JP6727137B2 - サンプル中の複数の核酸を検出するための方法 - Google Patents

サンプル中の複数の核酸を検出するための方法 Download PDF

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JP6727137B2
JP6727137B2 JP2016570186A JP2016570186A JP6727137B2 JP 6727137 B2 JP6727137 B2 JP 6727137B2 JP 2016570186 A JP2016570186 A JP 2016570186A JP 2016570186 A JP2016570186 A JP 2016570186A JP 6727137 B2 JP6727137 B2 JP 6727137B2
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nucleic acid
compound
reporter
amount
complex
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JP2017506914A (ja
JP2017506914A5 (enExample
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ウルリヒ，トーマス
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アボット・ラピッド・ダイアグノスティクス・イェーナ・ゲー・エム・ベー・ハー
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0819—Microarrays; Biochips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0481—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure squeezing of channels or chambers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Dispersion Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2016570186A 2014-02-21 2015-02-23 サンプル中の複数の核酸を検出するための方法 Active JP6727137B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB1403076.1 2014-02-21
GBGB1403076.1A GB201403076D0 (en) 2014-02-21 2014-02-21 Methods for detecting multiple nucleic acids in a sample
PCT/EP2015/053759 WO2015124779A1 (en) 2014-02-21 2015-02-23 Methods for detecting multiple nucleic acids in a sample

Related Child Applications (1)

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JP2020046081A Division JP2020114225A (ja) 2014-02-21 2020-03-17 サンプル中の複数の核酸を検出するための方法

Publications (3)

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JP2017506914A JP2017506914A (ja) 2017-03-16
JP2017506914A5 JP2017506914A5 (enExample) 2018-04-05
JP6727137B2 true JP6727137B2 (ja) 2020-07-22

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JP2016570186A Active JP6727137B2 (ja) 2014-02-21 2015-02-23 サンプル中の複数の核酸を検出するための方法
JP2020046081A Pending JP2020114225A (ja) 2014-02-21 2020-03-17 サンプル中の複数の核酸を検出するための方法

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US (2) US10246737B2 (enExample)
EP (3) EP3800269B1 (enExample)
JP (2) JP6727137B2 (enExample)
CN (1) CN106687598B (enExample)
CA (1) CA2937832C (enExample)
GB (1) GB201403076D0 (enExample)
HK (1) HK1231512A1 (enExample)
WO (1) WO2015124779A1 (enExample)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201403076D0 (en) 2014-02-21 2014-04-09 ALERE TECHNOLOGIES GmbH Methods for detecting multiple nucleic acids in a sample
GB201713251D0 (en) * 2017-08-18 2017-10-04 Lgc Genomics Ltd Methods and kits for detection of nucleic acid molecules
CN110672569A (zh) * 2019-09-30 2020-01-10 中国科学院宁波工业技术研究院慈溪生物医学工程研究所 一种dna或rna检测体系、检测方法和它们的应用
CN112553376B (zh) * 2020-12-23 2023-06-02 四川大学 引物组、试剂盒、区分不同ibv疫苗毒株的多重pcr检测方法及其应用

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5538848A (en) * 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
DE50006164D1 (de) 1999-07-02 2004-05-27 Clondiag Chip Tech Gmbh Microchip-matrix-vorrichtung zur vervielfältigung und charakterisierung von nukleinsäuren
US6312929B1 (en) * 2000-12-22 2001-11-06 Cepheid Compositions and methods enabling a totally internally controlled amplification reaction
US20020187488A1 (en) * 2001-01-30 2002-12-12 Chao Lin Quantitative assay for nucleic acids
EP1470250A2 (en) * 2001-07-15 2004-10-27 Keck Graduate Institute Gene expression analysis using nicking agents
US7226732B2 (en) * 2001-07-16 2007-06-05 Cepheid Methods, apparatus, and computer programs for verifying the integrity of a probe
EP1493822A1 (de) * 2003-07-02 2005-01-05 Labor Becker, Olgemoeller & Kollegen GbR Detektionsverfahren für Nucleinsäuren mit interner Amplifikationskontrolle
DE102004022263A1 (de) 2004-05-06 2005-12-15 Clondiag Chip Technologies Gmbh Vorrichtung und Verfahren zum Nachweis von molekularen Wechselwirkungen
WO2008055915A2 (en) 2006-11-06 2008-05-15 Clondiag Gmbh Device and process for assays using binding members
EP2099930B1 (en) * 2006-12-13 2015-02-18 Luminex Corporation Systems and methods for multiplex analysis of pcr in real time
CA2946024C (en) 2007-05-03 2020-06-02 Clondiag Gmbh Devices and methods for detecting analytes
GB201403076D0 (en) 2014-02-21 2014-04-09 ALERE TECHNOLOGIES GmbH Methods for detecting multiple nucleic acids in a sample

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Publication number Publication date
EP4400600A3 (en) 2024-10-23
JP2020114225A (ja) 2020-07-30
CA2937832A1 (en) 2015-08-27
EP3108008A1 (en) 2016-12-28
WO2015124779A1 (en) 2015-08-27
US10787700B2 (en) 2020-09-29
CA2937832C (en) 2023-10-03
EP3800269B1 (en) 2024-03-20
US20170081710A1 (en) 2017-03-23
US10246737B2 (en) 2019-04-02
JP2017506914A (ja) 2017-03-16
US20190376125A1 (en) 2019-12-12
EP4400600A2 (en) 2024-07-17
GB201403076D0 (en) 2014-04-09
EP3800269A1 (en) 2021-04-07
CN106687598A (zh) 2017-05-17
CN106687598B (zh) 2022-10-11
EP3108008B1 (en) 2020-10-28
HK1231512A1 (zh) 2017-12-22

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