JP2010524506A5 - - Google Patents

Download PDF

Info

Publication number
JP2010524506A5
JP2010524506A5 JP2010506503A JP2010506503A JP2010524506A5 JP 2010524506 A5 JP2010524506 A5 JP 2010524506A5 JP 2010506503 A JP2010506503 A JP 2010506503A JP 2010506503 A JP2010506503 A JP 2010506503A JP 2010524506 A5 JP2010524506 A5 JP 2010524506A5
Authority
JP
Japan
Prior art keywords
fluorescens
array
host cell
expression
cell population
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2010506503A
Other languages
English (en)
Japanese (ja)
Other versions
JP5444553B2 (ja
JP2010524506A (ja
Filing date
Publication date
Application filed filed Critical
Priority claimed from PCT/US2008/061483 external-priority patent/WO2008134461A2/en
Publication of JP2010524506A publication Critical patent/JP2010524506A/ja
Publication of JP2010524506A5 publication Critical patent/JP2010524506A5/ja
Application granted granted Critical
Publication of JP5444553B2 publication Critical patent/JP5444553B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

JP2010506503A 2007-04-27 2008-04-25 微生物宿主を迅速にスクリーニングして、異種タンパク質発現の収率および/または質が改善されている特定の株を同定する方法 Active JP5444553B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US91436107P 2007-04-27 2007-04-27
US60/914,361 2007-04-27
PCT/US2008/061483 WO2008134461A2 (en) 2007-04-27 2008-04-25 Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins

Publications (3)

Publication Number Publication Date
JP2010524506A JP2010524506A (ja) 2010-07-22
JP2010524506A5 true JP2010524506A5 (enExample) 2011-06-16
JP5444553B2 JP5444553B2 (ja) 2014-03-19

Family

ID=39739280

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2010506503A Active JP5444553B2 (ja) 2007-04-27 2008-04-25 微生物宿主を迅速にスクリーニングして、異種タンパク質発現の収率および/または質が改善されている特定の株を同定する方法

Country Status (10)

Country Link
US (1) US9394571B2 (enExample)
EP (2) EP2142651B1 (enExample)
JP (1) JP5444553B2 (enExample)
KR (1) KR101530395B1 (enExample)
CN (1) CN101688213A (enExample)
AU (1) AU2008245696B2 (enExample)
BR (1) BRPI0810120A2 (enExample)
CA (2) CA2964910C (enExample)
MX (1) MX2009011523A (enExample)
WO (1) WO2008134461A2 (enExample)

Families Citing this family (49)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9453251B2 (en) 2002-10-08 2016-09-27 Pfenex Inc. Expression of mammalian proteins in Pseudomonas fluorescens
AU2005269527B2 (en) 2004-07-26 2011-12-01 Pfenex Inc. Process for improved protein expression by strain engineering
CN102517280A (zh) * 2007-01-31 2012-06-27 菲尼克斯股份有限公司 用于提高表达的细菌前导序列
EP2142651B1 (en) 2007-04-27 2013-05-22 Pfenex Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
US9580719B2 (en) 2007-04-27 2017-02-28 Pfenex, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
WO2010008764A1 (en) * 2008-06-23 2010-01-21 Dow Global Technologies Inc. Pseudomonas fluorescens strains for production of extracellular recombinant protein
US9597379B1 (en) 2010-02-09 2017-03-21 David Gordon Bermudes Protease inhibitor combination with therapeutic proteins including antibodies
US8524220B1 (en) 2010-02-09 2013-09-03 David Gordon Bermudes Protease inhibitor: protease sensitivity expression system composition and methods improving the therapeutic activity and specificity of proteins delivered by bacteria
US8771669B1 (en) 2010-02-09 2014-07-08 David Gordon Bermudes Immunization and/or treatment of parasites and infectious agents by live bacteria
AU2011223627B2 (en) 2010-03-04 2015-06-18 Pfenex Inc. Method for producing soluble recombinant interferon protein without denaturing
AU2010201410B2 (en) * 2010-03-30 2015-04-30 Pelican Technology Holdings, Inc. High level expression of recombinant CRM197
MX343356B (es) 2010-03-30 2016-11-03 Pfenex Inc * Toxinas proteicas recombinantes de alto nivel de expresion.
NZ603033A (en) * 2010-04-01 2014-06-27 Pfenex Inc Methods for g-csf production in a pseudomonas host cell
US9169304B2 (en) 2012-05-01 2015-10-27 Pfenex Inc. Process for purifying recombinant Plasmodium falciparum circumsporozoite protein
US20150197738A1 (en) * 2012-07-09 2015-07-16 Alvine Pharmaceuticals, Inc. Acid Stable Prolyl Endopeptidases for Degrading Gluten
BR112015013557B1 (pt) 2012-12-11 2021-12-14 Albert Einstein College Of Medicine Sistema, método, método para determinar o efeito de um resíduo de aminoácido predeterminado de uma primeira proteína sobre a ligação da primeira proteína a uma segunda proteína e polipeptídeo pd-l1 mutante
US20140368723A1 (en) * 2013-06-18 2014-12-18 Samsung Electro-Mechanics Co., Ltd. Lens module and camera module including the same
US9737592B1 (en) 2014-02-14 2017-08-22 David Gordon Bermudes Topical and orally administered protease inhibitors and bacterial vectors for the treatment of disorders and methods of treatment
NZ732400A (en) * 2014-12-01 2021-07-30 Pfenex Inc Fusion partners for peptide production
US10676723B2 (en) 2015-05-11 2020-06-09 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
AU2017266905B2 (en) 2016-05-18 2022-12-15 Albert Einstein College Of Medicine, Inc. Variant PD-L1 polypeptides, T-cell modulatory multimeric polypeptides, and methods of use thereof
CA3019005A1 (en) 2016-05-18 2017-11-23 Cue Biopharma, Inc. T-cell modulatory multimeric polypeptides and methods of use thereof
HUE044665T2 (hu) 2016-09-01 2019-11-28 Metax Inst Fuer Diaetetik Gmbh Fenilalanin-mentes fehérje PKU (fenilketonuria) kezelésére
EP3532600A4 (en) * 2016-10-19 2020-09-16 General Automation LAB Technologies Inc. HIGH RESOLUTION SYSTEMS, KITS, DEVICES AND METHODS FOR SCREENING MICRO-ORGANISMS AND OTHER HIGH-PERFORMANCE MICROBIOLOGICAL APPLICATIONS
US11180535B1 (en) 2016-12-07 2021-11-23 David Gordon Bermudes Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
JP7250677B2 (ja) 2016-12-22 2023-04-03 キュー バイオファーマ, インコーポレイテッド T細胞調節多量体ポリペプチド及びその使用方法
US11851471B2 (en) 2017-01-09 2023-12-26 Cue Biopharma, Inc. T-cell modulatory multimeric polypeptides and methods of use thereof
KR102619015B1 (ko) 2017-03-15 2023-12-28 큐 바이오파마, 인크. 면역 반응을 조절하는 방법
CN110914912A (zh) * 2017-06-06 2020-03-24 齐默尔根公司 对基因修饰进行优先级排序以增加表型优化的吞吐量
CN110869502A (zh) * 2017-06-06 2020-03-06 齐默尔根公司 高通量转座子诱变
AU2018354067B2 (en) 2017-10-27 2023-03-30 Pfenex Inc. Method for production of recombinant Erwinia asparaginase
JP7358347B2 (ja) * 2017-10-27 2023-10-10 フェネックス インク. 組み換えE.coliアスパラギナーゼの製造のための方法
BR112020008369A2 (pt) 2017-10-27 2020-11-03 Pfenex Inc. sequências líder bacterianas para a expressão de proteína periplásmica
CN112041486A (zh) 2017-12-11 2020-12-04 鲍鱼生物股份有限公司 周质空间中蛋白质的酵母展示
CN108251330B (zh) * 2017-12-28 2021-03-23 江苏世邦生物工程科技有限公司 用于治理土壤污染的复合微生物菌剂及其制备方法和应用
EP3737689A4 (en) 2018-01-09 2021-12-01 Cue Biopharma, Inc. MULTIMER POLYPEPTIDES T-LYMPHOCYTE MODULATORS AND THEIR METHODS OF USE
AU2020206751A1 (en) 2019-01-07 2021-07-01 Pivot Bio, Inc. Plant colonization assays using natural microbial barcodes
CN109825464B (zh) * 2019-01-17 2021-06-15 浙江海洋大学 敲除t6ss-1基因簇的杀香鱼假单胞菌鱼用减毒疫苗
CN110041139B (zh) * 2019-05-30 2021-11-30 沈阳农业大学 一种用于连作土壤修复的生物菌多层带剂及使用方法
JP7218878B2 (ja) * 2019-08-07 2023-02-07 学校法人東京歯科大学 判別方法、蛍光測定装置および検査薬
US12285437B2 (en) 2019-10-30 2025-04-29 The Research Foundation For The State University Of New York Reversing the undesirable pH-profile of doxorubicin via activation of a disubstituted maleamic acid prodrug at tumor acidity
CN110898080B (zh) * 2019-11-01 2021-07-06 广西中医药大学 鲎血浆在促生长发育中的应用
CA3169949A1 (en) 2020-05-12 2021-11-18 Cue Biopharma, Inc. Multimeric t-cell modulatory polypeptides and methods of use thereof
JP2023541366A (ja) 2020-09-09 2023-10-02 キュー バイオファーマ, インコーポレイテッド 1型真性糖尿病(t1d)を治療するためのmhcクラスii t細胞調節多量体ポリペプチド及びその使用方法
US20220313798A1 (en) 2021-03-30 2022-10-06 Jazz Pharmaceuticals Ireland Ltd. Dosing of recombinant l-asparaginase
CN115725476B (zh) * 2022-12-21 2025-02-18 中国科学院沈阳应用生态研究所 一株边缘假单胞菌及其应用
CN115838712B (zh) * 2022-12-27 2024-05-14 华熙生物科技股份有限公司 具有肌肽水解酶功能的蛋白酶及其在l-肌肽合成中的应用
WO2024186259A1 (en) * 2023-03-07 2024-09-12 Karyolaimos Alexandros A method for enhancing recombinant production in a production host, an expression system, a library, a platform system, a production strain, an e. coli strain, a kit of parts, a method of using the platform system and a computer program, related to the method

Family Cites Families (305)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6696561B1 (en) 1909-07-09 2004-02-24 Basf Aktiengesellschaft Corynebacterium glutamicum genes encoding proteins involved in membrane synthesis and membrane transport
GB1381306A (en) 1972-03-03 1975-01-22 Ici Ltd Separating bacterial cells from a liquid medium
US3844893A (en) 1973-02-12 1974-10-29 Phillips Petroleum Co Microbial synthesis
US4169010A (en) 1976-11-18 1979-09-25 Phillips Petroleum Company Fermentation process for improved oxygen utilization
US4551433A (en) 1981-05-18 1985-11-05 Genentech, Inc. Microbial hybrid promoters
US5399684A (en) 1982-05-20 1995-03-21 Washington Research Foundation DNA sequences expressing mammalian alpha-1-antitrypsin
US4595658A (en) 1982-09-13 1986-06-17 The Rockefeller University Method for facilitating externalization of proteins synthesized in bacteria
US5151350A (en) 1982-10-27 1992-09-29 Repligen Corporation Cloned genes encoding recombinant protein a
US4432895A (en) 1982-11-24 1984-02-21 Hoffmann-La Roche Inc. Monomeric interferons
US4511503A (en) 1982-12-22 1985-04-16 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
GB8308483D0 (en) 1983-03-28 1983-05-05 Health Lab Service Board Secretion of gene products
US4755465A (en) 1983-04-25 1988-07-05 Genentech, Inc. Secretion of correctly processed human growth hormone in E. coli and Pseudomonas
US4680264A (en) 1983-07-01 1987-07-14 Lubrizol Genetics, Inc. Class II mobilizable gram-negative plasmid
US5281532A (en) 1983-07-27 1994-01-25 Mycogen Corporation Pseudomas hosts transformed with bacillus endotoxin genes
US4637980A (en) 1983-08-09 1987-01-20 Smithkline Beckman Corporation Externalization of products of bacteria
EP0155189A3 (en) 1984-03-16 1987-09-16 Genentech, Inc. Expression of cell wall degrading proteins and host cells harboring dna encoding such protein
SU1364343A1 (ru) 1984-07-13 1988-01-07 Всесоюзный научно-исследовательский институт генетики и селекции промышленных микроорганизмов Способ получени человеческого лейкоцитарного интерферона альфа-2
US4963495A (en) 1984-10-05 1990-10-16 Genentech, Inc. Secretion of heterologous proteins
ATE78515T1 (de) 1984-10-05 1992-08-15 Genentech Inc Dna, zellkulturen und verfahren zur sekretion von heterologen proteinen und periplasmische proteinrueckgewinnung.
US4695462A (en) 1985-06-28 1987-09-22 Mycogen Corporation Cellular encapsulation of biological pesticides
US4695455A (en) 1985-01-22 1987-09-22 Mycogen Corporation Cellular encapsulation of pesticides produced by expression of heterologous genes
GB8517071D0 (en) 1985-07-05 1985-08-14 Hoffmann La Roche Gram-positive expression control sequence
US4888274A (en) 1985-09-18 1989-12-19 Yale University RecA nucleoprotein filament and methods
US5618920A (en) 1985-11-01 1997-04-08 Xoma Corporation Modular assembly of antibody genes, antibodies prepared thereby and use
GB8529014D0 (en) 1985-11-25 1986-01-02 Biogen Nv Enhanced secretion of heterologous proteins
DE3751066T2 (de) 1986-03-24 1995-06-08 Getty Scient Dev Co Herstellungsverfahren von zuckernukleotiden mittels rekombinantverfahrens.
JP2612583B2 (ja) 1986-03-24 1997-05-21 ゲティ サイエンティフィック デベロップメント カンパニー キサンタンガムの組換dnaによる製造
US5559015A (en) 1986-03-26 1996-09-24 Getty Scientific Development Company Recombinant-DNA mediated production of xanthan gum
US5232840A (en) 1986-03-27 1993-08-03 Monsanto Company Enhanced protein production in bacteria by employing a novel ribosome binding site
US5292507A (en) 1986-08-01 1994-03-08 Imperial Oil Limited Method of using polysaccharides to stabilize microorganisms for inoculating plant seeds
US5165927A (en) 1986-08-01 1992-11-24 University Of Southern California Composition with modified pertussis toxin
US5541308A (en) 1986-11-24 1996-07-30 Gen-Probe Incorporated Nucleic acid probes for detection and/or quantitation of non-viral organisms
US5084559A (en) 1987-03-27 1992-01-28 Repligen Corporation Protein a domain mutants
ATE109828T1 (de) 1987-04-23 1994-08-15 Monsanto Co Sekretion eines dem insulin ähnlichen wachstumsfaktors in e. coli.
US5591603A (en) 1987-08-28 1997-01-07 Novo Nordisk A/S Process for preparing aprotinin and aprotinin analogs in yeast cells
KR0168039B1 (ko) 1987-09-04 1999-01-15 로버트 디. 웨스트 재조합 dna에서 유도한 보르데텔라 외독소 소단위체의 유사체 및 그를 포함하는 백신
GB8727489D0 (en) 1987-11-24 1987-12-23 Connaught Lab Detoxification of pertussis toxin
US5128130A (en) 1988-01-22 1992-07-07 Mycogen Corporation Hybrid Bacillus thuringiensis gene, plasmid and transformed Pseudomonas fluorescens
US5055294A (en) 1988-03-03 1991-10-08 Mycogen Corporation Chimeric bacillus thuringiensis crystal protein gene comprising hd-73 and berliner 1715 toxin genes, transformed and expressed in pseudomonas fluorescens
JPH01257491A (ja) 1988-04-08 1989-10-13 Tosoh Corp 不溶性融合異種蛋白質の処理方法
IL90190A0 (en) 1988-05-09 1989-12-15 Us Health Vector for secretion of proteins directly into periplasm or culture medium and method for synthesizing pure proteins utilizing the same
US5169772A (en) 1988-06-06 1992-12-08 Massachusetts Institute Of Technology Large scale method for purification of high purity heparinase from flavobacterium heparinum
JPH04500754A (ja) 1988-09-30 1992-02-13 アライド―シグナル・インコーポレーテッド 異種遺伝子発現のための改良細菌株
US6780613B1 (en) 1988-10-28 2004-08-24 Genentech, Inc. Growth hormone variants
US5552302A (en) 1989-04-24 1996-09-03 Promega Corporation Methods and compositions for production of human recombinant placental ribonuclease inhibitor
US6040138A (en) 1995-09-15 2000-03-21 Affymetrix, Inc. Expression monitoring by hybridization to high density oligonucleotide arrays
US6551784B2 (en) 1989-06-07 2003-04-22 Affymetrix Inc Method of comparing nucleic acid sequences
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5744101A (en) 1989-06-07 1998-04-28 Affymax Technologies N.V. Photolabile nucleoside protecting groups
DE3920358A1 (de) 1989-06-22 1991-01-17 Behringwerke Ag Bispezifische und oligospezifische, mono- und oligovalente antikoerperkonstrukte, ihre herstellung und verwendung
US5683888A (en) 1989-07-22 1997-11-04 University Of Wales College Of Medicine Modified bioluminescent proteins and their use
US5169760A (en) 1989-07-27 1992-12-08 Mycogen Corporation Method, vectors, and host cells for the control of expression of heterologous genes from lac operated promoters
US5023171A (en) 1989-08-10 1991-06-11 Mayo Foundation For Medical Education And Research Method for gene splicing by overlap extension using the polymerase chain reaction
EP0430645B1 (en) 1989-11-28 1993-08-18 The Wellcome Foundation Limited Vaccines
US5292658A (en) 1989-12-29 1994-03-08 University Of Georgia Research Foundation, Inc. Boyd Graduate Studies Research Center Cloning and expressions of Renilla luciferase
US5641671A (en) 1990-07-06 1997-06-24 Unilever Patent Holdings B.V. Production of active Pseudomonas glumae lipase in homologous or heterologous hosts
US5639949A (en) 1990-08-20 1997-06-17 Ciba-Geigy Corporation Genes for the synthesis of antipathogenic substances
US5670350A (en) 1990-08-20 1997-09-23 Novartis Finance Corporation Genomic DNA encoding a pseudomonas global transcriptional activation element and its use in activating gene expression
US5662898A (en) 1990-08-20 1997-09-02 Ciba-Geigy Corporation Genes for the synthesis of antipathogenic substances
US5610044A (en) 1990-10-01 1997-03-11 Lam; Stephen T. Microorganisms with mannopine catabolizing ability
JP2679390B2 (ja) 1990-10-12 1997-11-19 日本電気株式会社 コード設定回路
US5264365A (en) 1990-11-09 1993-11-23 Board Of Regents, The University Of Texas System Protease-deficient bacterial strains for production of proteolytically sensitive polypeptides
US5508192A (en) * 1990-11-09 1996-04-16 Board Of Regents, The University Of Texas System Bacterial host strains for producing proteolytically sensitive polypeptides
EP0834575B1 (en) 1990-12-06 2001-11-28 Affymetrix, Inc. (a Delaware Corporation) Identification of nucleic acids in samples
ES2142801T3 (es) 1991-03-11 2000-05-01 Univ Georgia Res Found Clonacion y expresion de luciferasa de renilla.
US6800738B1 (en) 1991-06-14 2004-10-05 Genentech, Inc. Method for making humanized antibodies
US5558862A (en) 1991-09-23 1996-09-24 Monsanto Company Method of controlling insects
US5348867A (en) 1991-11-15 1994-09-20 George Georgiou Expression of proteins on bacterial surface
EP0617706B1 (en) 1991-11-25 2001-10-17 Enzon, Inc. Multivalent antigen-binding proteins
IL104419A0 (en) 1992-01-24 1993-05-13 Fmc Corp Insecticidally effective peptides
GB9206645D0 (en) 1992-03-26 1992-05-06 Mini Agriculture & Fisheries Biological control of post harvest pests
DK154292D0 (da) 1992-12-23 1992-12-23 Novo Nordisk As Nyt enzym
US5652128A (en) 1993-01-05 1997-07-29 Jarvik; Jonathan Wallace Method for producing tagged genes, transcripts, and proteins
US5581476A (en) 1993-01-28 1996-12-03 Amgen Inc. Computer-based methods and articles of manufacture for preparing G-CSF analogs
GB9314886D0 (en) 1993-07-19 1993-09-01 Zeneca Ltd Production of a biological control agent
US5914254A (en) 1993-08-02 1999-06-22 Celtrix Pharmaceuticals, Inc. Expression of fusion polypeptides transported out of the cytoplasm without leader sequences
DE69435112D1 (de) 1993-09-10 2008-08-21 Univ Columbia Verwendung von grünem fluoreszenzprotein
TW300879B (enExample) 1993-11-10 1997-03-21 Ibm
JPH09506508A (ja) 1993-12-03 1997-06-30 メディカル リサーチ カウンシル 組換え結合タンパク質およびペプチド
WO1995018634A1 (en) 1994-01-04 1995-07-13 The Scripps Research Institute Human monoclonal antibodies to herpes simplex virus and methods therefor
WO1995021191A1 (en) 1994-02-04 1995-08-10 William Ward Bioluminescent indicator based upon the expression of a gene for a modified green-fluorescent protein
US7294331B2 (en) 1994-03-07 2007-11-13 Immunex Corporation Methods of using flt3-ligand in hematopoietic cell transplantation
AUPM516994A0 (en) 1994-04-20 1994-05-12 Gene Shears Pty. Limited An in vivo gene expression system
US5527883A (en) 1994-05-06 1996-06-18 Mycogen Corporation Delta-endotoxin expression in pseudomonas fluorescens
US6117670A (en) 1994-06-08 2000-09-12 Novartis Finance Corporation Pyrrolnitrin biosynthesis genes and uses thereof
CA2150526C (en) 1994-06-09 2005-11-15 Andreas Kiener Biotechnological process for the preparation of cyclic s-.alpha.-amino carboxylic acids and r-.alpha.-amino carboxamides
US5989808A (en) 1994-06-14 1999-11-23 American Cyanamid Company Identification of compounds affecting specific interaction of peptide binding pairs
EP0787200B1 (en) 1994-10-28 2005-04-20 The Trustees Of The University Of Pennsylvania Improved adenovirus and methods of use thereof
US5777079A (en) 1994-11-10 1998-07-07 The Regents Of The University Of California Modified green fluorescent proteins
US5625048A (en) 1994-11-10 1997-04-29 The Regents Of The University Of California Modified green fluorescent proteins
EP0797671B1 (en) 1994-12-09 2004-03-03 Novo Nordisk A/S A process of producing extracellular proteins in bacteria
US5958713A (en) 1995-01-31 1999-09-28 Novo Nordisk A/S Method of detecting biologically active substances by using green fluorescent protein
US5641870A (en) 1995-04-20 1997-06-24 Genentech, Inc. Low pH hydrophobic interaction chromatography for antibody purification
ZA963530B (en) 1995-05-05 1996-11-05 Hoffmann La Roche Recombinant obese (ob) proteins
US5711945A (en) 1995-05-11 1998-01-27 Regents Of The University Of Minnesota Pitch degradation with Pseudomonas fluorescens
FR2734842B1 (fr) 1995-06-02 1998-02-27 Rhone Poulenc Agrochimie Sequence adn d'un gene de l'hydroxy-phenyl pyruvate dioxygenase et obtention de plantes contenant un gene de l'hydroxy-phenyl pyruvate dioxygenase, tolerantes a certains herbicides
US6083690A (en) 1995-06-02 2000-07-04 Osteoscreen, Inc. Methods and compositions for identifying osteogenic agents
US5955298A (en) 1995-06-26 1999-09-21 The United States Of America As Represented By The Secretary Of Agriculture Sequences for production of 2,4-Diacetylphloroglucinol and methods
US6495357B1 (en) 1995-07-14 2002-12-17 Novozyme A/S Lipolytic enzymes
US6020473A (en) 1995-08-25 2000-02-01 Genentech, Inc. Nucleic acids encoding variants of vascular endothelial cell growth factor
CA2232727C (en) 1995-09-22 2002-03-26 Novo Nordisk A/S Novel variants of green fluorescent protein, gfp
US5952236A (en) 1995-10-26 1999-09-14 Thompson; Richard B. Enzyme-based fluorescence biosensor for chemical analysis
US5968738A (en) 1995-12-06 1999-10-19 The Board Of Trustees Of The Leland Stanford Junior University Two-reporter FACS analysis of mammalian cells using green fluorescent proteins
US5776730A (en) 1995-12-15 1998-07-07 University Of Hawaii Neurospora hosts for the production of recombinant proteins, and methods for producing same
US6020192A (en) 1996-01-18 2000-02-01 University Of Florida Humanized green fluorescent protein genes and methods
US5874304A (en) 1996-01-18 1999-02-23 University Of Florida Research Foundation, Inc. Humanized green fluorescent protein genes and methods
EP0880598A4 (en) 1996-01-23 2005-02-23 Affymetrix Inc RAPID EVALUATION OF NUCLEIC ACID ABUNDANCE DIFFERENCE, WITH A HIGH-DENSITY OLIGONUCLEOTIDE SYSTEM
US5804387A (en) 1996-02-01 1998-09-08 The Board Of Trustees Of The Leland Stanford Junior University FACS-optimized mutants of the green fluorescent protein (GFP)
US5876995A (en) 1996-02-06 1999-03-02 Bryan; Bruce Bioluminescent novelty items
US6255558B1 (en) 1996-02-14 2001-07-03 Btg International Limited Gene expression
US6121247A (en) 1996-03-29 2000-09-19 The Johns Hopkins University Therapy for allergic diseases
US5741663A (en) 1996-04-16 1998-04-21 University Of Georgia Research Foundation, Inc. Selective additive and media for Pseudomonas fluorescens
US6218185B1 (en) 1996-04-19 2001-04-17 The United States Of America As Represented By The Secretary Of Agriculture Piggybac transposon-based genetic transformation system for insects
US6699838B1 (en) 1996-05-03 2004-03-02 Abbott Laboratories Antiangiogenic peptides
US6096865A (en) 1996-05-06 2000-08-01 Amgen Inc. Mutants of the green fluorescent protein having improved fluorescent properties at 37°
US6027881A (en) 1996-05-08 2000-02-22 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Mutant Aequorea victoria fluorescent proteins having increased cellular fluorescence
US5948889A (en) 1996-05-21 1999-09-07 Case Western Reserve University Compositions and methods for screening antimicrobials
US5912137A (en) 1996-07-16 1999-06-15 The Regents Of The University Of California Assays for protein kinases using fluorescent
US5925558A (en) 1996-07-16 1999-07-20 The Regents Of The University Of California Assays for protein kinases using fluorescent protein substrates
US7129061B1 (en) * 1996-08-07 2006-10-31 Biogen Idec Ma Inc. Tumor necrosis factor related ligand
US5948681A (en) 1996-08-14 1999-09-07 Children's Hospital Of Philadelphia Non-viral vehicles for use in gene transfer
US6124128A (en) 1996-08-16 2000-09-26 The Regents Of The University Of California Long wavelength engineered fluorescent proteins
US5914233A (en) 1996-08-23 1999-06-22 Osteo Screen Screening assay for the identification of agents which alter expression of PTH-rP
WO1998008839A1 (en) 1996-08-26 1998-03-05 Eli Lilly And Company Combinatorial process for preparing substituted thiophene libraries
CA2264499A1 (en) 1996-09-06 1998-03-12 The Trustees Of The University Of Pennsylvania Methods using cre-lox for production of recombinant adeno-associated viruses
US6255071B1 (en) 1996-09-20 2001-07-03 Cold Spring Harbor Laboratory Mammalian viral vectors and their uses
US6025192A (en) 1996-09-20 2000-02-15 Cold Spring Harbor Laboratory Modified retroviral vectors
US5976796A (en) 1996-10-04 1999-11-02 Loma Linda University Construction and expression of renilla luciferase and green fluorescent protein fusion genes
US6136566A (en) 1996-10-04 2000-10-24 Lexicon Graphics Incorporated Indexed library of cells containing genomic modifications and methods of making and utilizing the same
TW371617B (en) 1996-10-09 1999-10-11 Of Animal And Plant Health Inspection And Quarantine Council Of Agriculture Executive Yuan Bureau Method to transplant GFP into autographa californica multiple nuclear polyhedrosis virus for inflicting pest in an attempt to detect and flow up it existence and to improve life span against UV
US5757051A (en) 1996-11-12 1998-05-26 Micron Technology, Inc. Static memory cell and method of manufacturing a static memory cell
US6060247A (en) 1996-11-18 2000-05-09 Mcgill University Post-mitotic neurons containing adenovirus vectors that modulate apoptosis and growth
US5955348A (en) 1997-11-25 1999-09-21 Novartis Ag Genetically modified pseudomonas strains with enhanced biocontrol activity
AU5854498A (en) 1996-12-06 1998-06-29 Novartis Ag Genetically modified (pseudomonas) strains with enhanced biocontrol activity
US5891688A (en) 1996-12-06 1999-04-06 Novartis Finance Corporation DNA encoding lemA-independent GacA and its use in activating gene expression
US5756087A (en) 1996-12-06 1998-05-26 Novartis Finance Corporation Genetically modified Pseudomonas strains with enhanced biocontrol activity
JP3795533B2 (ja) 1996-12-12 2006-07-12 プロルーム・リミテツド 感染性物質を検出及び同定するための方法及び装置
US6194194B1 (en) 1996-12-23 2001-02-27 Daniel Molloy Method for controlling dreissena species
DE951551T1 (de) 1996-12-23 2000-09-14 Immunex Corp., Seattle Ligand für rezeptor aktivator of nf-kappa b, ligand ist mitglied der tnf superfamilie
AU736267B2 (en) 1996-12-31 2001-07-26 Omeros Corporation Vectors and methods for the mutagenesis of mammalian genes
US6090919A (en) 1997-01-31 2000-07-18 The Board Of Trustees Of The Leland Stanford Junior University FACS-optimized mutants of the green fluorescent protein (GFP)
US5994077A (en) 1997-01-31 1999-11-30 The Board Of Trustees Of The Leland Stanford Junior University Flourescence-based isolation of differentially induced genes
US5795759A (en) 1997-03-10 1998-08-18 The University Of Iowa Research Foundation Carboxylic acid reductase, and methods of using same
US6197928B1 (en) 1997-03-14 2001-03-06 The Regents Of The University Of California Fluorescent protein sensors for detection of analytes
US5994071A (en) 1997-04-04 1999-11-30 Albany Medical College Assessment of prostate cancer
US5952208A (en) 1997-04-07 1999-09-14 Energy Biosystems Corporation Dsz gene expression in pseudomonas hosts
US6251384B1 (en) 1997-04-28 2001-06-26 Anticancer, Inc. Metastasis models using green fluorescent protein (GFP) as a marker
US5993778A (en) 1997-05-07 1999-11-30 Firestein; Stuart J. Functional expression of, and assay for, functional cellular receptors in vivo
US6225082B1 (en) 1997-05-09 2001-05-01 Research Corporation Technologies, Inc. Myelin basic protein MRNA transport and translation enhancer sequences
US6303373B1 (en) 1999-06-09 2001-10-16 Whitehead Institute For Biomedical Research Method of measuring plasma membrane targeting of GLUT4
US6251582B1 (en) 1997-07-17 2001-06-26 New York University Alternative G-coupled receptors associated with retroviral entry into cells, methods of identifying the same, and diagnostic and therapeutic uses thereof
US6229911B1 (en) 1997-07-25 2001-05-08 Affymetrix, Inc. Method and apparatus for providing a bioinformatics database
US6420108B2 (en) 1998-02-09 2002-07-16 Affymetrix, Inc. Computer-aided display for comparative gene expression
IL121404A0 (en) 1997-07-27 1998-01-04 Yissum Res Dev Co Transgenic higher plants of altered structural morphology
US6221612B1 (en) 1997-08-01 2001-04-24 Aurora Biosciences Corporation Photon reducing agents for use in fluorescence assays
US6214563B1 (en) 1997-08-01 2001-04-10 Aurora Biosciences Corporation Photon reducing agents for reducing undesired light emission in assays
US6251677B1 (en) 1997-08-25 2001-06-26 The Trustees Of The University Of Pennsylvania Hybrid adenovirus-AAV virus and methods of use thereof
HUP9701446A1 (hu) 1997-08-27 1999-05-28 Phylaxia-Pharma Gyógyszer-, Oltóanyag és Agrobiológiai Készítményeket Gyártó és Forgalmazó Rt. Eljárás a talaj mikroorganizmus populációjának előnyös kialakítására
US6153409A (en) 1997-09-11 2000-11-28 University Of Maryland Biotechnology Institute Process for continuous optimized protein production in insect larvae
US6071738A (en) 1997-09-19 2000-06-06 Energy Biosystems Corporation Conversion of organosulfur compounds to oxyorganosulfur compounds for desulfurization of fossil fuels
DK1017803T3 (da) 1997-09-26 2010-08-23 Abt Holding Co Ekspression af endogene gener ved ikke-homolog rekombination af et vektorkonstrukt med cellulært DNA
US6130313A (en) 1997-10-02 2000-10-10 Clontech Laboratories, Inc. Rapidly degrading GFP-fusion proteins
US6133429A (en) 1997-10-03 2000-10-17 Becton Dickinson And Company Chromophores useful for the preparation of novel tandem conjugates
US6284496B1 (en) 1997-10-03 2001-09-04 University Of South Florida DNA vector for determining the presence of out-of-reading-frame mutations
US6361989B1 (en) 1997-10-13 2002-03-26 Novozymes A/S α-amylase and α-amylase variants
US5968773A (en) 1997-11-14 1999-10-19 Heddle; John A. System and method for regulation of gene expression
US6447770B1 (en) 1997-11-20 2002-09-10 The United States Of America As Represented By The Secretary Of Agriculture Biocontrol agents for take-all
US6277625B1 (en) 1997-11-20 2001-08-21 The United States Of America As Represented By The Secretary Of Agriculture Transgenic strains of Pseudomonas for biocontrol of plant root diseases
US6013447A (en) 1997-11-21 2000-01-11 Innovir Laboratories, Inc. Random intracellular method for obtaining optimally active nucleic acid molecules
AU755032B2 (en) 1997-12-12 2002-11-28 Regents Of The University Of California, The Methods for defining cell types
US6060594A (en) 1997-12-18 2000-05-09 Ecogen, Inc. Nucleic acid segments encoding modified bacillus thuringiensis coleopteran-toxic crystal proteins
US6506559B1 (en) 1997-12-23 2003-01-14 Carnegie Institute Of Washington Genetic inhibition by double-stranded RNA
US6136539A (en) 1998-02-11 2000-10-24 The Regents Of The University Of California Compositions and methods for the inhibition of MUC-5 mucin gene expression
US6156552A (en) 1998-02-18 2000-12-05 Novo Nordisk A/S Lipase variants
US5922576A (en) 1998-02-27 1999-07-13 The John Hopkins University Simplified system for generating recombinant adenoviruses
US6210910B1 (en) 1998-03-02 2001-04-03 Trustees Of Tufts College Optical fiber biosensor array comprising cell populations confined to microcavities
WO1999046592A1 (en) 1998-03-09 1999-09-16 The Regents Of The University Of California Regulation of the cell cycle by sterols
AUPP249298A0 (en) 1998-03-20 1998-04-23 Ag-Gene Australia Limited Synthetic genes and genetic constructs comprising same I
WO1999049019A2 (en) 1998-03-27 1999-09-30 Prolume, Ltd. Luciferases, fluorescent proteins, nucleic acids encoding the luciferases and fluorescent proteins and the use thereof in diagnostics
US6080576A (en) 1998-03-27 2000-06-27 Lexicon Genetics Incorporated Vectors for gene trapping and gene activation
US6248558B1 (en) 1998-03-31 2001-06-19 Vanderbilt University Sequence and method for genetic engineering of proteins with cell membrane translocating activity
AU760575C (en) 1998-04-13 2005-04-14 University Of Georgia Research Foundation, Inc., The Pyruvate carboxylase overexpression for enhanced production of oxaloacetate-derived biochemicals in microbial cells
US6037133A (en) 1998-04-17 2000-03-14 Clontech Laboratories, Inc. IκBEGFP constructs, cell lines and methods of use
JP4169478B2 (ja) 1998-04-21 2008-10-22 マイクロメット アーゲー Cd19×cd3特異的ポリペプチドおよびその使用
US6280934B1 (en) 1998-05-13 2001-08-28 Millennium Pharmaceuticals, Inc. Assay for agents which alter G-protein coupled receptor activity
US20030064435A1 (en) 1998-05-28 2003-04-03 Weiner Joel Hirsch Compositions and methods for protein secretion
US6140132A (en) 1998-06-09 2000-10-31 The Regents Of The University Of California Fluorescent protein sensors for measuring the pH of a biological sample
US6150176A (en) 1998-06-09 2000-11-21 The Regents Of The University Of California Fluorescent protein sensors for measuring the pH of a biological sample
WO1999066072A2 (en) 1998-06-16 1999-12-23 Nova Molecular, Inc. Methods for treating a neurological disease by determining bche genotype
US5989837A (en) 1998-07-13 1999-11-23 Wisconsin Alumni Research Foundation Immortalized human keratinocyte cell line
AUPP589798A0 (en) 1998-09-14 1998-10-08 Charles Sturt University Novel bacterial strains and methods of controlling fungal pathogens
US6185561B1 (en) 1998-09-17 2001-02-06 Affymetrix, Inc. Method and apparatus for providing and expression data mining database
US6323007B1 (en) 1998-09-18 2001-11-27 Novozymes A/S 2,6-β-D-fructan hydrolase enzyme and processes for using the enzyme
US6180343B1 (en) 1998-10-08 2001-01-30 Rigel Pharmaceuticals, Inc. Green fluorescent protein fusions with random peptides
US5985577A (en) 1998-10-14 1999-11-16 The Trustees Of Columbia University In The City Of New York Protein conjugates containing multimers of green fluorescent protein
US6203986B1 (en) 1998-10-22 2001-03-20 Robert H. Singer Visualization of RNA in living cells
US6268201B1 (en) 1998-10-23 2001-07-31 Albert Einstein College Of Medicine Of Yeshiva University IniB, iniA and iniC genes of mycobacteria and methods of use
CA2346797C (en) 1998-10-28 2008-09-23 Genentech, Inc. Process for recovering heterologous polypeptides from bacterial cells
US6270958B1 (en) 1998-10-29 2001-08-07 Washington University Detection of negative-strand RNA viruses
AU1722800A (en) 1998-11-18 2000-06-05 University Of Akron, The Production of biological materials by simultaneous aerobic and anaerobic respiration
US6210922B1 (en) 1998-11-30 2001-04-03 National Research Council Of Canada Serum free production of recombinant proteins and adenoviral vectors
US6136538A (en) 1999-01-08 2000-10-24 Washington University Silent inducible virus replicons and uses thereof
US6015557A (en) 1999-02-24 2000-01-18 Tobinick; Edward L. Tumor necrosis factor antagonists for the treatment of neurological disorders
KR100312456B1 (ko) 1999-03-13 2001-11-03 윤덕용 슈도모나스 플루오레슨스 유래의 외래단백질 분비촉진유전자
JP4732591B2 (ja) 1999-03-29 2011-07-27 ノボザイムス アクティーゼルスカブ 分枝酵素活性を有するポリペプチド及びそれをコードする核酸
US6960652B2 (en) * 1999-03-30 2005-11-01 Board Of Regents, The University Of Texas System Compositions and methods for modifying toxic effects of proteinaceous compounds
US6664386B1 (en) 1999-04-08 2003-12-16 The Research Foundation Of State University Of New York System for efficient secretion of recombinant proteins
CA2381284A1 (en) 1999-08-04 2001-02-15 Amgen Inc. Fhm, a novel member of the tnf ligand supergene family
US6558939B1 (en) 1999-08-31 2003-05-06 Novozymes, A/S Proteases and variants thereof
US7459540B1 (en) 1999-09-07 2008-12-02 Amgen Inc. Fibroblast growth factor-like polypeptides
DE19944870A1 (de) 1999-09-18 2001-03-29 Aventis Pharma Gmbh Signalsequenzen zur Herstellung von Leu-Hirudin über Sekretion durch E. coli in das Kulturmedium
WO2001027258A2 (en) 1999-10-13 2001-04-19 The University Of Georgia Research Foundation, Inc. High yield protein expression system and methods
US6607885B1 (en) 1999-10-15 2003-08-19 E. I. Du Pont De Nemours And Company Method for high-density microarray medicated gene expression profiling
US6617143B1 (en) 1999-10-20 2003-09-09 Novozymes A/S Polypeptides having glucanotransferase activity and nucleic acids encoding same
CA2388982A1 (en) * 1999-11-05 2001-05-10 Novozymes A/S Microtiter plate (mtp) based high throughput screening (hts) assays
HUP0203409A3 (en) 1999-11-12 2005-06-28 Maxygen Holdings Ltd Redwood C Interferon gamma conjugates
JP4981229B2 (ja) 2000-02-25 2012-07-18 ルードヴィッヒ インスティテュート フォー キャンサー リサーチ ハイブリッド血管内皮成長因子DNAsおよびタンパク質に関与する物質および方法
KR20080023768A (ko) 2000-03-30 2008-03-14 화이트헤드 인스티튜트 포 바이오메디칼 리서치 Rna 간섭의 rna 서열 특이적인 매개체
US6509181B1 (en) 2000-04-14 2003-01-21 Novozymes, A/S Polypeptides having haloperoxide activity
JP2001299360A (ja) 2000-04-28 2001-10-30 Morihiro Matsuda ヒト型抗破傷風毒素一本鎖組換え抗体フラグメント
CN1137999C (zh) 2000-07-04 2004-02-11 清华大学 集成式微阵列装置
GB0016702D0 (en) 2000-07-08 2000-08-23 Secr Defence Brit Expression system
US20030108980A1 (en) 2000-08-14 2003-06-12 Sayler Gary S. Bioluminescent methods for direct visual detection of environmental compounds
AU2001288360A1 (en) 2000-08-23 2002-03-04 Genome Therapeutics Corporation Genomics-assisted rapid identification of targets
US6673580B2 (en) 2000-10-27 2004-01-06 Genentech, Inc. Identification and modification of immunodominant epitopes in polypeptides
JP2002201141A (ja) 2000-10-27 2002-07-16 Hayashibara Biochem Lab Inc 蛋白質合成抑制遺伝子の発現増強剤
US7087412B2 (en) 2000-11-14 2006-08-08 Boehringer Ingelheim International Gmbh Methods for large scale protein production in prokaryotes
GB0027782D0 (en) 2000-11-14 2000-12-27 Boehringer Ingelheim Int Methods fro large scale protein productio in prokaryotes
EP1356052B1 (en) 2000-12-14 2008-08-20 Genentech, Inc. Production of full antibodies in procaryotic cells
US6979556B2 (en) 2000-12-14 2005-12-27 Genentech, Inc. Separate-cistron contructs for secretion of aglycosylated antibodies from prokaryotes
CZ304484B6 (cs) 2000-12-14 2014-05-21 Genentech, Inc. Bakteriální hostitelské kmeny a způsob tvorby polypeptidu
ATE446772T1 (de) 2001-01-05 2009-11-15 Viromed Ltd Zusammensetzungen zur gentherapie rheumatoider arthritis, die ein gen, das für ein anti- angiogenes protein oder teile davon kodiert, enthalten
US7202059B2 (en) 2001-02-20 2007-04-10 Sanofi-Aventis Deutschland Gmbh Fusion proteins capable of being secreted into a fermentation medium
DE10108212A1 (de) 2001-02-20 2002-08-22 Aventis Pharma Gmbh Fusionsprotein zur Sekretion von Wertprotein in bakterielle Überstände
US6887462B2 (en) 2001-04-09 2005-05-03 Chiron Corporation HSA-free formulations of interferon-beta
US7217785B2 (en) 2001-05-09 2007-05-15 The Regents Of The University Of California Cysteine-containing peptides having antioxidant properties
WO2002092000A2 (en) 2001-05-11 2002-11-21 Genome Therapeutics Corporation Hbm variants that modulate bone mass and lipid levels
CA2347657A1 (en) 2001-05-22 2002-11-22 University Of British Columbia Expression of heterologous peptides from caulobacter
ES2606290T3 (es) 2001-07-12 2017-03-23 University Of Massachusetts Producción in vivo de ARN de interferencia pequeños que median el silenciamiento génico
AU2002329667A1 (en) 2001-07-30 2003-02-17 Uta Griesenbach Specific inhibition of gene expression by small double stranded rnas
ATE439041T1 (de) 2001-09-13 2009-08-15 California Inst Of Techn Verfahren zur expression von kleinen antiviralen rna-molekülen innerhalb einer zelle
US7419783B2 (en) 2001-11-05 2008-09-02 Research Development Foundation Engineering of leader peptides for the secretion of recombinant proteins in bacteria
US20030114409A1 (en) 2001-11-16 2003-06-19 Mello Craig C. Facilitation of RNA interference
WO2003056022A2 (en) 2001-12-21 2003-07-10 Oxford Biomedica (Uk) Limited Method for producing a transgenic organism using a lentiviral expression vector such as eiav
US6806328B2 (en) 2002-01-28 2004-10-19 Univation Technologies, Llc Polymerization catalyst activator complexes and their use in a polymerization process
WO2003064621A2 (en) 2002-02-01 2003-08-07 Ambion, Inc. HIGH POTENCY siRNAS FOR REDUCING THE EXPRESSION OF TARGET GENES
AU2002306484A1 (en) * 2002-02-13 2003-09-04 Dow Global Technologies Inc. Over-expression of extremozyme genes in pseudomonads and closely related bacteria
EP1474433A4 (en) 2002-02-20 2005-02-23 Sirna Therapeutics Inc VALIDATION AND IDENTIFICATION OF RNA INTERFERENCE FACILITATED TARGETS USING SHORT INTERFERENT NUCLEIC ACID (siNA)
AU2003218217A1 (en) 2002-03-15 2003-09-29 The Trustees Of The University Of Pennsylvania Heterologous protein production using the twin arginine translocation pathway
JP4472998B2 (ja) 2002-04-22 2010-06-02 ダウ グローバル テクノロジーズ インコーポレイティド 低コストのペプチド製造
NZ571508A (en) 2002-05-24 2010-05-28 Schering Corp Neutralizing human anti-IGFR antibody
EP1535065A4 (en) 2002-06-11 2007-07-11 Burnham Inst NEUROPROTECTOR SYNERGY OF ERYTHROPOIETIN AND INSULIN-LIKE GROWTH FACTOR
AU2003299527A1 (en) 2002-06-17 2004-06-07 Jimmy D. Ballard Mutant of clostridium difficile toxin b and methods of use
CA2491178A1 (en) 2002-07-03 2004-01-15 Maxygen Holdings Ltd. Full-length interferon gamma polypeptide variants
AU2003256365B2 (en) 2002-07-03 2010-02-18 Pfenex Inc. Benzoate- and anthranilate-inducible promoters
AU2003243831B2 (en) 2002-07-11 2008-07-24 Sugar Research Australia Limited Transgenic plants used as a bioreactor system
NO20023581D0 (no) 2002-07-26 2002-07-26 Fmc Biopolymer As Nye mutantstammer av Pseudomonas fluorescens og varianter derav, metoder for produksjon og bruk derav til produksjon avalginat
US20040157289A1 (en) 2002-09-06 2004-08-12 Salerno John C. Protein expression system
US9453251B2 (en) * 2002-10-08 2016-09-27 Pfenex Inc. Expression of mammalian proteins in Pseudomonas fluorescens
PL376343A1 (en) 2002-10-08 2005-12-27 Dow Agrosciences Llc Amended recombinant cells (arcs) for the production and delivery of antiviral agents, adjuvants and vaccine accelerants
ATE517922T1 (de) * 2002-11-08 2011-08-15 Ablynx Nv Stabilisierte einzel-domänen-antikörper in einer pharmazeutischen zusammensetzung ausgestaltet für inhalation
AU2003286002B2 (en) 2002-11-08 2011-06-16 Ablynx N.V. Single domain antibodies directed against tumour necrosis factor-alpha and uses therefor
US20060034845A1 (en) 2002-11-08 2006-02-16 Karen Silence Single domain antibodies directed against tumor necrosis factor alpha and uses therefor
DE10258885A1 (de) 2002-12-17 2004-07-15 Aventis Pharma Deutschland Gmbh Verfahren zur Generierung eines gentechnisch veränderten Organismus
EP2390270A1 (en) * 2003-01-10 2011-11-30 Ablynx N.V. Therapeutic polypeptides, homologues thereof, fragments thereof and for use in modulating platelet-mediated aggregation
EP1599499A2 (en) 2003-02-24 2005-11-30 Dow Global Technologies Inc. Periodic antimicrobial peptides
EP1629012B1 (en) 2003-05-31 2018-11-28 Amgen Research (Munich) GmbH Pharmaceutical compositions comprising bispecific anti-cd3, anti-cd19 antibody constructs for the treatment of b-cell related disorders
EP1633876B1 (en) 2003-06-17 2008-08-20 SemBioSys Genetics Inc. Methods for the production of insulin in plants
CA2545610C (en) 2003-11-19 2014-03-25 Dow Global Technolgies Inc. Auxotrophic pseudomonas fluorescens bacteria for recombinant protein expression
EP2336153B1 (en) 2003-11-21 2016-03-30 Pfenex Inc. Improved expression systems with SEC-system secretion
EP2457577A1 (en) 2003-11-25 2012-05-30 Anjin Corporation Diphtheria toxin variant
CN101151272A (zh) 2003-12-01 2008-03-26 陶氏环球技术公司 在假单胞菌中生产重组二十面体病毒样颗粒
WO2005061712A1 (en) 2003-12-10 2005-07-07 Eli Lilly And Company Muteins of fibroblast growth factor 21
US7235641B2 (en) 2003-12-22 2007-06-26 Micromet Ag Bispecific antibodies
AU2005206951B2 (en) 2004-01-16 2010-08-19 Pfenex Inc. Expression of mammalian proteins in Pseudomonas fluorescens
DE102004014983A1 (de) 2004-03-26 2005-10-20 Univ Stuttgart Rekombinante Polypeptide der Mitglieder der TNF Ligandenfamilie und deren Verwendung
US7384779B2 (en) 2004-04-12 2008-06-10 Corning Incorporated Porous substrate plates and the use thereof
PT1751184E (pt) 2004-05-13 2009-11-10 Lilly Co Eli Proteínas de fusão de fgf-21
WO2005118629A1 (en) 2004-06-02 2005-12-15 Diatech Pty Ltd BINDING MOIETIES BASED ON SHARK IgNAR DOMAINS
AU2005269527B2 (en) * 2004-07-26 2011-12-01 Pfenex Inc. Process for improved protein expression by strain engineering
SI1789442T1 (sl) 2004-09-02 2010-01-29 Lilly Co Eli Proteinske mutante fibroblastnega rastnega faktorja 21
US7563443B2 (en) 2004-09-17 2009-07-21 Domantis Limited Monovalent anti-CD40L antibody polypeptides and compositions thereof
JP5113523B2 (ja) 2004-10-13 2013-01-09 アブリンクス ナームローゼ フェンノートシャップ アミロイド−βに対するナノ抗体及びアルツハイマー病のような神経変性疾患の治療のためのナノ抗体TMを含むポリペプチド
WO2006059701A1 (ja) 2004-12-03 2006-06-08 The University Of Tokushima 微生物または生体分子の収容容器、並びにその作成方法及び使用方法
CA2590820A1 (en) * 2004-12-17 2006-06-22 Nitto Denko Corporation Immobilized degradable cationic polymer for transfecting eukaryotic cells
AU2006205900B8 (en) 2005-01-14 2012-04-05 Ablynx N.V. Methods and assays for distinguishing between different forms of diseases and disorders characterized by thrombocytopenia and/or by spontaneous interaction between Von Willebrand Factor (vWF) and platelets
WO2006079372A1 (en) 2005-01-31 2006-08-03 Ablynx N.V. Method for generating variable domain sequences of heavy chain antibodies
PA8672101A1 (es) 2005-04-29 2006-12-07 Centocor Inc Anticuerpos anti-il-6, composiciones, métodos y usos
EP2949668B1 (en) 2005-05-18 2019-08-14 Ablynx N.V. Improved nanobodies tm against tumor necrosis factor-alpha
EP1907418A2 (en) 2005-07-15 2008-04-09 ZymoGenetics, Inc. Heterodimeric four helix bundle cytokines
JP5134540B2 (ja) 2005-09-19 2013-01-30 アラーガン、インコーポレイテッド クロストリジウム毒素活性化クロストリジウム毒素
US20090252681A1 (en) 2005-10-11 2009-10-08 Ablynx N.V. Nanobodies and Polypeptides Against EGFR and IGF-IR
EP1976880B1 (en) 2005-12-21 2016-07-06 Amgen Research (Munich) GmbH Pharmaceutical compositions with resistance to soluble cea
AU2006328882B2 (en) 2005-12-21 2011-06-16 Amgen Research (Munich) Gmbh Pharmaceutical antibody compositions with resistance to soluble CEA
US7553941B2 (en) 2006-02-03 2009-06-30 Modigene Inc Long-acting polypeptides and methods of producing same
US20070224205A1 (en) 2006-03-07 2007-09-27 Powell Thomas J Compositions that include hemagglutinin, methods of making and methods of use thereof
US20070269422A1 (en) 2006-05-17 2007-11-22 Ablynx N.V. Serum albumin binding proteins with long half-lives
CA2695951A1 (en) 2006-08-10 2008-02-14 Plantechno S.R.L. In-plant production of dimeric and/or oligomeric (comprising three or more units) forms of human apo a-1 protein muteins
EP2086998B1 (en) 2006-10-11 2011-12-07 Ablynx N.V. Amino acid sequences that bind to serum proteins in a manner that is essentially independent of the ph, compounds comprising the same, and use thereof
US20080267949A1 (en) 2006-12-05 2008-10-30 Ablynx N.V. Peptides capable of binding to serum proteins
CN102517280A (zh) * 2007-01-31 2012-06-27 菲尼克斯股份有限公司 用于提高表达的细菌前导序列
EP2142651B1 (en) 2007-04-27 2013-05-22 Pfenex Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
US9580719B2 (en) 2007-04-27 2017-02-28 Pfenex, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
AU2008283991A1 (en) 2007-08-03 2009-02-12 Pfenex, Inc. Translation initiation region sequences for the optimal expression of heterologous proteins
SG186017A1 (en) * 2007-11-30 2012-12-28 Kalobios Pharmaceuticals Inc Antibodies to the pcrv antigen of pseudomonas aeruginosa

Similar Documents

Publication Publication Date Title
JP2010524506A5 (enExample)
EP1829959B1 (en) Recombinant microorganism
Li et al. Metaproteomics reveals major microbial players and their metabolic activities during the blooming period of a marine dinoflagellate Prorocentrum donghaiense
Grasso et al. Signal peptide efficiency: from high-throughput data to prediction and explanation
Kawamoto et al. Proteomic studies of an Antarctic cold-adapted bacterium, Shewanella livingstonensis Ac10, for global identification of cold-inducible proteins
WO2005072129A9 (en) Production of host cells containing mutiple integrating vectors by serial transduction
EP2330200A3 (en) Gene expression technique
van Teeseling et al. A new addition to the cell plan of anammox bacteria:“Candidatus Kuenenia stuttgartiensis” has a protein surface layer as the outermost layer of the cell
WO2004099370A3 (en) NOVEL BACILLUS mHKcel CELLULASE
EP2339016A3 (en) Enhanced protein expression in bacillus
JP2008507294A5 (enExample)
Sorokin et al. Wenzhouxiangella strain AB-CW3, a proteolytic bacterium from hypersaline soda lakes that preys on cells of gram-positive bacteria
RU2008134484A (ru) Новые флуоресцентные белки и способы их применения
Jang et al. Development of bicistronic expression system for the enhanced and reliable production of recombinant proteins in Leuconostoc citreum
WO2008017646A3 (en) Expression of genes from gram negative bacteria in fungi
Arntzen et al. Proteomic investigation of the response of Enterococcus faecalis V583 when cultivated in urine
Gao et al. Exploring the N‐terminal role of a heterologous protein in secreting out of Escherichia coli
Agregán et al. Proteomic advances in cereal and vegetable crops
Huang et al. Editing of the proteolytic system of Lactococcus lactis increases its bioactive potential
DE502007005530D1 (de) Mikroorganismenstamm zur Produktion von rekombinanten Proteinen
Peng et al. Triple deletion of clpC, porB, and mepA enhances production of small ubiquitin-like modifier-N-terminal pro-brain natriuretic peptide in Corynebacterium glutamicum
WO2006053021A3 (en) Methods for engineering polypeptide variants via somatic hypermutation and polypeptides made thereby
WO2004111194A3 (en) Method to increase protein production in culture
Pyati et al. Optimising expression of the recombinant fusion protein biopesticide ω-hexatoxin-Hv1a/GNA in Pichia pastoris: sequence modifications and a simple method for the generation of multi-copy strains
Villeth et al. Comparative proteome analysis of Xanthomonas campestris pv. campestris in the interaction with the susceptible and the resistant cultivars of Brassica oleracea