JP2010207220A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2010207220A5 JP2010207220A5 JP2010052206A JP2010052206A JP2010207220A5 JP 2010207220 A5 JP2010207220 A5 JP 2010207220A5 JP 2010052206 A JP2010052206 A JP 2010052206A JP 2010052206 A JP2010052206 A JP 2010052206A JP 2010207220 A5 JP2010207220 A5 JP 2010207220A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- target nucleic
- labeled
- oligonucleotides
- moiety
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108020004707 nucleic acids Proteins 0.000 claims 23
- 150000007523 nucleic acids Chemical class 0.000 claims 23
- 102000039446 nucleic acids Human genes 0.000 claims 23
- 108091034117 Oligonucleotide Proteins 0.000 claims 18
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims 13
- 238000000034 method Methods 0.000 claims 8
- 238000002844 melting Methods 0.000 claims 5
- 230000008018 melting Effects 0.000 claims 5
- 239000011541 reaction mixture Substances 0.000 claims 5
- 239000003153 chemical reaction reagent Substances 0.000 claims 3
- 230000000295 complement effect Effects 0.000 claims 3
- 230000003321 amplification Effects 0.000 claims 2
- 239000007850 fluorescent dye Substances 0.000 claims 2
- 238000003199 nucleic acid amplification method Methods 0.000 claims 2
- 238000011109 contamination Methods 0.000 claims 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US12/400,966 | 2009-03-10 | ||
| US12/400,966 US8039215B2 (en) | 2009-03-10 | 2009-03-10 | Multiplex quantitative nucleic acid amplification and melting assay |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2010207220A JP2010207220A (ja) | 2010-09-24 |
| JP2010207220A5 true JP2010207220A5 (enExample) | 2013-04-25 |
| JP5745774B2 JP5745774B2 (ja) | 2015-07-08 |
Family
ID=42224157
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2010052206A Active JP5745774B2 (ja) | 2009-03-10 | 2010-03-09 | 多重定量的核酸増幅及び融解アッセイ |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US8039215B2 (enExample) |
| EP (1) | EP2228454B1 (enExample) |
| JP (1) | JP5745774B2 (enExample) |
| CN (1) | CN101831496B (enExample) |
| CA (1) | CA2696652C (enExample) |
| ES (1) | ES2589102T3 (enExample) |
Families Citing this family (38)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004068110A2 (en) | 2003-01-24 | 2004-08-12 | University Of Utah | Methods of predicting mortality risk by determining telomere length |
| US9921154B2 (en) | 2011-03-18 | 2018-03-20 | Bio-Rad Laboratories, Inc. | Multiplexed digital assays |
| US8951939B2 (en) * | 2011-07-12 | 2015-02-10 | Bio-Rad Laboratories, Inc. | Digital assays with multiplexed detection of two or more targets in the same optical channel |
| ES2433718T3 (es) * | 2008-12-22 | 2013-12-12 | University Of Utah Research Foundation | PCR multiplex monocromática cuantitativa |
| US8039215B2 (en) | 2009-03-10 | 2011-10-18 | Roche Molecular Systems, Inc. | Multiplex quantitative nucleic acid amplification and melting assay |
| US8990059B2 (en) * | 2009-10-21 | 2015-03-24 | Koninklijke Philips N.V. | Analyzing tool for amplification reactions |
| CA2830443C (en) * | 2011-03-18 | 2021-11-16 | Bio-Rad Laboratories, Inc. | Multiplexed digital assays with combinatorial use of signals |
| JP6059453B2 (ja) * | 2011-05-31 | 2017-01-11 | アークレイ株式会社 | 同一又は近傍の検出波長を有する蛍光色素で修飾された複数のオリゴヌクレオチドを用いて、１種類の波長で複数の遺伝子多型を検出する方法 |
| EP2774995A4 (en) * | 2011-10-31 | 2015-04-29 | Arkray Inc | METHOD OF MEASURING THE ABUNDANCE OF GENES |
| US20140302501A1 (en) * | 2011-11-16 | 2014-10-09 | Canon U.S. Life Sciences, Inc. | Mutation detection in highly homologous genomic regions |
| WO2013113748A1 (en) * | 2012-02-02 | 2013-08-08 | Primer Design Ltd | Method for detecting and genotyping target nucleic acid |
| EP4361606A3 (en) | 2012-02-03 | 2024-07-10 | California Institute of Technology | Signal encoding and decoding in multiplexed biochemical assays |
| CN108753932B (zh) * | 2012-08-03 | 2022-12-02 | 加州理工学院 | Pcr中具有减少的硬件和要求的多重化和定量 |
| US9970052B2 (en) | 2012-08-23 | 2018-05-15 | Bio-Rad Laboratories, Inc. | Digital assays with a generic reporter |
| CN102952794B (zh) * | 2012-09-05 | 2015-02-25 | 张家港蓝苏生物工程有限公司 | 靶向基因dna分子探针的制备方法 |
| AU2013312191C1 (en) | 2012-09-10 | 2017-10-19 | Biofire Diagnostics, Llc | Multiple amplification cycle detection |
| WO2014142575A1 (en) * | 2013-03-13 | 2014-09-18 | Seegene, Inc. | Quantification of target nucleic acid using melting peak analysis |
| JP6546158B2 (ja) | 2013-05-22 | 2019-07-17 | テロメア ダイアグノスティクス インコーポレイテッド | 短いテロメアの存在量の測定 |
| WO2015147370A1 (en) | 2014-03-28 | 2015-10-01 | Seegene, Inc. | Detection of target nucleic acid sequences using different detection temperatures |
| EP3034617A1 (en) | 2014-12-16 | 2016-06-22 | Marcos Isamat Riviere | Method for the simultaneous identification of multiple same-genome fragments or multiple fragments from different genomes using a PCR-technique |
| WO2016108954A1 (en) | 2014-12-30 | 2016-07-07 | Telomere Diagnostics, Inc. | Multiplex quantitative pcr |
| ES2899826T3 (es) * | 2015-06-04 | 2022-03-14 | Mizuho Medy Co Ltd | Kit para detectar conjuntamente múltiples ácidos nucleicos diana que difieren entre sí y procedimiento de detección usando el mismo |
| EP3436468B1 (en) | 2016-04-01 | 2023-03-22 | Chromacode, Inc. | Competitive probes for engineering signal generation |
| WO2017218777A1 (en) | 2016-06-17 | 2017-12-21 | California Institute Of Technology | Nucleic acid reactions and related methods and compositions |
| WO2018027185A1 (en) | 2016-08-04 | 2018-02-08 | Duke University | Compositions and methods for measuring bacterial growth |
| DK3512959T3 (da) | 2016-09-15 | 2021-10-18 | Hoffmann La Roche | Fremgangsmåder til udførelse af multipleks PCR |
| CN106868140B (zh) * | 2017-03-02 | 2022-10-18 | 北京酷搏科技有限公司 | 多重荧光定量pcr的方法 |
| US20190093155A1 (en) * | 2017-05-25 | 2019-03-28 | Roche Molecular Systems, Inc. | Multiplex Nucleic Acid Amplification Assay |
| CN111433372B (zh) * | 2017-10-19 | 2024-01-26 | 生物辐射实验室股份有限公司 | 具有光致发光的非常规和/或反向变化的数字扩增试验 |
| KR20200115552A (ko) * | 2018-01-22 | 2020-10-07 | 루미넥스 코포레이션 | 별개의 용융 분석을 위한 방법 및 조성물 |
| EP3781710A4 (en) | 2018-04-17 | 2022-01-05 | Chromacode, Inc. | METHODS AND SYSTEMS FOR MULTIPLEX ANALYSIS |
| IL277870B2 (en) | 2018-04-20 | 2025-02-01 | Seegene Inc | Method and device for identifying diverse targets of nucleic acid sequences in a sample |
| US12203129B2 (en) | 2018-07-03 | 2025-01-21 | ChromaCode, Inc. | Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays |
| WO2023023533A1 (en) * | 2021-08-19 | 2023-02-23 | Luminex Corporation | Digital amplification assay analysis method |
| CN116356075A (zh) * | 2021-12-27 | 2023-06-30 | 迈克生物股份有限公司 | 一种用于检测的引物探针、引物探针组及其应用 |
| JP2025501167A (ja) * | 2021-12-27 | 2025-01-17 | マキュラ バイオテクノロジー カンパニー リミテッド | 標的核酸を検出する方法 |
| CN119790162A (zh) * | 2022-06-29 | 2025-04-08 | 生命科技公司 | 用于使用通道内复用检测核酸的组合物、试剂盒和方法 |
| CN117701689A (zh) * | 2022-09-14 | 2024-03-15 | 迈克生物股份有限公司 | 多重pcr反应体系 |
Family Cites Families (33)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4965188A (en) | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
| DE3529478A1 (de) | 1985-08-16 | 1987-02-19 | Boehringer Mannheim Gmbh | 7-desaza-2'desoxyguanosin-nukleotide, verfahren zu deren herstellung und deren verwendung zur nukleinsaeure-sequenzierung |
| US5374553A (en) | 1986-08-22 | 1994-12-20 | Hoffmann-La Roche Inc. | DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima |
| US5795762A (en) | 1986-08-22 | 1998-08-18 | Roche Molecular Systems, Inc. | 5' to 3' exonuclease mutations of thermostable DNA polymerases |
| US5455170A (en) | 1986-08-22 | 1995-10-03 | Hoffmann-La Roche Inc. | Mutated thermostable nucleic acid polymerase enzyme from Thermus species Z05 |
| US5466591A (en) | 1986-08-22 | 1995-11-14 | Hoffmann-La Roche Inc. | 5' to 3' exonuclease mutations of thermostable DNA polymerases |
| US5405774A (en) | 1986-08-22 | 1995-04-11 | Hoffmann-La Roche Inc. | DNA encoding a mutated thermostable nucleic acid polymerase enzyme from thermus species sps17 |
| US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| ATE197718T1 (de) | 1990-09-28 | 2000-12-15 | Hoffmann La Roche | Gereinigte thermostabile nukleinsäurepolymerase aus thermosipho africanus |
| JPH04234422A (ja) | 1990-10-31 | 1992-08-24 | Internatl Business Mach Corp <Ibm> | 二重硬化エポキシバックシール処方物 |
| DE4234086A1 (de) | 1992-02-05 | 1993-08-12 | Diagen Inst Molekularbio | Verfahren zur bestimmung von in vitro amplifizierten nukleinsaeuresequenzen |
| US5538848A (en) | 1994-11-16 | 1996-07-23 | Applied Biosystems Division, Perkin-Elmer Corp. | Method for detecting nucleic acid amplification using self-quenching fluorescence probe |
| GB9513271D0 (en) | 1995-06-29 | 1995-09-06 | Johnson James G | Stress free fish harvester |
| US5773258A (en) | 1995-08-25 | 1998-06-30 | Roche Molecular Systems, Inc. | Nucleic acid amplification using a reversibly inactivated thermostable enzyme |
| DE69739357D1 (de) | 1996-06-04 | 2009-05-28 | Univ Utah Res Found | Überwachung der hybridisierung während pcr |
| US6427156B1 (en) | 1997-01-21 | 2002-07-30 | Xilinx, Inc. | Configurable logic block with AND gate for efficient multiplication in FPGAS |
| US6228628B1 (en) | 1997-07-09 | 2001-05-08 | Roche Molecular Systems | Mutant chimeric DNA polymerase |
| US6140054A (en) * | 1998-09-30 | 2000-10-31 | University Of Utah Research Foundation | Multiplex genotyping using fluorescent hybridization probes |
| US6472156B1 (en) * | 1999-08-30 | 2002-10-29 | The University Of Utah | Homogeneous multiplex hybridization analysis by color and Tm |
| US20040053230A1 (en) * | 2001-03-07 | 2004-03-18 | Schaffer Michael Edward | Real time quantitative pcr with intercalating dye for single and multiplex target dna |
| US7019129B1 (en) * | 2000-05-09 | 2006-03-28 | Biosearch Technologies, Inc. | Dark quenchers for donor-acceptor energy transfer |
| ATE335850T1 (de) | 2001-08-31 | 2006-09-15 | Univ Utah Res Found | Echtzeit-quantifizierung mit internen standards |
| CA2516299A1 (en) * | 2003-02-18 | 2004-09-02 | Applera Corporation | Compositions and methods for multiplex analysis of polynucleotides |
| US20050053950A1 (en) * | 2003-09-08 | 2005-03-10 | Enrique Zudaire Ubani | Protocol and software for multiplex real-time PCR quantification based on the different melting temperatures of amplicons |
| WO2006040187A2 (en) * | 2004-10-11 | 2006-04-20 | Epigenomics Ag | A method for the carry-over protection in dna amplification systems targeting methylation analysis achieved by a modified pre-treatment of nucleic acids |
| WO2006044994A2 (en) * | 2004-10-18 | 2006-04-27 | Brandeis University | Primers, probes and methods for nucleic acid amplification |
| US7465561B2 (en) | 2005-06-30 | 2008-12-16 | Roche Molecular Systems, Inc. | Probes and methods for hepatitis C virus typing using single probe analysis |
| JP4815980B2 (ja) * | 2005-10-05 | 2011-11-16 | 株式会社島津製作所 | 融解曲線測定による遺伝子増幅産物の解析方法 |
| US20100285468A1 (en) | 2007-09-24 | 2010-11-11 | Allelogic Biosciences Corporation | Detection and/or quantification of nucleic acids |
| EP2336358A1 (en) | 2008-05-06 | 2011-06-22 | QIAGEN GmbH | Simultaneous detection of multiple nucleic acid sequences in a reaction |
| US8039215B2 (en) | 2009-03-10 | 2011-10-18 | Roche Molecular Systems, Inc. | Multiplex quantitative nucleic acid amplification and melting assay |
-
2009
- 2009-03-10 US US12/400,966 patent/US8039215B2/en active Active
-
2010
- 2010-03-08 EP EP10002350.6A patent/EP2228454B1/en active Active
- 2010-03-08 ES ES10002350.6T patent/ES2589102T3/es active Active
- 2010-03-09 JP JP2010052206A patent/JP5745774B2/ja active Active
- 2010-03-09 CA CA2696652A patent/CA2696652C/en active Active
- 2010-03-10 CN CN201010163977.7A patent/CN101831496B/zh active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Kim et al. | Loop-mediated isothermal amplification-based nucleic acid lateral flow assay for the specific and multiplex detection of genetic markers | |
| CN104271767B (zh) | 多路生化测定中信号的编码和解码 | |
| JP7477183B2 (ja) | 新規な等温単一反応用プローブセット及びその用途 | |
| CA2696652A1 (en) | Multiplex quantitative nucleic acid amplification and melting assay | |
| Yue et al. | Programmable strand displacement-based magnetic separation for simultaneous amplified detection of multiplex microRNAs by chemiluminescence imaging array | |
| JP2019522463A5 (enExample) | ||
| JP6431076B2 (ja) | リアルタイムｐｃｒ信号におけるジャンプの検出及び補正 | |
| Sapountzi et al. | Lateral flow devices for nucleic acid analysis exploiting quantum dots as reporters | |
| Bi et al. | Label-free chemiluminescent aptasensor for platelet-derived growth factor detection based on exonuclease-assisted cascade autocatalytic recycling amplification | |
| WO2007115242A3 (en) | Binary probes for fluorescent analysis of nucleic acids | |
| TW201530119A (zh) | 分析試驗裝置、套組及使用方法 | |
| Dong et al. | A signal-flexible gene diagnostic strategy coupling loop-mediated isothermal amplification with hybridization chain reaction | |
| Zhu et al. | Label‐Free Detection of MicroRNA: Two‐Step Signal Enhancement with a Hairpin‐Probe‐Based Graphene Fluorescence Switch and Isothermal Amplification | |
| CN104419765A (zh) | 核酸序列测定方法及装置、核酸序列测定用元件及其制造方法 | |
| JP2011530296A5 (enExample) | ||
| CN116083543A (zh) | 用于工程化信号产生的竞争性探针 | |
| Miao et al. | Terminal deoxynucleotidyl transferase-induced DNAzyme nanowire sensor for colorimetric detection of lipopolysaccharides | |
| JP2021003050A5 (enExample) | ||
| WO2007087336A3 (en) | Methods for detecting nucleic acids using multiple signals | |
| JP2019536441A5 (enExample) | ||
| Wang et al. | Colorimetric biosensor based on a DNAzyme primer and its application in logic gate operations for DNA screening | |
| Zhuang et al. | Research progress of loop-mediated isothermal amplification in the detection of Salmonella for food safety applications | |
| JP2020114225A5 (enExample) | ||
| JP2018503390A (ja) | 基質分子 | |
| JP2014533502A5 (enExample) |