PL2468869T3 - Bakteryjna sekwencja wiodąca dla zwiększonej ekspresji - Google Patents

Bakteryjna sekwencja wiodąca dla zwiększonej ekspresji

Info

Publication number
PL2468869T3
PL2468869T3 PL11194072T PL11194072T PL2468869T3 PL 2468869 T3 PL2468869 T3 PL 2468869T3 PL 11194072 T PL11194072 T PL 11194072T PL 11194072 T PL11194072 T PL 11194072T PL 2468869 T3 PL2468869 T3 PL 2468869T3
Authority
PL
Poland
Prior art keywords
increased expression
leader sequences
bacterial leader
bacterial
sequences
Prior art date
Application number
PL11194072T
Other languages
English (en)
Polish (pl)
Inventor
Russell John Coleman
Diane M Retallack
Thomas M Ramseier
Charles Douglas Hershberger
Stacey L Lee
Original Assignee
Pfenex Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pfenex Inc filed Critical Pfenex Inc
Publication of PL2468869T3 publication Critical patent/PL2468869T3/pl

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
PL11194072T 2007-01-31 2008-01-30 Bakteryjna sekwencja wiodąca dla zwiększonej ekspresji PL2468869T3 (pl)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US88747607P 2007-01-31 2007-01-31
US88748607P 2007-01-31 2007-01-31
EP11194072.2A EP2468869B1 (en) 2007-01-31 2008-01-30 Bacterial leader sequences for increased expression
EP08714119A EP2108047B1 (en) 2007-01-31 2008-01-30 Bacterial leader sequences for increased expression

Publications (1)

Publication Number Publication Date
PL2468869T3 true PL2468869T3 (pl) 2015-08-31

Family

ID=39462488

Family Applications (1)

Application Number Title Priority Date Filing Date
PL11194072T PL2468869T3 (pl) 2007-01-31 2008-01-30 Bakteryjna sekwencja wiodąca dla zwiększonej ekspresji

Country Status (10)

Country Link
US (3) US7618799B2 (enExample)
EP (2) EP2468869B1 (enExample)
JP (3) JP5714230B2 (enExample)
KR (1) KR101491867B1 (enExample)
CN (2) CN101641441A (enExample)
AU (1) AU2008210538B2 (enExample)
BR (1) BRPI0807834A2 (enExample)
CA (1) CA2677179C (enExample)
PL (1) PL2468869T3 (enExample)
WO (1) WO2008094986A2 (enExample)

Families Citing this family (65)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9453251B2 (en) * 2002-10-08 2016-09-27 Pfenex Inc. Expression of mammalian proteins in Pseudomonas fluorescens
AU2004317306B2 (en) * 2003-11-21 2010-09-02 Pelican Technology Holdings, Inc. Improved expression systems with Sec-system secretion
JP2008507294A (ja) 2004-07-26 2008-03-13 ダウ グローバル テクノロジーズ インコーポレイティド 菌株遺伝子操作による改善されたタンパク質発現のための方法
EP2468869B1 (en) 2007-01-31 2015-03-18 Pfenex Inc. Bacterial leader sequences for increased expression
US9580719B2 (en) * 2007-04-27 2017-02-28 Pfenex, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
EP2615172A1 (en) * 2007-04-27 2013-07-17 Pfenex Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
JP5873005B2 (ja) 2009-04-17 2016-03-01 ダウ アグロサイエンシィズ エルエルシー Ｄｉｇ−３殺虫性ｃｒｙトキシン
US7794969B1 (en) 2009-07-09 2010-09-14 Joule Unlimited, Inc. Methods and compositions for the recombinant biosynthesis of n-alkanes
US20120077251A1 (en) * 2010-09-14 2012-03-29 C/O Joule Unlimited Technologies, Inc. Methods and Compositions for Targeting Heterologous Integral Membrane Proteins to the Cyanobacterial Plasma Membrane
JP2013514769A (ja) 2009-12-16 2013-05-02 ダウ アグロサイエンシィズ エルエルシー 害虫抵抗性管理のためのＣＲＹ１ＣａおよびＣＲＹ１Ｆａタンパク質の併用
PE20130557A1 (es) * 2010-03-04 2013-05-19 Pfenex Inc Metodo para producir proteinas de interferon recombinantes solubles sin desnaturalizacion
BR112012024898A2 (pt) 2010-03-30 2015-10-06 Pfenex Inc expressão de nível elevado de proteínas recombinantes de toxinas
AU2010201410B2 (en) * 2010-03-30 2015-04-30 Pelican Technology Holdings, Inc. High level expression of recombinant CRM197
EP2552949B1 (en) * 2010-04-01 2016-08-17 Pfenex Inc. Methods for g-csf production in a pseudomonas host cell
MA34242B1 (fr) 2010-04-23 2013-05-02 Dow Agrosciences Llc Combinaisons incluant les protéines cry34ab/35ab et cry3aa pour prévenir le développement de la résistance chez les chrysoméles des racines du maïs (diabrotica spp.)
GB201106225D0 (en) * 2011-04-13 2011-05-25 Glaxosmithkline Biolog Sa Fermentation process
ES2864764T3 (es) * 2011-05-11 2021-10-14 Childrens Medical Center Composición inmunogénica que presenta múltiples antígenos, y métodos y usos de la misma
CN103998461B (zh) 2011-09-08 2018-02-02 科德克希思公司 用于合成取代的内酰胺的生物催化剂和方法
US9815869B2 (en) 2012-02-09 2017-11-14 University Of Rochester SP-B and SP-C peptides, synthetic lung surfactants, and use thereof
SG11201404991YA (en) 2012-02-23 2014-09-26 Stage Cell Therapeutics Gmbh Chromatographic isolation of cells and other complex biological materials
US9169304B2 (en) 2012-05-01 2015-10-27 Pfenex Inc. Process for purifying recombinant Plasmodium falciparum circumsporozoite protein
AR094276A1 (es) * 2012-12-21 2015-07-22 Alkem Laboratories Ltd Marcadores de fusion y sistema de vector de expresion para la expresion de hormona paratiroides humana (rhpth)
WO2014124228A1 (en) 2013-02-07 2014-08-14 Children's Medical Center Corporation Protein antigens that provide protection against pneumococcal colonization and/or disease
SI2961844T1 (sl) 2013-02-28 2018-11-30 Codexis, Inc. Spremenjeni polipeptidi transaminaze za industrijsko biokatalizo
DK2968470T3 (da) 2013-03-12 2021-02-01 Massachusetts Gen Hospital Modificeret müllersk inhiberende substans (mis)-proteiner og anvendelser deraf til behandlingen af sygdomme
US9744223B2 (en) * 2013-03-15 2017-08-29 Panacea Pharmaceuticals, Inc. Therapeutic cancer vaccine targeted to HAAH (aspartyl-[asparaginyl]-β-hydroxylase)
RU2013119826A (ru) 2013-04-29 2014-11-10 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО "АГРИ") Коринеформная бактерия и способ получения гетерологичных гибридных белков
EP3079712B1 (en) 2013-12-11 2022-02-02 The General Hospital Corporation Use of mullerian inhibiting substance (mis) proteins for contraception and ovarian reserve preservation
WO2015139046A1 (en) * 2014-03-14 2015-09-17 Genentech, Inc. Methods and compositions for secretion of heterologous polypeptides
TW201542093A (zh) 2014-03-21 2015-11-16 艾格里遺傳學股份有限公司 用於控制玉米穗蟲之Ｃｒｙ１Ｄ
KR101660580B1 (ko) * 2014-04-02 2016-09-28 프레스티지 바이오파마 피티이. 엘티디. 항체의 당 함량 조절을 통한 항체의 제조 방법
AU2015277433B2 (en) 2014-06-20 2017-05-11 Dow Agrosciences Llc Vegetative insecticidal proteins useful for control of insect pests
US9616114B1 (en) 2014-09-18 2017-04-11 David Gordon Bermudes Modified bacteria having improved pharmacokinetics and tumor colonization enhancing antitumor activity
CN113444183B (zh) 2014-12-01 2025-06-17 佩利肯科技控股公司 用于肽生产的融合伴侣
CN114213510B (zh) 2014-12-30 2025-03-18 美国陶氏益农公司 可用于控制昆虫害虫的修饰的Cry1Ca毒素
CN107532163A (zh) * 2015-04-24 2018-01-02 味之素株式会社 蛋白质的分泌生产方法
MX2017014002A (es) 2015-05-01 2018-08-01 Inhibrx Lp Moléculas de direccionamiento del sistema de secreción tipo iii .
US10676723B2 (en) 2015-05-11 2020-06-09 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
MA45489A (fr) 2015-10-22 2018-08-29 Juno Therapeutics Gmbh Procédés de culture de cellules, kits et appareil associés
KR20180108567A (ko) 2015-10-22 2018-10-04 주노 테라퓨틱스 게엠베하 형질도입을 위한 방법, 키트, 제제 및 장치
KR102312903B1 (ko) * 2016-03-31 2021-10-15 에트리스 게엠베하 신규의 최소 utr 서열
US10889830B2 (en) 2016-09-30 2021-01-12 Dow Agrosciences Llc Binary insecticidal Cry toxins
US11180535B1 (en) 2016-12-07 2021-11-23 David Gordon Bermudes Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
CA3046824A1 (en) * 2016-12-14 2018-06-21 Wageningen Universiteit Thermostable cas9 nucleases
EA035353B1 (ru) * 2017-02-28 2020-06-01 Акционерное Общество "Биоамид" Способ получения l-аспарагиновой кислоты и рекомбинантный штамм-продуцент аспартазы escherichia coli
CN110730670A (zh) 2017-03-28 2020-01-24 儿童医疗中心有限公司 基于多抗原提呈系统(maps)的金黄色葡萄球菌疫苗、免疫原性组合物以及它们的用途
IL270142B2 (en) 2017-04-27 2025-05-01 Juno Therapeutics Gmbh Oligomeric particle reagents and methods of use thereof
JP2020525540A (ja) 2017-06-23 2020-08-27 ノソコミアル ヴァクスィーン コーポレイション 免疫原性組成物
AU2018354067B2 (en) 2017-10-27 2023-03-30 Pfenex Inc. Method for production of recombinant Erwinia asparaginase
AU2018354069A1 (en) * 2017-10-27 2020-06-11 Pelican Technology Holdings, Inc. Bacterial leader sequences for periplasmic protein expression
WO2019083794A1 (en) 2017-10-27 2019-05-02 Pfenex Inc. PROCESS FOR PRODUCING EAR ASPARAGINASE. RECOMBINED COLI
CN111373033A (zh) * 2017-11-23 2020-07-03 豪夫迈·罗氏有限公司 脯氨酸羟化酶及其相关用途、方法和产品
KR101946789B1 (ko) * 2018-01-26 2019-02-12 (주)휴온스 이황화결합 이성질화효소 신호 펩타이드를 포함하는 재조합 벡터 및 이의 용도
CN110655560A (zh) * 2018-06-29 2020-01-07 复旦大学 T7噬菌体尾部纤维蛋白多肽及其应用
JP7535791B2 (ja) 2018-09-12 2024-08-19 ザ チルドレンズ メディカル センター コーポレーション 肺炎球菌融合タンパク質ワクチン
JP2021535921A (ja) 2018-09-12 2021-12-23 アフィニバックス、インコーポレイテッド 多価肺炎球菌ワクチン
EP3893870A1 (en) 2018-12-13 2021-10-20 Huya Bioscience International LLC Sulcardine administration for treatment of acute atrial fibrillation
JP7487951B2 (ja) * 2019-02-24 2024-05-21 オンコシミス バイオテック プライベート リミテッド 細菌による組換えｇｌｐ－１ペプチドの連続生産方法
US11471497B1 (en) 2019-03-13 2022-10-18 David Gordon Bermudes Copper chelation therapeutics
US10973908B1 (en) 2020-05-14 2021-04-13 David Gordon Bermudes Expression of SARS-CoV-2 spike protein receptor binding domain in attenuated salmonella as a vaccine
WO2022211829A1 (en) 2021-03-30 2022-10-06 Jazz Pharmaceuticals Ireland Ltd. Dosing of recombinant l-asparaginase
WO2023039223A1 (en) 2021-09-09 2023-03-16 Affinivax, Inc. Multivalent pneumococcal vaccines
KR102527754B1 (ko) 2022-07-07 2023-04-28 배성규 필터정화기능을 구비한 초기우수처리장치
CN120349035B (zh) * 2025-06-25 2025-11-11 上海明诺环境科技有限公司 一种聚甲醛废水处理方法及应用

Family Cites Families (69)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4551433A (en) 1981-05-18 1985-11-05 Genentech, Inc. Microbial hybrid promoters
US4595658A (en) 1982-09-13 1986-06-17 The Rockefeller University Method for facilitating externalization of proteins synthesized in bacteria
US4511503A (en) 1982-12-22 1985-04-16 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4513600A (en) 1983-01-03 1985-04-30 The Minster Machine Company Cam actuated ejector for a shell press
GB8308483D0 (en) 1983-03-28 1983-05-05 Health Lab Service Board Secretion of gene products
US4755465A (en) 1983-04-25 1988-07-05 Genentech, Inc. Secretion of correctly processed human growth hormone in E. coli and Pseudomonas
US4680264A (en) 1983-07-01 1987-07-14 Lubrizol Genetics, Inc. Class II mobilizable gram-negative plasmid
US5281532A (en) 1983-07-27 1994-01-25 Mycogen Corporation Pseudomas hosts transformed with bacillus endotoxin genes
US4637980A (en) 1983-08-09 1987-01-20 Smithkline Beckman Corporation Externalization of products of bacteria
US5288852A (en) 1984-03-06 1994-02-22 Dainippon Pharmaceutical Co., Ltd. Human tumor necrosis factor polypeptides
EP0155189A3 (en) 1984-03-16 1987-09-16 Genentech, Inc. Expression of cell wall degrading proteins and host cells harboring dna encoding such protein
US4963495A (en) 1984-10-05 1990-10-16 Genentech, Inc. Secretion of heterologous proteins
ATE78515T1 (de) 1984-10-05 1992-08-15 Genentech Inc Dna, zellkulturen und verfahren zur sekretion von heterologen proteinen und periplasmische proteinrueckgewinnung.
US4695462A (en) 1985-06-28 1987-09-22 Mycogen Corporation Cellular encapsulation of biological pesticides
US4695455A (en) 1985-01-22 1987-09-22 Mycogen Corporation Cellular encapsulation of pesticides produced by expression of heterologous genes
GB8517071D0 (en) 1985-07-05 1985-08-14 Hoffmann La Roche Gram-positive expression control sequence
US5618920A (en) 1985-11-01 1997-04-08 Xoma Corporation Modular assembly of antibody genes, antibodies prepared thereby and use
GB8529014D0 (en) 1985-11-25 1986-01-02 Biogen Nv Enhanced secretion of heterologous proteins
US5232840A (en) 1986-03-27 1993-08-03 Monsanto Company Enhanced protein production in bacteria by employing a novel ribosome binding site
AU607209B2 (en) 1987-04-23 1991-02-28 Monsanto Technology Llc Secretion of insulin-like growth factor-i in e. coli
US5084384A (en) 1987-04-23 1992-01-28 Monsanto Company Secretion of insulin-like growth factor-I
US5128130A (en) 1988-01-22 1992-07-07 Mycogen Corporation Hybrid Bacillus thuringiensis gene, plasmid and transformed Pseudomonas fluorescens
US5055294A (en) 1988-03-03 1991-10-08 Mycogen Corporation Chimeric bacillus thuringiensis crystal protein gene comprising hd-73 and berliner 1715 toxin genes, transformed and expressed in pseudomonas fluorescens
WO1989010971A1 (en) 1988-05-09 1989-11-16 The United States Of America, As Represented By Th Vector for secretion of proteins directly into periplasm or culture medium
US5169772A (en) 1988-06-06 1992-12-08 Massachusetts Institute Of Technology Large scale method for purification of high purity heparinase from flavobacterium heparinum
US5175099A (en) 1989-05-17 1992-12-29 Research Corporation Technologies, Inc. Retrovirus-mediated secretion of recombinant products
US5169760A (en) 1989-07-27 1992-12-08 Mycogen Corporation Method, vectors, and host cells for the control of expression of heterologous genes from lac operated promoters
AU631719B2 (en) 1989-12-15 1992-12-03 W.R. Grace & Co.-Conn. Autodeposition emulsion for selectively protecting metallic surfaces
US5641671A (en) 1990-07-06 1997-06-24 Unilever Patent Holdings B.V. Production of active Pseudomonas glumae lipase in homologous or heterologous hosts
FI913434A7 (fi) 1990-07-16 1992-01-17 Technical Research Centre F Finland Rekombinanta fusionsproteiner vilka utsoendras.
CA2052852A1 (en) 1990-10-30 1992-05-01 Carl Rande Shervin Method of determining blend time in stirred tanks
JP3014434B2 (ja) 1990-10-31 2000-02-28 昭和アルミニウム株式会社 熱交換器
US5417971A (en) 1991-10-22 1995-05-23 University Of Saskatchewan Vaccines for Actinobacillus pleuropneumoniae
US5348867A (en) 1991-11-15 1994-09-20 George Georgiou Expression of proteins on bacterial surface
DE69429858D1 (de) * 1993-04-09 2002-03-21 Bio Technology General Corp Herstellung eines rekombinanten inhibitors des faktors xa des blutegels hirudo medicinalis
US5914254A (en) 1993-08-02 1999-06-22 Celtrix Pharmaceuticals, Inc. Expression of fusion polypeptides transported out of the cytoplasm without leader sequences
US5563046A (en) * 1993-08-02 1996-10-08 Celtrix Pharmaceuticals, Inc. Fusion polypeptides and proteins
US5527883A (en) 1994-05-06 1996-06-18 Mycogen Corporation Delta-endotoxin expression in pseudomonas fluorescens
EP0797671B1 (en) 1994-12-09 2004-03-03 Novo Nordisk A/S A process of producing extracellular proteins in bacteria
US6495357B1 (en) 1995-07-14 2002-12-17 Novozyme A/S Lipolytic enzymes
IT1283225B1 (it) 1996-03-11 1998-04-16 Renata Maria Anna Ve Cavaliere Ceppi di batteri, composizione farmaceutica contenente uno o piu' di tali ceppi e uso dei medesimi per la prevenzione e la terapia delle
US6361989B1 (en) 1997-10-13 2002-03-26 Novozymes A/S α-amylase and α-amylase variants
US6156552A (en) 1998-02-18 2000-12-05 Novo Nordisk A/S Lipase variants
US20030064435A1 (en) 1998-05-28 2003-04-03 Weiner Joel Hirsch Compositions and methods for protein secretion
US6323007B1 (en) 1998-09-18 2001-11-27 Novozymes A/S 2,6-β-D-fructan hydrolase enzyme and processes for using the enzyme
WO2000024873A1 (en) 1998-10-28 2000-05-04 Genentech, Inc. Process for recovering heterologous polypeptides from bacterial cells
KR100312456B1 (ko) 1999-03-13 2001-11-03 윤덕용 슈도모나스 플루오레슨스 유래의 외래단백질 분비촉진유전자
BR0009393A (pt) 1999-03-29 2002-01-08 Novozymes As Polipeptìdeo, sequência de ácido nucleico isolada, construção de ácido nucleico, vetor de expressão recombinante, célula hospedeira recombinante, método para a produção do polipeptìdeo, uso de um polipeptìdeo, e, composição detergente
US6664386B1 (en) 1999-04-08 2003-12-16 The Research Foundation Of State University Of New York System for efficient secretion of recombinant proteins
US6558939B1 (en) 1999-08-31 2003-05-06 Novozymes, A/S Proteases and variants thereof
DE19944870A1 (de) 1999-09-18 2001-03-29 Aventis Pharma Gmbh Signalsequenzen zur Herstellung von Leu-Hirudin über Sekretion durch E. coli in das Kulturmedium
FI107081B (fi) * 1999-10-19 2001-05-31 Abb Substation Automation Oy Menetelmä ja järjestely osittaispurkauslähteiden lukumäärän selvittämiseksi
US6617143B1 (en) 1999-10-20 2003-09-09 Novozymes A/S Polypeptides having glucanotransferase activity and nucleic acids encoding same
US6509181B1 (en) 2000-04-14 2003-01-21 Novozymes, A/S Polypeptides having haloperoxide activity
US7087412B2 (en) 2000-11-14 2006-08-08 Boehringer Ingelheim International Gmbh Methods for large scale protein production in prokaryotes
GB0027782D0 (en) 2000-11-14 2000-12-27 Boehringer Ingelheim Int Methods fro large scale protein productio in prokaryotes
US7202059B2 (en) 2001-02-20 2007-04-10 Sanofi-Aventis Deutschland Gmbh Fusion proteins capable of being secreted into a fermentation medium
DE10108212A1 (de) 2001-02-20 2002-08-22 Aventis Pharma Gmbh Fusionsprotein zur Sekretion von Wertprotein in bakterielle Überstände
US7419783B2 (en) 2001-11-05 2008-09-02 Research Development Foundation Engineering of leader peptides for the secretion of recombinant proteins in bacteria
AU2002306484A1 (en) 2002-02-13 2003-09-04 Dow Global Technologies Inc. Over-expression of extremozyme genes in pseudomonads and closely related bacteria
WO2003079007A1 (en) 2002-03-15 2003-09-25 The Trustees Of The University Of Pennsylvania Heterologous protein production using the twin arginine translocation pathway
WO2003089455A2 (en) 2002-04-22 2003-10-30 Dow Global Technologies Inc. Low-cost production of peptides
JP2006506049A (ja) 2002-07-03 2006-02-23 ダウ グローバル テクノロジーズ インコーポレイティド ベンゾエート−及びアントラニレート−誘導性プロモーター
AU2004317306B2 (en) 2003-11-21 2010-09-02 Pelican Technology Holdings, Inc. Improved expression systems with Sec-system secretion
AU2005206951B2 (en) 2004-01-16 2010-08-19 Pfenex Inc. Expression of mammalian proteins in Pseudomonas fluorescens
AU2006268780B2 (en) * 2005-07-08 2011-03-24 University Of Zurich Phage display using cotranslational translocation of fusion polypeptides
WO2007142954A2 (en) * 2006-05-30 2007-12-13 Dow Global Technologies Inc. Codon optimization method
EP2468869B1 (en) * 2007-01-31 2015-03-18 Pfenex Inc. Bacterial leader sequences for increased expression
EP2615172A1 (en) * 2007-04-27 2013-07-17 Pfenex Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins

Also Published As

Publication number Publication date
WO2008094986A8 (en) 2009-07-30
JP2018102305A (ja) 2018-07-05
JP2015144608A (ja) 2015-08-13
US7833752B2 (en) 2010-11-16
BRPI0807834A2 (pt) 2014-07-22
EP2108047B1 (en) 2012-10-24
US20100048864A1 (en) 2010-02-25
JP2010517532A (ja) 2010-05-27
WO2008094986A2 (en) 2008-08-07
US7618799B2 (en) 2009-11-17
CA2677179C (en) 2016-02-16
CN102517280A (zh) 2012-06-27
US20110020868A1 (en) 2011-01-27
EP2468869B1 (en) 2015-03-18
US20080193974A1 (en) 2008-08-14
JP6495690B2 (ja) 2019-04-03
EP2108047A2 (en) 2009-10-14
KR101491867B1 (ko) 2015-02-10
JP5714230B2 (ja) 2015-05-07
CN101641441A (zh) 2010-02-03
CA2677179A1 (en) 2008-08-07
EP2468869A1 (en) 2012-06-27
AU2008210538A1 (en) 2008-08-07
KR20090114422A (ko) 2009-11-03
WO2008094986A3 (en) 2008-11-20
AU2008210538B2 (en) 2013-06-06

Similar Documents

Publication Publication Date Title
PL2468869T3 (pl) Bakteryjna sekwencja wiodąca dla zwiększonej ekspresji
GB2448892B (en) Catheter
EP2257678A4 (en) CHANNEL
EP2164407A4 (en) IMPROVED CATHETER
ZA200904015B (en) Toothbrushes
DK200800103A (en) Integreret føringskant for vindturbineblad
PL2136672T3 (pl) Szczoteczka do zębów
EP2182833A4 (en) ENDOSCOPE
AP3369A (en) Function-based habitat design method
PL2318511T3 (pl) Kompozycja bakteryjna
EP2144496A4 (en) IMPROVED AQUACULTURE SYSTEM
GB0801640D0 (en) Toothbrush
TWI365621B (en) Channel estimation
GB0717315D0 (en) Cassette
GB0725147D0 (en) Channel estimation
EP2195319A4 (en) POLYMORPHS OF VALOMACICLOVIR
GB0701694D0 (en) Fishing scope
GB0718966D0 (en) Bacterial vaccine
GB0818317D0 (en) Fishign reel
GB0822725D0 (en) Channel
GB0703853D0 (en) Toothbrush
GB0708137D0 (en) T m
GB0808017D0 (en) shRHA sequences
GB0715878D0 (en) Ward scheme
AU313883S (en) Reel