JP2010207220A5 - - Google Patents
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- JP2010207220A5 JP2010207220A5 JP2010052206A JP2010052206A JP2010207220A5 JP 2010207220 A5 JP2010207220 A5 JP 2010207220A5 JP 2010052206 A JP2010052206 A JP 2010052206A JP 2010052206 A JP2010052206 A JP 2010052206A JP 2010207220 A5 JP2010207220 A5 JP 2010207220A5
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- JP
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- Prior art keywords
- nucleic acid
- target nucleic
- labeled
- oligonucleotides
- moiety
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 108020004707 nucleic acids Proteins 0.000 claims 23
- 150000007523 nucleic acids Chemical class 0.000 claims 23
- 102000039446 nucleic acids Human genes 0.000 claims 23
- 108091034117 Oligonucleotide Proteins 0.000 claims 18
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims 13
- 238000000034 method Methods 0.000 claims 8
- 238000002844 melting Methods 0.000 claims 5
- 230000008018 melting Effects 0.000 claims 5
- 239000011541 reaction mixture Substances 0.000 claims 5
- 239000003153 chemical reaction reagent Substances 0.000 claims 3
- 230000000295 complement effect Effects 0.000 claims 3
- 230000003321 amplification Effects 0.000 claims 2
- 239000007850 fluorescent dye Substances 0.000 claims 2
- 238000003199 nucleic acid amplification method Methods 0.000 claims 2
- 238000011109 contamination Methods 0.000 claims 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US12/400,966 | 2009-03-10 | ||
| US12/400,966 US8039215B2 (en) | 2009-03-10 | 2009-03-10 | Multiplex quantitative nucleic acid amplification and melting assay |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2010207220A JP2010207220A (ja) | 2010-09-24 |
| JP2010207220A5 true JP2010207220A5 (enExample) | 2013-04-25 |
| JP5745774B2 JP5745774B2 (ja) | 2015-07-08 |
Family
ID=42224157
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2010052206A Active JP5745774B2 (ja) | 2009-03-10 | 2010-03-09 | 多重定量的核酸増幅及び融解アッセイ |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US8039215B2 (enExample) |
| EP (1) | EP2228454B1 (enExample) |
| JP (1) | JP5745774B2 (enExample) |
| CN (1) | CN101831496B (enExample) |
| CA (1) | CA2696652C (enExample) |
| ES (1) | ES2589102T3 (enExample) |
Families Citing this family (37)
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| US9169516B2 (en) | 2003-01-24 | 2015-10-27 | University Of Utah Research Foundation | Methods of predicting mortality risk by determining telomere length |
| US9921154B2 (en) | 2011-03-18 | 2018-03-20 | Bio-Rad Laboratories, Inc. | Multiplexed digital assays |
| US8951939B2 (en) * | 2011-07-12 | 2015-02-10 | Bio-Rad Laboratories, Inc. | Digital assays with multiplexed detection of two or more targets in the same optical channel |
| EP2379747B1 (en) * | 2008-12-22 | 2013-07-03 | University of Utah Research Foundation | Monochrome multiplex quantitative pcr |
| US8039215B2 (en) | 2009-03-10 | 2011-10-18 | Roche Molecular Systems, Inc. | Multiplex quantitative nucleic acid amplification and melting assay |
| US8990059B2 (en) * | 2009-10-21 | 2015-03-24 | Koninklijke Philips N.V. | Analyzing tool for amplification reactions |
| JP2014509865A (ja) | 2011-03-18 | 2014-04-24 | バイオ−ラッド・ラボラトリーズ・インコーポレーテッド | シグナルの組合せ使用による多重化デジタルアッセイ |
| JP6059453B2 (ja) * | 2011-05-31 | 2017-01-11 | アークレイ株式会社 | 同一又は近傍の検出波長を有する蛍光色素で修飾された複数のオリゴヌクレオチドを用いて、１種類の波長で複数の遺伝子多型を検出する方法 |
| KR101483576B1 (ko) * | 2011-10-31 | 2015-01-21 | 아크레이 가부시키가이샤 | 유전자 존재량의 측정 방법 |
| JP2014533506A (ja) * | 2011-11-16 | 2014-12-15 | キヤノン ユー．エス． ライフ サイエンシズ， インコーポレイテッドＣａｎｏｎ Ｕ．Ｓ． Ｌｉｆｅ Ｓｃｉｅｎｃｅｓ， Ｉｎｃ． | 高度に相同的なゲノム領域での突然変異の検出 |
| WO2013113748A1 (en) * | 2012-02-02 | 2013-08-08 | Primer Design Ltd | Method for detecting and genotyping target nucleic acid |
| BR112014019168B1 (pt) | 2012-02-03 | 2021-10-05 | California Institute Of Technology | Método capaz de detectar de forma não degenerada presença ou ausência de analitos em um único volume de amostra e método de detecção da presença ou ausência de cada analito dentre uma pluralidade de analitos |
| CN104662172B (zh) | 2012-08-03 | 2018-07-06 | 加州理工学院 | Pcr中具有减少的硬件和要求的多重化和定量 |
| US9970052B2 (en) | 2012-08-23 | 2018-05-15 | Bio-Rad Laboratories, Inc. | Digital assays with a generic reporter |
| CN102952794B (zh) * | 2012-09-05 | 2015-02-25 | 张家港蓝苏生物工程有限公司 | 靶向基因dna分子探针的制备方法 |
| SG11201501550QA (en) * | 2012-09-10 | 2015-03-30 | Biofire Diagnostics Llc | Multiple amplification cycle detection |
| WO2014142575A1 (en) * | 2013-03-13 | 2014-09-18 | Seegene, Inc. | Quantification of target nucleic acid using melting peak analysis |
| CA2912216A1 (en) | 2013-05-22 | 2014-11-27 | Telomere Diagnostics, Inc. | Measures of short telomere abundance |
| WO2015147370A1 (en) * | 2014-03-28 | 2015-10-01 | Seegene, Inc. | Detection of target nucleic acid sequences using different detection temperatures |
| EP3034617A1 (en) | 2014-12-16 | 2016-06-22 | Marcos Isamat Riviere | Method for the simultaneous identification of multiple same-genome fragments or multiple fragments from different genomes using a PCR-technique |
| WO2016108954A1 (en) | 2014-12-30 | 2016-07-07 | Telomere Diagnostics, Inc. | Multiplex quantitative pcr |
| US20180155764A1 (en) * | 2015-06-04 | 2018-06-07 | Mizuho Medy Co., Ltd. | Kit for together detecting multiple target nucleic acids differing from each other and detection method using the same |
| WO2017173035A1 (en) | 2016-04-01 | 2017-10-05 | Chromacode Inc. | Competitive probes for engineering signal generation |
| US10066263B2 (en) | 2016-06-17 | 2018-09-04 | California Institute Of Technology | Nucleic acid reactions and related methods and compositions |
| US11313000B2 (en) | 2016-08-04 | 2022-04-26 | Duke University | Compositions and methods for measuring bacterial growth |
| CN109689889A (zh) * | 2016-09-15 | 2019-04-26 | 豪夫迈·罗氏有限公司 | 用于进行多重pcr的方法 |
| CN106868140B (zh) * | 2017-03-02 | 2022-10-18 | 北京酷搏科技有限公司 | 多重荧光定量pcr的方法 |
| US20190093155A1 (en) * | 2017-05-25 | 2019-03-28 | Roche Molecular Systems, Inc. | Multiplex Nucleic Acid Amplification Assay |
| CN111433372B (zh) * | 2017-10-19 | 2024-01-26 | 生物辐射实验室股份有限公司 | 具有光致发光的非常规和/或反向变化的数字扩增试验 |
| EP3743211A4 (en) * | 2018-01-22 | 2021-11-24 | Luminex Corporation | METHODS AND COMPOSITIONS FOR THE ANALYSIS OF DISCRETE MELT |
| CN112424377A (zh) | 2018-04-17 | 2021-02-26 | 克罗玛科德公司 | 用于多重分析的方法和系统 |
| WO2019203623A1 (en) | 2018-04-20 | 2019-10-24 | Seegene, Inc. . | Method and apparatus for detecting a plurality of target nucleic acid sequences in sample |
| US12203129B2 (en) | 2018-07-03 | 2025-01-21 | ChromaCode, Inc. | Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays |
| US20230068047A1 (en) * | 2021-08-19 | 2023-03-02 | Luminex Corporation | Digital amplification assay analysis method |
| WO2023125552A1 (zh) * | 2021-12-27 | 2023-07-06 | 迈克生物股份有限公司 | 对靶标核酸进行检测的方法 |
| AU2023300374A1 (en) * | 2022-06-29 | 2025-01-16 | Life Technologies Corporation | Compositions, kits, and methods for detecting nucleic acids using intra-channel multiplexing |
| CN117701689A (zh) * | 2022-09-14 | 2024-03-15 | 迈克生物股份有限公司 | 多重pcr反应体系 |
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| EP2116614A1 (en) | 2008-05-06 | 2009-11-11 | Qiagen GmbH | Simultaneous detection of multiple nucleic acid sequences in a reaction |
| US8039215B2 (en) | 2009-03-10 | 2011-10-18 | Roche Molecular Systems, Inc. | Multiplex quantitative nucleic acid amplification and melting assay |
-
2009
- 2009-03-10 US US12/400,966 patent/US8039215B2/en active Active
-
2010
- 2010-03-08 EP EP10002350.6A patent/EP2228454B1/en active Active
- 2010-03-08 ES ES10002350.6T patent/ES2589102T3/es active Active
- 2010-03-09 CA CA2696652A patent/CA2696652C/en active Active
- 2010-03-09 JP JP2010052206A patent/JP5745774B2/ja active Active
- 2010-03-10 CN CN201010163977.7A patent/CN101831496B/zh active Active
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