ATE442445T1 - Rhamnose-induzierbares expressionssystem - Google Patents

Rhamnose-induzierbares expressionssystem

Info

Publication number
ATE442445T1
ATE442445T1 AT07013756T AT07013756T ATE442445T1 AT E442445 T1 ATE442445 T1 AT E442445T1 AT 07013756 T AT07013756 T AT 07013756T AT 07013756 T AT07013756 T AT 07013756T AT E442445 T1 ATE442445 T1 AT E442445T1
Authority
AT
Austria
Prior art keywords
rhamnose
inducable
expression system
interest
host cell
Prior art date
Application number
AT07013756T
Other languages
English (en)
Inventor
Michael R Slater
Ethan Edward Strauss
Keith V Wood
James Robert Hartnett
Original Assignee
Promega Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US10/678,961 external-priority patent/US8293503B2/en
Application filed by Promega Corp filed Critical Promega Corp
Application granted granted Critical
Publication of ATE442445T1 publication Critical patent/ATE442445T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B50/00—ICT programming tools or database systems specially adapted for bioinformatics
    • G16B50/30—Data warehousing; Computing architectures
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B50/00—ICT programming tools or database systems specially adapted for bioinformatics

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Theoretical Computer Science (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Databases & Information Systems (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Bioethics (AREA)
  • Evolutionary Biology (AREA)
  • Medical Informatics (AREA)
  • Cell Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Optical Communication System (AREA)
AT07013756T 2003-10-03 2004-09-29 Rhamnose-induzierbares expressionssystem ATE442445T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/678,961 US8293503B2 (en) 2003-10-03 2003-10-03 Vectors for directional cloning
US10/702,228 US20050074785A1 (en) 2003-10-03 2003-11-05 Vectors for directional cloning

Publications (1)

Publication Number Publication Date
ATE442445T1 true ATE442445T1 (de) 2009-09-15

Family

ID=34971637

Family Applications (1)

Application Number Title Priority Date Filing Date
AT07013756T ATE442445T1 (de) 2003-10-03 2004-09-29 Rhamnose-induzierbares expressionssystem

Country Status (6)

Country Link
US (1) US20070212762A1 (de)
EP (2) EP1685247B1 (de)
JP (1) JP5089984B2 (de)
AT (1) ATE442445T1 (de)
CA (1) CA2541177A1 (de)
WO (1) WO2005087932A2 (de)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8293503B2 (en) * 2003-10-03 2012-10-23 Promega Corporation Vectors for directional cloning
CA2608636C (en) * 2005-05-17 2015-02-10 Frank Koentgen Sequential cloning system
JPWO2009025209A1 (ja) 2007-08-21 2010-11-25 国立大学法人北海道大学 組換えｄｎａの調製方法
US20100291633A1 (en) * 2007-09-03 2010-11-18 Thorsten Selmer Method of cloning at least one nucleic acid molecule of interest using type iis restriction endonucleases, and corresponding cloning vectors, kits and system using type iis restriction endonucleases
US8426153B2 (en) 2007-12-03 2013-04-23 Carnegie Mellon University Linked peptides fluorogenic biosensors
ES2381433T3 (es) 2008-02-29 2012-05-28 Xbrane Bioscience Aktiebolag Sistema de expresión para proteínas
US20140363853A1 (en) * 2012-01-13 2014-12-11 Momenta Pharmaceuticals. Inc, Nucleotide cloning methods
WO2013191950A2 (en) 2012-06-22 2013-12-27 Monsanto Technology Llc Unique modular vector design
US20160033490A1 (en) * 2013-03-15 2016-02-04 Carnegie Mellon University Linked Peptide Fluorogenic Biosensors
EP2969435B1 (de) 2013-03-15 2021-11-03 Promega Corporation Substrate für kovalentes binden von proteinen an funktionelle gruppen oder feste oberflächen
CN104450919A (zh) * 2014-12-12 2015-03-25 南华大学 通过插入部分摇摆寡核苷酸检测基因突变耐受能力的方法
EP4159862A4 (de) * 2020-05-26 2024-06-05 Spiber Inc. Verfahren zur herstellung einer kombinatorischen bibliothek eines multimodularen biosynthetischen enzymgens

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US4724202A (en) * 1983-12-12 1988-02-09 Molecular Diagnostics, Inc. Use of non-hybridizable nucleic acids for the detection of nucleic acid hybridization
US4784949A (en) * 1984-04-19 1988-11-15 Cetus Corporation Universal dominant selectable marker cassette
US4952496A (en) * 1984-03-30 1990-08-28 Associated Universities, Inc. Cloning and expression of the gene for bacteriophage T7 RNA polymerase
US5693489A (en) * 1984-03-30 1997-12-02 Associated Universities, Inc. Cloning and expression of the gene for bacteriophage T7 RNA polymerase
EP0178863A1 (de) * 1984-10-15 1986-04-23 Schering Corporation Expressionssysteme die sich der Bakteriophage-T7-Promotoren bedienen und Gensequenzen
US5200333A (en) * 1985-03-01 1993-04-06 New England Biolabs, Inc. Cloning restriction and modification genes
DE3751923T2 (de) * 1986-06-06 1997-04-03 New England Biolabs Inc Verfahren zur Klonierung von Restriktions-Modifikationssystemen
US5506126A (en) * 1988-02-25 1996-04-09 The General Hospital Corporation Rapid immunoselection cloning method
JPH04503309A (ja) * 1989-07-28 1992-06-18 アメリカ合衆国 効果的な方向性遺伝子クローニング系
US5122457A (en) * 1989-10-19 1992-06-16 Schering Corporation Expression systems utilizing bacteriophage t7 promoters, gene sequences, and t7 rna polymerase
EP0437100B1 (de) * 1990-01-11 1995-12-13 New England Biolabs, Inc. SfiI-Restriktionsendonuklease und Methylase Gene und Verfahren zu deren Klonierung.
US5179015A (en) * 1990-07-23 1993-01-12 New England Biolabs, Inc. Heterospecific modification as a means to clone restriction genes
CA2095624C (en) * 1990-11-09 2000-06-13 Rafael D. Camerini-Otero Method of targeting dna
US5196330A (en) * 1991-06-03 1993-03-23 New England Biolabs, Inc. Type ii restriction endonuclease, pme i, obtainable from pseudomonas mendocina and a process for producing the same
US5342782A (en) * 1992-02-24 1994-08-30 Washington University Tightly regulated expression system for eucaryotic cells
BE1006085A3 (fr) * 1992-07-31 1994-05-10 Univ Bruxelles Vecteur de clonage.
US6521425B2 (en) * 1997-03-05 2003-02-18 New England Biolabs, Inc. Screening and use of reagents which block or activate intein splicing utilizing natural or homologous exteins
US5731411A (en) * 1993-07-12 1998-03-24 The United States Of America As Represented By The Department Of Health And Human Services Promotion of homologous DNA pairing by RecA-derived peptides
US5391487A (en) * 1993-08-13 1995-02-21 Promega Corporation Restriction endonuclease SgfI from Streptomyces griseoruber
US5498535A (en) * 1994-05-24 1996-03-12 New England Biolabs, Inc. Method for direct cloning of nuclease genes in E. coli
DE19514310A1 (de) * 1995-04-18 1996-10-24 Univ Ludwigs Albert Vektoren zur Transfektion von eukaryotischen Zellen, deren Verwendung und damit transfizierte Zielzellen
JPH11509423A (ja) * 1995-07-21 1999-08-24 ザ ガヴァメント オヴ ザ ユナイテッド ステイツ オヴ アメリカ、リプレゼンティッド バイ ザ セクレタリー、ディパートメント オヴ ヘルス アンド ヒューマン サーヴィスィーズ Ｒｅｃ支援ＤＮＡクローニング
US5830690A (en) * 1995-12-29 1998-11-03 Council Of Scientific & Indus. Res. & Dept. Of Biotech. Process for producing polypeptides
US5663067A (en) * 1996-07-11 1997-09-02 New England Biolabs, Inc. Method for cloning and producing the SapI restriction endonuclease in E. coli
DE19653498C1 (de) * 1996-12-20 1998-07-23 Wolf M Prof Dr Bertling Klonierungsvektor-System mit Fusionssequenz
US5851808A (en) * 1997-02-28 1998-12-22 Baylor College Of Medicine Rapid subcloning using site-specific recombination
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EP1062512A4 (de) * 1998-03-10 2001-12-12 Univ California Verfahren und hilfsmittel zum identifizieren von verbindungen, die atherosklerose modulieren indem sie ldl-proteoglykanbindungen beeinflussen
AU5584999A (en) * 1998-08-28 2000-03-21 Invitrogen Corporation System for the rapid manipulation of nucleic acid sequences
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US6184000B1 (en) * 1999-07-23 2001-02-06 The United States Of America As Represented By The Secretary Of Agriculture System for the sequential, directional cloning of multiple DNA sequences
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EP1274838A1 (de) * 2000-01-28 2003-01-15 The Scripps Research Institute Synthetische ribosomale eintrittsseiten und methoden um diese zu identifizieren
US6908751B2 (en) * 2000-04-26 2005-06-21 Zymogenetics, Inc. Methods for enhancing the expression of a protein of interest by recombinant host cells
US20020086310A1 (en) * 2000-09-01 2002-07-04 Frank Fan Identification of targets of antimicrobial compounds
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FR2824844B1 (fr) * 2001-05-18 2003-09-19 Genoway Procede de clonage par double selection et vecteurs pour ce procede
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ES2288630T3 (es) * 2002-12-02 2008-01-16 Basf Aktiengesellschaft Sistema de expresion, que puede ser inducido por medio de la l-ramnosa.
US8293503B2 (en) * 2003-10-03 2012-10-23 Promega Corporation Vectors for directional cloning

Also Published As

Publication number Publication date
EP1685247B1 (de) 2009-11-11
CA2541177A1 (en) 2005-09-22
US20070212762A1 (en) 2007-09-13
EP1847611A2 (de) 2007-10-24
EP1847611B1 (de) 2009-09-09
JP2007508012A (ja) 2007-04-05
JP5089984B2 (ja) 2012-12-05
EP1685247A2 (de) 2006-08-02
EP1847611A3 (de) 2007-10-31
WO2005087932A2 (en) 2005-09-22
WO2005087932A3 (en) 2006-03-02

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