ATE442445T1 - Rhamnose-induzierbares expressionssystem - Google Patents

Rhamnose-induzierbares expressionssystem

Info

Publication number
ATE442445T1
ATE442445T1 AT07013756T AT07013756T ATE442445T1 AT E442445 T1 ATE442445 T1 AT E442445T1 AT 07013756 T AT07013756 T AT 07013756T AT 07013756 T AT07013756 T AT 07013756T AT E442445 T1 ATE442445 T1 AT E442445T1
Authority
AT
Austria
Prior art keywords
rhamnose
inducable
expression system
interest
host cell
Prior art date
Application number
AT07013756T
Other languages
English (en)
Inventor
Michael R Slater
Ethan Edward Strauss
Keith V Wood
James Robert Hartnett
Original Assignee
Promega Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US10/678,961 external-priority patent/US8293503B2/en
Application filed by Promega Corp filed Critical Promega Corp
Application granted granted Critical
Publication of ATE442445T1 publication Critical patent/ATE442445T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B50/00—ICT programming tools or database systems specially adapted for bioinformatics
    • G16B50/30—Data warehousing; Computing architectures
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B50/00—ICT programming tools or database systems specially adapted for bioinformatics

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Theoretical Computer Science (AREA)
  • Medical Informatics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Bioethics (AREA)
  • Databases & Information Systems (AREA)
  • Evolutionary Biology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Optical Communication System (AREA)
AT07013756T 2003-10-03 2004-09-29 Rhamnose-induzierbares expressionssystem ATE442445T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/678,961 US8293503B2 (en) 2003-10-03 2003-10-03 Vectors for directional cloning
US10/702,228 US20050074785A1 (en) 2003-10-03 2003-11-05 Vectors for directional cloning

Publications (1)

Publication Number Publication Date
ATE442445T1 true ATE442445T1 (de) 2009-09-15

Family

ID=34971637

Family Applications (1)

Application Number Title Priority Date Filing Date
AT07013756T ATE442445T1 (de) 2003-10-03 2004-09-29 Rhamnose-induzierbares expressionssystem

Country Status (6)

Country Link
US (1) US20070212762A1 (de)
EP (2) EP1847611B1 (de)
JP (1) JP5089984B2 (de)
AT (1) ATE442445T1 (de)
CA (1) CA2541177A1 (de)
WO (1) WO2005087932A2 (de)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8293503B2 (en) 2003-10-03 2012-10-23 Promega Corporation Vectors for directional cloning
AU2006246975B2 (en) * 2005-05-17 2011-08-11 Ozgene Pty Ltd Sequential cloning system
WO2008095927A1 (en) * 2007-02-05 2008-08-14 Philipps-Universität Marburg Method of cloning at least one nucleic acid molecule of interest using type iis restriction endonucleases, and corresponding cloning vectors, kits and system using type iis restriction endonucleases
WO2009025209A1 (ja) 2007-08-21 2009-02-26 National University Corporation Hokkaido University 組換えｄｎａの調製方法
US8426153B2 (en) 2007-12-03 2013-04-23 Carnegie Mellon University Linked peptides fluorogenic biosensors
ATE544860T1 (de) 2008-02-29 2012-02-15 Xbrane Bioscience Aktiebolag Expressionssystem für proteine
US20140363853A1 (en) * 2012-01-13 2014-12-11 Momenta Pharmaceuticals. Inc, Nucleotide cloning methods
US10829772B2 (en) 2012-06-22 2020-11-10 Monsanto Technology Llc Unique modular vector design
US11072811B2 (en) 2013-03-15 2021-07-27 Promega Corporation Substrates for covalent tethering of proteins to functional groups or solid surfaces
US20160033490A1 (en) * 2013-03-15 2016-02-04 Carnegie Mellon University Linked Peptide Fluorogenic Biosensors
CN104450919A (zh) * 2014-12-12 2015-03-25 南华大学 通过插入部分摇摆寡核苷酸检测基因突变耐受能力的方法
EP4159862A1 (de) * 2020-05-26 2023-04-05 Spiber Inc. Verfahren zur herstellung einer kombinatorischen bibliothek eines multimodularen biosynthetischen enzymgens

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US4403036A (en) * 1980-12-02 1983-09-06 University Of Iowa Research Foundation Genetic reagents for generating plasmids containing multiple copies of DNA segments
US4724202A (en) * 1983-12-12 1988-02-09 Molecular Diagnostics, Inc. Use of non-hybridizable nucleic acids for the detection of nucleic acid hybridization
US4784949A (en) * 1984-04-19 1988-11-15 Cetus Corporation Universal dominant selectable marker cassette
US4952496A (en) * 1984-03-30 1990-08-28 Associated Universities, Inc. Cloning and expression of the gene for bacteriophage T7 RNA polymerase
US5693489A (en) * 1984-03-30 1997-12-02 Associated Universities, Inc. Cloning and expression of the gene for bacteriophage T7 RNA polymerase
EP0178863A1 (de) * 1984-10-15 1986-04-23 Schering Corporation Expressionssysteme die sich der Bakteriophage-T7-Promotoren bedienen und Gensequenzen
US5200333A (en) * 1985-03-01 1993-04-06 New England Biolabs, Inc. Cloning restriction and modification genes
DE3750708T2 (de) * 1986-06-06 1995-06-29 New England Biolabs Inc Verfahren zur Klonierung eines Restriktionsmodifizierungssystems.
US5506126A (en) * 1988-02-25 1996-04-09 The General Hospital Corporation Rapid immunoselection cloning method
EP0484440A4 (en) * 1989-07-28 1993-01-07 Us Health Efficient directional genetic cloning system
US5122457A (en) * 1989-10-19 1992-06-16 Schering Corporation Expression systems utilizing bacteriophage t7 promoters, gene sequences, and t7 rna polymerase
EP0437100B1 (de) * 1990-01-11 1995-12-13 New England Biolabs, Inc. SfiI-Restriktionsendonuklease und Methylase Gene und Verfahren zu deren Klonierung.
US5179015A (en) * 1990-07-23 1993-01-12 New England Biolabs, Inc. Heterospecific modification as a means to clone restriction genes
WO1992008791A1 (en) * 1990-11-09 1992-05-29 The United States Of America, As Represented By The Secretary, U.S. Department Of Commerce Method of targeting dna
US5196330A (en) * 1991-06-03 1993-03-23 New England Biolabs, Inc. Type ii restriction endonuclease, pme i, obtainable from pseudomonas mendocina and a process for producing the same
US5342782A (en) * 1992-02-24 1994-08-30 Washington University Tightly regulated expression system for eucaryotic cells
BE1006085A3 (fr) * 1992-07-31 1994-05-10 Univ Bruxelles Vecteur de clonage.
US6521425B2 (en) * 1997-03-05 2003-02-18 New England Biolabs, Inc. Screening and use of reagents which block or activate intein splicing utilizing natural or homologous exteins
US5731411A (en) * 1993-07-12 1998-03-24 The United States Of America As Represented By The Department Of Health And Human Services Promotion of homologous DNA pairing by RecA-derived peptides
US5391487A (en) * 1993-08-13 1995-02-21 Promega Corporation Restriction endonuclease SgfI from Streptomyces griseoruber
US5498535A (en) * 1994-05-24 1996-03-12 New England Biolabs, Inc. Method for direct cloning of nuclease genes in E. coli
DE19514310A1 (de) * 1995-04-18 1996-10-24 Univ Ludwigs Albert Vektoren zur Transfektion von eukaryotischen Zellen, deren Verwendung und damit transfizierte Zielzellen
CA2227413A1 (en) * 1995-07-21 1997-02-06 The Government Of The United States, Represented By The Secretary, Depar Tment Of Health And Human Services Reca-assisted cloning of dna
US5830690A (en) * 1995-12-29 1998-11-03 Council Of Scientific & Indus. Res. & Dept. Of Biotech. Process for producing polypeptides
US5663067A (en) * 1996-07-11 1997-09-02 New England Biolabs, Inc. Method for cloning and producing the SapI restriction endonuclease in E. coli
DE19653498C1 (de) * 1996-12-20 1998-07-23 Wolf M Prof Dr Bertling Klonierungsvektor-System mit Fusionssequenz
US5851808A (en) * 1997-02-28 1998-12-22 Baylor College Of Medicine Rapid subcloning using site-specific recombination
US6248569B1 (en) * 1997-11-10 2001-06-19 Brookhaven Science Associates Method for introducing unidirectional nested deletions
US5945288A (en) * 1997-11-24 1999-08-31 New England Biolabs, Inc. Method for cloning and producing the PmeI restriction endonuclease
CA2323056A1 (en) * 1998-03-10 1999-09-16 Regents Of The University Of California Methods and tools for identifying compounds which modulate atherosclerosis by impacting ldl-proteoglycan binding
EP1114148A4 (de) * 1998-08-28 2004-12-22 Invitrogen Corp System zur schnellen manipulation von nukleinsäuresequenzen.
US6096523A (en) * 1998-11-04 2000-08-01 University Of Georgia Research Foundation Transformation vector system
US6184000B1 (en) * 1999-07-23 2001-02-06 The United States Of America As Represented By The Secretary Of Agriculture System for the sequential, directional cloning of multiple DNA sequences
US6479280B1 (en) * 1999-09-24 2002-11-12 Vlaams Interuniversitair Institutuut Voor Biotechnologie Vzw Recombinant phages capable of entering host cells via specific interaction with an artificial receptor
EP1259602A1 (de) * 2000-01-28 2002-11-27 The Scripps Research Institute Methoden zur identifizierung von regulatorischen elementen der transkription und translation und damit verwandte verbindungen
US6908751B2 (en) * 2000-04-26 2005-06-21 Zymogenetics, Inc. Methods for enhancing the expression of a protein of interest by recombinant host cells
US20020086310A1 (en) * 2000-09-01 2002-07-04 Frank Fan Identification of targets of antimicrobial compounds
CA2434139C (en) * 2001-01-23 2014-05-27 President And Fellows Of Harvard College Nucleic-acid programmable protein arrays
FR2824844B1 (fr) * 2001-05-18 2003-09-19 Genoway Procede de clonage par double selection et vecteurs pour ce procede
US7094579B2 (en) * 2002-02-13 2006-08-22 Xoma Technology Ltd. Eukaryotic signal sequences for prokaryotic expression
EP1576140A4 (de) * 2002-09-26 2007-08-08 Kosan Biosciences Inc Synthetische gene
AU2003288189B8 (en) * 2002-12-02 2009-06-25 Basf Aktiengesellschaft L-rhamnose-inducible expression systems
US8293503B2 (en) * 2003-10-03 2012-10-23 Promega Corporation Vectors for directional cloning

Also Published As

Publication number Publication date
EP1685247A2 (de) 2006-08-02
US20070212762A1 (en) 2007-09-13
JP2007508012A (ja) 2007-04-05
EP1685247B1 (de) 2009-11-11
WO2005087932A2 (en) 2005-09-22
EP1847611A2 (de) 2007-10-24
JP5089984B2 (ja) 2012-12-05
EP1847611B1 (de) 2009-09-09
WO2005087932A3 (en) 2006-03-02
CA2541177A1 (en) 2005-09-22
EP1847611A3 (de) 2007-10-31

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