JP2011083296A5 - - Google Patents
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- Publication number
- JP2011083296A5 JP2011083296A5 JP2011021710A JP2011021710A JP2011083296A5 JP 2011083296 A5 JP2011083296 A5 JP 2011083296A5 JP 2011021710 A JP2011021710 A JP 2011021710A JP 2011021710 A JP2011021710 A JP 2011021710A JP 2011083296 A5 JP2011083296 A5 JP 2011083296A5
- Authority
- JP
- Japan
- Prior art keywords
- restriction enzyme
- vector
- promoter
- site
- restriction
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108091008146 restriction endonucleases Proteins 0.000 claims 40
- 238000000034 method Methods 0.000 claims 35
- 108020004414 DNA Proteins 0.000 claims 15
- 239000013598 vector Substances 0.000 claims 15
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 14
- 210000004027 cell Anatomy 0.000 claims 10
- 239000012634 fragment Substances 0.000 claims 10
- 108090000623 proteins and genes Proteins 0.000 claims 7
- 102000053602 DNA Human genes 0.000 claims 6
- 239000003550 marker Substances 0.000 claims 6
- 108010016529 Bacillus amyloliquefaciens ribonuclease Proteins 0.000 claims 5
- 108020004682 Single-Stranded DNA Proteins 0.000 claims 5
- 230000000295 complement effect Effects 0.000 claims 5
- 150000007523 nucleic acids Chemical group 0.000 claims 5
- 210000001236 prokaryotic cell Anatomy 0.000 claims 5
- SHZGCJCMOBCMKK-UHFFFAOYSA-N D-mannomethylose Natural products CC1OC(O)C(O)C(O)C1O SHZGCJCMOBCMKK-UHFFFAOYSA-N 0.000 claims 4
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 claims 4
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 claims 4
- SHZGCJCMOBCMKK-JFNONXLTSA-N L-rhamnopyranose Chemical compound C[C@@H]1OC(O)[C@H](O)[C@H](O)[C@H]1O SHZGCJCMOBCMKK-JFNONXLTSA-N 0.000 claims 4
- PNNNRSAQSRJVSB-UHFFFAOYSA-N L-rhamnose Natural products CC(O)C(O)C(O)C(O)C=O PNNNRSAQSRJVSB-UHFFFAOYSA-N 0.000 claims 4
- 108700026244 Open Reading Frames Proteins 0.000 claims 4
- 239000002299 complementary DNA Substances 0.000 claims 4
- 238000000338 in vitro Methods 0.000 claims 4
- 239000013612 plasmid Substances 0.000 claims 4
- 239000013604 expression vector Substances 0.000 claims 3
- 230000003248 secreting effect Effects 0.000 claims 3
- 108700007698 Genetic Terminator Regions Proteins 0.000 claims 2
- 108020001507 fusion proteins Proteins 0.000 claims 2
- 102000037865 fusion proteins Human genes 0.000 claims 2
- 230000001939 inductive effect Effects 0.000 claims 2
- 238000013518 transcription Methods 0.000 claims 2
- 230000035897 transcription Effects 0.000 claims 2
- 101710183938 Barstar Proteins 0.000 claims 1
- 108020004511 Recombinant DNA Proteins 0.000 claims 1
- 210000004899 c-terminal region Anatomy 0.000 claims 1
- 230000015556 catabolic process Effects 0.000 claims 1
- 230000003197 catalytic effect Effects 0.000 claims 1
- 230000006698 induction Effects 0.000 claims 1
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US10/678,961 | 2003-10-03 | ||
| US10/678,961 US8293503B2 (en) | 2003-10-03 | 2003-10-03 | Vectors for directional cloning |
| US10/702,228 US20050074785A1 (en) | 2003-10-03 | 2003-11-05 | Vectors for directional cloning |
| US10/702,228 | 2003-11-05 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2006534051A Division JP5089984B2 (ja) | 2003-10-03 | 2004-09-29 | 方向性クローニング用ベクター |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2013168742A Division JP5899168B2 (ja) | 2003-10-03 | 2013-08-14 | 方向性クローニング用ベクター |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2011083296A JP2011083296A (ja) | 2011-04-28 |
| JP2011083296A5 true JP2011083296A5 (enExample) | 2012-10-18 |
| JP5456706B2 JP5456706B2 (ja) | 2014-04-02 |
Family
ID=34394063
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2011021710A Expired - Lifetime JP5456706B2 (ja) | 2003-10-03 | 2011-02-03 | 方向性クローニング用ベクター |
| JP2013168742A Expired - Lifetime JP5899168B2 (ja) | 2003-10-03 | 2013-08-14 | 方向性クローニング用ベクター |
| JP2016009792A Expired - Fee Related JP6341941B2 (ja) | 2003-10-03 | 2016-01-21 | 方向性クローニング用ベクター |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2013168742A Expired - Lifetime JP5899168B2 (ja) | 2003-10-03 | 2013-08-14 | 方向性クローニング用ベクター |
| JP2016009792A Expired - Fee Related JP6341941B2 (ja) | 2003-10-03 | 2016-01-21 | 方向性クローニング用ベクター |
Country Status (6)
| Country | Link |
|---|---|
| US (7) | US8293503B2 (enExample) |
| EP (1) | EP2116601B1 (enExample) |
| JP (3) | JP5456706B2 (enExample) |
| AT (2) | ATE539156T1 (enExample) |
| DE (2) | DE602004024107D1 (enExample) |
| DK (2) | DK1685247T3 (enExample) |
Families Citing this family (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080050808A1 (en) * | 2002-10-09 | 2008-02-28 | Reed Thomas D | DNA modular cloning vector plasmids and methods for their use |
| US7785871B2 (en) * | 2002-10-09 | 2010-08-31 | Intrexon Corporation | DNA cloning vector plasmids and methods for their use |
| WO2005087932A2 (en) * | 2003-10-03 | 2005-09-22 | Promega Corporation | Vectors for directional cloning |
| US8293503B2 (en) | 2003-10-03 | 2012-10-23 | Promega Corporation | Vectors for directional cloning |
| CA2558749A1 (en) * | 2004-02-27 | 2005-09-29 | President And Fellows Of Harvard College | Polynucleotide synthesis |
| US20050227316A1 (en) * | 2004-04-07 | 2005-10-13 | Kosan Biosciences, Inc. | Synthetic genes |
| CN1981047B (zh) | 2004-05-18 | 2013-08-21 | 英特拉克森公司 | Dna克隆载体质粒的动态载体装配方法 |
| US20070004041A1 (en) * | 2005-06-30 | 2007-01-04 | Codon Devices, Inc. | Heirarchical assembly methods for genome engineering |
| US8153598B2 (en) * | 2005-10-19 | 2012-04-10 | Intrexon Corporation | PKD ligands and polynucleotides encoding PKD ligands |
| EP1842915A1 (en) * | 2006-04-04 | 2007-10-10 | Libragen | Method of in vitro polynucleotide sequences shuffling by recursive circular DNA molecules fragmentation and ligation |
| EP2049668A2 (en) * | 2006-08-03 | 2009-04-22 | Wisconsin Alumni Research Foundation | Vector systems |
| WO2008051619A2 (en) * | 2006-10-24 | 2008-05-02 | Los Alamos National Security, Llc | Vectors and methods for selecting open reading frames |
| CA2667697A1 (en) | 2006-10-30 | 2008-05-08 | Promega Corporation | Mutant hydrolase proteins with enhanced kinetics and functional expression |
| US8323930B2 (en) * | 2007-07-28 | 2012-12-04 | Dna Twopointo, Inc. | Methods, compositions and kits for one-step DNA cloning using DNA topoisomerase |
| US20140322708A1 (en) * | 2011-06-02 | 2014-10-30 | Albert Einstein College Of Medicine Of Yeshiva University | Method for measuring somatic dna mutational profiles |
| EP2802655A4 (en) * | 2012-01-13 | 2015-11-18 | Momenta Pharmaceuticals Inc | NUCLEOTIDE CLONING METHODS |
| US10829772B2 (en) | 2012-06-22 | 2020-11-10 | Monsanto Technology Llc | Unique modular vector design |
| US11072811B2 (en) | 2013-03-15 | 2021-07-27 | Promega Corporation | Substrates for covalent tethering of proteins to functional groups or solid surfaces |
| US10676723B2 (en) | 2015-05-11 | 2020-06-09 | David Gordon Bermudes | Chimeric protein toxins for expression by therapeutic bacteria |
| KR20180002795A (ko) * | 2015-05-13 | 2018-01-08 | 칼리뮨, 인코포레이티드 | 렌티바이러스 벡터의 바이오-생산 |
| CN106435744B (zh) * | 2015-08-13 | 2022-02-01 | 生捷科技控股公司 | 使用y-接头和消失限制位点的文库构建 |
| RU2610179C1 (ru) * | 2015-11-06 | 2017-02-08 | Федеральное государственное бюджетное учреждение науки институт биоорганической химии им. академиков М.М. Шемякина и Ю.А. Овчинникова Российской академии наук (ИБХ РАН) | Способ получения рекомбинантного противоопухолевого токсина на основе белков барназа-барстар и адресного полипептида дарпина с эффектом моментальной отмены цитотоксического действия |
| WO2017139412A1 (en) * | 2016-02-09 | 2017-08-17 | Brookhaven Science Associates, Llc | Improved cloning and expression vectors and systems |
| CN106222186B (zh) * | 2016-08-22 | 2019-03-19 | 河北省农林科学院粮油作物研究所 | 入门载体pAmpGate和pGentGate的构建及使用方法 |
| US10435695B2 (en) | 2016-09-08 | 2019-10-08 | The Government of the United States of America, as represented by the Secretary of Homeland Security | Fusion protein comprising Gaussia luciferase, translation interrupter sequence, and interferon amino acid sequences |
| US10385319B2 (en) | 2016-09-08 | 2019-08-20 | The Governement of the United States of America, as represented by the Secretary of Homeland Security | Modified foot-and-mouth disease virus 3C proteases, compositions and methods thereof |
| US11129906B1 (en) | 2016-12-07 | 2021-09-28 | David Gordon Bermudes | Chimeric protein toxins for expression by therapeutic bacteria |
| AU2020205621A1 (en) * | 2019-01-09 | 2021-08-19 | Asimov, Inc. | Compositions of polynucleic acid vectors and uses thereof |
| CN111235638A (zh) * | 2020-02-20 | 2020-06-05 | 北京良芯生物科技发展有限公司 | 一种构建测序文库的方法及试剂盒 |
| CN115698271B (zh) * | 2020-06-12 | 2025-07-18 | 南京金斯瑞生物科技有限公司 | 含barstar基因的工程菌及其在barnase基因克隆中的应用 |
| EP3981879A1 (en) * | 2020-10-09 | 2022-04-13 | Stada Arzneimittel Ag | Method of producing a recombinant protein in a host cell which has a disabled rhamnose metabolism as well as expression vectors, host cells and recombinant proteins thereof |
| GB2638211A (en) * | 2024-02-15 | 2025-08-20 | Nunabio Ltd | A nucleic acid assembly method using Type IIB enzymes |
Family Cites Families (61)
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|---|---|---|---|---|
| US815951A (en) | 1903-10-15 | 1906-03-27 | American Textile Specialty Machinery Company | Weft-replenishing loom. |
| US4403036A (en) | 1980-12-02 | 1983-09-06 | University Of Iowa Research Foundation | Genetic reagents for generating plasmids containing multiple copies of DNA segments |
| US4724202A (en) | 1983-12-12 | 1988-02-09 | Molecular Diagnostics, Inc. | Use of non-hybridizable nucleic acids for the detection of nucleic acid hybridization |
| US4784949A (en) | 1984-04-19 | 1988-11-15 | Cetus Corporation | Universal dominant selectable marker cassette |
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| US5506126A (en) | 1988-02-25 | 1996-04-09 | The General Hospital Corporation | Rapid immunoselection cloning method |
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| FR2824844B1 (fr) | 2001-05-18 | 2003-09-19 | Genoway | Procede de clonage par double selection et vecteurs pour ce procede |
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| US8293503B2 (en) | 2003-10-03 | 2012-10-23 | Promega Corporation | Vectors for directional cloning |
-
2003
- 2003-10-03 US US10/678,961 patent/US8293503B2/en not_active Expired - Fee Related
- 2003-11-05 US US10/702,228 patent/US20050074785A1/en not_active Abandoned
-
2004
- 2004-09-29 DE DE200460024107 patent/DE602004024107D1/de not_active Expired - Lifetime
- 2004-09-29 DK DK04821603T patent/DK1685247T3/da active
- 2004-09-29 EP EP20090011477 patent/EP2116601B1/en not_active Expired - Lifetime
- 2004-09-29 DE DE200460023140 patent/DE602004023140D1/de not_active Expired - Lifetime
- 2004-09-29 AT AT09011477T patent/ATE539156T1/de active
- 2004-09-29 AT AT04821603T patent/ATE448303T1/de not_active IP Right Cessation
- 2004-09-29 DK DK07013756T patent/DK1847611T3/da active
- 2004-11-12 US US10/987,411 patent/US20050130205A1/en not_active Abandoned
-
2009
- 2009-12-28 US US12/648,119 patent/US8367403B2/en not_active Expired - Fee Related
-
2011
- 2011-02-03 JP JP2011021710A patent/JP5456706B2/ja not_active Expired - Lifetime
- 2011-10-17 US US13/274,637 patent/US9469857B2/en not_active Expired - Fee Related
- 2011-10-17 US US13/274,643 patent/US9018014B2/en not_active Expired - Lifetime
-
2013
- 2013-01-14 US US13/740,337 patent/US9371531B2/en not_active Expired - Fee Related
- 2013-08-14 JP JP2013168742A patent/JP5899168B2/ja not_active Expired - Lifetime
-
2016
- 2016-01-21 JP JP2016009792A patent/JP6341941B2/ja not_active Expired - Fee Related
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