JP2017506914A5 - - Google Patents
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- JP2017506914A5 JP2017506914A5 JP2016570186A JP2016570186A JP2017506914A5 JP 2017506914 A5 JP2017506914 A5 JP 2017506914A5 JP 2016570186 A JP2016570186 A JP 2016570186A JP 2016570186 A JP2016570186 A JP 2016570186A JP 2017506914 A5 JP2017506914 A5 JP 2017506914A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- compound
- reporter
- amount
- complex
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 108020004707 nucleic acids Proteins 0.000 claims 45
- 150000007523 nucleic acids Chemical class 0.000 claims 45
- 102000039446 nucleic acids Human genes 0.000 claims 45
- 150000001875 compounds Chemical class 0.000 claims 41
- 238000000034 method Methods 0.000 claims 19
- 230000003321 amplification Effects 0.000 claims 12
- 238000003199 nucleic acid amplification method Methods 0.000 claims 12
- 238000002866 fluorescence resonance energy transfer Methods 0.000 claims 8
- 238000006243 chemical reaction Methods 0.000 claims 6
- 230000008878 coupling Effects 0.000 claims 3
- 238000010168 coupling process Methods 0.000 claims 3
- 238000005859 coupling reaction Methods 0.000 claims 3
- 108091034117 Oligonucleotide Proteins 0.000 claims 2
- 238000000137 annealing Methods 0.000 claims 2
- 125000004122 cyclic group Chemical group 0.000 claims 2
- 238000011901 isothermal amplification Methods 0.000 claims 2
- 230000000737 periodic effect Effects 0.000 claims 2
- 108060002716 Exonuclease Proteins 0.000 claims 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims 1
- 239000012491 analyte Substances 0.000 claims 1
- 230000015572 biosynthetic process Effects 0.000 claims 1
- 238000011088 calibration curve Methods 0.000 claims 1
- 230000009918 complex formation Effects 0.000 claims 1
- 230000006835 compression Effects 0.000 claims 1
- 238000007906 compression Methods 0.000 claims 1
- 230000000694 effects Effects 0.000 claims 1
- 102000013165 exonuclease Human genes 0.000 claims 1
- 230000003993 interaction Effects 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB1403076.1 | 2014-02-21 | ||
| GBGB1403076.1A GB201403076D0 (en) | 2014-02-21 | 2014-02-21 | Methods for detecting multiple nucleic acids in a sample |
| PCT/EP2015/053759 WO2015124779A1 (en) | 2014-02-21 | 2015-02-23 | Methods for detecting multiple nucleic acids in a sample |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020046081A Division JP2020114225A (ja) | 2014-02-21 | 2020-03-17 | サンプル中の複数の核酸を検出するための方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2017506914A JP2017506914A (ja) | 2017-03-16 |
| JP2017506914A5 true JP2017506914A5 (enExample) | 2018-04-05 |
| JP6727137B2 JP6727137B2 (ja) | 2020-07-22 |
Family
ID=50482582
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016570186A Active JP6727137B2 (ja) | 2014-02-21 | 2015-02-23 | サンプル中の複数の核酸を検出するための方法 |
| JP2020046081A Pending JP2020114225A (ja) | 2014-02-21 | 2020-03-17 | サンプル中の複数の核酸を検出するための方法 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020046081A Pending JP2020114225A (ja) | 2014-02-21 | 2020-03-17 | サンプル中の複数の核酸を検出するための方法 |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US10246737B2 (enExample) |
| EP (3) | EP3800269B1 (enExample) |
| JP (2) | JP6727137B2 (enExample) |
| CN (1) | CN106687598B (enExample) |
| CA (1) | CA2937832C (enExample) |
| GB (1) | GB201403076D0 (enExample) |
| HK (1) | HK1231512A1 (enExample) |
| WO (1) | WO2015124779A1 (enExample) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB201403076D0 (en) | 2014-02-21 | 2014-04-09 | ALERE TECHNOLOGIES GmbH | Methods for detecting multiple nucleic acids in a sample |
| GB201713251D0 (en) * | 2017-08-18 | 2017-10-04 | Lgc Genomics Ltd | Methods and kits for detection of nucleic acid molecules |
| CN110672569A (zh) * | 2019-09-30 | 2020-01-10 | 中国科学院宁波工业技术研究院慈溪生物医学工程研究所 | 一种dna或rna检测体系、检测方法和它们的应用 |
| CN112553376B (zh) * | 2020-12-23 | 2023-06-02 | 四川大学 | 引物组、试剂盒、区分不同ibv疫苗毒株的多重pcr检测方法及其应用 |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5538848A (en) * | 1994-11-16 | 1996-07-23 | Applied Biosystems Division, Perkin-Elmer Corp. | Method for detecting nucleic acid amplification using self-quenching fluorescence probe |
| CA2379125C (en) | 1999-07-02 | 2009-04-07 | Clondiag Chip Technologies Gmbh | Microchip matrix device for duplicating and characterizing nucleic acids |
| US6312929B1 (en) * | 2000-12-22 | 2001-11-06 | Cepheid | Compositions and methods enabling a totally internally controlled amplification reaction |
| CA2436518A1 (en) * | 2001-01-30 | 2002-08-08 | Vertex Pharmaceuticals Incorporated | A quantitative assay for nucleic acids |
| AU2002365212A1 (en) * | 2001-07-15 | 2003-09-02 | Keck Graduate Institute | Gene expression analysis using nicking agents |
| US7226732B2 (en) * | 2001-07-16 | 2007-06-05 | Cepheid | Methods, apparatus, and computer programs for verifying the integrity of a probe |
| EP1493822A1 (de) * | 2003-07-02 | 2005-01-05 | Labor Becker, Olgemoeller & Kollegen GbR | Detektionsverfahren für Nucleinsäuren mit interner Amplifikationskontrolle |
| DE102004022263A1 (de) | 2004-05-06 | 2005-12-15 | Clondiag Chip Technologies Gmbh | Vorrichtung und Verfahren zum Nachweis von molekularen Wechselwirkungen |
| WO2008055915A2 (en) | 2006-11-06 | 2008-05-15 | Clondiag Gmbh | Device and process for assays using binding members |
| CN102851369B (zh) * | 2006-12-13 | 2015-01-21 | 卢米耐克斯公司 | 用于实时pcr多重分析的系统和方法 |
| CA2946024C (en) | 2007-05-03 | 2020-06-02 | Clondiag Gmbh | Devices and methods for detecting analytes |
| GB201403076D0 (en) | 2014-02-21 | 2014-04-09 | ALERE TECHNOLOGIES GmbH | Methods for detecting multiple nucleic acids in a sample |
-
2014
- 2014-02-21 GB GBGB1403076.1A patent/GB201403076D0/en not_active Ceased
-
2015
- 2015-02-23 EP EP20204162.0A patent/EP3800269B1/en active Active
- 2015-02-23 CN CN201580008133.6A patent/CN106687598B/zh active Active
- 2015-02-23 HK HK17105017.9A patent/HK1231512A1/zh unknown
- 2015-02-23 US US15/116,185 patent/US10246737B2/en active Active
- 2015-02-23 WO PCT/EP2015/053759 patent/WO2015124779A1/en not_active Ceased
- 2015-02-23 EP EP15707584.7A patent/EP3108008B1/en active Active
- 2015-02-23 EP EP24164295.8A patent/EP4400600A3/en active Pending
- 2015-02-23 CA CA2937832A patent/CA2937832C/en active Active
- 2015-02-23 JP JP2016570186A patent/JP6727137B2/ja active Active
-
2019
- 2019-04-01 US US16/371,268 patent/US10787700B2/en active Active
-
2020
- 2020-03-17 JP JP2020046081A patent/JP2020114225A/ja active Pending
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