JP2013505025A5 - - Google Patents

Download PDF

Info

Publication number
JP2013505025A5
JP2013505025A5 JP2012530059A JP2012530059A JP2013505025A5 JP 2013505025 A5 JP2013505025 A5 JP 2013505025A5 JP 2012530059 A JP2012530059 A JP 2012530059A JP 2012530059 A JP2012530059 A JP 2012530059A JP 2013505025 A5 JP2013505025 A5 JP 2013505025A5
Authority
JP
Japan
Prior art keywords
plant
vlp
fraction
nucleic acid
apoplast
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2012530059A
Other languages
English (en)
Japanese (ja)
Other versions
JP5554414B2 (ja
JP2013505025A (ja
Filing date
Publication date
Application filed filed Critical
Priority claimed from PCT/CA2010/001488 external-priority patent/WO2011035422A1/en
Publication of JP2013505025A publication Critical patent/JP2013505025A/ja
Publication of JP2013505025A5 publication Critical patent/JP2013505025A5/ja
Application granted granted Critical
Publication of JP5554414B2 publication Critical patent/JP5554414B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

JP2012530059A 2009-09-22 2010-09-21 植物由来のvlpを調製する方法 Active JP5554414B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US24478609P 2009-09-22 2009-09-22
US61/244,786 2009-09-22
PCT/CA2010/001488 WO2011035422A1 (en) 2009-09-22 2010-09-21 Method of preparing plant-derived vlps

Publications (3)

Publication Number Publication Date
JP2013505025A JP2013505025A (ja) 2013-02-14
JP2013505025A5 true JP2013505025A5 (https=) 2013-06-27
JP5554414B2 JP5554414B2 (ja) 2014-07-23

Family

ID=43795254

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2012530060A Active JP5551780B2 (ja) 2009-09-22 2010-09-21 植物由来のタンパク質を調製する方法
JP2012530059A Active JP5554414B2 (ja) 2009-09-22 2010-09-21 植物由来のvlpを調製する方法

Family Applications Before (1)

Application Number Title Priority Date Filing Date
JP2012530060A Active JP5551780B2 (ja) 2009-09-22 2010-09-21 植物由来のタンパク質を調製する方法

Country Status (28)

Country Link
US (2) US11833200B2 (https=)
EP (3) EP2480560B1 (https=)
JP (2) JP5551780B2 (https=)
KR (4) KR101773431B1 (https=)
CN (4) CN107090021A (https=)
AR (2) AR078432A1 (https=)
AU (2) AU2010300033B2 (https=)
BR (2) BR112012006414B1 (https=)
CA (2) CA2772962C (https=)
DK (3) DK2480658T3 (https=)
ES (3) ES2911037T3 (https=)
HR (3) HRP20220478T1 (https=)
HU (3) HUE058165T2 (https=)
IL (4) IL218393A0 (https=)
IN (2) IN2012DN02637A (https=)
MX (2) MX2012003372A (https=)
MY (2) MY158475A (https=)
NO (2) NO2480658T3 (https=)
NZ (2) NZ598481A (https=)
PH (2) PH12012500566A1 (https=)
PL (3) PL2480658T3 (https=)
PT (3) PT3354657T (https=)
RU (2) RU2579903C2 (https=)
SG (2) SG178918A1 (https=)
SI (3) SI2480658T1 (https=)
TR (1) TR201803560T4 (https=)
WO (2) WO2011035422A1 (https=)
ZA (2) ZA201202836B (https=)

Families Citing this family (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2615372A1 (en) 2007-07-13 2009-01-13 Marc-Andre D'aoust Influenza virus-like particles (vlps) comprising hemagglutinin
WO2009076778A1 (en) 2007-11-27 2009-06-25 Medicago Inc. Recombinant influenza virus-like particles (vlps) produced in transgenic plants expressing hemagglutinin
HUE039100T2 (hu) 2009-06-24 2018-12-28 Medicago Inc Hemagglutinint tartalmazó kimer influenzavírus-jellegû részecskék
KR101773431B1 (ko) * 2009-09-22 2017-09-12 메디카고 인코포레이티드 식물-유래 vlp의 제조방법
TWI526539B (zh) 2010-12-22 2016-03-21 苜蓿股份有限公司 植物中生產類病毒顆粒(vlp)的方法及以該方法生產之vlp
TWI620816B (zh) 2011-03-23 2018-04-11 苜蓿股份有限公司 植物衍生蛋白回收方法
KR101963792B1 (ko) 2011-06-13 2019-07-31 메디카고 인코포레이티드 식물에서 광견병 바이러스 유사 입자 생성
EP2758038B1 (en) * 2011-09-23 2018-05-30 The United States of America, as represented by The Secretary, Department of Health & Human Services Novel influenza hemagglutinin protein-based vaccines
US11390878B2 (en) 2011-09-30 2022-07-19 Medicago Inc. Increasing protein yield in plants
NZ622731A (en) 2011-09-30 2016-04-29 Medicago Inc Increasing virus-like particle yield in plants
EP2798059B1 (en) * 2011-12-21 2021-09-29 Apse, Llc Process for purifying vlps with capsids resistant to hydrolases
JP6406655B2 (ja) * 2012-02-22 2018-10-17 三菱商事フードテック株式会社 植物性食品素材の軟化方法、軟化製剤、軟化した植物性食品素材およびそれを用いた食品
ES2657875T3 (es) * 2012-03-22 2018-03-07 Fraunhofer Usa Inc. Partículas tipo virus que comprenden una proteína de la matriz de un virus encapsulado de plantas y usos de las mismas
JP2015528285A (ja) 2012-09-05 2015-09-28 メディカゴ インコーポレイテッド 植物におけるピコルナウイルス様粒子の産生
US9617297B2 (en) * 2012-10-11 2017-04-11 The Regents Of The University Of California Apoplast wash fluid recovery for improved recombinant endoglucanase extraction in tabacco leaves
US12467058B2 (en) 2013-03-28 2025-11-11 Aramis Biotechnologies Inc. Influenza virus-like particle production in plants
ES2803508T3 (es) * 2013-03-28 2021-01-27 Medicago Inc Producción de partículas similares al virus de la gripe en plantas
JP6358257B2 (ja) * 2013-09-06 2018-07-18 三菱ケミカル株式会社 植物を用いたタンパク質の製造方法
WO2015054639A1 (en) 2013-10-11 2015-04-16 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Epstein-barr virus vaccines
RU2699982C2 (ru) 2014-01-10 2019-09-11 Медикаго Инк. Энхансерные элементы cpmv
RU2731295C2 (ru) 2014-07-11 2020-09-01 Медикаго Инк. Модификация продуцирования белков в растениях
US11191727B2 (en) 2014-12-31 2021-12-07 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Multivalent nanoparticle-based vaccines
JP2019129705A (ja) * 2016-03-31 2019-08-08 日本たばこ産業株式会社 植物に物質を導入する方法
SG10201912944QA (en) 2016-09-02 2020-02-27 The Usa As Represented By The Secretary Stabilized group 2 influenza hemagglutinin stem region trimers and uses thereof
WO2019104439A1 (en) * 2017-11-30 2019-06-06 Medicago Inc. Modified norovirus vp1 proteins and vlps comprising modified norovirus vp1 proteins
EP3814509A4 (en) 2018-06-27 2022-07-27 Medicago Inc. INFLUENCE VIRUS HEMAGGLUTININ MUTANTS
CA3106471A1 (en) * 2018-07-13 2020-01-16 Medicago Inc. Modified norovirus vp1 proteins and vlps comprising modified norovirus vp1 proteins
CN109112093B (zh) * 2018-09-05 2021-11-09 上海交通大学 一种青蒿原生质体高效分离及瞬时转化的方法
CA3137447A1 (en) * 2019-04-25 2020-10-29 Flagship Pioneering Innovations Vi, Llc Compositions and methods relating to plant messenger packs
CN110483616B (zh) * 2019-07-31 2021-06-04 中国农业科学院农产品加工研究所 从感染病原菌的植物组织中分离病原菌分泌的质外体效应蛋白的方法
CN111060696B (zh) * 2019-12-27 2023-09-08 湖南农业大学 一种降低植物小分子信号肽假阳性率的方法
CN111718394A (zh) * 2020-06-28 2020-09-29 广西壮族自治区农业科学院 一种基于bpp法的甘蔗组织变性蛋白提取方法
CN111826394A (zh) * 2020-08-07 2020-10-27 浙江华缔药业集团医药开发有限公司 植物瞬时表达载体的构建及应用
WO2022260849A1 (en) 2021-06-09 2022-12-15 Nant Holdings Ip, Llc Methods and systems for producing a protein of interest in a plant
CN114934007B (zh) * 2022-07-01 2023-11-24 广东省农业科学院作物研究所 一种甘薯原生质体的制备方法
JP7745040B1 (ja) 2024-06-21 2025-09-26 デンカ株式会社 目的タンパク質の回収方法及び製造方法
CN120082594B (zh) * 2025-04-07 2025-10-28 贵州大学 NbClpT2在调控植物抗PVY侵染中的应用

Family Cites Families (77)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4945050A (en) 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
US5100792A (en) 1984-11-13 1992-03-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues
US5036006A (en) 1984-11-13 1991-07-30 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
EP0203177A4 (en) 1984-11-29 1987-04-28 Scripps Clinic Res POLYPEPTIDES AND ANTIBODIES ASSOCIATED WITH VIRAL GLUCOPROTEINS WITHOUT GLUCOSE.
JPS61265086A (ja) * 1985-05-21 1986-11-22 Mitsui Toatsu Chem Inc プロトプラストの培養法
US5232833A (en) 1988-09-14 1993-08-03 Stressgen Biotechnologies Corporation Accumulation of heat shock proteins for evaluating biological damage due to chronic exposure of an organism to sublethal levels of pollutants
US6403865B1 (en) 1990-08-24 2002-06-11 Syngenta Investment Corp. Method of producing transgenic maize using direct transformation of commercially important genotypes
ATE142883T1 (de) 1991-03-28 1996-10-15 Rooperol Na Nv Zusammensetzungen von phytosterolen mit phytosterolinen als immunmodulatoren
UA48104C2 (uk) 1991-10-04 2002-08-15 Новартіс Аг Фрагмент днк, який містить послідовність,що кодує інсектицидний протеїн, оптимізовану для кукурудзи,фрагмент днк, який забезпечує направлену бажану для серцевини стебла експресію зв'язаного з нею структурного гена в рослині, фрагмент днк, який забезпечує специфічну для пилку експресію зв`язаного з нею структурного гена в рослині, рекомбінантна молекула днк, спосіб одержання оптимізованої для кукурудзи кодуючої послідовності інсектицидного протеїну, спосіб захисту рослин кукурудзи щонайменше від однієї комахи-шкідника
US6326470B1 (en) 1997-04-15 2001-12-04 The Penn State Research Foundation Enhancement of accessibility of cellulose by expansins
US5762939A (en) 1993-09-13 1998-06-09 Mg-Pmc, Llc Method for producing influenza hemagglutinin multivalent vaccines using baculovirus
GB9414118D0 (en) 1994-07-13 1994-08-31 Axis Genetics Ltd Modified plant viruses as vectors of heterologous peptides
AU6504796A (en) 1995-07-20 1997-02-18 Washington State University Research Foundation Production of secreted foreign polypeptides in plant cell culture
US5773695A (en) * 1996-01-26 1998-06-30 North Carolina State University Plant nuclear scaffold attachment region and method for increasing gene expression in transgenic cells
US6042832A (en) 1996-08-28 2000-03-28 Thomas Jefferson University Polypeptides fused with alfalfa mosaic virus or ilarvirus capsid proteins
US20010006950A1 (en) 1998-02-11 2001-07-05 Juha Punnonen Genetic vaccine vector engineering
US6489537B1 (en) 1998-08-07 2002-12-03 The Trustees Of The University Of Pennsylvania Phytochelatin synthases and uses therefor
IL141312A0 (en) 1998-08-11 2002-03-10 Biosource Tech Inc Method for recovering proteins from the interstitial fluid of plant tissues
US6287570B1 (en) 1998-11-23 2001-09-11 Patricia L. Foley Vaccine against swine influenza virus
JP2002533090A (ja) 1998-12-23 2002-10-08 ザ、サミュアル、ラバツ、ノゥブル、ファウンデイシャン、インク 植物形質転換法
FR2791358B1 (fr) 1999-03-22 2003-05-16 Meristem Therapeutics Promoteurs chimeriques d'expression, cassettes d'expression, plasmides, vecteurs, plantes et semences transgeniques les contenant et leurs methodes d'obtention
WO2000063400A2 (en) 1999-04-21 2000-10-26 The Samuel Roberts Noble Foundation Plant transformation process
US7125978B1 (en) 1999-10-04 2006-10-24 Medicago Inc. Promoter for regulating expression of foreign genes
WO2002012454A1 (en) * 2000-08-08 2002-02-14 The Kitasato Institute Virus-like micrograins and process for producing the same
CA2435000C (en) 2001-01-18 2014-03-25 Vlaams Interuniversitair Instituut Voor Biotechnologie Vzw Oligomeric complexes of chimeric proteins with enhanced immunogenic potential
US20050037420A1 (en) 2001-09-14 2005-02-17 Mei-Yun Zhang Immunoglobulin having particular framework scaffold and methods of making and using
KR101113432B1 (ko) 2002-02-13 2012-02-29 위스콘신 얼럼나이 리서어치 화운데이션 인플루엔자 바이러스 벡터의 패키징을 위한 신호
US20040121465A1 (en) 2002-02-14 2004-06-24 Novavax, Inc. Optimization of gene sequences of virus-like particles for expression in insect cells
CN1665934B (zh) 2002-03-19 2010-05-26 国际植物研究所 GNTIII(UDP-N乙酰葡萄糖胺:β-D-甘露糖苷β(1,4)-N-乙酰葡萄糖胺基-转移酶III)在植物中的表达
BRPI0410342A (pt) 2003-05-05 2006-06-20 Dow Agrosciences Llc composições imunoprofiláticas e terapêuticas estáveis derivadas de células vegetais transgênicas e processos para produção
KR20060035596A (ko) * 2003-05-05 2006-04-26 보이스 톰슨 인스터튜트 포 플랜트 리서치 외래유전자도입 식물로부터 유래된 면역보호성 조성물을제조하기 위한 벡터와 세포
US7566458B2 (en) 2003-06-16 2009-07-28 Medimmune, Llc Influenza hemagglutinin and neuraminidase variants
US8592197B2 (en) 2003-07-11 2013-11-26 Novavax, Inc. Functional influenza virus-like particles (VLPs)
CN1560227A (zh) * 2004-02-25 2005-01-05 福建师范大学 雨生红球藻原生质体制备与再生技术
WO2006016380A2 (en) 2004-08-13 2006-02-16 Council Of Scientific And Industrial Research A chimeric g protein based rabies vaccine
US9155483B2 (en) 2004-12-03 2015-10-13 The Invention Science Fund I, Llc Vision modification with reflected image
CA2611859C (en) * 2005-03-15 2015-03-31 Verenium Corporation Cellulases, nucleic acids encoding them and methods for making and using them
ES2662118T3 (es) 2005-04-29 2018-04-05 University Of Cape Town Expresión de proteínas virales en las plantas
CN100410378C (zh) 2005-05-09 2008-08-13 中国农业科学院生物技术研究所 编码禽流感血凝素的基因及其植物表达载体和应用
CA2613160A1 (en) * 2005-06-24 2006-12-28 Bayer Bioscience N.V. Methods for altering the reactivity of plant cell walls
CA2615658A1 (en) 2005-07-19 2007-01-25 Dow Global Technolgies Inc. Recombinant flu vaccines
US7871626B2 (en) 2005-08-04 2011-01-18 St. Jude Children's Research Hospital Modified influenza virus for monitoring and improving vaccine efficiency
KR101359723B1 (ko) 2005-10-18 2014-02-05 노바백스, 인코포레이티드 기능성 인플루엔자 바이러스 유사 입자들
ES2514316T3 (es) * 2005-11-22 2014-10-28 Novartis Vaccines And Diagnostics, Inc. Partículas similares a virus (VLPs) de Norovirus y Sapovirus
BRPI0707733B1 (pt) 2006-02-13 2019-12-31 Ibio, Inc. antígeno isolado, composição de vacina, uso da referida composição e método para produção de uma proteína de antígeno
AR060565A1 (es) 2006-04-21 2008-06-25 Dow Agrosciences Llc Vacuna para la gripe aviaria y metodos de uso
WO2007130327A2 (en) 2006-05-01 2007-11-15 Technovax, Inc. Influenza virus-like particle (vlp) compositions
WO2008054540A2 (en) 2006-05-18 2008-05-08 Pharmexa Inc. Inducing immune responses to influenza virus using polypeptide and nucleic acid compositions
CA2651907C (en) 2006-05-22 2016-12-20 Plant Bioscience Limited Bipartite system, method and composition for the constitutive and inducible expression of high levels of foreign proteins in plants
US20070286873A1 (en) 2006-05-23 2007-12-13 Williams John V Recombinant Influenza H5 Hemagluttinin Protein And Nucleic Acid Coding Therefor
EP2120762A1 (en) 2007-01-17 2009-11-25 Lerner Medical Devices, Inc. Fiber optic phototherapy device
US7730950B2 (en) 2007-01-19 2010-06-08 Halliburton Energy Services, Inc. Methods for treating intervals of a subterranean formation having variable permeability
DK2155880T3 (en) 2007-06-15 2016-12-05 Medicago Inc Modification of glycoproteinproduktion i plants
KR100964462B1 (ko) 2007-07-10 2010-06-16 성균관대학교산학협력단 형질전환 식물 유래의 조류독감 바이러스 백신 및 그 제조방법
WO2009076778A1 (en) 2007-11-27 2009-06-25 Medicago Inc. Recombinant influenza virus-like particles (vlps) produced in transgenic plants expressing hemagglutinin
CA2615372A1 (en) 2007-07-13 2009-01-13 Marc-Andre D'aoust Influenza virus-like particles (vlps) comprising hemagglutinin
RU2358981C2 (ru) * 2007-08-07 2009-06-20 Центр "Биоинженерия" Российской Академии Наук Универсальная вакцина против вируса гриппа птиц
WO2009026397A2 (en) 2007-08-20 2009-02-26 Fraunhofer Usa, Inc. Prophylactic and therapeutic influenza vaccines, antigens, compositions, and methods
CN101422128B (zh) * 2007-10-29 2011-06-08 中国水产科学研究院黄海水产研究所 马尾藻原生质体的分离和再生
WO2009083420A1 (en) * 2007-12-28 2009-07-09 Unilever Plc Process for recovering aroma from tea
US8799801B2 (en) 2008-01-16 2014-08-05 Qualcomm Incorporated Interactive ticker
SG187500A1 (en) 2008-01-21 2013-02-28 Medicago Inc Recombinant influenza virus-like particles (vlps) produced in transgenic plants expressing hemagglutinin
AU2009267769B2 (en) 2008-07-08 2015-07-16 Medicago Inc. Soluble recombinant influenza antigens
CN101626276A (zh) 2008-07-10 2010-01-13 华为技术有限公司 广告替换方法、装置及系统
NZ590351A (en) 2008-07-18 2012-11-30 Medicago Inc HA1 domain totally or partially free of N-linked glycosylation operatively linked to a regulatory region
CA2736796A1 (en) 2008-08-27 2010-03-04 Arizona Board Of Regents For And On Behalf Of Arizona State University A dna replicon system for high-level rapid production of vaccines and monoclonal antibody therapeutics in plants
WO2010042551A2 (en) * 2008-10-06 2010-04-15 Genvault Corporation Methods for providing cellular lysates from cell wall-containing samples
WO2010077712A1 (en) 2008-12-09 2010-07-08 Novavax, Inc. Bovine respiratory syncytial virus virus-like particle (vlps)
CN102365295A (zh) 2009-01-30 2012-02-29 阿德利夫股份有限公司 构象动力肽
CA3018494C (en) 2009-06-10 2021-12-07 Nono Inc. Co-administration of an agent linked to an internalization peptide with an anti-inflammatory
KR101766408B1 (ko) 2009-06-10 2017-08-10 알닐람 파마슈티칼스 인코포레이티드 향상된 지질 조성물
HUE039100T2 (hu) 2009-06-24 2018-12-28 Medicago Inc Hemagglutinint tartalmazó kimer influenzavírus-jellegû részecskék
KR101773431B1 (ko) 2009-09-22 2017-09-12 메디카고 인코포레이티드 식물-유래 vlp의 제조방법
WO2012058762A1 (en) 2010-11-04 2012-05-10 Medicago Inc. Plant expression system
KR101847908B1 (ko) 2010-11-05 2018-04-11 노바백스, 인코포레이티드 광견병 당단백질 바이러스-유사 입자(VLPs)
TWI526539B (zh) 2010-12-22 2016-03-21 苜蓿股份有限公司 植物中生產類病毒顆粒(vlp)的方法及以該方法生產之vlp
TWI620816B (zh) * 2011-03-23 2018-04-11 苜蓿股份有限公司 植物衍生蛋白回收方法

Similar Documents

Publication Publication Date Title
JP2013505025A5 (https=)
RU2012115661A (ru) Способ получения вирусоподобных частиц растений
TN2009000557A1 (en) Influenza virus-like particles (vlps) comprising hemagglutinin produced within a plant
CN108070032B (zh) 一种重组人源胶原蛋白的纯化方法
WO2012171026A3 (en) Methods for high yield virus production
NZ602248A (en) Purified mesenchymal stem cell compositions and methods of purifying mesenchymal stem cell compositions
CN103073652A (zh) 一种螺旋藻多糖的提取方法
CN102304168A (zh) 小球藻蛋白质的低温超高压制备方法
ZA202100384B (en) Influenza virus hemagglutinin mutants
CN105061633A (zh) 一种利用马尾藻制取褐藻糖胶的方法
CN105272887A (zh) 一种从鲍鱼内脏中同时提取牛磺酸和多糖的方法
CN102382203A (zh) 一种莲子多糖高效提取工艺
CN103740686B (zh) 一种用醋酸提取激肽释放酶的方法
CN103483461A (zh) 唇香草多糖提取物及其制备方法
CN104479040A (zh) 一种竹荪多糖的提取工艺
CN101619099B (zh) 反胶束提取菜籽蛋白的方法
CN103642760B (zh) 一种制备柯萨奇a16型病毒完整实心颗粒的方法
CN103289969A (zh) 一种从植物茎叶中提取超氧化物歧化酶的方法
CN102584936A (zh) 一种采用膜法生产云芝糖肽的加工方法
CN106674538A (zh) 一种从竹材中分离提取纤维素、半纤维素降解糖和木质素的方法
KR102387853B1 (ko) 젖은 미세조류로부터 바이오디젤의 제조방법
CN103923131A (zh) 利用啤酒废酵母制备海藻糖和食品增鲜剂的方法
CN102286069B (zh) 一种有机溶剂沉淀分离初步提纯谷胱甘肽发酵液的方法
CN102827244A (zh) 一种精制谷胱甘肽发酵液的方法
CN103757071A (zh) 一种生产酵母聚糖的方法