ATE335850T1 - Echtzeit-quantifizierung mit internen standards - Google Patents

Echtzeit-quantifizierung mit internen standards

Info

Publication number
ATE335850T1
ATE335850T1 AT02256006T AT02256006T ATE335850T1 AT E335850 T1 ATE335850 T1 AT E335850T1 AT 02256006 T AT02256006 T AT 02256006T AT 02256006 T AT02256006 T AT 02256006T AT E335850 T1 ATE335850 T1 AT E335850T1
Authority
AT
Austria
Prior art keywords
nucleic acid
mass fraction
real
pcr
internal standards
Prior art date
Application number
AT02256006T
Other languages
English (en)
Inventor
David J Eyre
Randy P Rasmussen
Brian E Caplin
Wade R Stevenson
Deepika Marine Desilva
Original Assignee
Univ Utah Res Found
Idaho Technology Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Utah Res Found, Idaho Technology Inc filed Critical Univ Utah Res Found
Application granted granted Critical
Publication of ATE335850T1 publication Critical patent/ATE335850T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/708—Specific hybridization probes for papilloma

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
AT02256006T 2001-08-31 2002-08-29 Echtzeit-quantifizierung mit internen standards ATE335850T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US31661401P 2001-08-31 2001-08-31

Publications (1)

Publication Number Publication Date
ATE335850T1 true ATE335850T1 (de) 2006-09-15

Family

ID=23229815

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02256006T ATE335850T1 (de) 2001-08-31 2002-08-29 Echtzeit-quantifizierung mit internen standards

Country Status (7)

Country Link
US (1) US7630837B2 (de)
EP (1) EP1288314B1 (de)
JP (1) JP4814479B2 (de)
AT (1) ATE335850T1 (de)
CA (1) CA2400601A1 (de)
DE (1) DE60213730T2 (de)
ES (1) ES2269614T3 (de)

Families Citing this family (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7373253B2 (en) 2002-02-12 2008-05-13 Idaho Technology Multi-test analysis of real-time nucleic acid amplification
ATE384139T1 (de) * 2002-02-12 2008-02-15 Univ Utah Res Found Multi-test-analyse von echzeit- nukleinsäureamplifikationen
US20070128608A1 (en) * 2003-12-19 2007-06-07 Kankyo Engineering Co., Ltd. Novel mixtures for assaying nucleic acid, novel method of assaying nucleic acid with the use of the same and nucleic acid probe to be used therefor
US20050277121A1 (en) * 2004-06-11 2005-12-15 Ambion, Inc. Crude biological derivatives competent for nucleic acid detection
EP1612283B1 (de) * 2004-06-30 2011-10-19 Universiteit Gent Genotyp spezifischer Detektion von Chlamydophila psittaci
WO2006137830A2 (en) * 2004-08-09 2006-12-28 Marshfield Clinic Research Foundation Detection of mycobacterium
KR100738073B1 (ko) 2004-09-01 2007-07-12 삼성전자주식회사 실시간 핵산 증폭 데이터로부터 초기 핵산 농도를정량화하는 방법
JP4777631B2 (ja) * 2004-09-27 2011-09-21 株式会社日立ハイテクノロジーズ 核酸増幅分析法および装置
EP2348320B1 (de) * 2005-03-10 2024-05-01 Gen-Probe Incorporated Verfahren und Systeme zur Erkennung mehrerer Fluoreszenzemissionssignale
WO2006099255A2 (en) 2005-03-10 2006-09-21 Gen-Probe Incorporated Systems and methods to perform assays for detecting or quantifying analytes within samples
GB2424886A (en) * 2005-04-04 2006-10-11 Dxs Ltd Polynucleotide primers against epidermal growth factor receptor and method of detecting gene mutations
CA2610836A1 (en) * 2005-06-15 2006-12-28 Gen-Probe Incorporated Methods for quantitative analysis of a nucleic acid amplification reaction
RU2005132940A (ru) * 2006-03-01 2007-11-27 Институт биохимии и генетики Уфимского научного центра РАН (RU) Способ детекции специфических фрагментов днк или рнк с помощью полимеразной цепной реакции в режиме реального времени
EP1955241B1 (de) * 2005-11-14 2011-03-30 Gen-Probe Incorporated Parametrisches kalibrationsverfahren
US20070148639A1 (en) * 2005-12-02 2007-06-28 Chagovetz Alexander M Methods and devices for nucleic acid amplification on a surface
US20070148677A1 (en) * 2005-12-02 2007-06-28 Chagovetz Alexander M Methods and systems for acquiring real-time quantitative melt data
WO2008070328A2 (en) * 2006-10-24 2008-06-12 Celera Corporation Systems and methods for baselining and real-time pcr data analysis
WO2008057375A2 (en) * 2006-11-03 2008-05-15 Siemens Healthcare Diagnostics Inc. Random access system and method for polymerase chain reaction testing
WO2008067567A2 (en) * 2006-11-30 2008-06-05 Gen-Probe Incorporated Quantitative method employing adjustment of pre-defined master calibration curves
US20100285468A1 (en) * 2007-09-24 2010-11-11 Allelogic Biosciences Corporation Detection and/or quantification of nucleic acids
US20090181391A1 (en) * 2007-11-01 2009-07-16 Zymo Research Corporation Methods for analysis of dna methylation percentage
JP5542439B2 (ja) * 2007-12-26 2014-07-09 アークレイ株式会社 核酸増幅判定方法および核酸増幅判定装置
US9518288B2 (en) 2008-04-11 2016-12-13 University Of Utah Research Foundation Methods and compositions related to quantitative, array based methylation analysis
WO2010010951A1 (ja) * 2008-07-24 2010-01-28 Kuroda Masahiko 子宮内膜症関連疾患の指標ｍＲＮＡの発現の検出方法およびそれに用いるプローブ
EP4008795A1 (de) 2008-08-12 2022-06-08 Stokes Bio Limited Verfahren für die digitale pcr
EP2166470A1 (de) * 2008-09-19 2010-03-24 Corbett Research Pty Ltd Analyse von Schmelzkurven, insbesondere dsDNA- und Proteinschmelzkurven
EP2356245B1 (de) 2008-11-07 2018-12-26 University of Utah Research Foundation Allelamplifikationstendenz
US8346485B2 (en) 2008-11-25 2013-01-01 Quest Diagnostics Investments Incorporated Methods and apparatuses for estimating initial target nucleic acid concentration in a sample by modeling background signal and cycle-dependent amplification efficiency of a polymerase chain reaction
EP2391458A4 (de) * 2009-01-30 2017-10-18 Unified Brands, Inc. Besteck- und geschirr-teilewaschmaschine und verfahren dafür
US8606527B2 (en) * 2009-02-27 2013-12-10 Bio-Rad Laboratories, Inc. SNP detection by melt curve clustering
US8039215B2 (en) * 2009-03-10 2011-10-18 Roche Molecular Systems, Inc. Multiplex quantitative nucleic acid amplification and melting assay
WO2010119407A1 (en) * 2009-04-16 2010-10-21 Koninklijke Philips Electronics N.V. Methods for nucleic acid quantification
WO2012097303A1 (en) * 2011-01-14 2012-07-19 Brandeis University Single tube quantitative polymerase chain reaction (pcr)
GB2506375B (en) 2012-09-27 2017-10-18 Epistem Ltd Data processing and analysis systems
WO2014142575A1 (en) * 2013-03-13 2014-09-18 Seegene, Inc. Quantification of target nucleic acid using melting peak analysis
ES2795677T3 (es) 2013-03-15 2020-11-24 Gen Probe Inc Método, aparato y producto de programa informático para calibración
EP2992113B1 (de) * 2013-05-03 2019-08-28 Seegene, Inc. Quantifizierung von zielnukleinsäuren mittels gemeinsamer interner kontrolle
WO2016160823A1 (en) * 2015-04-03 2016-10-06 Becton, Dickinson And Company Methods of amplifying nucleic acids and compositions and kits for practicing the same
EP3130679B1 (de) * 2015-08-13 2018-02-28 Cladiac GmbH Verfahren und testsystem zum nachweis und/oder quantifizieren einer ziel-nukleinsäure in einer probe
US11332781B2 (en) 2016-06-23 2022-05-17 Canon U.S.A., Inc. Fitting melting curve data to determine copy number variation
CN114214395A (zh) * 2021-12-31 2022-03-22 上海星耀医学科技发展有限公司 一种提高基因检测准确性的核酸扩增方法与试剂盒

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5118801A (en) * 1988-09-30 1992-06-02 The Public Health Research Institute Nucleic acid process containing improved molecular switch
US5219727A (en) * 1989-08-21 1993-06-15 Hoffmann-Laroche Inc. Quantitation of nucleic acids using the polymerase chain reaction
US5455175A (en) * 1990-06-04 1995-10-03 University Of Utah Research Foundation Rapid thermal cycling device
US5817462A (en) * 1995-02-21 1998-10-06 Applied Spectral Imaging Method for simultaneous detection of multiple fluorophores for in situ hybridization and multicolor chromosome painting and banding
US5798262A (en) * 1991-02-22 1998-08-25 Applied Spectral Imaging Ltd. Method for chromosomes classification
DE4137934A1 (de) 1991-11-18 1993-05-19 Boehringer Mannheim Gmbh Neue pentacyklische verbindungen und ihre verwendung als absorptions- oder fluoreszenzfarbstoffe
US5224775C2 (en) * 1992-03-02 2002-04-23 Ta Instr Inc Method and apparatus for modulated differential analysis
US5273632A (en) * 1992-11-19 1993-12-28 University Of Utah Research Foundation Methods and apparatus for analysis of chromatographic migration patterns
US5436149A (en) * 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
US5912165A (en) * 1993-08-18 1999-06-15 Applied Spectral Imaging Ltd Method for chromosome classification by decorrelaiton statistical analysis and hardware therefore
US5580728A (en) * 1994-06-17 1996-12-03 Perlin; Mark W. Method and system for genotyping
US5748491A (en) * 1995-12-20 1998-05-05 The Perkin-Elmer Corporation Deconvolution method for the analysis of data resulting from analytical separation processes
US6174670B1 (en) * 1996-06-04 2001-01-16 University Of Utah Research Foundation Monitoring amplification of DNA during PCR
GB2333597B (en) * 1996-10-29 2001-04-18 Univ Nebraska At Lincoln Method for detecting point mutations in dna utilizing fluorescence energy transfer
US5985120A (en) * 1997-06-12 1999-11-16 University Of Massachusetts Rapid analysis of analyte solutions
US6268131B1 (en) * 1997-12-15 2001-07-31 Sequenom, Inc. Mass spectrometric methods for sequencing nucleic acids
US6387621B1 (en) * 1999-04-27 2002-05-14 University Of Utah Research Foundation Automated analysis of real-time nucleic acid amplification
US6221600B1 (en) * 1999-10-08 2001-04-24 Board Of Regents, The University Of Texas System Combinatorial oligonucleotide PCR: a method for rapid, global expression analysis

Also Published As

Publication number Publication date
DE60213730D1 (de) 2006-09-21
JP2003180378A (ja) 2003-07-02
JP4814479B2 (ja) 2011-11-16
US7630837B2 (en) 2009-12-08
CA2400601A1 (en) 2003-02-28
US20030104438A1 (en) 2003-06-05
EP1288314B1 (de) 2006-08-09
EP1288314A3 (de) 2004-02-25
DE60213730T2 (de) 2007-08-16
ES2269614T3 (es) 2007-04-01
EP1288314A2 (de) 2003-03-05

Similar Documents

Publication Publication Date Title
DE60213730D1 (de) Echtzeit-Quantifizierung mit internen Standards
DE68908900D1 (de) Nachweis und bestimmung der menge an rns und dns.
DK0497784T5 (da) Kvantificering af nukleinsyrer under anvendelse af polymerasekædereaktion
ATE455870T1 (de) Oligonukleotide zur schnellen bestimmung von mikrobieller dns/rns
ATE230801T1 (de) Verfahren zur detektion von zielnukleinsäure, verfahren zu ihrer quantifizierung und pyrylium- verbindungen zur chemilumineszenz-analyse
DE69820111D1 (de) Massenspektroskopische verfahren zur sequenzierung von nukleinsäuren
ATE362995T1 (de) Verfahren zum nachweis von cytosinmethylierungsmustern mit hoher sensitivität
ATE322558T1 (de) Sensible und multiplexe diagnostische tests zur proteinanalyse
ATE267878T1 (de) Die bestimmung von nukleinsäuremutationen durch analyse des sputum
ATE522812T1 (de) Verfahren und zusammensetzung zum bestimmen von homocystein
SE0301951D0 (sv) New method
ATE458201T1 (de) Methode zur beurteilung von rheumatoider arthritis durch bestimmung von anti-ccp und interleukin 6
ATE298004T1 (de) Diagnostische sequenzierung durch eine kombination von spezifischer spaltung und massenspektrometrie
ATE115728T1 (de) Verfahren zur verminderung von störungen in einer fluoreszenzbestimmung.
HUP0204549A2 (hu) Eljárás adenovírus kimutatására és mennyiségi meghatározására
DE60310012D1 (de) Verfahren, Assay und Kit zur Quantifizierung von HIV-Proteasehemmer
DE60228952D1 (de) Nachweis von Legionella durch ein PCR und FRET verwendendes Verfahren
ATE384141T1 (de) Methode zur quantitativen bestimmung von polynukleotiden in einem gemisch
ATE330037T1 (de) Verfahren zur altersbestimmung von individuen
DE60226969D1 (de) Analytische methode und testkit
ATE315107T1 (de) Verfahren und kit zur früherkennung von krebs
DE602004031571D1 (de) Enzymhemmungsimmunverfahren
DE60121547D1 (de) Electrochemisches verfahren zum nachweis von nukleinsäuren
JP2000125900A5 (de)
DE60012723D1 (de) Verfahren zur bestimmung von disaccharidasen

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties