JP2007508012A5 - - Google Patents

Download PDF

Info

Publication number
JP2007508012A5
JP2007508012A5 JP2006534051A JP2006534051A JP2007508012A5 JP 2007508012 A5 JP2007508012 A5 JP 2007508012A5 JP 2006534051 A JP2006534051 A JP 2006534051A JP 2006534051 A JP2006534051 A JP 2006534051A JP 2007508012 A5 JP2007508012 A5 JP 2007508012A5
Authority
JP
Japan
Prior art keywords
restriction enzyme
vector
open reading
reading frame
site
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2006534051A
Other languages
English (en)
Japanese (ja)
Other versions
JP2007508012A (ja
JP5089984B2 (ja
Filing date
Publication date
Priority claimed from US10/678,961 external-priority patent/US8293503B2/en
Application filed filed Critical
Priority claimed from PCT/US2004/031912 external-priority patent/WO2005087932A2/en
Publication of JP2007508012A publication Critical patent/JP2007508012A/ja
Publication of JP2007508012A5 publication Critical patent/JP2007508012A5/ja
Application granted granted Critical
Publication of JP5089984B2 publication Critical patent/JP5089984B2/ja
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

JP2006534051A 2003-10-03 2004-09-29 方向性クローニング用ベクター Expired - Fee Related JP5089984B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US10/678,961 US8293503B2 (en) 2003-10-03 2003-10-03 Vectors for directional cloning
US10/678,961 2003-10-03
US10/702,228 US20050074785A1 (en) 2003-10-03 2003-11-05 Vectors for directional cloning
US10/702,228 2003-11-05
PCT/US2004/031912 WO2005087932A2 (en) 2003-10-03 2004-09-29 Vectors for directional cloning

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2011021710A Division JP5456706B2 (ja) 2003-10-03 2011-02-03 方向性クローニング用ベクター

Publications (3)

Publication Number Publication Date
JP2007508012A JP2007508012A (ja) 2007-04-05
JP2007508012A5 true JP2007508012A5 (enExample) 2007-11-08
JP5089984B2 JP5089984B2 (ja) 2012-12-05

Family

ID=34971637

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2006534051A Expired - Fee Related JP5089984B2 (ja) 2003-10-03 2004-09-29 方向性クローニング用ベクター

Country Status (6)

Country Link
US (1) US20070212762A1 (enExample)
EP (2) EP1685247B1 (enExample)
JP (1) JP5089984B2 (enExample)
AT (1) ATE442445T1 (enExample)
CA (1) CA2541177A1 (enExample)
WO (1) WO2005087932A2 (enExample)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8293503B2 (en) 2003-10-03 2012-10-23 Promega Corporation Vectors for directional cloning
US8202727B2 (en) * 2005-05-17 2012-06-19 Ozgene Pty Ltd. Sequential cloning system
AU2008212907A1 (en) * 2007-02-05 2008-08-14 Philipps-Universitat Marburg Method of cloning at least one nucleic acid molecule of interest using type IIS restriction endonucleases, and corresponding cloning vectors, kits and system using type IIS restriction endonucleases
US20110244521A1 (en) 2007-08-21 2011-10-06 Takeharu Nagai Method for preparation of recobinant dna
WO2009079212A2 (en) 2007-12-03 2009-06-25 Carnegie Mellon University Linked peptide fluorogenic biosensors
WO2009106635A1 (en) 2008-02-29 2009-09-03 Xbrane Bioscience Aktiebolag Expression system for proteins
EP2802655A4 (en) * 2012-01-13 2015-11-18 Momenta Pharmaceuticals Inc NUCLEOTIDE CLONING METHODS
US10829772B2 (en) 2012-06-22 2020-11-10 Monsanto Technology Llc Unique modular vector design
US11072811B2 (en) 2013-03-15 2021-07-27 Promega Corporation Substrates for covalent tethering of proteins to functional groups or solid surfaces
WO2014142955A1 (en) * 2013-03-15 2014-09-18 Carnegie Mellon University Linked peptide fluorogenic biosensors
CN104450919A (zh) * 2014-12-12 2015-03-25 南华大学 通过插入部分摇摆寡核苷酸检测基因突变耐受能力的方法
EP4159862A4 (en) * 2020-05-26 2024-06-05 Spiber Inc. Method for preparing combinatorial library of multi-modular biosynthetic enzyme gene

Family Cites Families (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4403036A (en) * 1980-12-02 1983-09-06 University Of Iowa Research Foundation Genetic reagents for generating plasmids containing multiple copies of DNA segments
US4724202A (en) * 1983-12-12 1988-02-09 Molecular Diagnostics, Inc. Use of non-hybridizable nucleic acids for the detection of nucleic acid hybridization
US4784949A (en) * 1984-04-19 1988-11-15 Cetus Corporation Universal dominant selectable marker cassette
US4952496A (en) * 1984-03-30 1990-08-28 Associated Universities, Inc. Cloning and expression of the gene for bacteriophage T7 RNA polymerase
US5693489A (en) * 1984-03-30 1997-12-02 Associated Universities, Inc. Cloning and expression of the gene for bacteriophage T7 RNA polymerase
EP0178863A1 (en) * 1984-10-15 1986-04-23 Schering Corporation Novel expression systems utilizing bacteriophage T7 promoters and gene sequences
US5200333A (en) * 1985-03-01 1993-04-06 New England Biolabs, Inc. Cloning restriction and modification genes
DE3750708T2 (de) * 1986-06-06 1995-06-29 New England Biolabs Inc Verfahren zur Klonierung eines Restriktionsmodifizierungssystems.
US5506126A (en) * 1988-02-25 1996-04-09 The General Hospital Corporation Rapid immunoselection cloning method
WO1991002077A1 (en) * 1989-07-28 1991-02-21 The United States Of America, Represented By The Secretary, United States Department Of Commerce Efficient directional genetic cloning system
US5122457A (en) * 1989-10-19 1992-06-16 Schering Corporation Expression systems utilizing bacteriophage t7 promoters, gene sequences, and t7 rna polymerase
DE69024188T2 (de) * 1990-01-11 1996-08-14 New England Biolabs Inc SfiI-Restriktionsendonuklease und Methylase Gene und Verfahren zu deren Klonierung.
US5179015A (en) * 1990-07-23 1993-01-12 New England Biolabs, Inc. Heterospecific modification as a means to clone restriction genes
JP2739378B2 (ja) * 1990-11-09 1998-04-15 アメリカ合衆国 Ｄｎａに標的を定める方法
US5196330A (en) * 1991-06-03 1993-03-23 New England Biolabs, Inc. Type ii restriction endonuclease, pme i, obtainable from pseudomonas mendocina and a process for producing the same
US5342782A (en) * 1992-02-24 1994-08-30 Washington University Tightly regulated expression system for eucaryotic cells
BE1006085A3 (fr) * 1992-07-31 1994-05-10 Univ Bruxelles Vecteur de clonage.
US6521425B2 (en) * 1997-03-05 2003-02-18 New England Biolabs, Inc. Screening and use of reagents which block or activate intein splicing utilizing natural or homologous exteins
US5731411A (en) * 1993-07-12 1998-03-24 The United States Of America As Represented By The Department Of Health And Human Services Promotion of homologous DNA pairing by RecA-derived peptides
US5391487A (en) * 1993-08-13 1995-02-21 Promega Corporation Restriction endonuclease SgfI from Streptomyces griseoruber
US5498535A (en) * 1994-05-24 1996-03-12 New England Biolabs, Inc. Method for direct cloning of nuclease genes in E. coli
DE19514310A1 (de) * 1995-04-18 1996-10-24 Univ Ludwigs Albert Vektoren zur Transfektion von eukaryotischen Zellen, deren Verwendung und damit transfizierte Zielzellen
WO1997004111A1 (en) * 1995-07-21 1997-02-06 The Government Of The United States Of America, Represented By The Secretary, Department Of Health And Human Services RecA-ASSISTED CLONING OF DNA
US5830690A (en) * 1995-12-29 1998-11-03 Council Of Scientific & Indus. Res. & Dept. Of Biotech. Process for producing polypeptides
US5663067A (en) * 1996-07-11 1997-09-02 New England Biolabs, Inc. Method for cloning and producing the SapI restriction endonuclease in E. coli
DE19653498C1 (de) * 1996-12-20 1998-07-23 Wolf M Prof Dr Bertling Klonierungsvektor-System mit Fusionssequenz
US5851808A (en) * 1997-02-28 1998-12-22 Baylor College Of Medicine Rapid subcloning using site-specific recombination
US6248569B1 (en) * 1997-11-10 2001-06-19 Brookhaven Science Associates Method for introducing unidirectional nested deletions
US5945288A (en) * 1997-11-24 1999-08-31 New England Biolabs, Inc. Method for cloning and producing the PmeI restriction endonuclease
EP1062512A4 (en) * 1998-03-10 2001-12-12 Univ California METHODS AND TOOLS USED TO IDENTIFY COMPOUNDS THAT MODULATE ATHEROSCLEROSIS BY ALTERATION OF THE LDL-PROTEOGLYCANE BINDING
AU5584999A (en) * 1998-08-28 2000-03-21 Invitrogen Corporation System for the rapid manipulation of nucleic acid sequences
US6096523A (en) * 1998-11-04 2000-08-01 University Of Georgia Research Foundation Transformation vector system
US6184000B1 (en) * 1999-07-23 2001-02-06 The United States Of America As Represented By The Secretary Of Agriculture System for the sequential, directional cloning of multiple DNA sequences
US6479280B1 (en) * 1999-09-24 2002-11-12 Vlaams Interuniversitair Institutuut Voor Biotechnologie Vzw Recombinant phages capable of entering host cells via specific interaction with an artificial receptor
WO2001055371A1 (en) * 2000-01-28 2001-08-02 The Scripps Research Institute Methods of identifying synthetic transcriptional and translational regulatory elements, and compositions relating to same
US6908751B2 (en) * 2000-04-26 2005-06-21 Zymogenetics, Inc. Methods for enhancing the expression of a protein of interest by recombinant host cells
US20020086310A1 (en) * 2000-09-01 2002-07-04 Frank Fan Identification of targets of antimicrobial compounds
EP1360490B1 (en) * 2001-01-23 2011-12-21 President and Fellows of Harvard College Nucleic-acid programmable protein arrays
FR2824844B1 (fr) * 2001-05-18 2003-09-19 Genoway Procede de clonage par double selection et vecteurs pour ce procede
US7094579B2 (en) * 2002-02-13 2006-08-22 Xoma Technology Ltd. Eukaryotic signal sequences for prokaryotic expression
US20040166567A1 (en) * 2002-09-26 2004-08-26 Santi Daniel V Synthetic genes
ATE368746T1 (de) * 2002-12-02 2007-08-15 Basf Ag L-rhamnose-induzierbare expressionssysteme
US8293503B2 (en) * 2003-10-03 2012-10-23 Promega Corporation Vectors for directional cloning

Similar Documents

Publication Publication Date Title
CN107012164B (zh) CRISPR/Cpf1植物基因组定向修饰功能单元、包含该功能单元的载体及其应用
CA2372464A1 (en) Isolating biological modulators from biodiverse gene fragment libraries
JP2011083296A5 (enExample)
JP2007508012A5 (enExample)
CN110607320A (zh) 一种植物基因组定向碱基编辑骨架载体及其应用
JP3844656B2 (ja) 動物細胞の形質転換のための方法
CN116286905B (zh) 牛源化CRISPR/boCas9基因编辑系统、方法及应用
US20200131504A1 (en) Plasmid library comprising two random markers and use thereof in high throughput sequencing
CN106947766A (zh) 一种枯草芽孢杆菌具有启动子功能的dna片段及其应用
DK1003888T3 (da) Nukleinsyresekvenser for polypeptider eksporteret fra mykobakterier, vektorer omfattende disse samt anvendelser til diagnosticering og forebyggelse af tuberkolose
CN116179513A (zh) 一种Cpf1蛋白及其在基因编辑中的应用
JP4768155B2 (ja) 鋳型ｄｎａの製造方法及びそれを用いた無細胞タンパク質合成系によるタンパク質の製造方法
CN112725329B (zh) 一种功能元件的建库方法及其应用
JP2008505626A5 (enExample)
CA2573022A1 (en) Novel sequence for improving expression of nucleic acid
US9803207B2 (en) Expression vector for production of recombinant proteins in prokaryotic host cells
CN106086025A (zh) 一种具有启动子功能的dna片段及其应用
CN114901820B (zh) 构建基因突变文库的方法
JP2024509048A (ja) Ｃｒｉｓｐｒ関連トランスポゾンシステム及びその使用方法
JP2024509047A (ja) Ｃｒｉｓｐｒ関連トランスポゾンシステム及びその使用方法
CN116200366A (zh) 具有XNA合成及反转录活性的Bst DNA聚合酶突变株
WO2005021719A2 (en) Libraries of recombinant chimeric proteins
CN100510070C (zh) 一种基因克隆方法
CN107287226A (zh) 一种基于Cpf1的DNA构建物以及DNA体外拼接方法
KR100428998B1 (ko) 모체 단백질과는 크기와 서열이 다른 단백질의 변이집단을생산하는 방법