KR20100038330A - 입체구조적 방해 및 효소 관련 신호 증폭 기반의 고 특이성 및 고 민감도 검출 - Google Patents

입체구조적 방해 및 효소 관련 신호 증폭 기반의 고 특이성 및 고 민감도 검출 Download PDF

Info

Publication number
KR20100038330A
KR20100038330A KR1020097027652A KR20097027652A KR20100038330A KR 20100038330 A KR20100038330 A KR 20100038330A KR 1020097027652 A KR1020097027652 A KR 1020097027652A KR 20097027652 A KR20097027652 A KR 20097027652A KR 20100038330 A KR20100038330 A KR 20100038330A
Authority
KR
South Korea
Prior art keywords
probe
signal
nucleic acid
target
detection
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
KR1020097027652A
Other languages
English (en)
Korean (ko)
Inventor
팡 웨이
베른하르트 지. 짐머만
데이비드 티. 더블유. 웡
Original Assignee
더 리젠츠 오브 더 유니버시티 오브 캘리포니아
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=40305157&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=KR20100038330(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by 더 리젠츠 오브 더 유니버시티 오브 캘리포니아 filed Critical 더 리젠츠 오브 더 유니버시티 오브 캘리포니아
Publication of KR20100038330A publication Critical patent/KR20100038330A/ko
Ceased legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30—Oligonucleotides characterised by their secondary structure
    • C12Q2525/301—Hairpin oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/131—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a member of a cognate binding pair, i.e. extends to antibodies, haptens, avidin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/10—Detection mode being characterised by the assay principle
    • C12Q2565/102—Multiple non-interacting labels
    • C12Q2565/1025—Multiple non-interacting labels labels being on the same oligonucleotide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/10—Detection mode being characterised by the assay principle
    • C12Q2565/107—Alteration in the property of hybridised versus free label oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/501—Detection characterised by immobilisation to a surface being an array of oligonucleotides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
KR1020097027652A 2007-05-31 2008-05-30 입체구조적 방해 및 효소 관련 신호 증폭 기반의 고 특이성 및 고 민감도 검출 Ceased KR20100038330A (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US94105707P 2007-05-31 2007-05-31
US60/941,057 2007-05-31

Publications (1)

Publication Number Publication Date
KR20100038330A true KR20100038330A (ko) 2010-04-14

Family

ID=40305157

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020097027652A Ceased KR20100038330A (ko) 2007-05-31 2008-05-30 입체구조적 방해 및 효소 관련 신호 증폭 기반의 고 특이성 및 고 민감도 검출

Country Status (7)

Country Link
US (2) US20100248231A1 (https=)
EP (1) EP2158482B1 (https=)
JP (1) JP5389789B2 (https=)
KR (1) KR20100038330A (https=)
CN (1) CN101849185A (https=)
CA (1) CA2688155C (https=)
WO (1) WO2009017878A2 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BE1028157B1 (fr) * 2020-04-07 2022-04-12 Vaccim Dispositif et procédé pour déterminer, au départ d'un échantillon préalablement prélevé chez un individu, qu'au moins un anticorps cible a été ou est présent chez ledit individu

Families Citing this family (60)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11111543B2 (en) 2005-07-29 2021-09-07 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
US8532930B2 (en) 2005-11-26 2013-09-10 Natera, Inc. Method for determining the number of copies of a chromosome in the genome of a target individual using genetic data from genetically related individuals
US9424392B2 (en) 2005-11-26 2016-08-23 Natera, Inc. System and method for cleaning noisy genetic data from target individuals using genetic data from genetically related individuals
US10083273B2 (en) 2005-07-29 2018-09-25 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
US11111544B2 (en) 2005-07-29 2021-09-07 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
US10081839B2 (en) 2005-07-29 2018-09-25 Natera, Inc System and method for cleaning noisy genetic data and determining chromosome copy number
CN102171565B (zh) 2008-08-04 2015-04-29 纳特拉公司 等位基因调用和倍性调用的方法
JP2011067174A (ja) * 2009-09-28 2011-04-07 Fujitsu Ltd 標的検出装置及びその製造方法
US20120185176A1 (en) 2009-09-30 2012-07-19 Natera, Inc. Methods for Non-Invasive Prenatal Ploidy Calling
US12152275B2 (en) 2010-05-18 2024-11-26 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US11332793B2 (en) 2010-05-18 2022-05-17 Natera, Inc. Methods for simultaneous amplification of target loci
US11408031B2 (en) 2010-05-18 2022-08-09 Natera, Inc. Methods for non-invasive prenatal paternity testing
EP2572003A4 (en) 2010-05-18 2016-01-13 Natera Inc METHOD FOR NONINVASIVE PRANATAL PLOIDIE ASSIGNMENT
US20190010543A1 (en) 2010-05-18 2019-01-10 Natera, Inc. Methods for simultaneous amplification of target loci
US11939634B2 (en) 2010-05-18 2024-03-26 Natera, Inc. Methods for simultaneous amplification of target loci
US11326208B2 (en) 2010-05-18 2022-05-10 Natera, Inc. Methods for nested PCR amplification of cell-free DNA
US10316362B2 (en) 2010-05-18 2019-06-11 Natera, Inc. Methods for simultaneous amplification of target loci
US12545960B2 (en) 2010-05-18 2026-02-10 Natera, Inc. Methods for simultaneous amplification of target loci
US11322224B2 (en) 2010-05-18 2022-05-03 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US11339429B2 (en) 2010-05-18 2022-05-24 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US12221653B2 (en) 2010-05-18 2025-02-11 Natera, Inc. Methods for simultaneous amplification of target loci
US11332785B2 (en) 2010-05-18 2022-05-17 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US9677118B2 (en) 2014-04-21 2017-06-13 Natera, Inc. Methods for simultaneous amplification of target loci
WO2012088456A2 (en) 2010-12-22 2012-06-28 Natera, Inc. Methods for non-invasive prenatal paternity testing
AU2011358564B9 (en) 2011-02-09 2017-07-13 Natera, Inc Methods for non-invasive prenatal ploidy calling
EP2715351B1 (en) * 2011-05-24 2016-10-19 The Regents of The University of California Method for exosomal biomarker detection by electric field-induced release and measurement
US9335292B2 (en) 2011-10-13 2016-05-10 Auburn University Electrochemical proximity assay
EP2831587B1 (en) 2012-03-27 2018-05-16 Ventana Medical Systems, Inc. Signaling conjugates and methods of use
WO2013153911A1 (ja) * 2012-04-12 2013-10-17 国立大学法人東京大学 核酸の定量方法、検出プローブ、検出プローブセット、及び核酸の検出方法
US20140100126A1 (en) 2012-08-17 2014-04-10 Natera, Inc. Method for Non-Invasive Prenatal Testing Using Parental Mosaicism Data
US10262755B2 (en) 2014-04-21 2019-04-16 Natera, Inc. Detecting cancer mutations and aneuploidy in chromosomal segments
US9499870B2 (en) 2013-09-27 2016-11-22 Natera, Inc. Cell free DNA diagnostic testing standards
US10577655B2 (en) 2013-09-27 2020-03-03 Natera, Inc. Cell free DNA diagnostic testing standards
US12492429B2 (en) 2014-04-21 2025-12-09 Natera, Inc. Detecting mutations and ploidy in chromosomal segments
EP3957749A1 (en) 2014-04-21 2022-02-23 Natera, Inc. Detecting tumour specific mutations in biopsies with whole exome sequencing and in cell-free samples
US20180173845A1 (en) 2014-06-05 2018-06-21 Natera, Inc. Systems and Methods for Detection of Aneuploidy
WO2016183106A1 (en) 2015-05-11 2016-11-17 Natera, Inc. Methods and compositions for determining ploidy
ES2753274T5 (es) * 2015-08-21 2023-05-26 42 Life Sciences Gmbh & Co Kg Composición y procedimiento de hibridación
WO2017181202A2 (en) 2016-04-15 2017-10-19 Natera, Inc. Methods for lung cancer detection
US11485996B2 (en) 2016-10-04 2022-11-01 Natera, Inc. Methods for characterizing copy number variation using proximity-litigation sequencing
GB201618485D0 (en) 2016-11-02 2016-12-14 Ucl Business Plc Method of detecting tumour recurrence
US10011870B2 (en) 2016-12-07 2018-07-03 Natera, Inc. Compositions and methods for identifying nucleic acid molecules
US10894976B2 (en) 2017-02-21 2021-01-19 Natera, Inc. Compositions, methods, and kits for isolating nucleic acids
US10852274B2 (en) 2017-03-09 2020-12-01 Auburn University Differential circuit for background correction in electrochemical measurements
US11505799B2 (en) 2017-07-07 2022-11-22 Innamed, Inc. Aptamers for measuring lipoprotein levels
CA3074349A1 (en) * 2017-09-29 2019-04-04 Boehringer Ingelheim Vetmedica Gmbh Sensor apparatus and method for testing a sample
JP2021506342A (ja) 2017-12-14 2021-02-22 ティーエーアイ ダイアグノスティックス インコーポレイテッドＴａｉ Ｄｉａｇｎｏｓｔｉｃｓ，Ｉｎｃ． 移植のための移植片適合性の評価
CN108444992B (zh) * 2018-02-09 2020-11-13 河南工业大学 一种黄曲霉毒素定量检测试剂盒及其检测方法
US12398389B2 (en) 2018-02-15 2025-08-26 Natera, Inc. Methods for isolating nucleic acids with size selection
US10871485B2 (en) 2018-04-13 2020-12-22 Rarecyte, Inc. Kits for labeling of biomarkers and methods of using the same
CA3090426A1 (en) 2018-04-14 2019-10-17 Natera, Inc. Methods for cancer detection and monitoring by means of personalized detection of circulating tumor dna
US11560565B2 (en) 2018-06-13 2023-01-24 Auburn University Electrochemical detection nanostructure, systems, and uses thereof
US12234509B2 (en) 2018-07-03 2025-02-25 Natera, Inc. Methods for detection of donor-derived cell-free DNA
CN109406596B (zh) * 2018-12-13 2021-01-01 合肥工业大学 一种检测microRNA-21的电化学传感器、其制备方法及应用
EP3980559A1 (en) 2019-06-06 2022-04-13 Natera, Inc. Methods for detecting immune cell dna and monitoring immune system
CN113466445B (zh) * 2021-06-30 2023-06-30 陕西师范大学 基于杂交链式-酶显色反应检测Hg2+和Ag+的生物传感器及其制备方法和应用
CN115112883B (zh) * 2022-06-29 2025-05-09 深圳市德大科技有限公司 一种miR-Chr8:96检测试剂盒及其检测方法
JPWO2024157925A1 (https=) * 2023-01-26 2024-08-02
CN116626120B (zh) * 2023-04-25 2024-12-13 苏州中科苏净生物技术有限公司 一种基于RPA与CRISPR/Cas的电化学核酸检测传感元件及检测方法
CN116660345B (zh) * 2023-04-25 2024-09-06 苏州中科苏净生物技术有限公司 一种基于CRISPR/Cas的电化学核酸检测传感元件及检测方法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5925517A (en) * 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US7432048B2 (en) * 1996-11-29 2008-10-07 Third Wave Technologies, Inc. Reactions on a solid surface
US6287765B1 (en) * 1998-05-20 2001-09-11 Molecular Machines, Inc. Methods for detecting and identifying single molecules
US7118860B2 (en) * 1999-10-29 2006-10-10 Stratagene California Methods for detection of a target nucleic acid by capture
US7291457B2 (en) * 2001-06-21 2007-11-06 The Regents Of The University Of California Electrochemical detection of mismatch nucleic acids
WO2003000933A1 (en) * 2001-06-25 2003-01-03 Georgia Tech Research Corporation Dual resonance energy transfer nucleic acid probes
US20030096781A1 (en) * 2001-08-31 2003-05-22 University Of Southern California IL-8 is an autocrine growth factor and a surrogate marker for Kaposi's sarcoma
FR2834521B1 (fr) * 2002-01-10 2004-12-17 Bio Merieux Procede de detection et/ou d'identification de l'espece animale d'origine de la matiere animale contenue dans un echantillon
DE10220935B3 (de) * 2002-05-10 2004-02-05 Siemens Ag Verfahren für die biochemische Analytik von DNA und zugehörige Anordnung
WO2005001129A2 (en) * 2003-06-06 2005-01-06 Applera Corporation Mobility cassettes
WO2007033283A2 (en) * 2005-09-13 2007-03-22 University Of Hawaii Disposable electrode system for detection of selected nucleic acid sequences

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BE1028157B1 (fr) * 2020-04-07 2022-04-12 Vaccim Dispositif et procédé pour déterminer, au départ d'un échantillon préalablement prélevé chez un individu, qu'au moins un anticorps cible a été ou est présent chez ledit individu

Also Published As

Publication number Publication date
WO2009017878A2 (en) 2009-02-05
CA2688155A1 (en) 2009-02-05
JP2010528618A (ja) 2010-08-26
US20100248231A1 (en) 2010-09-30
EP2158482A2 (en) 2010-03-03
EP2158482A4 (en) 2010-07-14
JP5389789B2 (ja) 2014-01-15
CA2688155C (en) 2020-02-11
CN101849185A (zh) 2010-09-29
US20130115594A1 (en) 2013-05-09
WO2009017878A9 (en) 2009-03-26
WO2009017878A3 (en) 2009-05-14
EP2158482B1 (en) 2017-01-18

Similar Documents

Publication Publication Date Title
KR20100038330A (ko) 입체구조적 방해 및 효소 관련 신호 증폭 기반의 고 특이성 및 고 민감도 검출
Cheng et al. Recent advances in microRNA detection
Han et al. Facile and label-free electrochemical biosensors for microRNA detection based on DNA origami nanostructures
Zhang et al. Sensitive electrochemical biosensor for MicroRNAs based on duplex-specific nuclease-assisted target recycling followed with gold nanoparticles and enzymatic signal amplification
Labib et al. Electrochemical sensing of microRNAs: avenues and paradigms
Slinker et al. Multiplexed DNA-modified electrodes
L. Neo et al. G-quadruplex based probes for visual detection and sensing
Liang et al. Electrochemical biosensor for microRNA detection based on cascade hybridization chain reaction
JP4947355B2 (ja) 増加した感度を持つ核酸検出方法
Zhang et al. An isothermal electrochemical biosensor for the sensitive detection of microRNA based on a catalytic hairpin assembly and supersandwich amplification
CN103717752B (zh) 核酸分析法
US11156582B2 (en) Systems for detecting and quantifying nucleic acids
Shi et al. Cascaded strand displacement for non-enzymatic target recycling amplification and label-free electronic detection of microRNA from tumor cells
WO2022204427A1 (en) Crispr-mediated cleavage of oligonucleotide-detectable marker conjugates for detection of target analytes
He et al. Detection of biotin-streptavidin interactions based on poly (thymine)-templated copper nanoparticles coupled with Exo III-aided DNA recycling amplification
WO2008097190A1 (en) Methods of electrically detecting a nucleic acid by means of an electrode pair
Chen et al. Intercalation of quantum dots as the new signal acquisition and amplification platform for sensitive electrochemiluminescent detection of microRNA
Ho et al. Self-assembling protein platform for direct quantification of circulating microRNAs in serum with total internal reflection fluorescence microscopy
Wang et al. A Sandwich‐Type Electrochemical Biosensor for Detection of BCR/ABL Fusion Gene Using Locked Nucleic Acids on Gold Electrode
Hu et al. A signal-on electrochemical DNA biosensor based on potential-assisted Cu (I)-catalyzed azide-alkyne cycloaddition mediated labeling of hairpin-like oligonucleotide with electroactive probe
CN111051528A (zh) 传感器
Liu et al. An ultrasensitive electrochemical DNA sensing strategy free from pre-immobilization via G-quadruplex based homogenous proximity hybridization
Li et al. Sensitive fluorescent sensor for recognition of HIV‐1 dsDNA by using glucose oxidase and triplex DNA
WO2006132022A1 (ja) メチルシトシンの簡便検出法
US20040096859A1 (en) Method for detecting and/or quantifying an analyte

Legal Events

Date Code Title Description
PA0105 International application

Patent event date: 20091231

Patent event code: PA01051R01D

Comment text: International Patent Application

PG1501 Laying open of application
A201 Request for examination
PA0201 Request for examination

Patent event code: PA02012R01D

Patent event date: 20130530

Comment text: Request for Examination of Application

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

Comment text: Notification of reason for refusal

Patent event date: 20140807

Patent event code: PE09021S01D

E601 Decision to refuse application
PE0601 Decision on rejection of patent

Patent event date: 20141118

Comment text: Decision to Refuse Application

Patent event code: PE06012S01D

Patent event date: 20140807

Comment text: Notification of reason for refusal

Patent event code: PE06011S01I