CN101849185A - 基于空间位阻和酶相关信号放大的高特异性和高灵敏度检测 - Google Patents

基于空间位阻和酶相关信号放大的高特异性和高灵敏度检测 Download PDF

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CN101849185A
CN101849185A CN200880101316A CN200880101316A CN101849185A CN 101849185 A CN101849185 A CN 101849185A CN 200880101316 A CN200880101316 A CN 200880101316A CN 200880101316 A CN200880101316 A CN 200880101316A CN 101849185 A CN101849185 A CN 101849185A
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probe
signal
target
nucleic acid
receptor
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魏芳
B·G·齐默尔曼
D·T·W·王
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University of California San Diego UCSD
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
    • C—CHEMISTRY; METALLURGY
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30—Oligonucleotides characterised by their secondary structure
    • C12Q2525/301—Hairpin oligonucleotides
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    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/131—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a member of a cognate binding pair, i.e. extends to antibodies, haptens, avidin
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    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/10—Detection mode being characterised by the assay principle
    • C12Q2565/102—Multiple non-interacting labels
    • C12Q2565/1025—Multiple non-interacting labels labels being on the same oligonucleotide
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    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
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    • C12Q2565/107—Alteration in the property of hybridised versus free label oligonucleotides
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    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/501—Detection characterised by immobilisation to a surface being an array of oligonucleotides

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN200880101316A 2007-05-31 2008-05-30 基于空间位阻和酶相关信号放大的高特异性和高灵敏度检测 Pending CN101849185A (zh)

Applications Claiming Priority (3)

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US94105707P 2007-05-31 2007-05-31
US60/941,057 2007-05-31
PCT/US2008/065286 WO2009017878A2 (en) 2007-05-31 2008-05-30 High specificity and high sensitivity detection based on steric hindrance & enzyme-related signal amplification

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US (2) US20100248231A1 (https=)
EP (1) EP2158482B1 (https=)
JP (1) JP5389789B2 (https=)
KR (1) KR20100038330A (https=)
CN (1) CN101849185A (https=)
CA (1) CA2688155C (https=)
WO (1) WO2009017878A2 (https=)

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CN115112883A (zh) * 2022-06-29 2022-09-27 深圳市德大科技有限公司 一种miR-Chr8:96检测试剂盒及其检测方法
CN116660345A (zh) * 2023-04-25 2023-08-29 苏州中科苏净生物技术有限公司 一种基于CRISPR/Cas的电化学核酸检测传感元件及检测方法
WO2024221502A1 (zh) * 2023-04-25 2024-10-31 苏州中科苏净生物技术有限公司 一种基于RPA与CRISPR/Cas的电化学核酸检测传感元件及检测方法

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US10083273B2 (en) 2005-07-29 2018-09-25 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
US11111544B2 (en) 2005-07-29 2021-09-07 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
US10081839B2 (en) 2005-07-29 2018-09-25 Natera, Inc System and method for cleaning noisy genetic data and determining chromosome copy number
CN102171565B (zh) 2008-08-04 2015-04-29 纳特拉公司 等位基因调用和倍性调用的方法
JP2011067174A (ja) * 2009-09-28 2011-04-07 Fujitsu Ltd 標的検出装置及びその製造方法
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US20190010543A1 (en) 2010-05-18 2019-01-10 Natera, Inc. Methods for simultaneous amplification of target loci
US11939634B2 (en) 2010-05-18 2024-03-26 Natera, Inc. Methods for simultaneous amplification of target loci
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US10316362B2 (en) 2010-05-18 2019-06-11 Natera, Inc. Methods for simultaneous amplification of target loci
US12545960B2 (en) 2010-05-18 2026-02-10 Natera, Inc. Methods for simultaneous amplification of target loci
US11322224B2 (en) 2010-05-18 2022-05-03 Natera, Inc. Methods for non-invasive prenatal ploidy calling
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US12221653B2 (en) 2010-05-18 2025-02-11 Natera, Inc. Methods for simultaneous amplification of target loci
US11332785B2 (en) 2010-05-18 2022-05-17 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US9677118B2 (en) 2014-04-21 2017-06-13 Natera, Inc. Methods for simultaneous amplification of target loci
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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115112883A (zh) * 2022-06-29 2022-09-27 深圳市德大科技有限公司 一种miR-Chr8:96检测试剂盒及其检测方法
CN115112883B (zh) * 2022-06-29 2025-05-09 深圳市德大科技有限公司 一种miR-Chr8:96检测试剂盒及其检测方法
CN116660345A (zh) * 2023-04-25 2023-08-29 苏州中科苏净生物技术有限公司 一种基于CRISPR/Cas的电化学核酸检测传感元件及检测方法
WO2024221502A1 (zh) * 2023-04-25 2024-10-31 苏州中科苏净生物技术有限公司 一种基于RPA与CRISPR/Cas的电化学核酸检测传感元件及检测方法

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CA2688155A1 (en) 2009-02-05
JP2010528618A (ja) 2010-08-26
US20100248231A1 (en) 2010-09-30
KR20100038330A (ko) 2010-04-14
EP2158482A2 (en) 2010-03-03
EP2158482A4 (en) 2010-07-14
JP5389789B2 (ja) 2014-01-15
CA2688155C (en) 2020-02-11
US20130115594A1 (en) 2013-05-09
WO2009017878A9 (en) 2009-03-26
WO2009017878A3 (en) 2009-05-14
EP2158482B1 (en) 2017-01-18

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Application publication date: 20100929