CN106687598B - 检测样品中多种核苷酸的方法 - Google Patents

检测样品中多种核苷酸的方法 Download PDF

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Publication number
CN106687598B
CN106687598B CN201580008133.6A CN201580008133A CN106687598B CN 106687598 B CN106687598 B CN 106687598B CN 201580008133 A CN201580008133 A CN 201580008133A CN 106687598 B CN106687598 B CN 106687598B
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reporter
compound
nucleic acid
nucleotide
amount
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Chinese (zh)
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CN106687598A (zh
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乌利奇·托马斯
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Abbott Rapid Diagnostics Jena GmbH
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Alere Technologies GmbH
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0819—Microarrays; Biochips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0481—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure squeezing of channels or chambers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Dispersion Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201580008133.6A 2014-02-21 2015-02-23 检测样品中多种核苷酸的方法 Active CN106687598B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB1403076.1 2014-02-21
GBGB1403076.1A GB201403076D0 (en) 2014-02-21 2014-02-21 Methods for detecting multiple nucleic acids in a sample
PCT/EP2015/053759 WO2015124779A1 (en) 2014-02-21 2015-02-23 Methods for detecting multiple nucleic acids in a sample

Publications (2)

Publication Number Publication Date
CN106687598A CN106687598A (zh) 2017-05-17
CN106687598B true CN106687598B (zh) 2022-10-11

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CN201580008133.6A Active CN106687598B (zh) 2014-02-21 2015-02-23 检测样品中多种核苷酸的方法

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US (2) US10246737B2 (enExample)
EP (3) EP3108008B1 (enExample)
JP (2) JP6727137B2 (enExample)
CN (1) CN106687598B (enExample)
CA (1) CA2937832C (enExample)
GB (1) GB201403076D0 (enExample)
HK (1) HK1231512A1 (enExample)
WO (1) WO2015124779A1 (enExample)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201403076D0 (en) 2014-02-21 2014-04-09 ALERE TECHNOLOGIES GmbH Methods for detecting multiple nucleic acids in a sample
GB201713251D0 (en) * 2017-08-18 2017-10-04 Lgc Genomics Ltd Methods and kits for detection of nucleic acid molecules
CN110672569A (zh) * 2019-09-30 2020-01-10 中国科学院宁波工业技术研究院慈溪生物医学工程研究所 一种dna或rna检测体系、检测方法和它们的应用
CN112553376B (zh) * 2020-12-23 2023-06-02 四川大学 引物组、试剂盒、区分不同ibv疫苗毒株的多重pcr检测方法及其应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1352091A4 (en) * 2000-12-22 2004-07-21 Cepheid COMPOSITIONS AND METHODS FOR TOTAL INTERNAL MONITORING OF THE AMPLIFICATION REACTION
CN101553306A (zh) * 2006-11-06 2009-10-07 科隆迪亚戈有限公司 使用结合元件用于分析的装置和方法

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5538848A (en) * 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
ES2219374T3 (es) 1999-07-02 2004-12-01 Clondiag Chip Technologies Gmbh Dispositivo de matriz de microchip para la multiplicacion y la caracterizacion de acidos nucleicos.
US20020187488A1 (en) * 2001-01-30 2002-12-12 Chao Lin Quantitative assay for nucleic acids
JP2005516610A (ja) * 2001-07-15 2005-06-09 ケック グラデュエイト インスティテュート ニック形成剤を用いる遺伝子発現分析
US7226732B2 (en) * 2001-07-16 2007-06-05 Cepheid Methods, apparatus, and computer programs for verifying the integrity of a probe
EP1493822A1 (de) * 2003-07-02 2005-01-05 Labor Becker, Olgemoeller & Kollegen GbR Detektionsverfahren für Nucleinsäuren mit interner Amplifikationskontrolle
DE102004022263A1 (de) 2004-05-06 2005-12-15 Clondiag Chip Technologies Gmbh Vorrichtung und Verfahren zum Nachweis von molekularen Wechselwirkungen
KR101433208B1 (ko) * 2006-12-13 2014-08-22 루미넥스 코포레이션 실시간으로 ｐｃｒ의 다중 분석을 위한 시스템과 방법
ES2404067T3 (es) 2007-05-03 2013-05-23 Clondiag Gmbh Análisis
GB201403076D0 (en) 2014-02-21 2014-04-09 ALERE TECHNOLOGIES GmbH Methods for detecting multiple nucleic acids in a sample

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1352091A4 (en) * 2000-12-22 2004-07-21 Cepheid COMPOSITIONS AND METHODS FOR TOTAL INTERNAL MONITORING OF THE AMPLIFICATION REACTION
CN101553306A (zh) * 2006-11-06 2009-10-07 科隆迪亚戈有限公司 使用结合元件用于分析的装置和方法

Also Published As

Publication number Publication date
US10246737B2 (en) 2019-04-02
EP3108008A1 (en) 2016-12-28
HK1231512A1 (zh) 2017-12-22
CA2937832C (en) 2023-10-03
US10787700B2 (en) 2020-09-29
JP2017506914A (ja) 2017-03-16
US20190376125A1 (en) 2019-12-12
EP4400600A3 (en) 2024-10-23
EP3800269A1 (en) 2021-04-07
EP4400600A2 (en) 2024-07-17
GB201403076D0 (en) 2014-04-09
EP3108008B1 (en) 2020-10-28
CN106687598A (zh) 2017-05-17
WO2015124779A1 (en) 2015-08-27
JP6727137B2 (ja) 2020-07-22
JP2020114225A (ja) 2020-07-30
US20170081710A1 (en) 2017-03-23
CA2937832A1 (en) 2015-08-27
EP3800269B1 (en) 2024-03-20

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