AR070436A1 - Estabilizacion de deshidrogenasas con coenzimas estables - Google Patents

Estabilizacion de deshidrogenasas con coenzimas estables

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Publication number
AR070436A1
AR070436A1 ARP090100571A ARP090100571A AR070436A1 AR 070436 A1 AR070436 A1 AR 070436A1 AR P090100571 A ARP090100571 A AR P090100571A AR P090100571 A ARP090100571 A AR P090100571A AR 070436 A1 AR070436 A1 AR 070436A1
Authority
AR
Argentina
Prior art keywords
case independently
stable
enzyme
stable coenzyme
stabilization
Prior art date
Application number
ARP090100571A
Other languages
English (en)
Inventor
Dieter Heindl
Carina Horn
Joachim Hoenes
Claudia Gaessler-Dietsche
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of AR070436A1 publication Critical patent/AR070436A1/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0014—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4)
    • C12N9/0016—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4) with NAD or NADP as acceptor (1.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01001—Alcohol dehydrogenase (1.1.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01006—Glycerol dehydrogenase (1.1.1.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01027—L-Lactate dehydrogenase (1.1.1.27)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01028—D-Lactate dehydrogenase (1.1.1.28)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01037—Malate dehydrogenase (1.1.1.37)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01047—Glucose 1-dehydrogenase (1.1.1.47)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y104/00—Oxidoreductases acting on the CH-NH2 group of donors (1.4)
    • C12Y104/01—Oxidoreductases acting on the CH-NH2 group of donors (1.4) with NAD+ or NADP+ as acceptor (1.4.1)
    • C12Y104/01005—L-Amino-acid dehydrogenase (1.4.1.5)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Procedimiento para la estabilizacion de una enzima deshidrogenasa por almacenamiento de la enzima en presencia de una coenzima estable. Además una enzima estabilizada con una coenzima estable así como también a su uso en elementos de prueba para la determinacion de substancias a analizar. Reivindicacion 5: Procedimiento de acuerdo con una de las reivindicaciones 1 a 4, caracterizado porque la coenzima estable es seleccionada entre compuestos de nicotinamida-adenina-dinucleotido (NAD/NADH) y nicotinamida-adenina-dinucleotido fosfato (NADP/NADPH) estables y el compuesto de la Formula (1). Reivindicacion 6: Procedimiento de acuerdo con la reivindicacion 5, caracterizado porque la coenzima estable es seleccionada entre los compuestos con la formula general (2), con: A = adenina o un análogo de la misma, T = en cada caso independientemente O, S; U = en cada caso independientemente OH, SH, BH3-, BCNH2-; V = en cada caso independientemente OH o un grupo fosfato, o dos grupos que forman un grupo fosfato cíclico; W = COOR, CON(R)2, COR, CSN(R)2 con R = en cada caso independientemente H o alquilo C1-2; X1, X2 = en cada caso independientemente O, CH2, CHCH3, C(CH3)2, NH, NCH3; Y = NH, S, O, CH2; Z = es un resto orgánico lineal o cíclico, con la condicion de que Z y el resto piridina no estén unidos por una union glicosídica, o una sal o eventualmente una forma reducida del mismo.
ARP090100571A 2008-02-19 2009-02-19 Estabilizacion de deshidrogenasas con coenzimas estables AR070436A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP08003054A EP2093284A1 (de) 2008-02-19 2008-02-19 Stabilisierung von Dehydrogenasen mit stabilen Coenzymen

Publications (1)

Publication Number Publication Date
AR070436A1 true AR070436A1 (es) 2010-04-07

Family

ID=39768713

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP090100571A AR070436A1 (es) 2008-02-19 2009-02-19 Estabilizacion de deshidrogenasas con coenzimas estables

Country Status (15)

Country Link
US (2) US9896666B2 (es)
EP (2) EP2093284A1 (es)
JP (2) JP5758128B2 (es)
KR (3) KR20120134142A (es)
CN (3) CN101945999B (es)
AR (1) AR070436A1 (es)
AU (1) AU2009216908B9 (es)
BR (1) BRPI0907839A2 (es)
CA (1) CA2721718C (es)
HK (1) HK1208703A1 (es)
MX (1) MX2010008920A (es)
RU (1) RU2499834C2 (es)
TW (1) TWI482860B (es)
WO (1) WO2009103540A1 (es)
ZA (1) ZA201006638B (es)

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EP2093284A1 (de) 2008-02-19 2009-08-26 F.Hoffmann-La Roche Ag Stabilisierung von Dehydrogenasen mit stabilen Coenzymen
EP2398909B1 (de) 2009-02-19 2015-07-22 F. Hoffmann-La Roche AG Schnelle reaktionskinetik von enzymen mit niedriger aktivität in trockenen chemieschichten
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Also Published As

Publication number Publication date
KR20120134142A (ko) 2012-12-11
EP2093284A1 (de) 2009-08-26
HK1208703A1 (en) 2016-03-11
JP2011514153A (ja) 2011-05-06
CN102325896A (zh) 2012-01-18
EP2245152B1 (de) 2019-05-01
CN101945999A (zh) 2011-01-12
JP2012517816A (ja) 2012-08-09
CN104450659A (zh) 2015-03-25
KR20100118995A (ko) 2010-11-08
TWI482860B (zh) 2015-05-01
RU2499834C2 (ru) 2013-11-27
JP6113405B2 (ja) 2017-04-12
AU2009216908A1 (en) 2009-08-27
WO2009103540A1 (de) 2009-08-27
KR101381004B1 (ko) 2014-04-08
CA2721718A1 (en) 2009-08-27
EP2245152A1 (de) 2010-11-03
US20180265849A1 (en) 2018-09-20
MX2010008920A (es) 2010-09-07
TW200951222A (en) 2009-12-16
KR20130081300A (ko) 2013-07-16
US11220674B2 (en) 2022-01-11
BRPI0907839A2 (pt) 2015-08-11
CN101945999B (zh) 2016-06-08
JP5758128B2 (ja) 2015-08-05
AU2009216908B9 (en) 2012-07-05
ZA201006638B (en) 2011-06-29
US9896666B2 (en) 2018-02-20
RU2010138610A (ru) 2012-03-27
US20110143416A1 (en) 2011-06-16
CA2721718C (en) 2017-11-28
AU2009216908B2 (en) 2012-04-19

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