JP2010517532A5 - - Google Patents
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- JP2010517532A5 JP2010517532A5 JP2009548414A JP2009548414A JP2010517532A5 JP 2010517532 A5 JP2010517532 A5 JP 2010517532A5 JP 2009548414 A JP2009548414 A JP 2009548414A JP 2009548414 A JP2009548414 A JP 2009548414A JP 2010517532 A5 JP2010517532 A5 JP 2010517532A5
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- JP
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- Prior art keywords
- polypeptide
- nucleic acid
- acid molecule
- nucleotide sequence
- seq
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 108090000765 processed proteins & peptides Proteins 0.000 claims description 169
- 229920001184 polypeptide Polymers 0.000 claims description 165
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 165
- 102000039446 nucleic acids Human genes 0.000 claims description 87
- 108020004707 nucleic acids Proteins 0.000 claims description 87
- 150000007523 nucleic acids Chemical class 0.000 claims description 87
- 102000004169 proteins and genes Human genes 0.000 claims description 80
- 108090000623 proteins and genes Proteins 0.000 claims description 80
- 239000002773 nucleotide Substances 0.000 claims description 75
- 125000003729 nucleotide group Chemical group 0.000 claims description 75
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 51
- 210000004027 cell Anatomy 0.000 claims description 43
- 238000000034 method Methods 0.000 claims description 30
- 239000013598 vector Substances 0.000 claims description 29
- 230000003248 secreting effect Effects 0.000 claims description 24
- 241000894006 Bacteria Species 0.000 claims description 11
- 210000001723 extracellular space Anatomy 0.000 claims description 11
- 210000001322 periplasm Anatomy 0.000 claims description 11
- 241000589516 Pseudomonas Species 0.000 claims description 6
- 102000004190 Enzymes Human genes 0.000 claims description 4
- 108090000790 Enzymes Proteins 0.000 claims description 4
- 241000589540 Pseudomonas fluorescens Species 0.000 claims description 4
- 239000013604 expression vector Substances 0.000 claims description 4
- 108020004705 Codon Proteins 0.000 claims description 3
- 230000003834 intracellular effect Effects 0.000 claims description 3
- 108091033319 polynucleotide Proteins 0.000 claims 12
- 102000040430 polynucleotide Human genes 0.000 claims 12
- 239000002157 polynucleotide Substances 0.000 claims 12
- 235000018102 proteins Nutrition 0.000 description 32
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 description 18
- 102000006010 Protein Disulfide-Isomerase Human genes 0.000 description 12
- 108020003519 protein disulfide isomerase Proteins 0.000 description 12
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 10
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 10
- 238000009396 hybridization Methods 0.000 description 10
- 108091026890 Coding region Proteins 0.000 description 7
- 101100278567 Lelliottia amnigena dsbL gene Proteins 0.000 description 7
- 101150009558 dsbA gene Proteins 0.000 description 7
- 101150015101 dsbC gene Proteins 0.000 description 7
- 102000006335 Phosphate-Binding Proteins Human genes 0.000 description 6
- 108010058514 Phosphate-Binding Proteins Proteins 0.000 description 6
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 6
- 108091028043 Nucleic acid sequence Proteins 0.000 description 5
- 230000003399 chemotactic effect Effects 0.000 description 5
- 230000028327 secretion Effects 0.000 description 5
- 241000588724 Escherichia coli Species 0.000 description 4
- 230000001580 bacterial effect Effects 0.000 description 4
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 3
- 241000206602 Eukaryota Species 0.000 description 2
- 108010076504 Protein Sorting Signals Proteins 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 230000008685 targeting Effects 0.000 description 2
- 238000001042 affinity chromatography Methods 0.000 description 1
- 230000035605 chemotaxis Effects 0.000 description 1
- 125000000151 cysteine group Chemical group N[C@@H](CS)C(=O)* 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 102000037865 fusion proteins Human genes 0.000 description 1
- 108020001507 fusion proteins Proteins 0.000 description 1
- 239000000411 inducer Substances 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 229910017053 inorganic salt Inorganic materials 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 108010087967 type I signal peptidase Proteins 0.000 description 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US88747607P | 2007-01-31 | 2007-01-31 | |
| US88748607P | 2007-01-31 | 2007-01-31 | |
| US60/887,476 | 2007-01-31 | ||
| US60/887,486 | 2007-01-31 | ||
| PCT/US2008/052434 WO2008094986A2 (en) | 2007-01-31 | 2008-01-30 | Bacterial leader sequences for increased expression |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015047870A Division JP6495690B2 (ja) | 2007-01-31 | 2015-03-11 | 発現上昇のための細菌リーダー配列 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2010517532A JP2010517532A (ja) | 2010-05-27 |
| JP2010517532A5 true JP2010517532A5 (enExample) | 2014-10-23 |
| JP5714230B2 JP5714230B2 (ja) | 2015-05-07 |
Family
ID=39462488
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2009548414A Expired - Fee Related JP5714230B2 (ja) | 2007-01-31 | 2008-01-30 | 発現上昇のための細菌リーダー配列 |
| JP2015047870A Active JP6495690B2 (ja) | 2007-01-31 | 2015-03-11 | 発現上昇のための細菌リーダー配列 |
| JP2018027221A Pending JP2018102305A (ja) | 2007-01-31 | 2018-02-19 | 発現上昇のための細菌リーダー配列 |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015047870A Active JP6495690B2 (ja) | 2007-01-31 | 2015-03-11 | 発現上昇のための細菌リーダー配列 |
| JP2018027221A Pending JP2018102305A (ja) | 2007-01-31 | 2018-02-19 | 発現上昇のための細菌リーダー配列 |
Country Status (10)
| Country | Link |
|---|---|
| US (3) | US7618799B2 (enExample) |
| EP (2) | EP2468869B1 (enExample) |
| JP (3) | JP5714230B2 (enExample) |
| KR (1) | KR101491867B1 (enExample) |
| CN (2) | CN101641441A (enExample) |
| AU (1) | AU2008210538B2 (enExample) |
| BR (1) | BRPI0807834A2 (enExample) |
| CA (1) | CA2677179C (enExample) |
| PL (1) | PL2468869T3 (enExample) |
| WO (1) | WO2008094986A2 (enExample) |
Families Citing this family (65)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9453251B2 (en) * | 2002-10-08 | 2016-09-27 | Pfenex Inc. | Expression of mammalian proteins in Pseudomonas fluorescens |
| AU2004317306B2 (en) * | 2003-11-21 | 2010-09-02 | Pelican Technology Holdings, Inc. | Improved expression systems with Sec-system secretion |
| JP2008507294A (ja) | 2004-07-26 | 2008-03-13 | ダウ グローバル テクノロジーズ インコーポレイティド | 菌株遺伝子操作による改善されたタンパク質発現のための方法 |
| EP2468869B1 (en) | 2007-01-31 | 2015-03-18 | Pfenex Inc. | Bacterial leader sequences for increased expression |
| US9580719B2 (en) * | 2007-04-27 | 2017-02-28 | Pfenex, Inc. | Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins |
| EP2615172A1 (en) * | 2007-04-27 | 2013-07-17 | Pfenex Inc. | Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins |
| JP5873005B2 (ja) | 2009-04-17 | 2016-03-01 | ダウ アグロサイエンシィズ エルエルシー | Ｄｉｇ−３殺虫性ｃｒｙトキシン |
| US7794969B1 (en) | 2009-07-09 | 2010-09-14 | Joule Unlimited, Inc. | Methods and compositions for the recombinant biosynthesis of n-alkanes |
| US20120077251A1 (en) * | 2010-09-14 | 2012-03-29 | C/O Joule Unlimited Technologies, Inc. | Methods and Compositions for Targeting Heterologous Integral Membrane Proteins to the Cyanobacterial Plasma Membrane |
| JP2013514769A (ja) | 2009-12-16 | 2013-05-02 | ダウ アグロサイエンシィズ エルエルシー | 害虫抵抗性管理のためのＣＲＹ１ＣａおよびＣＲＹ１Ｆａタンパク質の併用 |
| PE20130557A1 (es) * | 2010-03-04 | 2013-05-19 | Pfenex Inc | Metodo para producir proteinas de interferon recombinantes solubles sin desnaturalizacion |
| BR112012024898A2 (pt) | 2010-03-30 | 2015-10-06 | Pfenex Inc | expressão de nível elevado de proteínas recombinantes de toxinas |
| AU2010201410B2 (en) * | 2010-03-30 | 2015-04-30 | Pelican Technology Holdings, Inc. | High level expression of recombinant CRM197 |
| EP2552949B1 (en) * | 2010-04-01 | 2016-08-17 | Pfenex Inc. | Methods for g-csf production in a pseudomonas host cell |
| MA34242B1 (fr) | 2010-04-23 | 2013-05-02 | Dow Agrosciences Llc | Combinaisons incluant les protéines cry34ab/35ab et cry3aa pour prévenir le développement de la résistance chez les chrysoméles des racines du maïs (diabrotica spp.) |
| GB201106225D0 (en) * | 2011-04-13 | 2011-05-25 | Glaxosmithkline Biolog Sa | Fermentation process |
| ES2864764T3 (es) * | 2011-05-11 | 2021-10-14 | Childrens Medical Center | Composición inmunogénica que presenta múltiples antígenos, y métodos y usos de la misma |
| CN103998461B (zh) | 2011-09-08 | 2018-02-02 | 科德克希思公司 | 用于合成取代的内酰胺的生物催化剂和方法 |
| US9815869B2 (en) | 2012-02-09 | 2017-11-14 | University Of Rochester | SP-B and SP-C peptides, synthetic lung surfactants, and use thereof |
| SG11201404991YA (en) | 2012-02-23 | 2014-09-26 | Stage Cell Therapeutics Gmbh | Chromatographic isolation of cells and other complex biological materials |
| US9169304B2 (en) | 2012-05-01 | 2015-10-27 | Pfenex Inc. | Process for purifying recombinant Plasmodium falciparum circumsporozoite protein |
| AR094276A1 (es) * | 2012-12-21 | 2015-07-22 | Alkem Laboratories Ltd | Marcadores de fusion y sistema de vector de expresion para la expresion de hormona paratiroides humana (rhpth) |
| WO2014124228A1 (en) | 2013-02-07 | 2014-08-14 | Children's Medical Center Corporation | Protein antigens that provide protection against pneumococcal colonization and/or disease |
| SI2961844T1 (sl) | 2013-02-28 | 2018-11-30 | Codexis, Inc. | Spremenjeni polipeptidi transaminaze za industrijsko biokatalizo |
| DK2968470T3 (da) | 2013-03-12 | 2021-02-01 | Massachusetts Gen Hospital | Modificeret müllersk inhiberende substans (mis)-proteiner og anvendelser deraf til behandlingen af sygdomme |
| US9744223B2 (en) * | 2013-03-15 | 2017-08-29 | Panacea Pharmaceuticals, Inc. | Therapeutic cancer vaccine targeted to HAAH (aspartyl-[asparaginyl]-β-hydroxylase) |
| RU2013119826A (ru) | 2013-04-29 | 2014-11-10 | Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО "АГРИ") | Коринеформная бактерия и способ получения гетерологичных гибридных белков |
| EP3079712B1 (en) | 2013-12-11 | 2022-02-02 | The General Hospital Corporation | Use of mullerian inhibiting substance (mis) proteins for contraception and ovarian reserve preservation |
| WO2015139046A1 (en) * | 2014-03-14 | 2015-09-17 | Genentech, Inc. | Methods and compositions for secretion of heterologous polypeptides |
| TW201542093A (zh) | 2014-03-21 | 2015-11-16 | 艾格里遺傳學股份有限公司 | 用於控制玉米穗蟲之Ｃｒｙ１Ｄ |
| KR101660580B1 (ko) * | 2014-04-02 | 2016-09-28 | 프레스티지 바이오파마 피티이. 엘티디. | 항체의 당 함량 조절을 통한 항체의 제조 방법 |
| AU2015277433B2 (en) | 2014-06-20 | 2017-05-11 | Dow Agrosciences Llc | Vegetative insecticidal proteins useful for control of insect pests |
| US9616114B1 (en) | 2014-09-18 | 2017-04-11 | David Gordon Bermudes | Modified bacteria having improved pharmacokinetics and tumor colonization enhancing antitumor activity |
| CN113444183B (zh) | 2014-12-01 | 2025-06-17 | 佩利肯科技控股公司 | 用于肽生产的融合伴侣 |
| CN114213510B (zh) | 2014-12-30 | 2025-03-18 | 美国陶氏益农公司 | 可用于控制昆虫害虫的修饰的Cry1Ca毒素 |
| CN107532163A (zh) * | 2015-04-24 | 2018-01-02 | 味之素株式会社 | 蛋白质的分泌生产方法 |
| MX2017014002A (es) | 2015-05-01 | 2018-08-01 | Inhibrx Lp | Moléculas de direccionamiento del sistema de secreción tipo iii . |
| US10676723B2 (en) | 2015-05-11 | 2020-06-09 | David Gordon Bermudes | Chimeric protein toxins for expression by therapeutic bacteria |
| MA45489A (fr) | 2015-10-22 | 2018-08-29 | Juno Therapeutics Gmbh | Procédés de culture de cellules, kits et appareil associés |
| KR20180108567A (ko) | 2015-10-22 | 2018-10-04 | 주노 테라퓨틱스 게엠베하 | 형질도입을 위한 방법, 키트, 제제 및 장치 |
| KR102312903B1 (ko) * | 2016-03-31 | 2021-10-15 | 에트리스 게엠베하 | 신규의 최소 utr 서열 |
| US10889830B2 (en) | 2016-09-30 | 2021-01-12 | Dow Agrosciences Llc | Binary insecticidal Cry toxins |
| US11180535B1 (en) | 2016-12-07 | 2021-11-23 | David Gordon Bermudes | Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria |
| US11129906B1 (en) | 2016-12-07 | 2021-09-28 | David Gordon Bermudes | Chimeric protein toxins for expression by therapeutic bacteria |
| CA3046824A1 (en) * | 2016-12-14 | 2018-06-21 | Wageningen Universiteit | Thermostable cas9 nucleases |
| EA035353B1 (ru) * | 2017-02-28 | 2020-06-01 | Акционерное Общество "Биоамид" | Способ получения l-аспарагиновой кислоты и рекомбинантный штамм-продуцент аспартазы escherichia coli |
| CN110730670A (zh) | 2017-03-28 | 2020-01-24 | 儿童医疗中心有限公司 | 基于多抗原提呈系统(maps)的金黄色葡萄球菌疫苗、免疫原性组合物以及它们的用途 |
| IL270142B2 (en) | 2017-04-27 | 2025-05-01 | Juno Therapeutics Gmbh | Oligomeric particle reagents and methods of use thereof |
| JP2020525540A (ja) | 2017-06-23 | 2020-08-27 | ノソコミアル ヴァクスィーン コーポレイション | 免疫原性組成物 |
| AU2018354067B2 (en) | 2017-10-27 | 2023-03-30 | Pfenex Inc. | Method for production of recombinant Erwinia asparaginase |
| AU2018354069A1 (en) * | 2017-10-27 | 2020-06-11 | Pelican Technology Holdings, Inc. | Bacterial leader sequences for periplasmic protein expression |
| WO2019083794A1 (en) | 2017-10-27 | 2019-05-02 | Pfenex Inc. | PROCESS FOR PRODUCING EAR ASPARAGINASE. RECOMBINED COLI |
| CN111373033A (zh) * | 2017-11-23 | 2020-07-03 | 豪夫迈·罗氏有限公司 | 脯氨酸羟化酶及其相关用途、方法和产品 |
| KR101946789B1 (ko) * | 2018-01-26 | 2019-02-12 | (주)휴온스 | 이황화결합 이성질화효소 신호 펩타이드를 포함하는 재조합 벡터 및 이의 용도 |
| CN110655560A (zh) * | 2018-06-29 | 2020-01-07 | 复旦大学 | T7噬菌体尾部纤维蛋白多肽及其应用 |
| JP7535791B2 (ja) | 2018-09-12 | 2024-08-19 | ザ チルドレンズ メディカル センター コーポレーション | 肺炎球菌融合タンパク質ワクチン |
| JP2021535921A (ja) | 2018-09-12 | 2021-12-23 | アフィニバックス、インコーポレイテッド | 多価肺炎球菌ワクチン |
| EP3893870A1 (en) | 2018-12-13 | 2021-10-20 | Huya Bioscience International LLC | Sulcardine administration for treatment of acute atrial fibrillation |
| JP7487951B2 (ja) * | 2019-02-24 | 2024-05-21 | オンコシミス バイオテック プライベート リミテッド | 細菌による組換えｇｌｐ－１ペプチドの連続生産方法 |
| US11471497B1 (en) | 2019-03-13 | 2022-10-18 | David Gordon Bermudes | Copper chelation therapeutics |
| US10973908B1 (en) | 2020-05-14 | 2021-04-13 | David Gordon Bermudes | Expression of SARS-CoV-2 spike protein receptor binding domain in attenuated salmonella as a vaccine |
| WO2022211829A1 (en) | 2021-03-30 | 2022-10-06 | Jazz Pharmaceuticals Ireland Ltd. | Dosing of recombinant l-asparaginase |
| WO2023039223A1 (en) | 2021-09-09 | 2023-03-16 | Affinivax, Inc. | Multivalent pneumococcal vaccines |
| KR102527754B1 (ko) | 2022-07-07 | 2023-04-28 | 배성규 | 필터정화기능을 구비한 초기우수처리장치 |
| CN120349035B (zh) * | 2025-06-25 | 2025-11-11 | 上海明诺环境科技有限公司 | 一种聚甲醛废水处理方法及应用 |
Family Cites Families (69)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4551433A (en) | 1981-05-18 | 1985-11-05 | Genentech, Inc. | Microbial hybrid promoters |
| US4595658A (en) | 1982-09-13 | 1986-06-17 | The Rockefeller University | Method for facilitating externalization of proteins synthesized in bacteria |
| US4511503A (en) | 1982-12-22 | 1985-04-16 | Genentech, Inc. | Purification and activity assurance of precipitated heterologous proteins |
| US4513600A (en) | 1983-01-03 | 1985-04-30 | The Minster Machine Company | Cam actuated ejector for a shell press |
| GB8308483D0 (en) | 1983-03-28 | 1983-05-05 | Health Lab Service Board | Secretion of gene products |
| US4755465A (en) | 1983-04-25 | 1988-07-05 | Genentech, Inc. | Secretion of correctly processed human growth hormone in E. coli and Pseudomonas |
| US4680264A (en) | 1983-07-01 | 1987-07-14 | Lubrizol Genetics, Inc. | Class II mobilizable gram-negative plasmid |
| US5281532A (en) | 1983-07-27 | 1994-01-25 | Mycogen Corporation | Pseudomas hosts transformed with bacillus endotoxin genes |
| US4637980A (en) | 1983-08-09 | 1987-01-20 | Smithkline Beckman Corporation | Externalization of products of bacteria |
| US5288852A (en) | 1984-03-06 | 1994-02-22 | Dainippon Pharmaceutical Co., Ltd. | Human tumor necrosis factor polypeptides |
| EP0155189A3 (en) | 1984-03-16 | 1987-09-16 | Genentech, Inc. | Expression of cell wall degrading proteins and host cells harboring dna encoding such protein |
| US4963495A (en) | 1984-10-05 | 1990-10-16 | Genentech, Inc. | Secretion of heterologous proteins |
| ATE78515T1 (de) | 1984-10-05 | 1992-08-15 | Genentech Inc | Dna, zellkulturen und verfahren zur sekretion von heterologen proteinen und periplasmische proteinrueckgewinnung. |
| US4695462A (en) | 1985-06-28 | 1987-09-22 | Mycogen Corporation | Cellular encapsulation of biological pesticides |
| US4695455A (en) | 1985-01-22 | 1987-09-22 | Mycogen Corporation | Cellular encapsulation of pesticides produced by expression of heterologous genes |
| GB8517071D0 (en) | 1985-07-05 | 1985-08-14 | Hoffmann La Roche | Gram-positive expression control sequence |
| US5618920A (en) | 1985-11-01 | 1997-04-08 | Xoma Corporation | Modular assembly of antibody genes, antibodies prepared thereby and use |
| GB8529014D0 (en) | 1985-11-25 | 1986-01-02 | Biogen Nv | Enhanced secretion of heterologous proteins |
| US5232840A (en) | 1986-03-27 | 1993-08-03 | Monsanto Company | Enhanced protein production in bacteria by employing a novel ribosome binding site |
| AU607209B2 (en) | 1987-04-23 | 1991-02-28 | Monsanto Technology Llc | Secretion of insulin-like growth factor-i in e. coli |
| US5084384A (en) | 1987-04-23 | 1992-01-28 | Monsanto Company | Secretion of insulin-like growth factor-I |
| US5128130A (en) | 1988-01-22 | 1992-07-07 | Mycogen Corporation | Hybrid Bacillus thuringiensis gene, plasmid and transformed Pseudomonas fluorescens |
| US5055294A (en) | 1988-03-03 | 1991-10-08 | Mycogen Corporation | Chimeric bacillus thuringiensis crystal protein gene comprising hd-73 and berliner 1715 toxin genes, transformed and expressed in pseudomonas fluorescens |
| WO1989010971A1 (en) | 1988-05-09 | 1989-11-16 | The United States Of America, As Represented By Th | Vector for secretion of proteins directly into periplasm or culture medium |
| US5169772A (en) | 1988-06-06 | 1992-12-08 | Massachusetts Institute Of Technology | Large scale method for purification of high purity heparinase from flavobacterium heparinum |
| US5175099A (en) | 1989-05-17 | 1992-12-29 | Research Corporation Technologies, Inc. | Retrovirus-mediated secretion of recombinant products |
| US5169760A (en) | 1989-07-27 | 1992-12-08 | Mycogen Corporation | Method, vectors, and host cells for the control of expression of heterologous genes from lac operated promoters |
| AU631719B2 (en) | 1989-12-15 | 1992-12-03 | W.R. Grace & Co.-Conn. | Autodeposition emulsion for selectively protecting metallic surfaces |
| US5641671A (en) | 1990-07-06 | 1997-06-24 | Unilever Patent Holdings B.V. | Production of active Pseudomonas glumae lipase in homologous or heterologous hosts |
| FI913434A7 (fi) | 1990-07-16 | 1992-01-17 | Technical Research Centre F Finland | Rekombinanta fusionsproteiner vilka utsoendras. |
| CA2052852A1 (en) | 1990-10-30 | 1992-05-01 | Carl Rande Shervin | Method of determining blend time in stirred tanks |
| JP3014434B2 (ja) | 1990-10-31 | 2000-02-28 | 昭和アルミニウム株式会社 | 熱交換器 |
| US5417971A (en) | 1991-10-22 | 1995-05-23 | University Of Saskatchewan | Vaccines for Actinobacillus pleuropneumoniae |
| US5348867A (en) | 1991-11-15 | 1994-09-20 | George Georgiou | Expression of proteins on bacterial surface |
| DE69429858D1 (de) * | 1993-04-09 | 2002-03-21 | Bio Technology General Corp | Herstellung eines rekombinanten inhibitors des faktors xa des blutegels hirudo medicinalis |
| US5914254A (en) | 1993-08-02 | 1999-06-22 | Celtrix Pharmaceuticals, Inc. | Expression of fusion polypeptides transported out of the cytoplasm without leader sequences |
| US5563046A (en) * | 1993-08-02 | 1996-10-08 | Celtrix Pharmaceuticals, Inc. | Fusion polypeptides and proteins |
| US5527883A (en) | 1994-05-06 | 1996-06-18 | Mycogen Corporation | Delta-endotoxin expression in pseudomonas fluorescens |
| EP0797671B1 (en) | 1994-12-09 | 2004-03-03 | Novo Nordisk A/S | A process of producing extracellular proteins in bacteria |
| US6495357B1 (en) | 1995-07-14 | 2002-12-17 | Novozyme A/S | Lipolytic enzymes |
| IT1283225B1 (it) | 1996-03-11 | 1998-04-16 | Renata Maria Anna Ve Cavaliere | Ceppi di batteri, composizione farmaceutica contenente uno o piu' di tali ceppi e uso dei medesimi per la prevenzione e la terapia delle |
| US6361989B1 (en) | 1997-10-13 | 2002-03-26 | Novozymes A/S | α-amylase and α-amylase variants |
| US6156552A (en) | 1998-02-18 | 2000-12-05 | Novo Nordisk A/S | Lipase variants |
| US20030064435A1 (en) | 1998-05-28 | 2003-04-03 | Weiner Joel Hirsch | Compositions and methods for protein secretion |
| US6323007B1 (en) | 1998-09-18 | 2001-11-27 | Novozymes A/S | 2,6-β-D-fructan hydrolase enzyme and processes for using the enzyme |
| WO2000024873A1 (en) | 1998-10-28 | 2000-05-04 | Genentech, Inc. | Process for recovering heterologous polypeptides from bacterial cells |
| KR100312456B1 (ko) | 1999-03-13 | 2001-11-03 | 윤덕용 | 슈도모나스 플루오레슨스 유래의 외래단백질 분비촉진유전자 |
| BR0009393A (pt) | 1999-03-29 | 2002-01-08 | Novozymes As | Polipeptìdeo, sequência de ácido nucleico isolada, construção de ácido nucleico, vetor de expressão recombinante, célula hospedeira recombinante, método para a produção do polipeptìdeo, uso de um polipeptìdeo, e, composição detergente |
| US6664386B1 (en) | 1999-04-08 | 2003-12-16 | The Research Foundation Of State University Of New York | System for efficient secretion of recombinant proteins |
| US6558939B1 (en) | 1999-08-31 | 2003-05-06 | Novozymes, A/S | Proteases and variants thereof |
| DE19944870A1 (de) | 1999-09-18 | 2001-03-29 | Aventis Pharma Gmbh | Signalsequenzen zur Herstellung von Leu-Hirudin über Sekretion durch E. coli in das Kulturmedium |
| FI107081B (fi) * | 1999-10-19 | 2001-05-31 | Abb Substation Automation Oy | Menetelmä ja järjestely osittaispurkauslähteiden lukumäärän selvittämiseksi |
| US6617143B1 (en) | 1999-10-20 | 2003-09-09 | Novozymes A/S | Polypeptides having glucanotransferase activity and nucleic acids encoding same |
| US6509181B1 (en) | 2000-04-14 | 2003-01-21 | Novozymes, A/S | Polypeptides having haloperoxide activity |
| US7087412B2 (en) | 2000-11-14 | 2006-08-08 | Boehringer Ingelheim International Gmbh | Methods for large scale protein production in prokaryotes |
| GB0027782D0 (en) | 2000-11-14 | 2000-12-27 | Boehringer Ingelheim Int | Methods fro large scale protein productio in prokaryotes |
| US7202059B2 (en) | 2001-02-20 | 2007-04-10 | Sanofi-Aventis Deutschland Gmbh | Fusion proteins capable of being secreted into a fermentation medium |
| DE10108212A1 (de) | 2001-02-20 | 2002-08-22 | Aventis Pharma Gmbh | Fusionsprotein zur Sekretion von Wertprotein in bakterielle Überstände |
| US7419783B2 (en) | 2001-11-05 | 2008-09-02 | Research Development Foundation | Engineering of leader peptides for the secretion of recombinant proteins in bacteria |
| AU2002306484A1 (en) | 2002-02-13 | 2003-09-04 | Dow Global Technologies Inc. | Over-expression of extremozyme genes in pseudomonads and closely related bacteria |
| WO2003079007A1 (en) | 2002-03-15 | 2003-09-25 | The Trustees Of The University Of Pennsylvania | Heterologous protein production using the twin arginine translocation pathway |
| WO2003089455A2 (en) | 2002-04-22 | 2003-10-30 | Dow Global Technologies Inc. | Low-cost production of peptides |
| JP2006506049A (ja) | 2002-07-03 | 2006-02-23 | ダウ グローバル テクノロジーズ インコーポレイティド | ベンゾエート−及びアントラニレート−誘導性プロモーター |
| AU2004317306B2 (en) | 2003-11-21 | 2010-09-02 | Pelican Technology Holdings, Inc. | Improved expression systems with Sec-system secretion |
| AU2005206951B2 (en) | 2004-01-16 | 2010-08-19 | Pfenex Inc. | Expression of mammalian proteins in Pseudomonas fluorescens |
| AU2006268780B2 (en) * | 2005-07-08 | 2011-03-24 | University Of Zurich | Phage display using cotranslational translocation of fusion polypeptides |
| WO2007142954A2 (en) * | 2006-05-30 | 2007-12-13 | Dow Global Technologies Inc. | Codon optimization method |
| EP2468869B1 (en) * | 2007-01-31 | 2015-03-18 | Pfenex Inc. | Bacterial leader sequences for increased expression |
| EP2615172A1 (en) * | 2007-04-27 | 2013-07-17 | Pfenex Inc. | Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins |
-
2008
- 2008-01-30 EP EP11194072.2A patent/EP2468869B1/en active Active
- 2008-01-30 CN CN200880009677A patent/CN101641441A/zh active Pending
- 2008-01-30 CN CN2011103821798A patent/CN102517280A/zh active Pending
- 2008-01-30 KR KR1020097017960A patent/KR101491867B1/ko active Active
- 2008-01-30 PL PL11194072T patent/PL2468869T3/pl unknown
- 2008-01-30 JP JP2009548414A patent/JP5714230B2/ja not_active Expired - Fee Related
- 2008-01-30 BR BRPI0807834-3A patent/BRPI0807834A2/pt not_active IP Right Cessation
- 2008-01-30 EP EP08714119A patent/EP2108047B1/en active Active
- 2008-01-30 AU AU2008210538A patent/AU2008210538B2/en active Active
- 2008-01-30 US US12/022,789 patent/US7618799B2/en active Active
- 2008-01-30 CA CA2677179A patent/CA2677179C/en active Active
- 2008-01-30 WO PCT/US2008/052434 patent/WO2008094986A2/en not_active Ceased
-
2009
- 2009-10-22 US US12/604,061 patent/US7833752B2/en active Active
-
2010
- 2010-09-16 US US12/884,095 patent/US20110020868A1/en not_active Abandoned
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2015
- 2015-03-11 JP JP2015047870A patent/JP6495690B2/ja active Active
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2018
- 2018-02-19 JP JP2018027221A patent/JP2018102305A/ja active Pending
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