JP2010131021A - 約15kDaおよび約45kDaの殺虫性タンパク質をコードする、植物において最も効果的に発現するポリヌクレオチド - Google Patents
約15kDaおよび約45kDaの殺虫性タンパク質をコードする、植物において最も効果的に発現するポリヌクレオチド Download PDFInfo
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Abstract
【解決手段】トウモロコシ等の植物に害虫抵抗性を付与するために植物を形質転換することができる、Bacillus thuringiensis由来の約15 kDaおよび約45 kDaの殺虫性タンパク質(δ−エンドトキシン)をコードする、特定の塩基配列を有するポリヌクレオチド、及び該殺虫性タンパク質。
【選択図】なし
Description
昆虫およびその他の有害生物のために、農家は年間10億ドルもの農作物の損失およびこれらの有害生物を制御するための出費を要する。農業生産環境において害虫によって引き起こされた損失には、農作物の収穫高の減少、農作物の品質の低下、および収穫にかかる費用の増加が含まれる。
本発明は、有害生物、特に植物有害生物の制御に有用な材料および方法に関する。より詳しく述べると、本発明は、殺虫性蛋白質をコードする新規の植物最適化ポリヌクレオチド配列を提供する。本発明のポリヌクレオチド配列は野生型配列と比較して特定の改変を有し、そのためにそれらは植物における最適化された発現に特に適している。当業者に公知の技術を用いて、本明細書に記述のポリヌクレオチド配列を、植物に害虫抵抗性を付与することを目的として植物を形質転換するために用いることができる。一つの好ましい態様において、本発明は約15kDaおよび約45kDaの殺虫性蛋白質をコードする植物最適化ポリヌクレオチド配列を提供する。
配列番号:1は、80JJ1-15-PO5として示される遺伝子のポリヌクレオチド配列であり、トウモロコシにおいて最も効果的に発現する。この遺伝子は、約15 kDaのタンパク質をコードする。この遺伝子とタンパク質は、国際公開公報第97/40162号に開示された。
本発明は、有害生物、特に植物有害生物の制御に有用な材料および方法に関する。より詳しく述べると、本発明は、殺虫性蛋白質をコードする新規の植物最適化ポリヌクレオチド配列を提供する。本発明のポリヌクレオチド配列は野生型配列と比較して特定の改変を有し、そのためにそれらは植物における最適化された発現に特に適している。本明細書に記載されているポリヌクレチド配列を用いて、当業者に公知の技術により、植物に害虫抵抗性を付与するために植物を形質転換することができる。好ましい態様において、本発明は、約15 kDaおよび約45 kDaの殺虫性タンパク質をコードする、植物において最も効果的に発現するポリヌクレオチド配列を提供する。
本発明の1つの局面は、殺虫性毒素をコードする本ポリヌクレオチド配列によって植物を形質転換することである。形質転換された植物は標的有害生物による攻撃に対して抵抗性である。本発明の遺伝子は植物において用いられるように最適化される。
Claims (25)
- 配列番号:2、配列番号:5、および配列番号:6からなる群より選択されるヌクレオチド配列を有するポリヌクレオチド。
- ヌクレオチド配列が配列番号:2である、請求項1記載のポリヌクレオチド。
- ヌクレオチド配列が配列番号:5である、請求項1記載のポリヌクレオチド。
- ヌクレオチド配列が配列番号:6である、請求項1記載のポリヌクレオチド。
- 請求項1記載のポリヌクレオチドを発現させる組換え宿主。
- ヌクレオチド配列が配列番号:2である、請求項5記載の宿主。
- ヌクレオチド配列が配列番号:5である、請求項5記載の宿主。
- ヌクレオチド配列が配列番号:6である、請求項5記載の宿主。
- 宿主が植物細胞である、請求項5記載の宿主。
- 宿主が植物である、請求項5記載の宿主。
- 宿主がトウモロコシである、請求項5記載の宿主。
- 請求項5記載の組換え宿主を生産する方法。
- 宿主が植物細胞である、請求項12記載の方法。
- 宿主が植物である、請求項12記載の方法。
- 宿主がトウモロコシである、請求項12記載の方法。
- 請求項1記載のポリヌクレオチドによりコードされ、該ポリヌクレオチドを発現させる組換え宿主により生産される第1のタンパク質に、害虫を接触させることを含む、植物の害虫を制御するための方法。
- 宿主が植物である、請求項16記載の方法。
- 宿主がトウモロコシ(Zea mays)植物である、請求項16記載の方法。
- ヌクレオチド配列が配列番号:2である、請求項18記載の方法。
- ヌクレオチド配列が配列番号:5である、請求項18記載の方法。
- ヌクレオチド配列が配列番号:6である、請求項18記載の方法。
- 約45 kDaの第2のタンパク質に害虫を接触させることをさらに含む、請求項19記載の方法。
- 約45 kDaの第2のタンパク質に害虫を接触させることをさらに含む、請求項20記載の方法。
- 約15 kDaの第2のタンパク質に該害虫を接触させることをさらに含む、請求項21記載の方法。
- 配列番号:3のアミノ酸配列を有する殺虫性タンパク質。
Applications Claiming Priority (4)
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US10540898P | 1998-10-23 | 1998-10-23 | |
US10535998P | 1998-10-23 | 1998-10-23 | |
US60/105,408 | 1998-10-23 | ||
US60/105,359 | 1998-10-23 |
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JP2000578456A Division JP4647099B2 (ja) | 1998-10-23 | 1999-10-21 | 約15kDaおよび約45kDaの殺虫性タンパク質をコードする、植物において最も効果的に発現するポリヌクレオチド |
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JP2010131021A true JP2010131021A (ja) | 2010-06-17 |
JP4912473B2 JP4912473B2 (ja) | 2012-04-11 |
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JP2000578456A Expired - Lifetime JP4647099B2 (ja) | 1998-10-23 | 1999-10-21 | 約15kDaおよび約45kDaの殺虫性タンパク質をコードする、植物において最も効果的に発現するポリヌクレオチド |
JP2010001776A Expired - Lifetime JP4912473B2 (ja) | 1998-10-23 | 2010-01-07 | 約15kDaおよび約45kDaの殺虫性タンパク質をコードする、植物において最も効果的に発現するポリヌクレオチド |
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JP2000578456A Expired - Lifetime JP4647099B2 (ja) | 1998-10-23 | 1999-10-21 | 約15kDaおよび約45kDaの殺虫性タンパク質をコードする、植物において最も効果的に発現するポリヌクレオチド |
Country Status (13)
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US (1) | US6340593B1 (ja) |
EP (1) | EP1124967B1 (ja) |
JP (2) | JP4647099B2 (ja) |
KR (1) | KR20010073216A (ja) |
CN (1) | CN1324407A (ja) |
AR (2) | AR020944A1 (ja) |
AT (1) | ATE327333T1 (ja) |
AU (1) | AU768246B2 (ja) |
BR (1) | BR9914746A (ja) |
CA (2) | CA2703161C (ja) |
DE (2) | DE69940962D1 (ja) |
ES (2) | ES2327948T3 (ja) |
WO (1) | WO2000024904A1 (ja) |
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Also Published As
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JP4647099B2 (ja) | 2011-03-09 |
EP1124967A1 (en) | 2001-08-22 |
JP4912473B2 (ja) | 2012-04-11 |
WO2000024904A1 (en) | 2000-05-04 |
BR9914746A (pt) | 2001-10-30 |
AU768246B2 (en) | 2003-12-04 |
ATE327333T1 (de) | 2006-06-15 |
ES2264283T3 (es) | 2006-12-16 |
CA2703161C (en) | 2011-12-20 |
AR020944A1 (es) | 2002-06-05 |
KR20010073216A (ko) | 2001-07-31 |
EP1124967B1 (en) | 2006-05-24 |
DE69940962D1 (de) | 2009-07-16 |
ES2327948T3 (es) | 2009-11-05 |
CN1324407A (zh) | 2001-11-28 |
CA2345905A1 (en) | 2000-05-04 |
DE69931511D1 (de) | 2006-06-29 |
DE69931511T2 (de) | 2006-09-28 |
CA2345905C (en) | 2010-08-03 |
JP2002528083A (ja) | 2002-09-03 |
AU1218100A (en) | 2000-05-15 |
CA2703161A1 (en) | 2000-05-04 |
AR066201A2 (es) | 2009-08-05 |
US6340593B1 (en) | 2002-01-22 |
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