JP2013510567A5 - - Google Patents

Download PDF

Info

Publication number
JP2013510567A5
JP2013510567A5 JP2012538468A JP2012538468A JP2013510567A5 JP 2013510567 A5 JP2013510567 A5 JP 2013510567A5 JP 2012538468 A JP2012538468 A JP 2012538468A JP 2012538468 A JP2012538468 A JP 2012538468A JP 2013510567 A5 JP2013510567 A5 JP 2013510567A5
Authority
JP
Japan
Prior art keywords
medium
stem cells
pluripotent stem
cells
concentration range
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2012538468A
Other languages
English (en)
Japanese (ja)
Other versions
JP6276918B2 (ja
JP2013510567A (ja
Filing date
Publication date
Application filed filed Critical
Priority claimed from PCT/IL2010/000937 external-priority patent/WO2011058558A2/en
Publication of JP2013510567A publication Critical patent/JP2013510567A/ja
Publication of JP2013510567A5 publication Critical patent/JP2013510567A5/ja
Application granted granted Critical
Publication of JP6276918B2 publication Critical patent/JP6276918B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

JP2012538468A 2009-11-12 2010-11-11 多能性幹細胞を未分化状態で培養する培地、細胞培養および方法 Active JP6276918B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US27286009P 2009-11-12 2009-11-12
US61/272,860 2009-11-12
PCT/IL2010/000937 WO2011058558A2 (en) 2009-11-12 2010-11-11 Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2015207773A Division JP2016047056A (ja) 2009-11-12 2015-10-22 多能性幹細胞を未分化状態で培養する培地、細胞培養および方法

Publications (3)

Publication Number Publication Date
JP2013510567A JP2013510567A (ja) 2013-03-28
JP2013510567A5 true JP2013510567A5 (cg-RX-API-DMAC7.html) 2013-12-26
JP6276918B2 JP6276918B2 (ja) 2018-02-07

Family

ID=43992162

Family Applications (5)

Application Number Title Priority Date Filing Date
JP2012538468A Active JP6276918B2 (ja) 2009-11-12 2010-11-11 多能性幹細胞を未分化状態で培養する培地、細胞培養および方法
JP2015207773A Pending JP2016047056A (ja) 2009-11-12 2015-10-22 多能性幹細胞を未分化状態で培養する培地、細胞培養および方法
JP2017152419A Active JP6603694B2 (ja) 2009-11-12 2017-08-07 多能性幹細胞を未分化状態で培養する培地、細胞培養および方法
JP2019188087A Active JP7053551B2 (ja) 2009-11-12 2019-10-11 多能性幹細胞を未分化状態で培養する培地、細胞培養および方法
JP2022059325A Active JP7383069B2 (ja) 2009-11-12 2022-03-31 多能性幹細胞を未分化状態で培養する培地、細胞培養および方法

Family Applications After (4)

Application Number Title Priority Date Filing Date
JP2015207773A Pending JP2016047056A (ja) 2009-11-12 2015-10-22 多能性幹細胞を未分化状態で培養する培地、細胞培養および方法
JP2017152419A Active JP6603694B2 (ja) 2009-11-12 2017-08-07 多能性幹細胞を未分化状態で培養する培地、細胞培養および方法
JP2019188087A Active JP7053551B2 (ja) 2009-11-12 2019-10-11 多能性幹細胞を未分化状態で培養する培地、細胞培養および方法
JP2022059325A Active JP7383069B2 (ja) 2009-11-12 2022-03-31 多能性幹細胞を未分化状態で培養する培地、細胞培養および方法

Country Status (8)

Country Link
US (4) US9018010B2 (cg-RX-API-DMAC7.html)
EP (3) EP3633025B1 (cg-RX-API-DMAC7.html)
JP (5) JP6276918B2 (cg-RX-API-DMAC7.html)
CA (3) CA3080762A1 (cg-RX-API-DMAC7.html)
DK (2) DK2499236T3 (cg-RX-API-DMAC7.html)
ES (2) ES2779048T3 (cg-RX-API-DMAC7.html)
IL (1) IL252607B (cg-RX-API-DMAC7.html)
WO (1) WO2011058558A2 (cg-RX-API-DMAC7.html)

Families Citing this family (46)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3633025B1 (en) * 2009-11-12 2022-09-14 Technion Research & Development Foundation Ltd. Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state
SG10201501503VA (en) 2010-03-01 2015-04-29 Janssen Biotech Inc Methods for purifying cells derived from pluripotent stem cells
US9279107B2 (en) 2010-08-05 2016-03-08 Wisconsin Alumni Research Foundation Simplified basic media for human pluripotent cell culture
CN108531444A (zh) 2010-08-05 2018-09-14 威斯康星校友研究基金会 用于人多能细胞培养的简化基础培养基
SG10201509953VA (en) 2010-09-07 2016-01-28 Technion Res & Dev Foundation Novel Methods And Culture Media For Culturing Pluripotent Stem Cells
KR101975688B1 (ko) 2010-12-22 2019-05-07 페이트 세러퓨틱스, 인코포레이티드 단세포 분류 및 iPSC의 증강된 재프로그래밍을 위한 세포 배양 플랫폼
EP4086338A1 (en) 2011-03-17 2022-11-09 Minerva Biotechnologies Corporation Method for making pluripotent stem cells
GB201117469D0 (en) * 2011-10-10 2011-11-23 Cell Guidance Systems Ltd Culture media for pluripotent stem cells
CN103998598A (zh) 2011-10-17 2014-08-20 米纳瓦生物技术公司 用于干细胞增殖和诱导的培养基
US20140329317A1 (en) 2011-11-25 2014-11-06 Kyoto University Method for culturing pluripotent stem cell
CA2858148C (en) 2011-12-05 2023-03-14 Factor Bioscience Inc. Methods and products for transfecting cells
US8497124B2 (en) 2011-12-05 2013-07-30 Factor Bioscience Inc. Methods and products for reprogramming cells to a less differentiated state
MX2014007744A (es) 2011-12-22 2015-01-12 Janssen Biotech Inc Diferenciacion de celulas madre embrionicas humanas en celulas individuales positivas de insulina hormonal.
BR112014027783A2 (pt) * 2012-05-07 2017-06-27 Janssen Biotech Inc diferenciação de células-tronco embrionárias humanas em endoderma pancreático
US10344264B2 (en) 2012-12-31 2019-07-09 Janssen Biotech, Inc. Culturing of human embryonic stem cells at the air-liquid interface for differentiation into pancreatic endocrine cells
RU2687379C2 (ru) 2012-12-31 2019-05-13 Янссен Байотек, Инк. Суспендирование и кластеризация плюрипотентных клеток человека с целью их дифференцировки в панкреатические эндокринные клетки
US10370644B2 (en) 2012-12-31 2019-08-06 Janssen Biotech, Inc. Method for making human pluripotent suspension cultures and cells derived therefrom
US9738861B2 (en) 2013-03-06 2017-08-22 Kyoto University Culture system for pluripotent stem cells and method for subculturing pluripotent stem cells
DK3008172T3 (da) * 2013-06-11 2020-07-20 Ncardia B V Dyrkningsmedium-sammensætninger til modning af cardiomyocytter afledt fra pluripotente mammale stamceller
WO2014208295A1 (ja) * 2013-06-28 2014-12-31 株式会社カネカ 多能性幹細胞の増殖促進因子のスクリーニング法
WO2015037623A1 (ja) * 2013-09-10 2015-03-19 富士フイルム株式会社 多能性幹細胞の培養方法ならびにこれに用いる多能性幹細胞の培養用キットおよび培地
WO2015037622A1 (ja) * 2013-09-10 2015-03-19 富士フイルム株式会社 多能性幹細胞の培養方法、培養用キット及び多能性幹細胞培養用培地
JP6516280B2 (ja) * 2013-12-26 2019-05-22 国立大学法人広島大学 iPS細胞の樹立方法および幹細胞の長期維持方法
WO2015098111A1 (ja) * 2013-12-26 2015-07-02 国立大学法人広島大学 iPS細胞の樹立方法および幹細胞の長期維持方法
KR102670874B1 (ko) * 2014-03-04 2024-05-31 페이트 세러퓨틱스, 인코포레이티드 개선된 재프로그래밍 방법 및 세포 배양 플랫폼
KR20160147800A (ko) * 2014-05-06 2016-12-23 에프. 호프만-라 로슈 아게 다능성 줄기세포의 심근세포로의 분화 방법
US20150335010A1 (en) 2014-05-21 2015-11-26 Bourbon Corporation Maintenance medium for primate pluripotent stem cells
CN104388382B (zh) * 2014-11-21 2017-05-10 吉林大学 利用搅拌式细胞培养转瓶机快速制备饲养层细胞的方法
US9944894B2 (en) 2015-01-16 2018-04-17 General Electric Company Pluripotent stem cell expansion and passage using a rocking platform bioreactor
CN108138130A (zh) * 2015-08-31 2018-06-08 爱平世股份有限公司 多能干细胞制造系统和生产诱导多能干细胞的方法
ES2971677T3 (es) * 2015-09-08 2024-06-06 Sumitomo Pharma Co Ltd Método para la producción de tejido retiniano
EP3362570A4 (en) 2015-10-16 2019-03-20 Fate Therapeutics, Inc. PLATFORM FOR THE INDUCTION AND MAINTENANCE OF BASIC STATUS PLURIPOTENCE
JP7019667B2 (ja) * 2016-07-19 2022-02-15 アクセルタ リミテッド 多能性幹細胞の懸濁培養のための培養培地
WO2018029656A2 (en) * 2016-08-12 2018-02-15 The University Of Chicago Methods for making and using therapeutic exosomes
US20200147146A1 (en) * 2017-05-02 2020-05-14 Koji Tanabe Pharmaceutical composition and cosmetic composition
EP3770249A4 (en) * 2018-03-20 2021-04-21 FUJIFILM Corporation METHOD OF THREE-DIMENSIONAL CULTIVATION OF PLURIPOTENT STEM CELLS
AU2020351221A1 (en) * 2019-09-19 2022-05-05 Northwestern University Cost effective culture media and protocol for human induced pluripotent stem cells
WO2021118226A1 (ko) * 2019-12-09 2021-06-17 주식회사 대웅제약 인간 만능줄기세포로부터 중간엽 줄기세포의 제조방법 및 이에 의해 제조된 중간엽 줄기세포
WO2021145319A1 (ja) * 2020-01-14 2021-07-22 味の素株式会社 細胞培養用培地組成物
CN111484970B (zh) * 2020-04-30 2022-09-16 广州再生医学与健康广东省实验室 一种低蛋白含量的无血清无饲养层胚胎与多能干细胞培养基
IL309185A (en) * 2021-06-09 2024-02-01 Accellta Ltd Serum-free media for suspension cultivation of pluripotent stem cells from mammalian farm animals
US11735303B2 (en) 2021-06-22 2023-08-22 David Haase Apparatus and method for determining a composition of a replacement therapy treatment
CN113403262A (zh) * 2021-06-23 2021-09-17 昆明理工大学 一种对食蟹猴干细胞的无饲养层培养方法
WO2023053220A1 (ja) * 2021-09-28 2023-04-06 公益財団法人京都大学iPS細胞研究財団 多能性幹細胞の製造方法
CA3262188A1 (en) * 2022-08-02 2024-02-08 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College BOVIN TROPHOBLASTE STEM CELLS AND THEIR USES
USD1069165S1 (en) 2022-11-02 2025-04-01 Abs Global, Inc. Dish holder

Family Cites Families (192)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL154600B (nl) 1971-02-10 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van specifiek bindende eiwitten en hun corresponderende bindbare stoffen.
NL154598B (nl) 1970-11-10 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van laagmoleculire verbindingen en van eiwitten die deze verbindingen specifiek kunnen binden, alsmede testverpakking.
NL154599B (nl) 1970-12-28 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van specifiek bindende eiwitten en hun corresponderende bindbare stoffen, alsmede testverpakking.
US3901654A (en) 1971-06-21 1975-08-26 Biological Developments Receptor assays of biologically active compounds employing biologically specific receptors
US3853987A (en) 1971-09-01 1974-12-10 W Dreyer Immunological reagent and radioimmuno assay
US3867517A (en) 1971-12-21 1975-02-18 Abbott Lab Direct radioimmunoassay for antigens and their antibodies
NL171930C (nl) 1972-05-11 1983-06-01 Akzo Nv Werkwijze voor het aantonen en bepalen van haptenen, alsmede testverpakkingen.
US3850578A (en) 1973-03-12 1974-11-26 H Mcconnell Process for assaying for biologically active molecules
US3935074A (en) 1973-12-17 1976-01-27 Syva Company Antibody steric hindrance immunoassay with two antibodies
US3996345A (en) 1974-08-12 1976-12-07 Syva Company Fluorescence quenching with immunological pairs in immunoassays
US4034074A (en) 1974-09-19 1977-07-05 The Board Of Trustees Of Leland Stanford Junior University Universal reagent 2-site immunoradiometric assay using labelled anti (IgG)
US3984533A (en) 1975-11-13 1976-10-05 General Electric Company Electrophoretic method of detecting antigen-antibody reaction
US4098876A (en) 1976-10-26 1978-07-04 Corning Glass Works Reverse sandwich immunoassay
US4879219A (en) 1980-09-19 1989-11-07 General Hospital Corporation Immunoassay utilizing monoclonal high affinity IgM antibodies
IL68218A (en) 1983-03-23 1985-12-31 Univ Ramot Compositions for cartilage repair comprising embryonal chondrocytes
US5011771A (en) 1984-04-12 1991-04-30 The General Hospital Corporation Multiepitopic immunometric assay
US4666828A (en) 1984-08-15 1987-05-19 The General Hospital Corporation Test for Huntington's disease
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4801531A (en) 1985-04-17 1989-01-31 Biotechnology Research Partners, Ltd. Apo AI/CIII genomic polymorphisms predictive of atherosclerosis
US5272057A (en) 1988-10-14 1993-12-21 Georgetown University Method of detecting a predisposition to cancer by the use of restriction fragment length polymorphism of the gene for human poly (ADP-ribose) polymerase
US5192659A (en) 1989-08-25 1993-03-09 Genetype Ag Intron sequence analysis method for detection of adjacent and remote locus alleles as haplotypes
US5281521A (en) 1992-07-20 1994-01-25 The Trustees Of The University Of Pennsylvania Modified avidin-biotin technique
US5489304A (en) 1994-04-19 1996-02-06 Brigham & Women's Hospital Method of skin regeneration using a collagen-glycosaminoglycan matrix and cultured epithelial autograft
US5843780A (en) 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
AU7243100A (en) 1995-02-02 2001-05-03 Tristem Trading (Cyprus) Limited A method of preparing an undifferentiated cell
US7410773B2 (en) 1995-02-02 2008-08-12 Ghazi Jaswinder Dhoot Method of preparing an undifferentiated cell
JPH08308561A (ja) * 1995-05-16 1996-11-26 Sumitomo Electric Ind Ltd 動物細胞培養用無血清培地
US5811297A (en) 1996-03-07 1998-09-22 Amba Biosciences, Llc Immortalized hematopoietic cell lines, cell system thereof with stromal cells, in vitro, ex vivo and in vivo uses, & in vitro generation of dendritic cells and macrophages
WO1997033995A2 (en) 1996-03-15 1997-09-18 Munin Corporation Extracellular matrix signalling molecules
US6878544B2 (en) 1996-04-19 2005-04-12 Neurotech Sa Retinal cell lines with extended life-span and their applications
WO1998030679A1 (en) 1997-01-10 1998-07-16 Life Technologies, Inc. Embryonic stem cell serum replacement
US6090622A (en) 1997-03-31 2000-07-18 The Johns Hopkins School Of Medicine Human embryonic pluripotent germ cells
US6331406B1 (en) 1997-03-31 2001-12-18 The John Hopkins University School Of Medicine Human enbryonic germ cell and methods of use
WO1999001763A2 (en) 1997-07-02 1999-01-14 Board Of Regents, The University Of Texas System p53 AS A REGULATOR OF CELL DIFFERENTIATION
IL122818A0 (en) 1997-07-10 1998-08-16 Yeda Res & Dev Chimeric interleukin-6 soluble receptor/ligand protein analogs thereof and uses thereof
AU729377B2 (en) 1997-10-23 2001-02-01 Asterias Biotherapeutics, Inc. Methods and materials for the growth of primate-derived primordial stem cells in feeder-free culture
US6667176B1 (en) 2000-01-11 2003-12-23 Geron Corporation cDNA libraries reflecting gene expression during growth and differentiation of human pluripotent stem cells
US7413904B2 (en) 1998-10-23 2008-08-19 Geron Corporation Human embryonic stem cells having genetic modifications
US7410798B2 (en) 2001-01-10 2008-08-12 Geron Corporation Culture system for rapid expansion of human embryonic stem cells
EP1129176A4 (en) 1998-11-09 2002-10-30 Es Cell Int Pte Ltd EMBRYONIC STEM CELLS
ATE342348T1 (de) * 1998-11-09 2006-11-15 Consorzio Per La Gestione Del Serum-freies medium für chondrozyt-ähnliche zellen
US20030129745A1 (en) 1999-10-28 2003-07-10 Robl James M. Gynogenetic or androgenetic production of pluripotent cells and cell lines, and use thereof to produce differentiated cells and tissues
US7455983B2 (en) 2000-01-11 2008-11-25 Geron Corporation Medium for growing human embryonic stem cells
JP2001229797A (ja) 2000-02-15 2001-08-24 Yazaki Corp ガス式電源遮断装置
US7514260B2 (en) 2004-05-21 2009-04-07 Wicell Research Institute, Inc. Feeder independent extended culture of embryonic stem cells
EP1263932A4 (en) 2000-03-14 2004-06-23 Es Cell Int Pte Ltd EMBRYONIC CELLS AND NEURONAL STEM CELLS DERIVED THEREFROM
CA2399399A1 (en) 2000-03-30 2001-10-11 Oxford Biomedica (Uk) Limited Retinoic acid receptor beta-2, its antagonists, and gene therapy vectors for the treatment of neurological disorders
US7473555B2 (en) 2000-04-27 2009-01-06 Geron Corporation Protocols for making hepatocytes from embryonic stem cells
NL1017973C2 (nl) 2000-05-10 2002-11-08 Tristem Trading Cyprus Ltd Inrichting.
US7250294B2 (en) 2000-05-17 2007-07-31 Geron Corporation Screening small molecule drugs using neural cells differentiated from human embryonic stem cells
GB2379447B (en) 2000-05-17 2004-12-29 Geron Corp Neural progenitor cell populations
AU6842001A (en) 2000-06-15 2001-12-24 Tanja Dominko Pluripotent mammalian cells
GB0025088D0 (en) 2000-10-13 2000-11-29 Univ Edinburgh Stem cells
US6576464B2 (en) 2000-11-27 2003-06-10 Geron Corporation Methods for providing differentiated stem cells
CA2430653A1 (en) 2000-11-27 2002-08-08 Yissum Research Development Company Of The Hebrew University In Jerusalem Transfection of human embryonic stem cells
CA2430627A1 (en) 2000-11-30 2002-12-27 Stemron, Inc. Isolated homozygous stem cells differentiated cells derived therefrom and materials and methods for making and using same
JP2004531262A (ja) 2001-05-15 2004-10-14 ラッパポート ファミリー インスチチュート フォア リサーチ イン ザ メディカル サイエンシズ ヒト胚性幹細胞由来インスリン産生細胞
AU2002322379B2 (en) 2001-07-06 2007-02-15 Asterias Biotherapeutics, Inc. Mesenchymal cells and osteoblasts from human embryonic stem cell
KR101073411B1 (ko) 2001-07-12 2011-10-17 제론 코포레이션 인간 다분화능 줄기 세포로부터 제조된 심근 세포 계통의 세포
AU2002317039B2 (en) 2001-07-24 2007-10-04 Es Cell International Pte Ltd Methods of inducing differentiation of stem cells
CA2456981C (en) 2001-08-06 2012-02-28 Bresagen, Inc. Alternative compositions and methods for the culture of stem cells
US20030211603A1 (en) 2001-08-14 2003-11-13 Earp David J. Reprogramming cells for enhanced differentiation capacity using pluripotent stem cells
WO2003018780A1 (en) 2001-08-27 2003-03-06 Advanced Cell Technology, Inc. De-differentiation and re-differentiation of somatic cells and production of cells for cell therapies
EP1425388A2 (en) 2001-09-12 2004-06-09 DeveloGen Aktiengesellschaft für entwicklungsbiologische Forschung A method for isolating, culturing and differentiating intestinal stem cells for therapeutic use
US20050095708A1 (en) 2001-11-09 2005-05-05 Pera Martin F. Characterization and isolation of subsets of human embryonic stem cells (HES) and cells associated or derived therefrom
IL162132A0 (en) 2001-12-07 2005-11-20 Geron Corp Chondrocyte precursors derived from human embryonic stem cells
IL162130A0 (en) 2001-12-07 2005-11-20 Robarts Res Inst Hematopoietic cells from human embryonic stem cells
CN1602351B (zh) 2001-12-07 2011-03-30 杰龙公司 源自人胚胎干细胞的胰岛细胞
DE60239420D1 (de) 2001-12-21 2011-04-21 Thrombogenics Nv Zusammensetzungen für die in vitro herleitung und kultur embryonaler stammzellinien mit keimbahnübertragungsfähigkeit und für die kultur adulter stammzellen
CN1671835A (zh) 2001-12-28 2005-09-21 塞拉提斯股份公司 一种建立多能人胚泡衍生的干细胞的方法
AU2009213101B2 (en) 2002-06-05 2012-03-08 Es Cell International Pte Ltd Generation of neural stem cells from undifferentiated human embryonic stem cells
US7410797B2 (en) 2002-06-11 2008-08-12 Ogle Roy C Meningeal-derived stem cells
US7662625B2 (en) 2002-07-02 2010-02-16 Cancer Research Technology Limited Methods for detecting the differentiation status of cells using 5T4 antigen expression
US7285415B2 (en) 2002-07-11 2007-10-23 The Regents Of The University Of California Oligodendrocytes derived from human embryonic stem cells for remyelination and treatment of spinal cord injury
US20050101014A1 (en) 2002-07-11 2005-05-12 Keirstead Hans S. Oligodendrocytes derived from human embryonic stem cells for remyelination and treatment of spinal cord injury
US7267981B2 (en) 2002-10-07 2007-09-11 Technion Research & Development Foundation Ltd. Human foreskin fibroblasts for culturing ES cells
AU2003285172A1 (en) 2002-11-08 2004-06-03 The Johns Hopkins University Human embryonic stem cell cultures, and compositions and methods for growing same
US20040110286A1 (en) 2002-12-06 2004-06-10 The John P. Robarts Research Institute Method for making hematopoietic cells
PT2457999T (pt) 2002-12-16 2019-01-28 Technion Res & Dev Foundation Sistema de cultura para células estaminais pluripotentes
WO2004078944A2 (en) 2003-03-05 2004-09-16 Maine Medical Center Research Institute Thrombin, soluble jaggedi, and trap and growth of stem cells
US20060134636A1 (en) 2003-03-13 2006-06-22 Stanton Lawrence W Standardization of growth conditions for human embryonic stem cells intended for use in regenerative medicine
US20030224411A1 (en) 2003-03-13 2003-12-04 Stanton Lawrence W. Genes that are up- or down-regulated during differentiation of human embryonic stem cells
US7153650B2 (en) 2003-03-13 2006-12-26 Geron Corporation Marker system for preparing and characterizing high-quality human embryonic stem cells
US20070053890A1 (en) 2003-03-28 2007-03-08 Sunnybrook And Women's College Health Sciences Centre Canine embryonic stem cells
JP5426073B2 (ja) 2003-05-08 2014-02-26 セルアーティス アーベー ヒト胚盤胞由来幹細胞(hBS細胞)のフィーダー支持からフィーダーなしの培養系への効率的な移送方法
US20050118713A1 (en) 2003-05-12 2005-06-02 Nikolai Strelchenko Morula derived embryonic stem cells
US20040229350A1 (en) 2003-05-12 2004-11-18 Nikolai Strelchenko Morula derived embryonic stem cells
US7569385B2 (en) 2003-08-14 2009-08-04 The Regents Of The University Of California Multipotent amniotic fetal stem cells
CA2549158A1 (en) 2003-12-01 2005-06-16 Technion Research And Development Foundation Ltd. Methods of generating stem cells and embryonic bodies carrying disease-causing mutations and methods of using same for studying genetic disorders
TWI280280B (en) 2003-12-09 2007-05-01 Ind Tech Res Inst Culture system and method for expansion and undifferentiated growth of human embryonic stem cells
US20050233446A1 (en) 2003-12-31 2005-10-20 Parsons Xuejun H Defined media for stem cell culture
WO2005080551A2 (en) 2004-02-12 2005-09-01 University Of Newcastle Upon Tyne Stem cells
US20060216821A1 (en) 2004-02-26 2006-09-28 Reliance Life Sciences Pvt. Ltd. Pluripotent embryonic-like stem cells derived from corneal limbus, methods of isolation and uses thereof
EP1730261A4 (en) 2004-03-10 2007-11-28 Univ California COMPOSITIONS AND METHODS FOR GROWING EMBRYONIC STEM CELLS
IL178006A (en) 2004-03-19 2011-08-31 Geron Corp Method for making high purity cardiomyocyte preparations suitable for regenerative medicine
CA2559854C (en) 2004-03-19 2014-12-02 Geron Corporation Method for making high purity cardiomyocyte preparations suitable for regenerative medicine
CA2560581C (en) 2004-03-23 2013-06-11 Toshihiro Akaike Pluripotent stem cell growing method
US7452718B2 (en) 2004-03-26 2008-11-18 Geron Corporation Direct differentiation method for making cardiomyocytes from human embryonic stem cells
US20050214938A1 (en) 2004-03-26 2005-09-29 Gold Joseph D Cardiac bodies: clusters of spontaneously contracting cells for regenerating cardiac function
US20050227352A1 (en) 2004-04-09 2005-10-13 Stowers Institute For Medical Research Use of BMP (BAM) signaling to control stem cell fate determination
US9375514B2 (en) 2004-05-21 2016-06-28 Fraunhofer-Gesellschaft Zur Forderung Der Angewandten Forschung E.V. Multicellular tissue and organ culture systems
US20080038820A1 (en) 2004-06-22 2008-02-14 Rudy-Reil Diane E Induction of pluripotent stem cells into mesodermal lineages
US20090136559A1 (en) 2004-07-09 2009-05-28 Athanasiou Kyriacos A Chondrocyte Differentiation from Human Embryonic Stem Cells and Their Use in Tissue Engineering
WO2006017370A2 (en) 2004-07-13 2006-02-16 Geron Corporation Medium for growing human embryonic stem cells
CA2504451A1 (en) 2004-08-10 2006-02-10 Geron Corporation Dendritic cell vaccines for treating cancer made from embryonic stem cells
WO2006017855A2 (en) 2004-08-12 2006-02-16 Board Of Regents Of The University Of Texas System Methods and compositions for regulation of stem cell survival, proliferation, and differentiation by protein ubiquitination
DE102004043256B4 (de) 2004-09-07 2013-09-19 Rheinische Friedrich-Wilhelms-Universität Bonn Skalierbarer Prozess zur Kultivierung undifferenzierter Stammzellen in Suspension
US7442548B2 (en) 2004-09-08 2008-10-28 Wisconsin Alumni Research Foundation Culturing human embryonic stem cells in medium containing pipecholic acid and gamma amino butyric acid
WO2006033103A2 (en) 2004-09-21 2006-03-30 Nvr Labs. Ltd. Compositions and methods for stem cell expansion and differentiation
WO2006040763A2 (en) 2004-10-12 2006-04-20 Technion Research & Development Foundation Ltd. Isolated primate embryonic cells and methods of generating and using same
JP4646600B2 (ja) * 2004-11-02 2011-03-09 オリンパス株式会社 間葉系幹細胞の培養方法
US7641897B2 (en) 2004-11-23 2010-01-05 The Board Of Trustees Of The Leland Stanford Junior University Feeder layer and serum independent embryonic stem cells
FR2878860A1 (fr) 2004-12-08 2006-06-09 Vivalis Sa Lignees de cellules souches humaines derivees de cellules es et utilisations pour la production de vaccins et de proteines recombinantes
WO2006070370A2 (en) 2004-12-29 2006-07-06 Hadasit Medical Research Services & Development Limited Stem cells culture systems
US8597947B2 (en) 2004-12-29 2013-12-03 Hadasit Medical Research Services & Development Limited Undifferentiated stem cell culture systems
GB2437689B (en) 2005-01-28 2009-10-28 Novathera Ltd Culture and screening methods
WO2006088867A2 (en) 2005-02-15 2006-08-24 Medistem Laboratories, Incorporated Method for expansion of stem cells
JP2008531001A (ja) 2005-02-23 2008-08-14 エージェンシー・フォー・サイエンス・テクノロジー・アンド・リサーチ 不死化フィーダー細胞
US8012751B2 (en) 2005-03-31 2011-09-06 Wisconsin Alumni Research Foundation Differentiation of pluripotent embryonic stem cells
JP4755460B2 (ja) * 2005-07-27 2011-08-24 オリンパス株式会社 間葉系幹細胞の培養方法
EP2210937A1 (en) 2005-05-09 2010-07-28 Olympus Corporation Culture method for mesenchymal stem cell and process for production of supporting material for biological tissue
US9446073B2 (en) 2005-05-15 2016-09-20 Biodontos, Llc Non-lineage committed precursor cells from the dental papillary tissue of teeth
WO2007020655A1 (en) 2005-05-17 2007-02-22 Reliance Life Sciences Pvt Ltd Establishment of a human embryonic stem cell line using mammalian cells
US20110311977A1 (en) 2005-05-17 2011-12-22 Arundhati Mandal In Vitro Generation of Hepatocytes from Human Embryonic Stem Cells
WO2007002210A2 (en) 2005-06-20 2007-01-04 Bresagen, Inc. Embryonic stem cell culture compositions and methods of use thereof
KR20130100221A (ko) 2005-06-22 2013-09-09 제론 코포레이션 인간 배아 줄기 세포의 현탁 배양
JP2009502124A (ja) 2005-07-15 2009-01-29 プライムジェン バイオテック エルエルシー 生殖系列幹細胞の療法的再プログラミング
EP3354723B1 (en) 2005-08-29 2023-12-13 Technion Research & Development Foundation Ltd. Media for culturing stem cells
US7754483B2 (en) 2005-11-09 2010-07-13 The Penn State Research Foundation Systems and methods for selection and maintenance of homogeneous and pluripotent human embryonic stem cells
US20080070303A1 (en) 2005-11-21 2008-03-20 West Michael D Methods to accelerate the isolation of novel cell strains from pluripotent stem cells and cells obtained thereby
CN101864392B (zh) 2005-12-13 2016-03-23 国立大学法人京都大学 核重新编程因子
US20070249044A1 (en) 2006-01-30 2007-10-25 University Of Illinois At Chicago Microstructures in three dimensional gel suspensions for growth of cells
EP1983042B1 (en) 2006-01-31 2017-03-15 Daiichi Sankyo Company, Limited A method for purifying cardiomyocytes or programmed cardiomyocytes derived from stem cells or fetuses
CA2643478C (en) * 2006-02-23 2019-06-18 Novocell, Inc. Compositions and methods useful for culturing differentiable cells
JP2007228815A (ja) 2006-02-27 2007-09-13 Gifu Univ 胚性幹細胞の維持方法
WO2007122233A1 (en) 2006-04-25 2007-11-01 Vrije Universiteit Brussel Preparation of mesenchymal progenitor cells, particularly osteogenic progenitor cells
US8241908B2 (en) 2006-05-16 2012-08-14 Becton, Dickinson And Company Extracellular matrix coated surface for culturing cells
US9096831B2 (en) 2006-05-19 2015-08-04 Versitech Limited Methods to enhance cell migration and engraftment
GB0613756D0 (en) 2006-07-12 2006-08-23 Univ Sheffield Cell culture medium
EP2733203B1 (en) * 2006-08-02 2018-10-10 Technion Research & Development Foundation Ltd. Methods of expanding embryonic stem cells in a suspension culture
WO2008033469A1 (en) 2006-09-15 2008-03-20 Children's Medical Center Corporation Methods for producing embryonic stem cells from parthenogenetic embryos
US20100166714A1 (en) 2006-11-02 2010-07-01 The General Hospital Corporation Cardiovascular stem cells, methods for stem cell isolation, and uses thereof
US8895303B2 (en) 2006-11-13 2014-11-25 Charite-Universitatsmedizin Berlin Method of cell culture and method of treatment comprising a vEPO protein variant
US8414928B2 (en) 2006-12-06 2013-04-09 George R. Dodge Methods for the generation of cartilage-like material by mechanical loading
WO2008075378A2 (en) 2006-12-19 2008-06-26 Stempeutics Research Private Limited Xeno-free culture conditions for human embryonic stem cells and methods thereof
US20080182328A1 (en) 2006-12-19 2008-07-31 The Burnham Institute Mammalian extraembryonic endoderm cells and methods of isolation
US10829733B2 (en) 2007-01-04 2020-11-10 Biolamina Ab Composition and method for enabling proliferation of pluripotent human stem cells
US8722405B2 (en) 2007-01-04 2014-05-13 Karl Tryggvason Composition and method for enabling proliferation of pluripotent stem cells
CA2675445C (en) 2007-01-17 2014-11-04 Wisconsin Alumni Research Foundation Improved culture of stem cells
CA2676323A1 (en) 2007-01-18 2008-07-24 Suomen Punainen Risti, Veripalvelu Novel methods and reagents directed to production of cells
US20110033928A1 (en) 2007-03-09 2011-02-10 The Regents Of The University Of Michigan Methods and compositions for growth of cells and embryonic tissue on a synthetic polymer matrix
DE102007020302B4 (de) 2007-04-20 2012-03-22 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Verbesserte dreidimensionale biokompatible Gerüststruktur, die Nanopartikel beinhaltet
US20090053182A1 (en) 2007-05-25 2009-02-26 Medistem Laboratories, Inc. Endometrial stem cells and methods of making and using same
FI20075417A0 (fi) * 2007-06-05 2007-06-05 Marjo-Riitta Suuronen Koostumuksia ja menetelmiä alkion kantasolujen kasvatukseen
JP2008307007A (ja) * 2007-06-15 2008-12-25 Bayer Schering Pharma Ag 出生後のヒト組織由来未分化幹細胞から誘導したヒト多能性幹細胞
DK2173863T3 (en) 2007-06-29 2019-01-21 Fujifilm Cellular Dynamics Inc Automated method and apparatus for embryonic stem cell culture
EP2028268A1 (en) 2007-08-20 2009-02-25 Université Libre De Bruxelles Generation of neuronal cells from pluripotent stem cells
US20110045996A1 (en) 2007-08-21 2011-02-24 Gene Yeo Robust regression based exon array protocol system and applications
US8623650B2 (en) 2007-10-19 2014-01-07 Viacyte, Inc. Methods and compositions for feeder-free pluripotent stem cell media containing human serum
EP2072618A1 (en) 2007-12-14 2009-06-24 Johannes Gutenberg-Universität Mainz Use of RNA for reprogramming somatic cells
AU2008338989A1 (en) 2007-12-17 2009-06-25 Gliamed, Inc. Stem-like cells and method for reprogramming adult mammalian somatic cells
CA2713754A1 (en) 2008-01-30 2009-08-13 Geron Corporation Synthetic surfaces for culturing cells in chemically defined media
EP2247718B1 (en) * 2008-01-30 2019-01-16 Asterias Biotherapeutics, Inc. Synthetic surfaces for culturing stem cell derived cardiomyocytes
WO2009150051A2 (en) 2008-05-27 2009-12-17 Aarhus Universitet Biocompatible materials for mammalian stem cell growth and differentiation
US20100120142A1 (en) 2008-07-11 2010-05-13 Suomen Punainen Risti Veripalvelu Culture of human embryonic cells
US20100035327A1 (en) 2008-08-11 2010-02-11 Ann Marie Steele Use of rice-derived products in a universal cell culture medium
SG160248A1 (en) 2008-09-18 2010-04-29 Agency Science Tech & Res Use of novel monoclonal antibodies targeting human embryonic stem cells to characterize and kill induced pluripotent stem cells
US8895300B2 (en) 2008-11-04 2014-11-25 Viacyte, Inc. Scalable primate pluripotent stem cell aggregate suspension culture and differentiation thereof
US8008075B2 (en) 2008-11-04 2011-08-30 Viacyte, Inc. Stem cell aggregate suspension compositions and methods of differentiation thereof
US8956867B2 (en) 2008-11-07 2015-02-17 Wisconsin Alumni Research Foundation Method for culturing stem cells
EP2401364B1 (en) 2009-02-27 2015-04-22 Cellular Dynamics International, Inc. Differentiation of pluripotent cells
SG175367A1 (en) 2009-04-27 2011-11-28 Viacyte Inc Small molecules supporting pluripotent cell growth and methods thereof
JPWO2010134619A1 (ja) 2009-05-18 2012-11-12 国立大学法人東北大学 人工多能性幹細胞からの上皮系前駆細胞・幹細胞群及び角膜上皮細胞群の分化誘導方法
KR20170075023A (ko) 2009-06-11 2017-06-30 미네르바 바이오테크놀로지 코포레이션 줄기 세포 및 전구 세포를 배양하는 방법
KR20120065966A (ko) 2009-06-12 2012-06-21 난양 폴리테크닉 신규한 용도
KR101166257B1 (ko) 2009-06-16 2012-07-19 한국생명공학연구원 미분화 다능성줄기세포의 제작, 유지 및 증식을 위한 뉴로펩타이드 y를 포함하는 배지 조성물 및 이를 이용한 다능성줄기세포의 배양 방법
US20110039333A1 (en) 2009-08-12 2011-02-17 University Of Southern California Defined conditions for human embryonic stem cell culture and passage
EP4364797A3 (en) 2009-10-19 2024-07-10 FUJIFILM Cellular Dynamics, Inc. Cardiomyocyte production
EP3633025B1 (en) 2009-11-12 2022-09-14 Technion Research & Development Foundation Ltd. Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state
WO2011063005A2 (en) 2009-11-17 2011-05-26 Advanced Cell Technology, Inc. Methods of producing human rpe cells and pharmaceutical preparations of human rpe cells
CA2784847C (en) 2009-12-18 2017-11-21 Molly Sandra Shoichet Injectable polymer composition for use as a cell delivery vehicle
WO2011075686A2 (en) 2009-12-18 2011-06-23 Ventria Bioscience Methods & compositions comprising heat shock proteins
US20140030236A1 (en) 2010-02-25 2014-01-30 The Johns Hopkins University Derivation and maturation of synthetic and contractile vascular smooth muscle cells from human pluripotent stem cells
WO2011110886A1 (en) 2010-03-09 2011-09-15 Biolamina Ab Composition and method for enabling proliferation of pluripotent human stem cells
CA2796888A1 (en) 2010-04-20 2011-10-27 International Stem Cell Corporation Physiological methods for isolation of high purity cell populations
WO2011140397A2 (en) 2010-05-05 2011-11-10 The Regents Of The University Of California Office Of The President Stem cell defined media for xeno-free and feeder free conditions and uses thereof
US11786636B2 (en) 2010-06-15 2023-10-17 Versitech Limited Methods for complex tissue engineering
US9365826B2 (en) 2010-06-18 2016-06-14 Cellular Dynamics International, Inc. Cardiomyocyte medium with dialyzed serum
EP4524568A3 (en) 2010-07-23 2025-06-18 Astellas Institute for Regenerative Medicine Methods for detection of rare subpopulations of cells and highly purified compositions of cells
US20120141432A1 (en) 2010-09-03 2012-06-07 University Of Pittsburgh- Of The Commonwealth System Of Higher Education Use of catalytic antioxidant to preserve stem cell phenotype and control cell differentiation
SG10201509953VA (en) 2010-09-07 2016-01-28 Technion Res & Dev Foundation Novel Methods And Culture Media For Culturing Pluripotent Stem Cells
WO2012034101A2 (en) 2010-09-09 2012-03-15 The Regents Of The University Of California Expandable cell source of neuronal stem cell populations and methods for obtaining and using them

Similar Documents

Publication Publication Date Title
JP2013510567A5 (cg-RX-API-DMAC7.html)
GB2433943A (en) Cultivation of primate embryonic stem cells
JP5227318B2 (ja) 細胞増殖培地
JP2022088599A5 (cg-RX-API-DMAC7.html)
EP2970912B1 (en) Methods and materials for hematoendothelial differentiation of human pluripotent stem cells under defined conditions
WO2011058558A3 (en) Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state
JP2011206064A5 (cg-RX-API-DMAC7.html)
JP2003525625A5 (cg-RX-API-DMAC7.html)
WO2001051616A3 (en) Techniques for growth and differentiation of human pluripotent stem cells
CA2402299A1 (en) Serum free cultivation of primate embryonic stem cells
JP2004248507A5 (cg-RX-API-DMAC7.html)
JP2013521795A5 (cg-RX-API-DMAC7.html)
WO2015042356A1 (en) Chemically defined culture medium for stem cell maintenance and differentiation
Ning et al. Differentiation of mouse embryonic stem cells into dental epithelial-like cells induced by ameloblasts serum-free conditioned medium
JPWO2014168157A1 (ja) 肝幹前駆様細胞の培養方法及びその培養物
Boehm et al. The potential of human peripheral blood derived CD34+ cells for ex vivo red blood cell production
KR101175175B1 (ko) 인간 줄기세포에서 고활성 줄기세포를 분리하는 방법 및상기 방법에 의해 분리된 고활성 줄기 세포
CN1962855A (zh) 一种构建干细胞体外增殖分化微环境的方法
US20150329826A1 (en) Materials and methods for cell culture
KR100744445B1 (ko) 인간 배아 줄기 세포의 배양방법
CN100465268C (zh) 人胚胎干细胞的培养方法及其专用培养基
WO2011016485A1 (ja) iPS細胞から肝実質細胞への分化誘導方法
JP2007306856A (ja) 細胞凍結保存方法
US20210115395A1 (en) Methods for expanding adherent stromal cells and cells obtained thereby
JP4317337B2 (ja) 細胞株継代用酵素溶液、および、それを用いた霊長類胚性幹細胞の培養増殖方法