CA2721718C - Stabilization of dehydrogenases with stable coenzymes - Google Patents

Stabilization of dehydrogenases with stable coenzymes Download PDF

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Publication number
CA2721718C
CA2721718C CA2721718A CA2721718A CA2721718C CA 2721718 C CA2721718 C CA 2721718C CA 2721718 A CA2721718 A CA 2721718A CA 2721718 A CA2721718 A CA 2721718A CA 2721718 C CA2721718 C CA 2721718C
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CA
Canada
Prior art keywords
enzyme
dehydrogenase
case independently
nad
stabilized
Prior art date
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Expired - Fee Related
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CA2721718A
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English (en)
French (fr)
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CA2721718A1 (en
Inventor
Dieter Heindl
Carina Horn
Claudia Gaessler-Dietsche
Joachim Hoenes
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F Hoffmann La Roche AG
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F Hoffmann La Roche AG
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Publication of CA2721718A1 publication Critical patent/CA2721718A1/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0014—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4)
    • C12N9/0016—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4) with NAD or NADP as acceptor (1.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01001—Alcohol dehydrogenase (1.1.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01006—Glycerol dehydrogenase (1.1.1.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01027—L-Lactate dehydrogenase (1.1.1.27)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01028—D-Lactate dehydrogenase (1.1.1.28)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01037—Malate dehydrogenase (1.1.1.37)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01047—Glucose 1-dehydrogenase (1.1.1.47)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y104/00—Oxidoreductases acting on the CH-NH2 group of donors (1.4)
    • C12Y104/01—Oxidoreductases acting on the CH-NH2 group of donors (1.4) with NAD+ or NADP+ as acceptor (1.4.1)
    • C12Y104/01005—L-Amino-acid dehydrogenase (1.4.1.5)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CA2721718A 2008-02-19 2009-02-19 Stabilization of dehydrogenases with stable coenzymes Expired - Fee Related CA2721718C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP08003054.7 2008-02-19
EP08003054A EP2093284A1 (de) 2008-02-19 2008-02-19 Stabilisierung von Dehydrogenasen mit stabilen Coenzymen
PCT/EP2009/001206 WO2009103540A1 (de) 2008-02-19 2009-02-19 Stabilisierung von dehydrogenasen mit stabilen coenzymen

Publications (2)

Publication Number Publication Date
CA2721718A1 CA2721718A1 (en) 2009-08-27
CA2721718C true CA2721718C (en) 2017-11-28

Family

ID=39768713

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2721718A Expired - Fee Related CA2721718C (en) 2008-02-19 2009-02-19 Stabilization of dehydrogenases with stable coenzymes

Country Status (15)

Country Link
US (2) US9896666B2 (enExample)
EP (2) EP2093284A1 (enExample)
JP (2) JP5758128B2 (enExample)
KR (3) KR20130081300A (enExample)
CN (3) CN101945999B (enExample)
AR (1) AR070436A1 (enExample)
AU (1) AU2009216908B9 (enExample)
BR (1) BRPI0907839A2 (enExample)
CA (1) CA2721718C (enExample)
HK (1) HK1208703A1 (enExample)
MX (1) MX2010008920A (enExample)
RU (1) RU2499834C2 (enExample)
TW (1) TWI482860B (enExample)
WO (1) WO2009103540A1 (enExample)
ZA (1) ZA201006638B (enExample)

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EP2226007A1 (de) 2009-02-19 2010-09-08 Roche Diagnostics GmbH Testelementmagazin mit abgedeckten Testfeldern
EP2226008A1 (de) 2009-02-19 2010-09-08 Roche Diagnostics GmbH Verfahren zur Herstellung eines analytischen Magazins
CA2750474C (en) * 2009-02-19 2017-07-25 F. Hoffmann-La Roche Ag Fast reaction kinetics of enzymes having low activity in dry chemistry layers
CN102325496B (zh) 2009-02-19 2015-10-07 霍夫曼-拉罗奇有限公司 分析辅助工具的节省空间的储存
EP2398909B1 (de) 2009-02-19 2015-07-22 F. Hoffmann-La Roche AG Schnelle reaktionskinetik von enzymen mit niedriger aktivität in trockenen chemieschichten
EP2292751A1 (de) * 2009-08-20 2011-03-09 Roche Diagnostics GmbH Stabilisierung von Enzymen mit stabilen Coenzymen
EP2513648B1 (de) * 2009-12-16 2015-05-27 F.Hoffmann-La Roche Ag Detektion der zersetzung von enzymen in einem testelement durch kontrollierte freisetzung von geschütztem analyt
EP2636750A1 (en) * 2012-03-06 2013-09-11 Roche Diagniostics GmbH Compatible solute ectoine as well as derivatives thereof for enzyme stabilization
EP2864765B1 (en) 2012-06-22 2021-04-28 Roche Diabetes Care GmbH Method and device for detecting an analyte in a body fluid
US8920628B2 (en) 2012-11-02 2014-12-30 Roche Diagnostics Operations, Inc. Systems and methods for multiple analyte analysis
US8921061B2 (en) * 2012-11-02 2014-12-30 Roche Diagnostics Operations, Inc. Reagent materials and associated test elements
EP2936155B1 (en) 2012-12-20 2018-12-12 Roche Diabetes Care GmbH Method for analyzing a sample of a body fluid
KR101789893B1 (ko) 2012-12-20 2017-11-20 에프. 호프만-라 로슈 아게 의학적 측정 곡선들을 평가하기 위한 방법
EP2781919A1 (en) 2013-03-19 2014-09-24 Roche Diagniostics GmbH Method / device for generating a corrected value of an analyte concentration in a sample of a body fluid
EP2994536B1 (en) 2013-05-08 2017-06-14 Roche Diabetes Care GmbH Stabilization of enzymes by nicotinic acid
EP3004372A1 (en) * 2013-06-04 2016-04-13 Roche Diagnostics GmbH Novel compounds useful for fret and methods related thereto
WO2015063025A1 (en) 2013-10-29 2015-05-07 F. Hoffmann-La Roche Ag Nano-enzyme containers for test elements
WO2015078899A1 (en) 2013-11-27 2015-06-04 Roche Diagnostics Gmbh Composition comprising up-converting phosphors for detecting an analyte
EP2927319A1 (en) 2014-03-31 2015-10-07 Roche Diagnostics GmbH High load enzyme immobilization by crosslinking
CN106164054B (zh) 2014-04-14 2019-05-07 豪夫迈·罗氏有限公司 吩嗪介导剂
EP3183246B1 (en) 2014-08-22 2020-09-23 Roche Diagnostics GmbH Redoxindicators
CN107779459B (zh) * 2016-08-31 2021-06-08 安琪酵母股份有限公司 葡萄糖脱氢酶dna分子、载体和菌株及应用
JP2019529935A (ja) 2016-10-05 2019-10-17 エフ ホフマン−ラ ロッシュ アクチェン ゲゼルシャフト 多検体診断用試験エレメントのための検出試薬および電極配置、ならびにそれらを使用する方法
EP3339431A1 (en) 2016-12-22 2018-06-27 Roche Diabetes Care GmbH Glucose dehydrogenase variants with improved properties
HUE064713T2 (hu) 2018-02-28 2024-04-28 Hoffmann La Roche Biokompatibilitási bevonat folyamatos analitméréshez
CN122235370A (zh) * 2026-05-20 2026-06-19 山东农业大学 一种与苹果果实山梨醇含量相关的分子标记、检测试剂及其应用

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Also Published As

Publication number Publication date
EP2245152B1 (de) 2019-05-01
AU2009216908A1 (en) 2009-08-27
CA2721718A1 (en) 2009-08-27
US11220674B2 (en) 2022-01-11
US20110143416A1 (en) 2011-06-16
AR070436A1 (es) 2010-04-07
KR20100118995A (ko) 2010-11-08
US9896666B2 (en) 2018-02-20
CN101945999A (zh) 2011-01-12
CN104450659A (zh) 2015-03-25
EP2245152A1 (de) 2010-11-03
TWI482860B (zh) 2015-05-01
JP6113405B2 (ja) 2017-04-12
KR20120134142A (ko) 2012-12-11
BRPI0907839A2 (pt) 2015-08-11
AU2009216908B9 (en) 2012-07-05
CN101945999B (zh) 2016-06-08
KR101381004B1 (ko) 2014-04-08
HK1208703A1 (en) 2016-03-11
RU2499834C2 (ru) 2013-11-27
CN102325896A (zh) 2012-01-18
KR20130081300A (ko) 2013-07-16
EP2093284A1 (de) 2009-08-26
RU2010138610A (ru) 2012-03-27
MX2010008920A (es) 2010-09-07
JP5758128B2 (ja) 2015-08-05
WO2009103540A1 (de) 2009-08-27
AU2009216908B2 (en) 2012-04-19
US20180265849A1 (en) 2018-09-20
TW200951222A (en) 2009-12-16
ZA201006638B (en) 2011-06-29
JP2011514153A (ja) 2011-05-06
JP2012517816A (ja) 2012-08-09

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