CA2696652C - Multiplex quantitative nucleic acid amplification and melting assay - Google Patents

Multiplex quantitative nucleic acid amplification and melting assay Download PDF

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Publication number
CA2696652C
CA2696652C CA2696652A CA2696652A CA2696652C CA 2696652 C CA2696652 C CA 2696652C CA 2696652 A CA2696652 A CA 2696652A CA 2696652 A CA2696652 A CA 2696652A CA 2696652 C CA2696652 C CA 2696652C
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Prior art keywords
nucleic acid
target nucleic
melting
labeled
amplification
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CA2696652A
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English (en)
French (fr)
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CA2696652A1 (en
Inventor
Russell Higuchi
Cherie Holcomb
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F Hoffmann La Roche AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CA2696652A 2009-03-10 2010-03-09 Multiplex quantitative nucleic acid amplification and melting assay Active CA2696652C (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US12/400,966 US8039215B2 (en) 2009-03-10 2009-03-10 Multiplex quantitative nucleic acid amplification and melting assay
US12/400,966 2009-03-10

Publications (2)

Publication Number Publication Date
CA2696652A1 CA2696652A1 (en) 2010-09-10
CA2696652C true CA2696652C (en) 2015-09-08

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Family Applications (1)

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CA2696652A Active CA2696652C (en) 2009-03-10 2010-03-09 Multiplex quantitative nucleic acid amplification and melting assay

Country Status (6)

Country Link
US (1) US8039215B2 (enExample)
EP (1) EP2228454B1 (enExample)
JP (1) JP5745774B2 (enExample)
CN (1) CN101831496B (enExample)
CA (1) CA2696652C (enExample)
ES (1) ES2589102T3 (enExample)

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KR102468633B1 (ko) * 2016-09-15 2022-11-18 에프. 호프만-라 로슈 아게 멀티플렉스 실시간 pcr 수행 방법
CN106868140B (zh) * 2017-03-02 2022-10-18 北京酷搏科技有限公司 多重荧光定量pcr的方法
US20190093155A1 (en) * 2017-05-25 2019-03-28 Roche Molecular Systems, Inc. Multiplex Nucleic Acid Amplification Assay
US11072820B2 (en) 2017-10-19 2021-07-27 Bio-Rad Laboratories, Inc. Digital amplification assays with unconventional and/or inverse changes in photoluminescence
EP3743211A4 (en) * 2018-01-22 2021-11-24 Luminex Corporation METHODS AND COMPOSITIONS FOR THE ANALYSIS OF DISCRETE MELT
CN112424377A (zh) 2018-04-17 2021-02-26 克罗玛科德公司 用于多重分析的方法和系统
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WO2023023533A1 (en) * 2021-08-19 2023-02-23 Luminex Corporation Digital amplification assay analysis method
EP4458984A4 (en) * 2021-12-27 2026-05-13 Maccura Biotechnology Co Ltd TARGET NUCLEIC ACID DETECTION METHOD
CN116356075A (zh) * 2021-12-27 2023-06-30 迈克生物股份有限公司 一种用于检测的引物探针、引物探针组及其应用
CN119768474A (zh) * 2022-06-29 2025-04-04 生命科技股份有限公司 多重染料化合物
CN117701689A (zh) * 2022-09-14 2024-03-15 迈克生物股份有限公司 多重pcr反应体系

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Also Published As

Publication number Publication date
CN101831496B (zh) 2017-04-12
EP2228454A1 (en) 2010-09-15
JP5745774B2 (ja) 2015-07-08
US20100233686A1 (en) 2010-09-16
CA2696652A1 (en) 2010-09-10
US8039215B2 (en) 2011-10-18
JP2010207220A (ja) 2010-09-24
EP2228454B1 (en) 2016-06-22
CN101831496A (zh) 2010-09-15
ES2589102T3 (es) 2016-11-10

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