JP2012517816A5 - - Google Patents

Info

Publication number

JP2012517816A5

JP2012517816A5 JP2011550531A JP2011550531A JP2012517816A5 JP 2012517816 A5 JP2012517816 A5 JP 2012517816A5 JP 2011550531 A JP2011550531 A JP 2011550531A JP 2011550531 A JP2011550531 A JP 2011550531A JP 2012517816 A5 JP2012517816 A5 JP 2012517816A5

Authority

JP

Japan

Prior art keywords

mutated

dehydrogenase

analyte

amino acid

glucose dehydrogenase

Prior art date

2010-02-12

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Granted

Links

• Espacenet
• Global Dossier
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• 101710088194 Dehydrogenase Proteins 0.000 claims description 8
• 230000035772 mutation Effects 0.000 claims description 7
• 238000006467 substitution reaction Methods 0.000 claims description 7
• KJBQDYDTQCIAFO-ZLELNMGESA-N (2s)-2-amino-4-methylpentanoic acid;(2s)-2,6-diaminohexanoic acid Chemical compound CC(C)C[C@H](N)C(O)=O.NCCCC[C@H](N)C(O)=O KJBQDYDTQCIAFO-ZLELNMGESA-N 0.000 claims description 2
• HOMROMWVNDUGRI-RVZXSAGBSA-N (2s)-2-aminopentanedioic acid;(2s)-2,6-diaminohexanoic acid Chemical compound NCCCC[C@H](N)C(O)=O.OC(=O)[C@@H](N)CCC(O)=O HOMROMWVNDUGRI-RVZXSAGBSA-N 0.000 claims description 2
• 239000004475 Arginine Substances 0.000 claims description 2
• ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 claims description 2
• KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 claims description 2
• 239000004472 Lysine Substances 0.000 claims description 2
• ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 claims description 2
• 238000000034 method Methods 0.000 claims 15
• 108010050375 Glucose 1-Dehydrogenase Proteins 0.000 claims 9
• 239000012491 analyte Substances 0.000 claims 9
• 239000005515 coenzyme Substances 0.000 claims 7
• XJLXINKUBYWONI-DQQFMEOOSA-N [[(2r,3r,4r,5r)-5-(6-aminopurin-9-yl)-3-hydroxy-4-phosphonooxyoxolan-2-yl]methoxy-hydroxyphosphoryl] [(2s,3r,4s,5s)-5-(3-carbamoylpyridin-1-ium-1-yl)-3,4-dihydroxyoxolan-2-yl]methyl phosphate Chemical compound NC(=O)C1=CC=C[N+]([C@@H]2[C@H]([C@@H](O)[C@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](OP(O)(O)=O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 XJLXINKUBYWONI-DQQFMEOOSA-N 0.000 claims 6
• 125000003275 alpha amino acid group Chemical group 0.000 claims 6
• 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 claims 6
• BAWFJGJZGIEFAR-NNYOXOHSSA-N NAD zwitterion Chemical compound NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 BAWFJGJZGIEFAR-NNYOXOHSSA-N 0.000 claims 4
• 150000001875 compounds Chemical class 0.000 claims 4
• 229950006238 nadide Drugs 0.000 claims 4
• 244000063299 Bacillus subtilis Species 0.000 claims 2
• 235000014469 Bacillus subtilis Nutrition 0.000 claims 2
• 239000003153 chemical reaction reagent Substances 0.000 claims 2
• 125000004122 cyclic group Chemical group 0.000 claims 2
• 230000001419 dependent effect Effects 0.000 claims 2
• BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 claims 2
• 229910052760 oxygen Inorganic materials 0.000 claims 2
• 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 claims 2
• 229910052717 sulfur Inorganic materials 0.000 claims 2
• 230000007306 turnover Effects 0.000 claims 2
• GVNVAWHJIKLAGL-UHFFFAOYSA-N 2-(cyclohexen-1-yl)cyclohexan-1-one Chemical compound O=C1CCCCC1C1=CCCCC1 GVNVAWHJIKLAGL-UHFFFAOYSA-N 0.000 claims 1
• 229930024421 Adenine Natural products 0.000 claims 1
• GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 claims 1
• 241000194107 Bacillus megaterium Species 0.000 claims 1
• 241000193388 Bacillus thuringiensis Species 0.000 claims 1
• 101150065749 Churc1 gene Proteins 0.000 claims 1
• 108090000790 Enzymes Proteins 0.000 claims 1
• 102000004190 Enzymes Human genes 0.000 claims 1
• 102100038239 Protein Churchill Human genes 0.000 claims 1
• JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical group C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 claims 1
• 229960000643 adenine Drugs 0.000 claims 1
• 229940097012 bacillus thuringiensis Drugs 0.000 claims 1
• 230000000694 effects Effects 0.000 claims 1
• 125000002887 hydroxy group Chemical group [H]O* 0.000 claims 1
• 150000003839 salts Chemical class 0.000 claims 1
• 235000001014 amino acid Nutrition 0.000 description 4
• 150000001413 amino acids Chemical group 0.000 description 4
• WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 1
• 235000013922 glutamic acid Nutrition 0.000 description 1
• 239000004220 glutamic acid Substances 0.000 description 1
• 230000003301 hydrolyzing effect Effects 0.000 description 1
• 230000006641 stabilisation Effects 0.000 description 1
• 238000011105 stabilization Methods 0.000 description 1