JP2023156365A5 - - Google Patents
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- Publication number
- JP2023156365A5 JP2023156365A5 JP2023122299A JP2023122299A JP2023156365A5 JP 2023156365 A5 JP2023156365 A5 JP 2023156365A5 JP 2023122299 A JP2023122299 A JP 2023122299A JP 2023122299 A JP2023122299 A JP 2023122299A JP 2023156365 A5 JP2023156365 A5 JP 2023156365A5
- Authority
- JP
- Japan
- Prior art keywords
- cell
- protein
- linker
- fusion protein
- nuclear localization
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 108020004707 nucleic acids Proteins 0.000 claims 20
- 102000039446 nucleic acids Human genes 0.000 claims 20
- 150000007523 nucleic acids Chemical class 0.000 claims 20
- 108020001507 fusion proteins Proteins 0.000 claims 16
- 102000037865 fusion proteins Human genes 0.000 claims 16
- 108090000623 proteins and genes Proteins 0.000 claims 15
- 102000004169 proteins and genes Human genes 0.000 claims 15
- 239000003550 marker Substances 0.000 claims 14
- 108020005004 Guide RNA Proteins 0.000 claims 12
- 210000004027 cell Anatomy 0.000 claims 11
- 210000003527 eukaryotic cell Anatomy 0.000 claims 11
- 108010077850 Nuclear Localization Signals Proteins 0.000 claims 6
- 125000003275 alpha amino acid group Chemical group 0.000 claims 5
- 108091033409 CRISPR Proteins 0.000 claims 4
- 230000004807 localization Effects 0.000 claims 4
- 241000206602 Eukaryota Species 0.000 claims 3
- 241000251539 Vertebrata <Metazoa> Species 0.000 claims 3
- 210000005260 human cell Anatomy 0.000 claims 3
- 238000000338 in vitro Methods 0.000 claims 3
- 210000004962 mammalian cell Anatomy 0.000 claims 3
- 108020004414 DNA Proteins 0.000 claims 2
- 108010008532 Deoxyribonuclease I Proteins 0.000 claims 2
- 102000007260 Deoxyribonuclease I Human genes 0.000 claims 2
- 101710163270 Nuclease Proteins 0.000 claims 2
- 230000006819 RNA synthesis Effects 0.000 claims 2
- 238000000034 method Methods 0.000 claims 2
- 230000030648 nucleus localization Effects 0.000 claims 2
- 230000001737 promoting effect Effects 0.000 claims 2
- 239000013598 vector Substances 0.000 claims 2
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims 1
- 108010077544 Chromatin Proteins 0.000 claims 1
- 208000009869 Neu-Laxova syndrome Diseases 0.000 claims 1
- 108091008103 RNA aptamers Proteins 0.000 claims 1
- 241000193996 Streptococcus pyogenes Species 0.000 claims 1
- 108020000999 Viral RNA Proteins 0.000 claims 1
- 230000001580 bacterial effect Effects 0.000 claims 1
- 210000004899 c-terminal region Anatomy 0.000 claims 1
- 210000003483 chromatin Anatomy 0.000 claims 1
- 230000002759 chromosomal effect Effects 0.000 claims 1
- 230000000694 effects Effects 0.000 claims 1
- 230000004049 epigenetic modification Effects 0.000 claims 1
- 238000001727 in vivo Methods 0.000 claims 1
- 230000035699 permeability Effects 0.000 claims 1
- 239000013600 plasmid vector Substances 0.000 claims 1
- 230000022532 regulation of transcription, DNA-dependent Effects 0.000 claims 1
- 230000001105 regulatory effect Effects 0.000 claims 1
- 238000013518 transcription Methods 0.000 claims 1
- 230000035897 transcription Effects 0.000 claims 1
- 239000013603 viral vector Substances 0.000 claims 1
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201962806708P | 2019-02-15 | 2019-02-15 | |
| US62/806,708 | 2019-02-15 | ||
| JP2021547390A JP2022520104A (ja) | 2019-02-15 | 2020-02-13 | Ｃｒｉｓｐｒ／ｃａｓ融合タンパク質およびシステム |
| PCT/US2020/018145 WO2020168102A1 (en) | 2019-02-15 | 2020-02-13 | Crispr/cas fusion proteins and systems |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2021547390A Division JP2022520104A (ja) | 2019-02-15 | 2020-02-13 | Ｃｒｉｓｐｒ／ｃａｓ融合タンパク質およびシステム |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2023156365A JP2023156365A (ja) | 2023-10-24 |
| JP2023156365A5 true JP2023156365A5 (enExample) | 2024-04-26 |
Family
ID=70166116
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2021547390A Pending JP2022520104A (ja) | 2019-02-15 | 2020-02-13 | Ｃｒｉｓｐｒ／ｃａｓ融合タンパク質およびシステム |
| JP2023122299A Pending JP2023156365A (ja) | 2019-02-15 | 2023-07-27 | Ｃｒｉｓｐｒ／ｃａｓ融合タンパク質およびシステム |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2021547390A Pending JP2022520104A (ja) | 2019-02-15 | 2020-02-13 | Ｃｒｉｓｐｒ／ｃａｓ融合タンパク質およびシステム |
Country Status (11)
| Country | Link |
|---|---|
| US (3) | US10947517B2 (enExample) |
| EP (1) | EP3924475A1 (enExample) |
| JP (2) | JP2022520104A (enExample) |
| KR (1) | KR20210139271A (enExample) |
| CN (1) | CN113728099A (enExample) |
| AU (2) | AU2020221274B2 (enExample) |
| BR (1) | BR112021016019A2 (enExample) |
| CA (1) | CA3129835A1 (enExample) |
| IL (1) | IL285300A (enExample) |
| SG (1) | SG11202108812SA (enExample) |
| WO (1) | WO2020168102A1 (enExample) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20180250424A1 (en) | 2014-10-10 | 2018-09-06 | Editas Medicine, Inc. | Compositions and methods for promoting homology directed repair |
| CA3129835A1 (en) | 2019-02-15 | 2020-08-20 | Sigma-Aldrich Co. Llc | Crispr/cas fusion proteins and systems |
| MX2024002927A (es) * | 2021-09-08 | 2024-05-29 | Flagship Pioneering Innovations Vi Llc | Metodos y composiciones para modular un genoma. |
| CN116063546A (zh) * | 2021-10-29 | 2023-05-05 | 深圳先进技术研究院 | 一种基于共振能量转移的全遗传编码nad+蛋白探针及其制备方法和应用 |
| US20230265405A1 (en) * | 2022-02-22 | 2023-08-24 | Massachusetts Institute Of Technology | Engineered nucleases and methods of use thereof |
| WO2025221793A1 (en) * | 2024-04-16 | 2025-10-23 | Monsanto Technology Llc | Modified cas proteins for nuclear localization and editing activity in plants |
Family Cites Families (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2002360424A1 (en) | 2001-11-26 | 2003-06-10 | Advanced Cell Technology, Inc. | Methods for making and using reprogrammed human somatic cell nuclei and autologous and isogenic human stem cells |
| RS59199B1 (sr) | 2012-05-25 | 2019-10-31 | Univ California | Metode i jedinjenja za rnk-upravljanu ciljanu dnk modifikaciju i za rnk- upravljanu modulaciju transkripta |
| KR102479178B1 (ko) | 2012-12-06 | 2022-12-19 | 시그마-알드리치 컴퍼니., 엘엘씨 | Crispr-기초된 유전체 변형과 조절 |
| WO2014186686A2 (en) * | 2013-05-17 | 2014-11-20 | Two Blades Foundation | Targeted mutagenesis and genome engineering in plants using rna-guided cas nucleases |
| DK3007729T3 (da) * | 2013-06-11 | 2019-09-30 | Portage Pharmaceuticals Ltd | Struktur, fremstilling og anvendelser af humanderiverede cellegennemtrængelige peptider, der er konjugeret med specifikke biologisk aktive cargo-peptider |
| WO2015089486A2 (en) | 2013-12-12 | 2015-06-18 | The Broad Institute Inc. | Systems, methods and compositions for sequence manipulation with optimized functional crispr-cas systems |
| AU2015219339B2 (en) * | 2014-02-19 | 2020-03-05 | Bioasis Technologies Inc. | P97-IDS fusion proteins |
| WO2015148863A2 (en) | 2014-03-26 | 2015-10-01 | Editas Medicine, Inc. | Crispr/cas-related methods and compositions for treating sickle cell disease |
| IL287561B2 (en) | 2014-10-01 | 2024-03-01 | Massachusetts Gen Hospital | Methods for increasing efficiency of nuclease-induced homology-directed repair |
| WO2016210271A1 (en) | 2015-06-24 | 2016-12-29 | Sigma-Aldrich Co. Llc | Cell cycle dependent genome regulation and modification |
| WO2017131150A1 (ja) * | 2016-01-29 | 2017-08-03 | 国立大学法人東京大学 | Ｃａｓ９－ｇＲＮＡ複合体の製造方法、Ｃａｓ９－ｇＲＮＡ複合体の細胞核内への導入方法、及び細胞内における標的遺伝子の改変方法 |
| WO2017173004A1 (en) * | 2016-03-30 | 2017-10-05 | Mikuni Takayasu | A method for in vivo precise genome editing |
| US11236313B2 (en) | 2016-04-13 | 2022-02-01 | Editas Medicine, Inc. | Cas9 fusion molecules, gene editing systems, and methods of use thereof |
| CN107579793A (zh) * | 2016-07-04 | 2018-01-12 | 中兴通讯股份有限公司 | 一种通信网络设备间时间同步的优化方法、装置及设备 |
| GB2568182A (en) * | 2016-08-03 | 2019-05-08 | Harvard College | Adenosine nucleobase editors and uses thereof |
| WO2018031683A1 (en) | 2016-08-09 | 2018-02-15 | President And Fellows Of Harvard College | Programmable cas9-recombinase fusion proteins and uses thereof |
| GB2573062A (en) * | 2016-10-14 | 2019-10-23 | Harvard College | AAV delivery of nucleobase editors |
| KR20190104342A (ko) | 2016-12-14 | 2019-09-09 | 바게닝겐 유니버시테이트 | 열안정성 cas9 뉴클레아제 |
| US11898179B2 (en) | 2017-03-09 | 2024-02-13 | President And Fellows Of Harvard College | Suppression of pain by gene editing |
| GB201710973D0 (en) | 2017-07-07 | 2017-08-23 | Avacta Life Sciences Ltd | Scaffold proteins |
| TWI820034B (zh) | 2017-07-31 | 2023-11-01 | 香港商映像生物有限公司 | 眼部疾病之細胞模式及用於眼部疾病的療法 |
| US11071791B2 (en) * | 2018-01-26 | 2021-07-27 | Wisconsin Alumni Research Foundation | Vector for gene silencing and replacement and methods of use thereof |
| US10767193B2 (en) | 2018-02-15 | 2020-09-08 | Sigma-Aldrich Co. Llc | Engineered CAS9 systems for eukaryotic genome modification |
| CN112041444A (zh) * | 2018-03-14 | 2020-12-04 | 阿伯生物技术公司 | 新型crispr dna靶向酶及系统 |
| CA3129835A1 (en) | 2019-02-15 | 2020-08-20 | Sigma-Aldrich Co. Llc | Crispr/cas fusion proteins and systems |
-
2020
- 2020-02-13 CA CA3129835A patent/CA3129835A1/en active Pending
- 2020-02-13 WO PCT/US2020/018145 patent/WO2020168102A1/en not_active Ceased
- 2020-02-13 KR KR1020217029732A patent/KR20210139271A/ko not_active Ceased
- 2020-02-13 AU AU2020221274A patent/AU2020221274B2/en not_active Ceased
- 2020-02-13 SG SG11202108812SA patent/SG11202108812SA/en unknown
- 2020-02-13 US US16/790,399 patent/US10947517B2/en active Active
- 2020-02-13 EP EP20716979.8A patent/EP3924475A1/en active Pending
- 2020-02-13 BR BR112021016019-1A patent/BR112021016019A2/pt unknown
- 2020-02-13 CN CN202080029346.8A patent/CN113728099A/zh active Pending
- 2020-02-13 JP JP2021547390A patent/JP2022520104A/ja active Pending
-
2021
- 2021-02-10 US US17/172,734 patent/US11965184B2/en active Active
- 2021-08-02 IL IL285300A patent/IL285300A/en unknown
-
2023
- 2023-04-27 US US18/308,336 patent/US20230287377A1/en not_active Abandoned
- 2023-07-27 JP JP2023122299A patent/JP2023156365A/ja active Pending
-
2024
- 2024-04-09 AU AU2024202275A patent/AU2024202275A1/en not_active Abandoned
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