CN101831496B - 多重定量核酸扩增及解链测定法 - Google Patents
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
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| US9921154B2 (en) | 2011-03-18 | 2018-03-20 | Bio-Rad Laboratories, Inc. | Multiplexed digital assays |
| CA2748265C (en) * | 2008-12-22 | 2018-04-03 | University Of Utah Research Foundation | Monochrome multiplex quantitative pcr |
| US8039215B2 (en) | 2009-03-10 | 2011-10-18 | Roche Molecular Systems, Inc. | Multiplex quantitative nucleic acid amplification and melting assay |
| CN102576389B (zh) * | 2009-10-21 | 2016-08-03 | 皇家飞利浦电子股份有限公司 | 用于扩增反应的分析工具 |
| CN103534360A (zh) * | 2011-03-18 | 2014-01-22 | 伯乐生命医学产品有限公司 | 借助对信号的组合使用进行的多重数字分析 |
| JP6059453B2 (ja) * | 2011-05-31 | 2017-01-11 | アークレイ株式会社 | 同一又は近傍の検出波長を有する蛍光色素で修飾された複数のオリゴヌクレオチドを用いて、１種類の波長で複数の遺伝子多型を検出する方法 |
| EP2774995A4 (en) * | 2011-10-31 | 2015-04-29 | Arkray Inc | METHOD OF MEASURING THE ABUNDANCE OF GENES |
| US20140302501A1 (en) * | 2011-11-16 | 2014-10-09 | Canon U.S. Life Sciences, Inc. | Mutation detection in highly homologous genomic regions |
| WO2013113748A1 (en) * | 2012-02-02 | 2013-08-08 | Primer Design Ltd | Method for detecting and genotyping target nucleic acid |
| EP4361607A3 (en) | 2012-02-03 | 2024-07-17 | California Institute of Technology | Signal encoding and decoding in multiplexed biochemical assays |
| EP2880184B1 (en) * | 2012-08-03 | 2021-03-31 | California Institute of Technology | Multiplexing and quantification in pcr with reduced hardware and requirements |
| US9970052B2 (en) | 2012-08-23 | 2018-05-15 | Bio-Rad Laboratories, Inc. | Digital assays with a generic reporter |
| CN102952794B (zh) * | 2012-09-05 | 2015-02-25 | 张家港蓝苏生物工程有限公司 | 靶向基因dna分子探针的制备方法 |
| EP2893062A4 (en) | 2012-09-10 | 2016-08-17 | Biofire Diagnostics Llc | DETECTION OF MULTIPLE REINFORCEMENT CYCLES |
| KR101943623B1 (ko) * | 2013-03-13 | 2019-01-30 | 주식회사 씨젠 | 멜팅 피크 분석을 이용한 타겟 핵산서열의 정량 |
| EP2999800B1 (en) | 2013-05-22 | 2019-09-25 | Telomere Diagnostics Inc. | Measures of short telomere abundance |
| WO2015147370A1 (en) | 2014-03-28 | 2015-10-01 | Seegene, Inc. | Detection of target nucleic acid sequences using different detection temperatures |
| EP3034617A1 (en) | 2014-12-16 | 2016-06-22 | Marcos Isamat Riviere | Method for the simultaneous identification of multiple same-genome fragments or multiple fragments from different genomes using a PCR-technique |
| CA2971169A1 (en) | 2014-12-30 | 2016-07-07 | Telomere Diagnostics, Inc. | Multiplex quantitative pcr |
| MX2017015673A (es) * | 2015-06-04 | 2018-06-19 | Mizuho Medy Co Ltd | Kit para detectar conjuntamente multiples acidos nucleicos con especificidad de objetivo que difieren entre si y metodo de deteccion utilizando el mismo. |
| WO2017173035A1 (en) | 2016-04-01 | 2017-10-05 | Chromacode Inc. | Competitive probes for engineering signal generation |
| WO2017218777A1 (en) * | 2016-06-17 | 2017-12-21 | California Institute Of Technology | Nucleic acid reactions and related methods and compositions |
| WO2018027185A1 (en) * | 2016-08-04 | 2018-02-08 | Duke University | Compositions and methods for measuring bacterial growth |
| JP7063886B2 (ja) | 2016-09-15 | 2022-05-09 | エフ．ホフマン－ラ ロシュ アーゲー | マルチプレックスリアルタイムｐｃｒを実施する方法 |
| CN106868140B (zh) * | 2017-03-02 | 2022-10-18 | 北京酷搏科技有限公司 | 多重荧光定量pcr的方法 |
| US20190093155A1 (en) * | 2017-05-25 | 2019-03-28 | Roche Molecular Systems, Inc. | Multiplex Nucleic Acid Amplification Assay |
| CN111433372B (zh) * | 2017-10-19 | 2024-01-26 | 生物辐射实验室股份有限公司 | 具有光致发光的非常规和/或反向变化的数字扩增试验 |
| CN111629831A (zh) * | 2018-01-22 | 2020-09-04 | 卢米耐克斯公司 | 用于离散解链分析的方法和组合物 |
| EP3781710A4 (en) | 2018-04-17 | 2022-01-05 | Chromacode, Inc. | METHODS AND SYSTEMS FOR MULTIPLEX ANALYSIS |
| AU2019255933B2 (en) | 2018-04-20 | 2022-08-25 | Seegene, Inc. . | Method and apparatus for detecting a plurality of target nucleic acid sequences in sample |
| US12203129B2 (en) | 2018-07-03 | 2025-01-21 | ChromaCode, Inc. | Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays |
| EP4388132A1 (en) * | 2021-08-19 | 2024-06-26 | Luminex Corporation | Digital amplification assay analysis method |
| US20250066857A1 (en) * | 2021-12-27 | 2025-02-27 | Maccura Biotechnology Co., Ltd. | Method for detecting target nucleic acid |
| EP4547861A1 (en) * | 2022-06-29 | 2025-05-07 | Life Technologies Corporation | Compositions, kits, and methods for detecting nucleic acids using intra-channel multiplexing |
| CN117701689A (zh) * | 2022-09-14 | 2024-03-15 | 迈克生物股份有限公司 | 多重pcr反应体系 |
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| Real-Time Multiplex PCR Assays;Carl T. Wittwer et al.;《METHODS》;20011231;第25卷;430-442 * |
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| CA2696652A1 (en) | 2010-09-10 |
| CN101831496A (zh) | 2010-09-15 |
| EP2228454B1 (en) | 2016-06-22 |
| US20100233686A1 (en) | 2010-09-16 |
| JP2010207220A (ja) | 2010-09-24 |
| US8039215B2 (en) | 2011-10-18 |
| JP5745774B2 (ja) | 2015-07-08 |
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