JP2013505740A5 - - Google Patents

Info

Publication number

JP2013505740A5

JP2013505740A5 JP2012532274A JP2012532274A JP2013505740A5 JP 2013505740 A5 JP2013505740 A5 JP 2013505740A5 JP 2012532274 A JP2012532274 A JP 2012532274A JP 2012532274 A JP2012532274 A JP 2012532274A JP 2013505740 A5 JP2013505740 A5 JP 2013505740A5

Authority

JP

Japan

Prior art keywords

polypeptide

yeast cell

plasmid

kari

cell

Prior art date

2010-09-29

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Granted

Links

• Espacenet
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• 210000005253 yeast cell Anatomy 0.000 claims description 19
• ZXEKIIBDNHEJCQ-UHFFFAOYSA-N isobutanol Chemical compound CC(C)CO ZXEKIIBDNHEJCQ-UHFFFAOYSA-N 0.000 claims description 18
• 210000004027 cell Anatomy 0.000 claims description 17
• 229920001184 polypeptide Polymers 0.000 claims description 17
• 108090000765 processed proteins & peptides Proteins 0.000 claims description 17
• 102000004196 processed proteins & peptides Human genes 0.000 claims description 17
• 230000000694 effects Effects 0.000 claims description 11
• 230000000813 microbial effect Effects 0.000 claims description 11
• 238000000034 method Methods 0.000 claims description 9
• 239000013612 plasmid Substances 0.000 claims description 9
• 101710148054 Ketol-acid reductoisomerase (NAD(+)) Proteins 0.000 claims description 8
• 101710099070 Ketol-acid reductoisomerase (NAD(P)(+)) Proteins 0.000 claims description 8
• 101710151482 Ketol-acid reductoisomerase (NADP(+)) Proteins 0.000 claims description 8
• 108020004707 nucleic acids Proteins 0.000 claims description 8
• 102000039446 nucleic acids Human genes 0.000 claims description 8
• 150000007523 nucleic acids Chemical class 0.000 claims description 8
• 108091026890 Coding region Proteins 0.000 claims description 5
• 108010000200 Ketol-acid reductoisomerase Proteins 0.000 claims description 5
• WTLNOANVTIKPEE-UHFFFAOYSA-N 2-acetyloxypropanoic acid Chemical compound OC(=O)C(C)OC(C)=O WTLNOANVTIKPEE-UHFFFAOYSA-N 0.000 claims description 4
• 108010011939 Pyruvate Decarboxylase Proteins 0.000 claims description 4
• 108090000623 proteins and genes Proteins 0.000 claims description 4
• 150000004728 pyruvic acid derivatives Chemical class 0.000 claims description 4
• CTMXBOCTJPQVDZ-UHFFFAOYSA-N 2,2-dihydroxy-3-methylbutanoic acid Chemical compound CC(C)C(O)(O)C(O)=O CTMXBOCTJPQVDZ-UHFFFAOYSA-N 0.000 claims description 2
• JTEYKUFKXGDTEU-UHFFFAOYSA-N 2,3-dihydroxy-3-methylbutanoic acid Chemical compound CC(C)(O)C(O)C(O)=O JTEYKUFKXGDTEU-UHFFFAOYSA-N 0.000 claims description 2
• 230000006696 biosynthetic metabolic pathway Effects 0.000 claims description 2
• 238000004519 manufacturing process Methods 0.000 claims description 2
• 241000194033 Enterococcus Species 0.000 description 5
• 241000186660 Lactobacillus Species 0.000 description 5
• 241000194036 Lactococcus Species 0.000 description 5
• 241000192132 Leuconostoc Species 0.000 description 5
• 241000194017 Streptococcus Species 0.000 description 5
• 229940039696 lactobacillus Drugs 0.000 description 5
• 241000894006 Bacteria Species 0.000 description 4
• 241000186781 Listeria Species 0.000 description 4
• 241000973040 Macrococcus Species 0.000 description 4
• 241000235648 Pichia Species 0.000 description 4
• 240000004808 Saccharomyces cerevisiae Species 0.000 description 4
• 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 4
• 241000191940 Staphylococcus Species 0.000 description 4
• 125000003275 alpha amino acid group Chemical group 0.000 description 3
• FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical group C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 2
• 241000222120 Candida <Saccharomycetales> Species 0.000 description 2
• LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
• 241000235649 Kluyveromyces Species 0.000 description 2
• 241000192001 Pediococcus Species 0.000 description 2
• 241000235070 Saccharomyces Species 0.000 description 2
• 241000235346 Schizosaccharomyces Species 0.000 description 2
• 241000235013 Yarrowia Species 0.000 description 2
• 230000001580 bacterial effect Effects 0.000 description 2
• 241000193830 Bacillus <bacterium> Species 0.000 description 1
• 241000186146 Brevibacterium Species 0.000 description 1
• 241000193403 Clostridium Species 0.000 description 1
• 241000186216 Corynebacterium Species 0.000 description 1
• 241000588722 Escherichia Species 0.000 description 1
• WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
• 101150111679 ILV5 gene Proteins 0.000 description 1
• 241000588748 Klebsiella Species 0.000 description 1
• 241000192129 Leuconostoc lactis Species 0.000 description 1
• 241000202223 Oenococcus Species 0.000 description 1
• 241000589516 Pseudomonas Species 0.000 description 1
• 241000589540 Pseudomonas fluorescens Species 0.000 description 1
• 241000316848 Rhodococcus <scale insect> Species 0.000 description 1
• 241000607142 Salmonella Species 0.000 description 1
• 101100125907 Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) ilvC1 gene Proteins 0.000 description 1
• 241000588901 Zymomonas Species 0.000 description 1
• 238000004458 analytical method Methods 0.000 description 1
• 239000008103 glucose Substances 0.000 description 1
• 238000004128 high performance liquid chromatography Methods 0.000 description 1
• 101150090497 ilvC gene Proteins 0.000 description 1
• 238000005070 sampling Methods 0.000 description 1