JP2009195255A5 - - Google Patents
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- Publication number
- JP2009195255A5 JP2009195255A5 JP2009139486A JP2009139486A JP2009195255A5 JP 2009195255 A5 JP2009195255 A5 JP 2009195255A5 JP 2009139486 A JP2009139486 A JP 2009139486A JP 2009139486 A JP2009139486 A JP 2009139486A JP 2009195255 A5 JP2009195255 A5 JP 2009195255A5
- Authority
- JP
- Japan
- Prior art keywords
- sucrose phosphorylase
- amino acid
- seq
- position corresponding
- acid residue
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 108020000005 Sucrose phosphorylase Proteins 0.000 claims 129
- 125000000539 amino acid group Chemical group 0.000 claims 69
- 238000000034 method Methods 0.000 claims 29
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 claims 26
- 235000004400 serine Nutrition 0.000 claims 26
- AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 claims 24
- 150000001413 amino acids Chemical group 0.000 claims 24
- AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 claims 24
- 229960000310 isoleucine Drugs 0.000 claims 24
- 235000014705 isoleucine Nutrition 0.000 claims 24
- 102000004190 Enzymes Human genes 0.000 claims 20
- 108090000790 Enzymes Proteins 0.000 claims 20
- 230000000694 effects Effects 0.000 claims 20
- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 claims 19
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 claims 19
- 239000004472 Lysine Substances 0.000 claims 19
- KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 claims 19
- 235000018977 lysine Nutrition 0.000 claims 19
- 239000004474 valine Substances 0.000 claims 19
- 235000014393 valine Nutrition 0.000 claims 19
- 238000010438 heat treatment Methods 0.000 claims 18
- 239000004475 Arginine Substances 0.000 claims 16
- 235000009697 arginine Nutrition 0.000 claims 16
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 claims 16
- 235000014304 histidine Nutrition 0.000 claims 16
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 claims 16
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 claims 15
- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 claims 15
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 claims 15
- MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 claims 14
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 14
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 claims 14
- 235000013930 proline Nutrition 0.000 claims 14
- DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 claims 13
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 claims 13
- 235000009582 asparagine Nutrition 0.000 claims 13
- 229960001230 asparagine Drugs 0.000 claims 13
- 150000007523 nucleic acids Chemical class 0.000 claims 13
- 102000039446 nucleic acids Human genes 0.000 claims 13
- 108020004707 nucleic acids Proteins 0.000 claims 13
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 claims 12
- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 claims 10
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 claims 10
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 claims 10
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 claims 10
- 241000194017 Streptococcus Species 0.000 claims 10
- 239000007983 Tris buffer Substances 0.000 claims 10
- 235000018417 cysteine Nutrition 0.000 claims 10
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 claims 10
- 235000005772 leucine Nutrition 0.000 claims 10
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims 10
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 claims 10
- 235000004554 glutamine Nutrition 0.000 claims 9
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 claims 9
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims 8
- 229930006000 Sucrose Natural products 0.000 claims 8
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims 8
- HXXFSFRBOHSIMQ-VFUOTHLCSA-N alpha-D-glucose 1-phosphate Chemical compound OC[C@H]1O[C@H](OP(O)(O)=O)[C@H](O)[C@@H](O)[C@@H]1O HXXFSFRBOHSIMQ-VFUOTHLCSA-N 0.000 claims 8
- 229950010772 glucose-1-phosphate Drugs 0.000 claims 8
- 239000005720 sucrose Substances 0.000 claims 8
- 108010073135 Phosphorylases Proteins 0.000 claims 7
- 102000009097 Phosphorylases Human genes 0.000 claims 7
- 241000194019 Streptococcus mutans Species 0.000 claims 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims 5
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 claims 5
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 claims 5
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 claims 5
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 claims 5
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 claims 5
- 239000004473 Threonine Substances 0.000 claims 5
- 235000004279 alanine Nutrition 0.000 claims 5
- 239000008103 glucose Substances 0.000 claims 5
- 229930182817 methionine Natural products 0.000 claims 5
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 claims 5
- 229920000642 polymer Polymers 0.000 claims 5
- 239000004471 Glycine Substances 0.000 claims 4
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 claims 4
- 241000192130 Leuconostoc mesenteroides Species 0.000 claims 4
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims 4
- 235000003704 aspartic acid Nutrition 0.000 claims 4
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 claims 4
- 239000013604 expression vector Substances 0.000 claims 4
- 239000000243 solution Substances 0.000 claims 4
- 230000002194 synthesizing effect Effects 0.000 claims 4
- 241000202223 Oenococcus Species 0.000 claims 2
- 241001134658 Streptococcus mitis Species 0.000 claims 2
- 241000193998 Streptococcus pneumoniae Species 0.000 claims 2
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims 2
- 229910052816 inorganic phosphate Inorganic materials 0.000 claims 2
- 229940031000 streptococcus pneumoniae Drugs 0.000 claims 2
- 239000000758 substrate Substances 0.000 claims 2
- 229920000310 Alpha glucan Polymers 0.000 claims 1
- 108010074725 Alpha,alpha-trehalose phosphorylase Proteins 0.000 claims 1
- 108050004944 Alpha-glucan phosphorylases Proteins 0.000 claims 1
- 229920002498 Beta-glucan Polymers 0.000 claims 1
- 108010077004 Cellodextrin phosphorylase Proteins 0.000 claims 1
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 claims 1
- 108010037131 Laminaribiose phosphorylase Proteins 0.000 claims 1
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 claims 1
- 108010048610 cellobiose phosphorylase Proteins 0.000 claims 1
- 239000013598 vector Substances 0.000 claims 1
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2009139486A JP2009195255A (ja) | 2003-09-04 | 2009-06-10 | スクロースホスホリラーゼ（ｓｐ）の耐熱化方法 |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2003313305 | 2003-09-04 | ||
| JP2009139486A JP2009195255A (ja) | 2003-09-04 | 2009-06-10 | スクロースホスホリラーゼ（ｓｐ）の耐熱化方法 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2005513633A Division JP4450392B2 (ja) | 2003-09-04 | 2004-08-31 | スクロースホスホリラーゼ（ｓｐ）の耐熱化方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2009195255A JP2009195255A (ja) | 2009-09-03 |
| JP2009195255A5 true JP2009195255A5 (https=) | 2010-06-17 |
Family
ID=34269765
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2005513633A Expired - Lifetime JP4450392B2 (ja) | 2003-09-04 | 2004-08-31 | スクロースホスホリラーゼ（ｓｐ）の耐熱化方法 |
| JP2009139486A Pending JP2009195255A (ja) | 2003-09-04 | 2009-06-10 | スクロースホスホリラーゼ（ｓｐ）の耐熱化方法 |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2005513633A Expired - Lifetime JP4450392B2 (ja) | 2003-09-04 | 2004-08-31 | スクロースホスホリラーゼ（ｓｐ）の耐熱化方法 |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US7968309B2 (https=) |
| EP (1) | EP1661985B1 (https=) |
| JP (2) | JP4450392B2 (https=) |
| CN (1) | CN1845990B (https=) |
| AU (1) | AU2004271018B2 (https=) |
| DK (1) | DK1661985T3 (https=) |
| WO (1) | WO2005024008A1 (https=) |
Families Citing this family (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010146875A1 (ja) | 2009-06-18 | 2010-12-23 | オーミケンシ株式会社 | ヨウ素およびアミロースを含有する繊維、その製造法およびその利用 |
| JP5955314B2 (ja) * | 2010-04-06 | 2016-07-20 | ウニベルズィタイト・ヘント | 熱安定性スクロースホスホリラーゼ |
| SG186836A1 (en) | 2010-07-12 | 2013-02-28 | Univ Gent | Metabolically engineered organisms for the production of added value bio-products |
| CN102021152B (zh) * | 2010-10-14 | 2012-01-11 | 广西大学 | 蔗糖磷酸化酶的突变体及其应用 |
| CN107109450A (zh) * | 2014-12-23 | 2017-08-29 | 纳幕尔杜邦公司 | 酶促产生的纤维素 |
| KR20170008367A (ko) * | 2015-07-13 | 2017-01-24 | 씨제이제일제당 (주) | 글루코스 전이 활성을 가지는 단백질 및 이의 용도 |
| CN105779404B (zh) * | 2016-05-11 | 2019-04-16 | 广西大学 | 一种蔗糖磷酸化酶的突变体t155s及其应用 |
| TWI871271B (zh) * | 2016-11-28 | 2025-02-01 | 德商Ｃ 樂克塔股份有限公司 | 海藻糖磷酸化酶 |
| CN107236696B (zh) * | 2017-07-31 | 2019-11-26 | 江南大学 | 一种表达L.mesenteroides来源的蔗糖磷酸化酶重组枯草芽孢杆菌 |
| CN112004939A (zh) * | 2018-04-23 | 2020-11-27 | 丹尼斯科美国公司 | 用磷酸化酶合成包含β-1,3糖苷键的葡聚糖 |
| EP3827090A1 (en) | 2018-07-23 | 2021-06-02 | Danisco US Inc. | Alpha-glucose-1 -phosphate synthesis from sucrose and glucan synthesis using glucan phosphorylases |
| CN109694892B (zh) * | 2018-11-27 | 2021-03-23 | 北京工商大学 | 制备红景天苷的方法和试剂盒 |
| CN109988778B (zh) * | 2019-05-14 | 2020-10-27 | 南京工业大学 | 一种蔗糖磷酸化酶基因及其应用 |
| WO2021003132A1 (en) * | 2019-07-02 | 2021-01-07 | Codexis, Inc. | Engineered sucrose phosphorylase variant enzymes |
| CN110734899B (zh) * | 2019-10-31 | 2021-06-18 | 江南大学 | 一种酶活提高的蔗糖磷酸化酶突变体及其构建方法和应用 |
| CN112300977B (zh) * | 2020-11-18 | 2024-01-12 | 江南大学 | 合成α-熊果苷的基因工程菌株及其构建方法与应用 |
| CN112375724B (zh) * | 2020-11-18 | 2022-12-16 | 江南大学 | 高效合成α-熊果苷的基因工程菌及其构建方法与应用 |
| JP7650668B2 (ja) * | 2021-01-12 | 2025-03-25 | 第一工業製薬株式会社 | セロオリゴ糖の製造方法 |
| WO2023236204A1 (en) * | 2022-06-10 | 2023-12-14 | Beren Therapeutics P.B.C. | Methods for producing beta-cyclodextrins |
| CN115044568B (zh) * | 2022-06-20 | 2024-01-23 | 华熙生物科技股份有限公司 | 高稳定型的蔗糖磷酸化酶突变体及其应用 |
| CN116144625A (zh) * | 2023-02-24 | 2023-05-23 | 杭州佳嘉乐生物技术有限公司 | 重组蔗糖磷酸化酶及其催化糖基化反应的方法 |
| CN116731997A (zh) * | 2023-06-19 | 2023-09-12 | 暨南大学 | 一种定点突变的蔗糖磷酸化酶及其应用 |
| CN116987684B (zh) * | 2023-08-08 | 2024-04-26 | 浙江赞源生物技术有限公司 | 蔗糖磷酸化酶突变体及其在制备甘油葡糖苷中的应用 |
| CN119842654B (zh) * | 2024-12-31 | 2025-08-05 | 南京诺云生物科技有限公司 | 一种耐热性增强的蔗糖磷酸化酶及其在高温催化制备α-熊果苷中的应用 |
| CN120230733B (zh) * | 2025-06-03 | 2025-07-29 | 山东三元生物科技股份有限公司 | 一种基于Spytag/SpyCatcher环化修饰的蔗糖磷酸化酶突变体及其应用 |
| CN120230734B (zh) * | 2025-06-03 | 2025-07-29 | 山东三元生物科技股份有限公司 | 蔗糖磷酸化酶突变体及其在合成2-O-α-D-吡喃葡萄糖基-L-抗坏血酸中的应用 |
| CN121320295B (zh) * | 2025-12-16 | 2026-03-24 | 山东三元生物科技股份有限公司 | 一种蔗糖磷酸化酶突变体及其在α-熊果苷合成中的应用 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1180380A (zh) * | 1995-02-10 | 1998-04-29 | 孟山都公司 | 植物中蔗糖磷酸化酶的表达 |
| GB0107658D0 (en) * | 2001-03-27 | 2001-05-16 | Chiron Spa | Streptococcus pneumoniae |
| KR100995248B1 (ko) * | 2001-05-28 | 2010-11-18 | 에자끼구리고가부시키가이샤 | 글루칸의 생성방법 및 제조방법 |
-
2004
- 2004-08-31 EP EP04772489.3A patent/EP1661985B1/en not_active Expired - Lifetime
- 2004-08-31 DK DK04772489.3T patent/DK1661985T3/en active
- 2004-08-31 WO PCT/JP2004/012533 patent/WO2005024008A1/ja not_active Ceased
- 2004-08-31 JP JP2005513633A patent/JP4450392B2/ja not_active Expired - Lifetime
- 2004-08-31 AU AU2004271018A patent/AU2004271018B2/en not_active Ceased
- 2004-08-31 US US10/566,224 patent/US7968309B2/en active Active
- 2004-08-31 CN CN2004800253207A patent/CN1845990B/zh not_active Expired - Fee Related
-
2009
- 2009-06-10 JP JP2009139486A patent/JP2009195255A/ja active Pending
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