPA04009418A (es) 2002-03-29 2005-06-08 Schering Corp Anticuerpos monoclonales humanos par interleucina-5, y metodos y composiciones que comprenden los mismos.
CA2501422C (en) 2004-04-29 2014-08-12 University Of Rochester Lymphoid chemokines in the diagnosis, monitoring and treatment of autoimmune disease
US7431927B2 (en) 2005-03-24 2008-10-07 Epitomics, Inc. TNFα-neutralizing antibodies

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999036569A1 (en) * 1998-01-20 1999-07-22 The Board Of Trustees Of The University Of Illinois Yeast cell surface display of proteins and uses thereof
WO2003029456A1 (en) * 2001-10-01 2003-04-10 Dyax Corp. Multi-chain eukaryotic display vectors and uses thereof
US20080171359A1 (en) * 2005-04-14 2008-07-17 Oxyrane (Uk) Ltd. Recombinant Yeasts for Synthesizing Epoxide Hydrolases
WO2008100816A2 (en) * 2007-02-09 2008-08-21 Medimmune, Llc Antibody libray display by yeast cell plasma membrane

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BODER ET AL.: "Yeast surface dispaly for screening combination polypeptide libraries", 《NATURE BIOTECHNOLOGY》, vol. 15, 30 June 1997 (1997-06-30), pages 553 - 557 *
LIXI YUE ET AL.: "Construction of a new plasmid for surface display oncells of Yarrowia lipolytica", 《JOURNAL OF MICROBIOLOGICAL METHODS》, vol. 72, no. 2, 29 February 2008 (2008-02-29), pages 116 - 123, XP022420000 *

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105722387A (zh) * 2013-09-18 2016-06-29 瑞泽恩制药公司 组氨酸工程改造的轻链抗体和用于生成所述抗体的经基因修饰的非人动物
CN105722387B (zh) * 2013-09-18 2020-01-17 瑞泽恩制药公司 组氨酸工程改造的轻链抗体和用于生成所述抗体的经基因修饰的非人动物
CN106701606A (zh) * 2016-12-19 2017-05-24 广东启智生物科技有限公司 一种能降解利用餐厨废弃物的基因工程产朊假丝酵母及其构建方法
CN108085332A (zh) * 2017-12-27 2018-05-29 中国科学院青岛生物能源与过程研究所 一种细胞表面展示有豆壳过氧化物酶的重组酵母菌及其构建方法与应用
CN108085332B (zh) * 2017-12-27 2020-11-27 中国科学院青岛生物能源与过程研究所 一种细胞表面展示有豆壳过氧化物酶的重组酵母菌及其构建方法与应用
CN111996132A (zh) * 2020-07-21 2020-11-27 郑州大学 一种解脂耶氏酵母表面展示方法
CN114606151A (zh) * 2022-04-27 2022-06-10 南京工业大学 一种表面展示β-半乳糖苷酶的重组毕赤酵母及构建方法和应用
CN114606151B (zh) * 2022-04-27 2023-05-19 南京工业大学 一种表面展示β-半乳糖苷酶的重组毕赤酵母及构建方法和应用
CN115851460A (zh) * 2022-08-10 2023-03-28 丁辰 一种具有激活哺乳动物干细胞分化功能的菌株、组合物及其应用

Also Published As

Publication number Publication date
WO2011089527A1 (en) 2011-07-28
CA2787677A1 (en) 2011-07-28
JP2013517761A (ja) 2013-05-20
SG182647A1 (en) 2012-08-30
EP2526193A1 (en) 2012-11-28
BR112012018163A2 (pt) 2019-09-24
KR20120118045A (ko) 2012-10-25
US20130096281A1 (en) 2013-04-18

Similar Documents

Publication Publication Date Title
CN102803491A (zh) 用于在酵母细胞表面上展示多肽的方法和组合物
Rakestraw et al. Directed evolution of a secretory leader for the improved expression of heterologous proteins and full‐length antibodies in Saccharomyces cerevisiae
CN1871359B (zh) 使用单倍体交配策略在酵母中合成异聚多亚基多肽的方法
US5024941A (en) Expression and secretion vector for yeast containing a glucoamylase signal sequence
Oda et al. Identification of a cytoplasm to vacuole targeting determinant in aminopeptidase I.
WO2004020404A2 (en) Modified transferin-antibody fusion proteins
CN110055248A (zh) 可调控启动子
CN102268094A (zh) 被修饰的转铁蛋白抗体的融合蛋白
WO2005070962A1 (en) Production of a monoclonal antibody in a heterokaryon fungus or in a fungal host cell
JP2021505675A (ja) ペリプラズム空間中におけるタンパク質の酵母ディスプレイ
Lin et al. Dual N-and C-terminal helices are required for endoplasmic reticulum and lipid droplet association of alcohol acetyltransferases in Saccharomyces cerevisiae
Fukunishi et al. The fission yeast spore is coated by a proteinaceous surface layer comprising mainly Isp3
CN104531734A (zh) 改善的细菌膜蛋白分泌
TW201313899A (zh) 在經轉形的微生物如巴斯德畢赤氏酵母中的多-複製策略用於多-次級單元的蛋白質如抗體之高效價且高純度生產技術
Liu et al. Targeted introduction of a diphtheria toxin resistant mutation into the chromosomal EF-2 locus of Pichia pastoris and expression of immunotoxin in the EF-2 mutants
CN102741403A (zh) 二硫键连接的二聚体蛋白质在丝状噬菌体上的展示
Bleve et al. Subcellular localization and functional expression of the glycerol uptake protein 1 (GUP1) of Saccharomyces cerevisiae tagged with green fluorescent protein
EP2990485A1 (en) Fd chain gene or l chain gene each capable of increasing secretion amount of fab-type antibody
Shiota et al. The Tom40 assembly process probed using the attachment of different intramitochondrial sorting signals
US20120202244A1 (en) Antibodies Capable of Specifically Binding to a Specific Amino Acid Sequence
Shin Yeast as a Heterologous Expression System and structural characterisation of clinically relevant proteins
Kjærulff et al. Alternative protein secretion: the Mam1 ABC transporter supports secretion of M-factor linked GFP in fission yeast
Koubaa et al. Yarrowia Lipolytica Yeast: From Metabolic Engineering to Biotechnological Applications
Hsieh Engineering the yeast Kluyveromyces lactis for the high level expression of heterologous genes
EP4642916A2 (en) Secretion signals

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C02 Deemed withdrawal of patent application after publication (patent law 2001)
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20121128