JPWO2020181102A5 - - Google Patents
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- Publication number
- JPWO2020181102A5 JPWO2020181102A5 JP2021548244A JP2021548244A JPWO2020181102A5 JP WO2020181102 A5 JPWO2020181102 A5 JP WO2020181102A5 JP 2021548244 A JP2021548244 A JP 2021548244A JP 2021548244 A JP2021548244 A JP 2021548244A JP WO2020181102 A5 JPWO2020181102 A5 JP WO2020181102A5
- Authority
- JP
- Japan
- Prior art keywords
- crispr
- activity
- cas effector
- polypeptide
- nucleic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 229920001184 polypeptide Polymers 0.000 claims 50
- 108090000765 processed proteins & peptides Proteins 0.000 claims 50
- 102000004196 processed proteins & peptides Human genes 0.000 claims 50
- 239000012636 effector Substances 0.000 claims 47
- 230000000694 effects Effects 0.000 claims 36
- 108020004707 nucleic acids Proteins 0.000 claims 21
- 102000039446 nucleic acids Human genes 0.000 claims 21
- 150000007523 nucleic acids Chemical class 0.000 claims 21
- 108020005004 Guide RNA Proteins 0.000 claims 14
- 230000004927 fusion Effects 0.000 claims 12
- 210000004027 cell Anatomy 0.000 claims 10
- 125000003275 alpha amino acid group Chemical group 0.000 claims 9
- 238000000034 method Methods 0.000 claims 6
- 239000002773 nucleotide Substances 0.000 claims 6
- 125000003729 nucleotide group Chemical group 0.000 claims 6
- 239000013598 vector Substances 0.000 claims 5
- 108020004414 DNA Proteins 0.000 claims 4
- 108010077850 Nuclear Localization Signals Proteins 0.000 claims 4
- 230000002255 enzymatic effect Effects 0.000 claims 4
- 238000000338 in vitro Methods 0.000 claims 4
- 230000000051 modifying effect Effects 0.000 claims 4
- 108060004795 Methyltransferase Proteins 0.000 claims 3
- 102000004389 Ribonucleoproteins Human genes 0.000 claims 3
- 108010081734 Ribonucleoproteins Proteins 0.000 claims 3
- 102000004169 proteins and genes Human genes 0.000 claims 3
- 108090000623 proteins and genes Proteins 0.000 claims 3
- 102000007260 Deoxyribonuclease I Human genes 0.000 claims 2
- 108010008532 Deoxyribonuclease I Proteins 0.000 claims 2
- 101000615488 Homo sapiens Methyl-CpG-binding domain protein 2 Proteins 0.000 claims 2
- 102100021299 Methyl-CpG-binding domain protein 2 Human genes 0.000 claims 2
- 102000016397 Methyltransferase Human genes 0.000 claims 2
- 101710163270 Nuclease Proteins 0.000 claims 2
- 239000013604 expression vector Substances 0.000 claims 2
- 230000007498 myristoylation Effects 0.000 claims 2
- 238000003259 recombinant expression Methods 0.000 claims 2
- 230000001177 retroviral effect Effects 0.000 claims 2
- 238000013518 transcription Methods 0.000 claims 2
- 230000035897 transcription Effects 0.000 claims 2
- 230000005778 DNA damage Effects 0.000 claims 1
- 231100000277 DNA damage Toxicity 0.000 claims 1
- 230000033616 DNA repair Effects 0.000 claims 1
- 108010046331 Deoxyribodipyrimidine photo-lyase Proteins 0.000 claims 1
- 241000702421 Dependoparvovirus Species 0.000 claims 1
- 108010033040 Histones Proteins 0.000 claims 1
- 102100034343 Integrase Human genes 0.000 claims 1
- 108010061833 Integrases Proteins 0.000 claims 1
- 102000003960 Ligases Human genes 0.000 claims 1
- 108090000364 Ligases Proteins 0.000 claims 1
- 102100025169 Max-binding protein MNT Human genes 0.000 claims 1
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 1
- 108010077991 O-GlcNAc transferase Proteins 0.000 claims 1
- 102000005520 O-GlcNAc transferase Human genes 0.000 claims 1
- 230000010718 Oxidation Activity Effects 0.000 claims 1
- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 claims 1
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 claims 1
- 108091000080 Phosphotransferase Proteins 0.000 claims 1
- 229940124158 Protease/peptidase inhibitor Drugs 0.000 claims 1
- CZPWVGJYEJSRLH-UHFFFAOYSA-N Pyrimidine Chemical compound C1=CN=CN=C1 CZPWVGJYEJSRLH-UHFFFAOYSA-N 0.000 claims 1
- 102000018120 Recombinases Human genes 0.000 claims 1
- 108010091086 Recombinases Proteins 0.000 claims 1
- 102000008579 Transposases Human genes 0.000 claims 1
- 108010020764 Transposases Proteins 0.000 claims 1
- 102000006275 Ubiquitin-Protein Ligases Human genes 0.000 claims 1
- 108010083111 Ubiquitin-Protein Ligases Proteins 0.000 claims 1
- 102000005421 acetyltransferase Human genes 0.000 claims 1
- 108020002494 acetyltransferase Proteins 0.000 claims 1
- 230000006154 adenylylation Effects 0.000 claims 1
- 230000029936 alkylation Effects 0.000 claims 1
- 238000005804 alkylation reaction Methods 0.000 claims 1
- 108010031100 chloroplast transit peptides Proteins 0.000 claims 1
- 230000009615 deamination Effects 0.000 claims 1
- 238000006481 deamination reaction Methods 0.000 claims 1
- 230000007423 decrease Effects 0.000 claims 1
- 230000022811 deglycosylation Effects 0.000 claims 1
- 230000027832 depurination Effects 0.000 claims 1
- 230000029180 desumoylation Effects 0.000 claims 1
- 238000006471 dimerization reaction Methods 0.000 claims 1
- 230000006806 disease prevention Effects 0.000 claims 1
- 210000003527 eukaryotic cell Anatomy 0.000 claims 1
- 230000013595 glycosylation Effects 0.000 claims 1
- 238000006206 glycosylation reaction Methods 0.000 claims 1
- 230000002401 inhibitory effect Effects 0.000 claims 1
- 239000002502 liposome Substances 0.000 claims 1
- 230000004048 modification Effects 0.000 claims 1
- 238000012986 modification Methods 0.000 claims 1
- 239000002245 particle Substances 0.000 claims 1
- 239000000137 peptide hydrolase inhibitor Substances 0.000 claims 1
- 102000020233 phosphotransferase Human genes 0.000 claims 1
- 230000029279 positive regulation of transcription, DNA-dependent Effects 0.000 claims 1
- 230000010741 sumoylation Effects 0.000 claims 1
- 230000008685 targeting Effects 0.000 claims 1
- 108091006107 transcriptional repressors Proteins 0.000 claims 1
- 230000026683 transduction Effects 0.000 claims 1
- 238000010361 transduction Methods 0.000 claims 1
- 239000013603 viral vector Substances 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201962815179P | 2019-03-07 | 2019-03-07 | |
| US62/815,179 | 2019-03-07 | ||
| PCT/US2020/021214 WO2020181102A1 (en) | 2019-03-07 | 2020-03-05 | Crispr-cas effector polypeptides and methods of use thereof |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2022522650A JP2022522650A (ja) | 2022-04-20 |
| JPWO2020181102A5 true JPWO2020181102A5 (enExample) | 2023-03-13 |
| JP7649748B2 JP7649748B2 (ja) | 2025-03-21 |
Family
ID=72338076
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2021548244A Active JP7649748B2 (ja) | 2019-03-07 | 2020-03-05 | Ｃｒｉｓｐｒ－ｃａｓエフェクターポリペプチド及びその使用方法 |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US12312614B2 (enExample) |
| EP (1) | EP3935155A4 (enExample) |
| JP (1) | JP7649748B2 (enExample) |
| CN (1) | CN114040971A (enExample) |
| WO (1) | WO2020181102A1 (enExample) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3204496A1 (en) | 2014-10-10 | 2017-08-16 | Editas Medicine, Inc. | Compositions and methods for promoting homology directed repair |
| US10660419B2 (en) * | 2016-12-15 | 2020-05-26 | Elc Management Llc | Packaged skin treatment composition and method |
| AU2019291918B2 (en) | 2018-06-29 | 2025-06-12 | Editas Medicine, Inc. | Synthetic guide molecules, compositions and methods relating thereto |
| US10894812B1 (en) | 2020-09-30 | 2021-01-19 | Alpine Roads, Inc. | Recombinant milk proteins |
| US10947552B1 (en) | 2020-09-30 | 2021-03-16 | Alpine Roads, Inc. | Recombinant fusion proteins for producing milk proteins in plants |
| CA3191387A1 (en) | 2020-09-30 | 2022-04-07 | Nobell Foods, Inc. | Recombinant milk proteins and food compositions comprising the same |
| EP4347818A2 (en) | 2021-06-01 | 2024-04-10 | Arbor Biotechnologies, Inc. | Gene editing systems comprising a crispr nuclease and uses thereof |
| WO2024006824A2 (en) * | 2022-06-28 | 2024-01-04 | Mammoth Biosciences, Inc. | Effector proteins, compositions, systems and methods of use thereof |
| JP2025540708A (ja) | 2022-11-23 | 2025-12-16 | アンバー バイオ インコーポレイテッド | 遺伝子を修飾するエンドヌクレアーゼ |
| IL322416A (en) | 2023-02-15 | 2025-09-01 | Arbor Biotechnologies Inc | Gene editing method to inhibit abnormal splicing in stathmin 2 (stmn2) transcription |
| WO2025171210A1 (en) | 2024-02-09 | 2025-08-14 | Arbor Biotechnologies, Inc. | Compositions and methods for gene editing via homology-mediated end joining |
| WO2026015832A2 (en) | 2024-07-12 | 2026-01-15 | Arbor Biotechnologies, Inc. | Reverse transcriptases and gene editing systems comprising such |
| WO2026015829A2 (en) | 2024-07-12 | 2026-01-15 | Arbor Biotechnologies, Inc. | Small reverse transcriptases and gene editing systems comprising such |
| CN119372181A (zh) * | 2024-09-14 | 2025-01-28 | 尧唐(南京)生物科技有限公司 | 一种核酸酶蛋白及其应用 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6605709B1 (en) * | 1999-04-09 | 2003-08-12 | Genome Therapeutics Corporation | Nucleic acid and amino acid sequences relating to Proteus mirabilis for diagnostics and therapeutics |
| US20040029129A1 (en) * | 2001-10-25 | 2004-02-12 | Liangsu Wang | Identification of essential genes in microorganisms |
| WO2005024044A2 (en) * | 2003-09-05 | 2005-03-17 | Gtc Biotherapeutics, Inc. | Method for the production of fusion proteins in transgenic mammal milk |
| AU2011325956B2 (en) * | 2010-11-12 | 2016-07-14 | The General Hospital Corporation | Polycomb-associated non-coding RNAs |
| EP3080260B1 (en) | 2013-12-12 | 2019-03-06 | The Broad Institute, Inc. | Crispr-cas systems and methods for altering expression of gene products, structural information and inducible modular cas enzymes |
| US9790490B2 (en) * | 2015-06-18 | 2017-10-17 | The Broad Institute Inc. | CRISPR enzymes and systems |
| CN115216459B (zh) * | 2015-12-29 | 2024-06-28 | 孟山都技术公司 | 新型crispr相关转座酶及其用途 |
| WO2017189308A1 (en) * | 2016-04-19 | 2017-11-02 | The Broad Institute Inc. | Novel crispr enzymes and systems |
| US11692184B2 (en) | 2017-05-16 | 2023-07-04 | The Regents Of The University Of California | Thermostable RNA-guided endonucleases and methods of use thereof |
| GB201712733D0 (en) * | 2017-08-08 | 2017-09-20 | Snipr Tech Ltd | Methods & cells |
-
2020
- 2020-03-05 EP EP20767083.7A patent/EP3935155A4/en active Pending
- 2020-03-05 JP JP2021548244A patent/JP7649748B2/ja active Active
- 2020-03-05 WO PCT/US2020/021214 patent/WO2020181102A1/en not_active Ceased
- 2020-03-05 CN CN202080030261.1A patent/CN114040971A/zh active Pending
-
2021
- 2021-08-16 US US17/403,236 patent/US12312614B2/en active Active
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