JP2010528618A5 - - Google Patents
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- JP2010528618A5 JP2010528618A5 JP2010510514A JP2010510514A JP2010528618A5 JP 2010528618 A5 JP2010528618 A5 JP 2010528618A5 JP 2010510514 A JP2010510514 A JP 2010510514A JP 2010510514 A JP2010510514 A JP 2010510514A JP 2010528618 A5 JP2010528618 A5 JP 2010528618A5
- Authority
- JP
- Japan
- Prior art keywords
- probe
- ligand
- receptor
- nucleic acid
- target nucleic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- 239000000523 sample Substances 0.000 claims description 46
- 108090001007 Interleukin-8 Proteins 0.000 claims description 31
- 238000000034 method Methods 0.000 claims description 14
- 238000001514 detection method Methods 0.000 claims description 12
- 239000003446 ligand Substances 0.000 claims 13
- 102000039446 nucleic acids Human genes 0.000 claims 11
- 108020004707 nucleic acids Proteins 0.000 claims 11
- 150000007523 nucleic acids Chemical class 0.000 claims 11
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims 4
- 239000000758 substrate Substances 0.000 claims 4
- 102000040430 polynucleotide Human genes 0.000 claims 3
- 108091033319 polynucleotide Proteins 0.000 claims 3
- 239000002157 polynucleotide Substances 0.000 claims 3
- 102000002260 Alkaline Phosphatase Human genes 0.000 claims 2
- 108020004774 Alkaline Phosphatase Proteins 0.000 claims 2
- 108010015776 Glucose oxidase Proteins 0.000 claims 2
- 239000004366 Glucose oxidase Substances 0.000 claims 2
- 108010029541 Laccase Proteins 0.000 claims 2
- 102000003992 Peroxidases Human genes 0.000 claims 2
- 108010090804 Streptavidin Proteins 0.000 claims 2
- 108010046334 Urease Proteins 0.000 claims 2
- 229960002685 biotin Drugs 0.000 claims 2
- 235000020958 biotin Nutrition 0.000 claims 2
- 239000011616 biotin Substances 0.000 claims 2
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 claims 2
- 229940116332 glucose oxidase Drugs 0.000 claims 2
- 235000019420 glucose oxidase Nutrition 0.000 claims 2
- 108040007629 peroxidase activity proteins Proteins 0.000 claims 2
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 18
- 238000002474 experimental method Methods 0.000 description 5
- 210000003296 saliva Anatomy 0.000 description 5
- 238000000338 in vitro Methods 0.000 description 4
- 238000003757 reverse transcription PCR Methods 0.000 description 4
- 239000013256 coordination polymer Substances 0.000 description 3
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 2
- 239000013614 RNA sample Substances 0.000 description 2
- 238000011529 RT qPCR Methods 0.000 description 2
- 208000000102 Squamous Cell Carcinoma of Head and Neck Diseases 0.000 description 2
- 239000002299 complementary DNA Substances 0.000 description 2
- 238000000835 electrochemical detection Methods 0.000 description 2
- 108020004999 messenger RNA Proteins 0.000 description 2
- 201000002740 oral squamous cell carcinoma Diseases 0.000 description 2
- 238000010839 reverse transcription Methods 0.000 description 2
- 238000013518 transcription Methods 0.000 description 2
- 230000035897 transcription Effects 0.000 description 2
- 241000945470 Arcturus Species 0.000 description 1
- 108020004414 DNA Proteins 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- 102100034343 Integrase Human genes 0.000 description 1
- 108091034117 Oligonucleotide Proteins 0.000 description 1
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
- 229960005542 ethidium bromide Drugs 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 238000012417 linear regression Methods 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 239000003161 ribonuclease inhibitor Substances 0.000 description 1
- 238000004611 spectroscopical analysis Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US94105707P | 2007-05-31 | 2007-05-31 | |
| US60/941,057 | 2007-05-31 | ||
| PCT/US2008/065286 WO2009017878A2 (en) | 2007-05-31 | 2008-05-30 | High specificity and high sensitivity detection based on steric hindrance & enzyme-related signal amplification |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2010528618A JP2010528618A (ja) | 2010-08-26 |
| JP2010528618A5 true JP2010528618A5 (https=) | 2011-10-06 |
| JP5389789B2 JP5389789B2 (ja) | 2014-01-15 |
Family
ID=40305157
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2010510514A Expired - Fee Related JP5389789B2 (ja) | 2007-05-31 | 2008-05-30 | 立体障害および酵素に関連するシグナル増幅に基づく高特異性かつ高感度の検出 |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US20100248231A1 (https=) |
| EP (1) | EP2158482B1 (https=) |
| JP (1) | JP5389789B2 (https=) |
| KR (1) | KR20100038330A (https=) |
| CN (1) | CN101849185A (https=) |
| CA (1) | CA2688155C (https=) |
| WO (1) | WO2009017878A2 (https=) |
Families Citing this family (61)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11111543B2 (en) | 2005-07-29 | 2021-09-07 | Natera, Inc. | System and method for cleaning noisy genetic data and determining chromosome copy number |
| US8532930B2 (en) | 2005-11-26 | 2013-09-10 | Natera, Inc. | Method for determining the number of copies of a chromosome in the genome of a target individual using genetic data from genetically related individuals |
| US9424392B2 (en) | 2005-11-26 | 2016-08-23 | Natera, Inc. | System and method for cleaning noisy genetic data from target individuals using genetic data from genetically related individuals |
| US10083273B2 (en) | 2005-07-29 | 2018-09-25 | Natera, Inc. | System and method for cleaning noisy genetic data and determining chromosome copy number |
| US11111544B2 (en) | 2005-07-29 | 2021-09-07 | Natera, Inc. | System and method for cleaning noisy genetic data and determining chromosome copy number |
| US10081839B2 (en) | 2005-07-29 | 2018-09-25 | Natera, Inc | System and method for cleaning noisy genetic data and determining chromosome copy number |
| CN102171565B (zh) | 2008-08-04 | 2015-04-29 | 纳特拉公司 | 等位基因调用和倍性调用的方法 |
| JP2011067174A (ja) * | 2009-09-28 | 2011-04-07 | Fujitsu Ltd | 標的検出装置及びその製造方法 |
| US20120185176A1 (en) | 2009-09-30 | 2012-07-19 | Natera, Inc. | Methods for Non-Invasive Prenatal Ploidy Calling |
| US12152275B2 (en) | 2010-05-18 | 2024-11-26 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
| US11332793B2 (en) | 2010-05-18 | 2022-05-17 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| US11408031B2 (en) | 2010-05-18 | 2022-08-09 | Natera, Inc. | Methods for non-invasive prenatal paternity testing |
| EP2572003A4 (en) | 2010-05-18 | 2016-01-13 | Natera Inc | METHOD FOR NONINVASIVE PRANATAL PLOIDIE ASSIGNMENT |
| US20190010543A1 (en) | 2010-05-18 | 2019-01-10 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| US11939634B2 (en) | 2010-05-18 | 2024-03-26 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| US11326208B2 (en) | 2010-05-18 | 2022-05-10 | Natera, Inc. | Methods for nested PCR amplification of cell-free DNA |
| US10316362B2 (en) | 2010-05-18 | 2019-06-11 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| US12545960B2 (en) | 2010-05-18 | 2026-02-10 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| US11322224B2 (en) | 2010-05-18 | 2022-05-03 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
| US11339429B2 (en) | 2010-05-18 | 2022-05-24 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
| US12221653B2 (en) | 2010-05-18 | 2025-02-11 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| US11332785B2 (en) | 2010-05-18 | 2022-05-17 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
| US9677118B2 (en) | 2014-04-21 | 2017-06-13 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| WO2012088456A2 (en) | 2010-12-22 | 2012-06-28 | Natera, Inc. | Methods for non-invasive prenatal paternity testing |
| AU2011358564B9 (en) | 2011-02-09 | 2017-07-13 | Natera, Inc | Methods for non-invasive prenatal ploidy calling |
| EP2715351B1 (en) * | 2011-05-24 | 2016-10-19 | The Regents of The University of California | Method for exosomal biomarker detection by electric field-induced release and measurement |
| US9335292B2 (en) | 2011-10-13 | 2016-05-10 | Auburn University | Electrochemical proximity assay |
| EP2831587B1 (en) | 2012-03-27 | 2018-05-16 | Ventana Medical Systems, Inc. | Signaling conjugates and methods of use |
| WO2013153911A1 (ja) * | 2012-04-12 | 2013-10-17 | 国立大学法人東京大学 | 核酸の定量方法、検出プローブ、検出プローブセット、及び核酸の検出方法 |
| US20140100126A1 (en) | 2012-08-17 | 2014-04-10 | Natera, Inc. | Method for Non-Invasive Prenatal Testing Using Parental Mosaicism Data |
| US10262755B2 (en) | 2014-04-21 | 2019-04-16 | Natera, Inc. | Detecting cancer mutations and aneuploidy in chromosomal segments |
| US9499870B2 (en) | 2013-09-27 | 2016-11-22 | Natera, Inc. | Cell free DNA diagnostic testing standards |
| US10577655B2 (en) | 2013-09-27 | 2020-03-03 | Natera, Inc. | Cell free DNA diagnostic testing standards |
| US12492429B2 (en) | 2014-04-21 | 2025-12-09 | Natera, Inc. | Detecting mutations and ploidy in chromosomal segments |
| EP3957749A1 (en) | 2014-04-21 | 2022-02-23 | Natera, Inc. | Detecting tumour specific mutations in biopsies with whole exome sequencing and in cell-free samples |
| US20180173845A1 (en) | 2014-06-05 | 2018-06-21 | Natera, Inc. | Systems and Methods for Detection of Aneuploidy |
| WO2016183106A1 (en) | 2015-05-11 | 2016-11-17 | Natera, Inc. | Methods and compositions for determining ploidy |
| ES2753274T5 (es) * | 2015-08-21 | 2023-05-26 | 42 Life Sciences Gmbh & Co Kg | Composición y procedimiento de hibridación |
| WO2017181202A2 (en) | 2016-04-15 | 2017-10-19 | Natera, Inc. | Methods for lung cancer detection |
| US11485996B2 (en) | 2016-10-04 | 2022-11-01 | Natera, Inc. | Methods for characterizing copy number variation using proximity-litigation sequencing |
| GB201618485D0 (en) | 2016-11-02 | 2016-12-14 | Ucl Business Plc | Method of detecting tumour recurrence |
| US10011870B2 (en) | 2016-12-07 | 2018-07-03 | Natera, Inc. | Compositions and methods for identifying nucleic acid molecules |
| US10894976B2 (en) | 2017-02-21 | 2021-01-19 | Natera, Inc. | Compositions, methods, and kits for isolating nucleic acids |
| US10852274B2 (en) | 2017-03-09 | 2020-12-01 | Auburn University | Differential circuit for background correction in electrochemical measurements |
| US11505799B2 (en) | 2017-07-07 | 2022-11-22 | Innamed, Inc. | Aptamers for measuring lipoprotein levels |
| CA3074349A1 (en) * | 2017-09-29 | 2019-04-04 | Boehringer Ingelheim Vetmedica Gmbh | Sensor apparatus and method for testing a sample |
| JP2021506342A (ja) | 2017-12-14 | 2021-02-22 | ティーエーアイ ダイアグノスティックス インコーポレイテッドＴａｉ Ｄｉａｇｎｏｓｔｉｃｓ，Ｉｎｃ． | 移植のための移植片適合性の評価 |
| CN108444992B (zh) * | 2018-02-09 | 2020-11-13 | 河南工业大学 | 一种黄曲霉毒素定量检测试剂盒及其检测方法 |
| US12398389B2 (en) | 2018-02-15 | 2025-08-26 | Natera, Inc. | Methods for isolating nucleic acids with size selection |
| US10871485B2 (en) | 2018-04-13 | 2020-12-22 | Rarecyte, Inc. | Kits for labeling of biomarkers and methods of using the same |
| CA3090426A1 (en) | 2018-04-14 | 2019-10-17 | Natera, Inc. | Methods for cancer detection and monitoring by means of personalized detection of circulating tumor dna |
| US11560565B2 (en) | 2018-06-13 | 2023-01-24 | Auburn University | Electrochemical detection nanostructure, systems, and uses thereof |
| US12234509B2 (en) | 2018-07-03 | 2025-02-25 | Natera, Inc. | Methods for detection of donor-derived cell-free DNA |
| CN109406596B (zh) * | 2018-12-13 | 2021-01-01 | 合肥工业大学 | 一种检测microRNA-21的电化学传感器、其制备方法及应用 |
| EP3980559A1 (en) | 2019-06-06 | 2022-04-13 | Natera, Inc. | Methods for detecting immune cell dna and monitoring immune system |
| BE1028157B9 (fr) * | 2020-04-07 | 2022-07-11 | Vaccim | Dispositif et procédé pour déterminer, au départ d'un échantillon préalablement prélevé chez un individu, qu'au moins un anticorps cible a été ou est présent chez ledit individu |
| CN113466445B (zh) * | 2021-06-30 | 2023-06-30 | 陕西师范大学 | 基于杂交链式-酶显色反应检测Hg2+和Ag+的生物传感器及其制备方法和应用 |
| CN115112883B (zh) * | 2022-06-29 | 2025-05-09 | 深圳市德大科技有限公司 | 一种miR-Chr8:96检测试剂盒及其检测方法 |
| JPWO2024157925A1 (https=) * | 2023-01-26 | 2024-08-02 | ||
| CN116626120B (zh) * | 2023-04-25 | 2024-12-13 | 苏州中科苏净生物技术有限公司 | 一种基于RPA与CRISPR/Cas的电化学核酸检测传感元件及检测方法 |
| CN116660345B (zh) * | 2023-04-25 | 2024-09-06 | 苏州中科苏净生物技术有限公司 | 一种基于CRISPR/Cas的电化学核酸检测传感元件及检测方法 |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5925517A (en) * | 1993-11-12 | 1999-07-20 | The Public Health Research Institute Of The City Of New York, Inc. | Detectably labeled dual conformation oligonucleotide probes, assays and kits |
| US7432048B2 (en) * | 1996-11-29 | 2008-10-07 | Third Wave Technologies, Inc. | Reactions on a solid surface |
| US6287765B1 (en) * | 1998-05-20 | 2001-09-11 | Molecular Machines, Inc. | Methods for detecting and identifying single molecules |
| US7118860B2 (en) * | 1999-10-29 | 2006-10-10 | Stratagene California | Methods for detection of a target nucleic acid by capture |
| US7291457B2 (en) * | 2001-06-21 | 2007-11-06 | The Regents Of The University Of California | Electrochemical detection of mismatch nucleic acids |
| WO2003000933A1 (en) * | 2001-06-25 | 2003-01-03 | Georgia Tech Research Corporation | Dual resonance energy transfer nucleic acid probes |
| US20030096781A1 (en) * | 2001-08-31 | 2003-05-22 | University Of Southern California | IL-8 is an autocrine growth factor and a surrogate marker for Kaposi's sarcoma |
| FR2834521B1 (fr) * | 2002-01-10 | 2004-12-17 | Bio Merieux | Procede de detection et/ou d'identification de l'espece animale d'origine de la matiere animale contenue dans un echantillon |
| DE10220935B3 (de) * | 2002-05-10 | 2004-02-05 | Siemens Ag | Verfahren für die biochemische Analytik von DNA und zugehörige Anordnung |
| WO2005001129A2 (en) * | 2003-06-06 | 2005-01-06 | Applera Corporation | Mobility cassettes |
| WO2007033283A2 (en) * | 2005-09-13 | 2007-03-22 | University Of Hawaii | Disposable electrode system for detection of selected nucleic acid sequences |
-
2008
- 2008-05-30 CN CN200880101316A patent/CN101849185A/zh active Pending
- 2008-05-30 CA CA2688155A patent/CA2688155C/en active Active
- 2008-05-30 US US12/601,774 patent/US20100248231A1/en not_active Abandoned
- 2008-05-30 JP JP2010510514A patent/JP5389789B2/ja not_active Expired - Fee Related
- 2008-05-30 WO PCT/US2008/065286 patent/WO2009017878A2/en not_active Ceased
- 2008-05-30 KR KR1020097027652A patent/KR20100038330A/ko not_active Ceased
- 2008-05-30 EP EP08826854.5A patent/EP2158482B1/en active Active
-
2012
- 2012-05-02 US US13/462,709 patent/US20130115594A1/en not_active Abandoned
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