JP2009507478A5 - - Google Patents

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JP2009507478A5
JP2009507478A5 JP2008529643A JP2008529643A JP2009507478A5 JP 2009507478 A5 JP2009507478 A5 JP 2009507478A5 JP 2008529643 A JP2008529643 A JP 2008529643A JP 2008529643 A JP2008529643 A JP 2008529643A JP 2009507478 A5 JP2009507478 A5 JP 2009507478A5
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Japan
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nucleic acid
polymerase
temperature
reaction
cooling
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JP2008529643A
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JP5330830B2 (ja
JP2009507478A (ja
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Priority claimed from EP05019665A external-priority patent/EP1762627A1/de
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Claims (11)

  1. （ａ）核酸を温度５５℃から８０℃へ加熱、
    （ｂ）温度４から４５℃へ冷却、および
    （ｃ）ポリメラーゼの核酸への添加によってポリメラーゼ反応を開始
    ：の段階を含むポリメラーゼ反応のために核酸を活性化する方法。
  2. 段階（ｃ）でのポリメラーゼが熱不安定性ポリメラーゼである点で特徴づけられる、請求項１に記載の方法。
  3. ポリメラーゼ反応が鎖置換反応である点で特徴づけられる、請求項１または２に記載の方法。
  4. 鎖置換反応が多置換反応である点で特徴づけられる、請求項３に記載の方法。
  5. 核酸がＤＮＡである点で特徴づけられる、請求項１〜４のいずれか一つに記載の方法。
  6. 段階（ａ）での核酸が温度６０℃から７０℃へ加熱される点で特徴づけられる、請求項１〜５のいずれか一つに記載の方法。
  7. 核酸が水溶液中に精製された形で存在する点で特徴づけられる、請求項１〜５のいずれか一つに記載の方法。
  8. 核酸が細胞内に存在する点で特徴づけられる、請求項１から６のいずれか一つに記載の方法。
  9. 段階（ｂ）での核酸の冷却を１５℃から４２℃の温度で行う点で特徴づけられる、請求項１〜８のいずれか一つに記載の方法。
  10. 段階（ｂ）での核酸の冷却を２５から３７℃の温度で行う点で特徴づけられる、請求項１〜８のいずれか一つに記載の方法。
  11. 段階（ａ）での核酸が温度６５℃へ加熱される点で特徴づけられる、請求項１〜１０のいずれか一つに記載の方法。
JP2008529643A 2005-09-09 2006-09-11 ポリメラーゼ反応のために核酸を活性化する方法 Active JP5330830B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP05019665.8 2005-09-09
EP05019665A EP1762627A1 (de) 2005-09-09 2005-09-09 Verfahren zur Aktivierung einer Nukleinsäure für eine Polymerase-Reaktion
PCT/EP2006/066223 WO2007028833A2 (de) 2005-09-09 2006-09-11 Verfahren zur aktivierung einer nukleinsäure für eine polymerase-reaktion

Publications (3)

Publication Number Publication Date
JP2009507478A JP2009507478A (ja) 2009-02-26
JP2009507478A5 true JP2009507478A5 (ja) 2009-09-10
JP5330830B2 JP5330830B2 (ja) 2013-10-30

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Family Applications (1)

Application Number Title Priority Date Filing Date
JP2008529643A Active JP5330830B2 (ja) 2005-09-09 2006-09-11 ポリメラーゼ反応のために核酸を活性化する方法

Country Status (5)

Country Link
US (1) US9683255B2 (ja)
EP (2) EP1762627A1 (ja)
JP (1) JP5330830B2 (ja)
AT (1) ATE513928T1 (ja)
WO (1) WO2007028833A2 (ja)

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GB201611469D0 (en) 2016-06-30 2016-08-17 Lumiradx Tech Ltd Improvements in or relating to nucleic acid amplification processes
GB2569965A (en) 2018-01-04 2019-07-10 Lumiradx Uk Ltd Improvements in or relating to amplification of nucleic acids
CA3226451A1 (en) 2021-07-21 2023-01-26 Sam Reed Method and system comprising a cartridge for sequencing target polynucleotides
GB202110479D0 (en) 2021-07-21 2021-09-01 Dnae Diagnostics Ltd Compositions, kits and methods for sequencing target polynucleotides
GB202110485D0 (en) 2021-07-21 2021-09-01 Dnae Diagnostics Ltd Compositions, kits and methods for sequencing target polynucleotides

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