JP2009535064A5 - - Google Patents

Info

Publication number

JP2009535064A5

JP2009535064A5 JP2009509717A JP2009509717A JP2009535064A5 JP 2009535064 A5 JP2009535064 A5 JP 2009535064A5 JP 2009509717 A JP2009509717 A JP 2009509717A JP 2009509717 A JP2009509717 A JP 2009509717A JP 2009535064 A5 JP2009535064 A5 JP 2009535064A5

Authority

JP

Japan

Prior art keywords

seq

host cell

butanone

butanediol

acetoin

Prior art date

2007-05-02

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Granted

Links

• Espacenet
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• 238000000034 method Methods 0.000 claims description 47
• BTANRVKWQNVYAZ-UHFFFAOYSA-N butan-2-ol Chemical compound CCC(C)O BTANRVKWQNVYAZ-UHFFFAOYSA-N 0.000 claims description 42
• ROWKJAVDOGWPAT-UHFFFAOYSA-N Acetoin Chemical compound CC(O)C(C)=O ROWKJAVDOGWPAT-UHFFFAOYSA-N 0.000 claims description 40
• ZWEHNKRNPOVVGH-UHFFFAOYSA-N 2-Butanone Chemical compound CCC(C)=O ZWEHNKRNPOVVGH-UHFFFAOYSA-N 0.000 claims description 25
• 239000000758 substrate Substances 0.000 claims description 23
• OWBTYPJTUOEWEK-UHFFFAOYSA-N butane-2,3-diol Chemical compound CC(O)C(C)O OWBTYPJTUOEWEK-UHFFFAOYSA-N 0.000 claims description 20
• GFAZHVHNLUBROE-UHFFFAOYSA-N hydroxymethyl propionaldehyde Natural products CCC(=O)CO GFAZHVHNLUBROE-UHFFFAOYSA-N 0.000 claims description 20
• 238000006243 chemical reaction Methods 0.000 claims description 18
• 229920001184 polypeptide Polymers 0.000 claims description 18
• 102000004196 processed proteins & peptides Human genes 0.000 claims description 18
• 108090000765 processed proteins & peptides Proteins 0.000 claims description 18
• 108020004414 DNA Proteins 0.000 claims description 16
• 102000053602 DNA Human genes 0.000 claims description 12
• LCTONWCANYUPML-UHFFFAOYSA-N Pyruvic acid Chemical compound CC(=O)C(O)=O LCTONWCANYUPML-UHFFFAOYSA-N 0.000 claims description 12
• 230000000813 microbial effect Effects 0.000 claims description 10
• 238000000855 fermentation Methods 0.000 claims description 8
• 230000004151 fermentation Effects 0.000 claims description 8
• 244000005700 microbiome Species 0.000 claims description 8
• 229940107700 pyruvic acid Drugs 0.000 claims description 6
• OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 5
• 229910052799 carbon Inorganic materials 0.000 claims description 5
• LCTONWCANYUPML-UHFFFAOYSA-M Pyruvate Chemical compound CC(=O)C([O-])=O LCTONWCANYUPML-UHFFFAOYSA-M 0.000 claims description 4
• 238000004519 manufacturing process Methods 0.000 claims description 4
• 229940076788 pyruvate Drugs 0.000 claims description 4
• 125000003275 alpha amino acid group Chemical group 0.000 description 45
• 108010025885 Glycerol dehydratase Proteins 0.000 description 26
• 108010065027 Propanediol Dehydratase Proteins 0.000 description 26
• 108090000623 proteins and genes Proteins 0.000 description 20
• 102000004169 proteins and genes Human genes 0.000 description 20
• 239000011159 matrix material Substances 0.000 description 18
• 239000002609 medium Substances 0.000 description 16
• 150000002009 diols Chemical class 0.000 description 8
• 108010000700 Acetolactate synthase Proteins 0.000 description 7
• 241000235648 Pichia Species 0.000 description 4
• 108010084631 acetolactate decarboxylase Proteins 0.000 description 4
• 108010036467 butanediol dehydrogenase Proteins 0.000 description 4
• 108010057307 butanol dehydrogenase Proteins 0.000 description 4
• 241000588747 Klebsiella pneumoniae Species 0.000 description 3
• 101150077640 budB gene Proteins 0.000 description 3
• FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 2
• 241000588986 Alcaligenes Species 0.000 description 2
• 241000186063 Arthrobacter Species 0.000 description 2
• 241000193830 Bacillus <bacterium> Species 0.000 description 2
• 241000894006 Bacteria Species 0.000 description 2
• 241000186146 Brevibacterium Species 0.000 description 2
• 241000222120 Candida <Saccharomycetales> Species 0.000 description 2
• 241000193403 Clostridium Species 0.000 description 2
• 241000186216 Corynebacterium Species 0.000 description 2
• 241000192700 Cyanobacteria Species 0.000 description 2
• 241000194033 Enterococcus Species 0.000 description 2
• 241000588722 Escherichia Species 0.000 description 2
• 241000233866 Fungi Species 0.000 description 2
• 241000588748 Klebsiella Species 0.000 description 2
• 241000186660 Lactobacillus Species 0.000 description 2
• 108700026244 Open Reading Frames Proteins 0.000 description 2
• 241000179039 Paenibacillus Species 0.000 description 2
• 241000192001 Pediococcus Species 0.000 description 2
• 241000589516 Pseudomonas Species 0.000 description 2
• 241000316848 Rhodococcus <scale insect> Species 0.000 description 2
• 241000235070 Saccharomyces Species 0.000 description 2
• 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
• 241000607142 Salmonella Species 0.000 description 2
• 241000588901 Zymomonas Species 0.000 description 2
• 239000002253 acid Substances 0.000 description 2
• 150000001413 amino acids Chemical class 0.000 description 2
• 229940039696 lactobacillus Drugs 0.000 description 2
• 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
• 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
• 102000004190 Enzymes Human genes 0.000 description 1
• 108090000790 Enzymes Proteins 0.000 description 1
• 241000588724 Escherichia coli Species 0.000 description 1
• 238000012408 PCR amplification Methods 0.000 description 1
• 230000003321 amplification Effects 0.000 description 1
• 239000003153 chemical reaction reagent Substances 0.000 description 1
• 238000010367 cloning Methods 0.000 description 1
• 150000004676 glycans Chemical class 0.000 description 1
• 239000001963 growth medium Substances 0.000 description 1
• 150000002772 monosaccharides Chemical class 0.000 description 1
• 238000003199 nucleic acid amplification method Methods 0.000 description 1
• 239000002773 nucleotide Substances 0.000 description 1
• 125000003729 nucleotide group Chemical group 0.000 description 1
• 229920001542 oligosaccharide Polymers 0.000 description 1
• 150000002482 oligosaccharides Chemical class 0.000 description 1
• 229920001282 polysaccharide Polymers 0.000 description 1
• 239000005017 polysaccharide Substances 0.000 description 1