CA2541177A1 - Vectors for directional cloning - Google Patents
Vectors for directional cloning Download PDFInfo
- Publication number
- CA2541177A1 CA2541177A1 CA002541177A CA2541177A CA2541177A1 CA 2541177 A1 CA2541177 A1 CA 2541177A1 CA 002541177 A CA002541177 A CA 002541177A CA 2541177 A CA2541177 A CA 2541177A CA 2541177 A1 CA2541177 A1 CA 2541177A1
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- restriction enzyme
- vector
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
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- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B50/00—ICT programming tools or database systems specially adapted for bioinformatics
- G16B50/30—Data warehousing; Computing architectures
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- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B50/00—ICT programming tools or database systems specially adapted for bioinformatics
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Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US10/678,961 US8293503B2 (en) | 2003-10-03 | 2003-10-03 | Vectors for directional cloning |
| US10/678,961 | 2003-10-03 | ||
| US10/702,228 US20050074785A1 (en) | 2003-10-03 | 2003-11-05 | Vectors for directional cloning |
| US10/702,228 | 2003-11-05 | ||
| PCT/US2004/031912 WO2005087932A2 (en) | 2003-10-03 | 2004-09-29 | Vectors for directional cloning |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2541177A1 true CA2541177A1 (en) | 2005-09-22 |
Family
ID=34971637
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002541177A Abandoned CA2541177A1 (en) | 2003-10-03 | 2004-09-29 | Vectors for directional cloning |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US20070212762A1 (enExample) |
| EP (2) | EP1685247B1 (enExample) |
| JP (1) | JP5089984B2 (enExample) |
| AT (1) | ATE442445T1 (enExample) |
| CA (1) | CA2541177A1 (enExample) |
| WO (1) | WO2005087932A2 (enExample) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8293503B2 (en) | 2003-10-03 | 2012-10-23 | Promega Corporation | Vectors for directional cloning |
| US8202727B2 (en) * | 2005-05-17 | 2012-06-19 | Ozgene Pty Ltd. | Sequential cloning system |
| AU2008212907A1 (en) * | 2007-02-05 | 2008-08-14 | Philipps-Universitat Marburg | Method of cloning at least one nucleic acid molecule of interest using type IIS restriction endonucleases, and corresponding cloning vectors, kits and system using type IIS restriction endonucleases |
| US20110244521A1 (en) | 2007-08-21 | 2011-10-06 | Takeharu Nagai | Method for preparation of recobinant dna |
| WO2009079212A2 (en) | 2007-12-03 | 2009-06-25 | Carnegie Mellon University | Linked peptide fluorogenic biosensors |
| WO2009106635A1 (en) | 2008-02-29 | 2009-09-03 | Xbrane Bioscience Aktiebolag | Expression system for proteins |
| EP2802655A4 (en) * | 2012-01-13 | 2015-11-18 | Momenta Pharmaceuticals Inc | NUCLEOTIDE CLONING METHODS |
| US10829772B2 (en) | 2012-06-22 | 2020-11-10 | Monsanto Technology Llc | Unique modular vector design |
| US11072811B2 (en) | 2013-03-15 | 2021-07-27 | Promega Corporation | Substrates for covalent tethering of proteins to functional groups or solid surfaces |
| WO2014142955A1 (en) * | 2013-03-15 | 2014-09-18 | Carnegie Mellon University | Linked peptide fluorogenic biosensors |
| CN104450919A (zh) * | 2014-12-12 | 2015-03-25 | 南华大学 | 通过插入部分摇摆寡核苷酸检测基因突变耐受能力的方法 |
| EP4159862A4 (en) * | 2020-05-26 | 2024-06-05 | Spiber Inc. | Method for preparing combinatorial library of multi-modular biosynthetic enzyme gene |
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| US4403036A (en) * | 1980-12-02 | 1983-09-06 | University Of Iowa Research Foundation | Genetic reagents for generating plasmids containing multiple copies of DNA segments |
| US4724202A (en) * | 1983-12-12 | 1988-02-09 | Molecular Diagnostics, Inc. | Use of non-hybridizable nucleic acids for the detection of nucleic acid hybridization |
| US4784949A (en) * | 1984-04-19 | 1988-11-15 | Cetus Corporation | Universal dominant selectable marker cassette |
| US4952496A (en) * | 1984-03-30 | 1990-08-28 | Associated Universities, Inc. | Cloning and expression of the gene for bacteriophage T7 RNA polymerase |
| US5693489A (en) * | 1984-03-30 | 1997-12-02 | Associated Universities, Inc. | Cloning and expression of the gene for bacteriophage T7 RNA polymerase |
| EP0178863A1 (en) * | 1984-10-15 | 1986-04-23 | Schering Corporation | Novel expression systems utilizing bacteriophage T7 promoters and gene sequences |
| US5200333A (en) * | 1985-03-01 | 1993-04-06 | New England Biolabs, Inc. | Cloning restriction and modification genes |
| DE3750708T2 (de) * | 1986-06-06 | 1995-06-29 | New England Biolabs Inc | Verfahren zur Klonierung eines Restriktionsmodifizierungssystems. |
| US5506126A (en) * | 1988-02-25 | 1996-04-09 | The General Hospital Corporation | Rapid immunoselection cloning method |
| WO1991002077A1 (en) * | 1989-07-28 | 1991-02-21 | The United States Of America, Represented By The Secretary, United States Department Of Commerce | Efficient directional genetic cloning system |
| US5122457A (en) * | 1989-10-19 | 1992-06-16 | Schering Corporation | Expression systems utilizing bacteriophage t7 promoters, gene sequences, and t7 rna polymerase |
| DE69024188T2 (de) * | 1990-01-11 | 1996-08-14 | New England Biolabs Inc | SfiI-Restriktionsendonuklease und Methylase Gene und Verfahren zu deren Klonierung. |
| US5179015A (en) * | 1990-07-23 | 1993-01-12 | New England Biolabs, Inc. | Heterospecific modification as a means to clone restriction genes |
| JP2739378B2 (ja) * | 1990-11-09 | 1998-04-15 | アメリカ合衆国 | Ｄｎａに標的を定める方法 |
| US5196330A (en) * | 1991-06-03 | 1993-03-23 | New England Biolabs, Inc. | Type ii restriction endonuclease, pme i, obtainable from pseudomonas mendocina and a process for producing the same |
| US5342782A (en) * | 1992-02-24 | 1994-08-30 | Washington University | Tightly regulated expression system for eucaryotic cells |
| BE1006085A3 (fr) * | 1992-07-31 | 1994-05-10 | Univ Bruxelles | Vecteur de clonage. |
| US6521425B2 (en) * | 1997-03-05 | 2003-02-18 | New England Biolabs, Inc. | Screening and use of reagents which block or activate intein splicing utilizing natural or homologous exteins |
| US5731411A (en) * | 1993-07-12 | 1998-03-24 | The United States Of America As Represented By The Department Of Health And Human Services | Promotion of homologous DNA pairing by RecA-derived peptides |
| US5391487A (en) * | 1993-08-13 | 1995-02-21 | Promega Corporation | Restriction endonuclease SgfI from Streptomyces griseoruber |
| US5498535A (en) * | 1994-05-24 | 1996-03-12 | New England Biolabs, Inc. | Method for direct cloning of nuclease genes in E. coli |
| DE19514310A1 (de) * | 1995-04-18 | 1996-10-24 | Univ Ludwigs Albert | Vektoren zur Transfektion von eukaryotischen Zellen, deren Verwendung und damit transfizierte Zielzellen |
| WO1997004111A1 (en) * | 1995-07-21 | 1997-02-06 | The Government Of The United States Of America, Represented By The Secretary, Department Of Health And Human Services | RecA-ASSISTED CLONING OF DNA |
| US5830690A (en) * | 1995-12-29 | 1998-11-03 | Council Of Scientific & Indus. Res. & Dept. Of Biotech. | Process for producing polypeptides |
| US5663067A (en) * | 1996-07-11 | 1997-09-02 | New England Biolabs, Inc. | Method for cloning and producing the SapI restriction endonuclease in E. coli |
| DE19653498C1 (de) * | 1996-12-20 | 1998-07-23 | Wolf M Prof Dr Bertling | Klonierungsvektor-System mit Fusionssequenz |
| US5851808A (en) * | 1997-02-28 | 1998-12-22 | Baylor College Of Medicine | Rapid subcloning using site-specific recombination |
| US6248569B1 (en) * | 1997-11-10 | 2001-06-19 | Brookhaven Science Associates | Method for introducing unidirectional nested deletions |
| US5945288A (en) * | 1997-11-24 | 1999-08-31 | New England Biolabs, Inc. | Method for cloning and producing the PmeI restriction endonuclease |
| EP1062512A4 (en) * | 1998-03-10 | 2001-12-12 | Univ California | METHODS AND TOOLS USED TO IDENTIFY COMPOUNDS THAT MODULATE ATHEROSCLEROSIS BY ALTERATION OF THE LDL-PROTEOGLYCANE BINDING |
| AU5584999A (en) * | 1998-08-28 | 2000-03-21 | Invitrogen Corporation | System for the rapid manipulation of nucleic acid sequences |
| US6096523A (en) * | 1998-11-04 | 2000-08-01 | University Of Georgia Research Foundation | Transformation vector system |
| US6184000B1 (en) * | 1999-07-23 | 2001-02-06 | The United States Of America As Represented By The Secretary Of Agriculture | System for the sequential, directional cloning of multiple DNA sequences |
| US6479280B1 (en) * | 1999-09-24 | 2002-11-12 | Vlaams Interuniversitair Institutuut Voor Biotechnologie Vzw | Recombinant phages capable of entering host cells via specific interaction with an artificial receptor |
| WO2001055371A1 (en) * | 2000-01-28 | 2001-08-02 | The Scripps Research Institute | Methods of identifying synthetic transcriptional and translational regulatory elements, and compositions relating to same |
| US6908751B2 (en) * | 2000-04-26 | 2005-06-21 | Zymogenetics, Inc. | Methods for enhancing the expression of a protein of interest by recombinant host cells |
| US20020086310A1 (en) * | 2000-09-01 | 2002-07-04 | Frank Fan | Identification of targets of antimicrobial compounds |
| EP1360490B1 (en) * | 2001-01-23 | 2011-12-21 | President and Fellows of Harvard College | Nucleic-acid programmable protein arrays |
| FR2824844B1 (fr) * | 2001-05-18 | 2003-09-19 | Genoway | Procede de clonage par double selection et vecteurs pour ce procede |
| US7094579B2 (en) * | 2002-02-13 | 2006-08-22 | Xoma Technology Ltd. | Eukaryotic signal sequences for prokaryotic expression |
| US20040166567A1 (en) * | 2002-09-26 | 2004-08-26 | Santi Daniel V | Synthetic genes |
| ATE368746T1 (de) * | 2002-12-02 | 2007-08-15 | Basf Ag | L-rhamnose-induzierbare expressionssysteme |
| US8293503B2 (en) * | 2003-10-03 | 2012-10-23 | Promega Corporation | Vectors for directional cloning |
-
2004
- 2004-09-29 EP EP04821603A patent/EP1685247B1/en not_active Expired - Lifetime
- 2004-09-29 WO PCT/US2004/031912 patent/WO2005087932A2/en not_active Ceased
- 2004-09-29 JP JP2006534051A patent/JP5089984B2/ja not_active Expired - Fee Related
- 2004-09-29 EP EP07013756A patent/EP1847611B1/en not_active Expired - Lifetime
- 2004-09-29 AT AT07013756T patent/ATE442445T1/de not_active IP Right Cessation
- 2004-09-29 CA CA002541177A patent/CA2541177A1/en not_active Abandoned
-
2006
- 2006-08-18 US US11/505,650 patent/US20070212762A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| ATE442445T1 (de) | 2009-09-15 |
| EP1847611A3 (en) | 2007-10-31 |
| EP1847611B1 (en) | 2009-09-09 |
| EP1685247B1 (en) | 2009-11-11 |
| EP1847611A2 (en) | 2007-10-24 |
| WO2005087932A2 (en) | 2005-09-22 |
| US20070212762A1 (en) | 2007-09-13 |
| WO2005087932A3 (en) | 2006-03-02 |
| EP1685247A2 (en) | 2006-08-02 |
| JP2007508012A (ja) | 2007-04-05 |
| JP5089984B2 (ja) | 2012-12-05 |
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