SG10201504733XA - Metabolically engineered organisms for the production of added value bio-products - Google Patents
Metabolically engineered organisms for the production of added value bio-productsInfo
- Publication number
- SG10201504733XA SG10201504733XA SG10201504733XA SG10201504733XA SG10201504733XA SG 10201504733X A SG10201504733X A SG 10201504733XA SG 10201504733X A SG10201504733X A SG 10201504733XA SG 10201504733X A SG10201504733X A SG 10201504733XA SG 10201504733X A SG10201504733X A SG 10201504733XA
- Authority
- SG
- Singapore
- Prior art keywords
- products
- production
- added value
- metabolically engineered
- engineered organisms
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2431—Beta-fructofuranosidase (3.2.1.26), i.e. invertase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/90—Isomerases (5.)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/305—Pyrimidine nucleotides
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/01—Hexosyltransferases (2.4.1)
- C12Y204/01007—Sucrose phosphorylase (2.4.1.7)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/01—Hexosyltransferases (2.4.1)
- C12Y204/01008—Maltose phosphorylase (2.4.1.8)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/01—Hexosyltransferases (2.4.1)
- C12Y204/01022—Lactose synthase (2.4.1.22)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/01—Hexosyltransferases (2.4.1)
- C12Y204/01069—Galactoside 2-alpha-L-fucosyltransferase (2.4.1.69)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01026—Beta-fructofuranosidase (3.2.1.26), i.e. invertase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y504/00—Intramolecular transferases (5.4)
- C12Y504/02—Phosphotransferases (phosphomutases) (5.4.2)
- C12Y504/02002—Phosphoglucomutase (5.4.2.2)
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Mycology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP10169304 | 2010-07-12 |
Publications (1)
Publication Number | Publication Date |
---|---|
SG10201504733XA true SG10201504733XA (en) | 2015-07-30 |
Family
ID=44060925
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SG2012095741A SG186836A1 (en) | 2010-07-12 | 2011-07-12 | Metabolically engineered organisms for the production of added value bio-products |
SG10201504733XA SG10201504733XA (en) | 2010-07-12 | 2011-07-12 | Metabolically engineered organisms for the production of added value bio-products |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SG2012095741A SG186836A1 (en) | 2010-07-12 | 2011-07-12 | Metabolically engineered organisms for the production of added value bio-products |
Country Status (15)
Country | Link |
---|---|
US (4) | US9701992B2 (en) |
EP (3) | EP3235907B1 (en) |
JP (3) | JP5998130B2 (en) |
CN (3) | CN107603935A (en) |
AU (1) | AU2011278315B2 (en) |
CA (1) | CA2804713C (en) |
DE (1) | DE202011111144U1 (en) |
DK (1) | DK3235907T3 (en) |
ES (1) | ES2955617T3 (en) |
FI (1) | FI3235907T3 (en) |
MX (7) | MX353506B (en) |
MY (1) | MY185012A (en) |
NZ (1) | NZ605093A (en) |
SG (2) | SG186836A1 (en) |
WO (1) | WO2012007481A2 (en) |
Families Citing this family (70)
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US8647642B2 (en) | 2008-09-18 | 2014-02-11 | Aviex Technologies, Llc | Live bacterial vaccines resistant to carbon dioxide (CO2), acidic PH and/or osmolarity for viral infection prophylaxis or treatment |
WO2012112777A2 (en) * | 2011-02-16 | 2012-08-23 | Glycosyn LLC | Biosynthesis of human milk oligosaccharides in engineered bacteria |
KR101989637B1 (en) | 2011-07-12 | 2019-06-14 | 사이언티스트 오브 포춘 에스.에이. | Recombinant microorganism for the production of useful metabolites |
US20130203136A1 (en) | 2011-07-27 | 2013-08-08 | Alliance For Sustainable Energy, Llc | Biological production of organic compounds |
US9914947B2 (en) | 2011-07-27 | 2018-03-13 | Alliance For Sustainable Energy, Llc | Biological production of organic compounds |
JP6033632B2 (en) * | 2012-10-15 | 2016-11-30 | 国立大学法人 新潟大学 | Method for producing acidic β-glucosyl disaccharide using cellobionate phosphorylase |
US9944965B2 (en) * | 2012-12-20 | 2018-04-17 | The Board Of Trustees Of The University Of Illinois | Biosynthesis of oligosaccharides |
EP2927316B1 (en) * | 2014-03-31 | 2018-11-07 | Jennewein Biotechnologie GmbH | Total fermentation of oligosaccharides |
WO2015188834A1 (en) | 2014-06-11 | 2015-12-17 | Glycom A/S | Separation of 2'-o-fucosyllactose from fermentation broth |
WO2015197082A1 (en) | 2014-06-27 | 2015-12-30 | Glycom A/S | Oligosaccharide production |
US11926858B2 (en) | 2014-06-27 | 2024-03-12 | Glycom A/S | Oligosaccharide production |
CN104611249B (en) * | 2014-11-17 | 2019-06-11 | 中国科学院天津工业生物技术研究所 | A method of D-Psicose is synthesized using the full cell of aldolase |
DE202015009775U1 (en) | 2014-12-16 | 2020-02-05 | Glycom A/S | Separation of 2'-FL from a fermentation broth |
WO2016153300A1 (en) * | 2015-03-24 | 2016-09-29 | 서울대학교 산학협력단 | 2-fucosyllactose producing mutant microorganisms and method for producing 2-fucosyllactose using same |
EP3322803B1 (en) | 2015-10-02 | 2021-03-24 | Bonumose LLC | Enzymatic production of d-tagatose |
US10997364B2 (en) | 2015-11-02 | 2021-05-04 | Microsoft Technology Licensing, Llc | Operations on sound files associated with cells in spreadsheets |
US20190000116A1 (en) * | 2015-11-12 | 2019-01-03 | Petiva Private Limited | Process for Preparing Non-Cariogenic, Sustained Energy Release Juice |
ES2856749T3 (en) | 2016-10-29 | 2021-09-28 | Chr Hansen Hmo Gmbh | Process for the production of fucosylated oligosaccharides |
US11129906B1 (en) | 2016-12-07 | 2021-09-28 | David Gordon Bermudes | Chimeric protein toxins for expression by therapeutic bacteria |
US11180535B1 (en) | 2016-12-07 | 2021-11-23 | David Gordon Bermudes | Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria |
US11535878B2 (en) | 2016-12-27 | 2022-12-27 | Inbiose N.V. | In vivo synthesis of sialylated compounds |
JP6878607B2 (en) * | 2017-02-21 | 2021-05-26 | デューク ユニバーシティ | Compositions and Methods for Robust Dynamic Metabolic Control |
US10767205B2 (en) | 2017-03-07 | 2020-09-08 | DePuy Synthes Products, Inc. | Bacterium for the production of an exopolysaccharide comprising N-acetylglucosamine and D-glucose |
US10633682B2 (en) | 2017-03-07 | 2020-04-28 | DePuy Synthes Products, Inc. | Bioresorbable exopolysaccharides |
EP3596212A4 (en) * | 2017-03-13 | 2021-06-02 | Bonumose LLC | Enzymatic production of hexoses |
JP2021513862A (en) * | 2018-02-23 | 2021-06-03 | ダニスコ・ユーエス・インク | Synthesis of glucans containing alpha-1,3 glycosidic bonds by phosphorylase enzymes |
US20190323053A1 (en) * | 2018-04-23 | 2019-10-24 | Dupont Nutrition Biosciences Aps | Increasing activity of 2? fucosyllactose transporters endogenous to microbial cells |
US11541105B2 (en) | 2018-06-01 | 2023-01-03 | The Research Foundation For The State University Of New York | Compositions and methods for disrupting biofilm formation and maintenance |
ES2965229T3 (en) * | 2018-09-06 | 2024-04-11 | Chr Hansen Hmo Gmbh | Fermentative production of oligosaccharides by total fermentation using a mixed raw material |
WO2020058493A1 (en) | 2018-09-21 | 2020-03-26 | Universiteit Gent | Method for the production of amino sugar containing products |
KR20210080420A (en) | 2018-10-18 | 2021-06-30 | 바스프 에스이 | Method for obtaining crystalline 2'-fucosyllactose |
SG11202106291RA (en) | 2018-12-18 | 2021-07-29 | Inbiose Nv | PRODUCTION OF 3-FUCOSYLLACTOSE AND LACTOSE CONVERTING a-1,3-FUCOSYLTRANSFERASE ENZYMES |
EP3702468A1 (en) * | 2019-03-01 | 2020-09-02 | Jennewein Biotechnologie GmbH | Fermentative production of carbohydrates by microbial cells utilizing a mixed feedstock |
CN110241062A (en) * | 2019-06-27 | 2019-09-17 | 江南大学 | New escherichia expression system |
CA3147502A1 (en) | 2019-07-19 | 2021-01-28 | Inbiose N.V. | Production of fucosyllactose in host cells |
CN110452845B (en) * | 2019-08-15 | 2021-03-02 | 江南大学 | Escherichia coli for producing sucrose phosphorylase |
CN110527704B (en) * | 2019-10-31 | 2020-03-13 | 烟台华康荣赞生物科技有限公司 | Method for synthesizing lacto-N-disaccharide |
EP4077648A1 (en) | 2019-12-17 | 2022-10-26 | Inbiose N.V. | Lactose converting alpha-1,2-fucosyltransferase enzymes |
WO2021123113A1 (en) | 2019-12-18 | 2021-06-24 | Inbiose N.V. | Production of sialylated oligosaccharide in host cells |
WO2021160830A1 (en) | 2020-02-14 | 2021-08-19 | Inbiose N.V. | Glycominimized bacterial host cells |
US20220396817A1 (en) | 2020-02-14 | 2022-12-15 | Inbiose N.V. | Production of glycosylated product in host cells |
CN111172174B (en) * | 2020-03-06 | 2022-02-15 | 沈阳农业大学 | Application of OsUGE3 gene in improving rice traits |
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WO2022013143A1 (en) | 2020-07-13 | 2022-01-20 | Glycom A/S | Oligosaccharide production |
CN116171328A (en) | 2020-08-10 | 2023-05-26 | 因比奥斯公司 | Method for producing purified mixtures of different oligosaccharides produced by cell culture or microbial fermentation |
CN116171329A (en) | 2020-08-10 | 2023-05-26 | 因比奥斯公司 | Method for purifying oligosaccharide solutions produced by cell culture or microbial fermentation |
DK3954778T3 (en) | 2020-08-10 | 2023-12-18 | Inbiose Nv | PRODUCTION OF A MIXTURE OF NEUTRAL NON-FUCOSYLATE OLIGOSACCHARIDES BY A CELL |
CN116323930A (en) | 2020-08-10 | 2023-06-23 | 因比奥斯公司 | Cell production of sialylated di-and/or oligosaccharides |
WO2022034077A1 (en) | 2020-08-10 | 2022-02-17 | Inbiose N.V. | Production of alpha-1,3 glycosylated form of fuc-a1,2-gal-r |
TW202221129A (en) | 2020-08-10 | 2022-06-01 | 比利時商因比奧斯公司 | Cellular production of di- and/or oligosaccharides |
EP4200318A4 (en) * | 2020-08-19 | 2024-06-12 | Conagen Inc. | Biosynthetic production of 2-fucosyllactose |
WO2022124302A1 (en) * | 2020-12-08 | 2022-06-16 | 国立大学法人神戸大学 | Method for establishing metabolically engineered microorganism strain capable of high-throughput production of useful compound, and metabolically engineered escherichia coli strain |
WO2022129470A1 (en) | 2020-12-18 | 2022-06-23 | Inbiose N.V. | Variant sucrose permease polypeptides |
CN116783291A (en) | 2021-01-18 | 2023-09-19 | 因比奥斯公司 | Cellular production of glycosylation products |
TW202246496A (en) | 2021-01-20 | 2022-12-01 | 比利時商因比奥斯公司 | Production of oligosaccharides comprising ln3 as core structure in host cells |
WO2022219187A1 (en) | 2021-04-16 | 2022-10-20 | Inbiose N.V. | Cellular production of bioproducts |
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JP2024516207A (en) | 2021-05-17 | 2024-04-12 | ディーエスエム アイピー アセッツ ビー.ブイ. | Microbial strains expressing invertase/sucrose hydrolase |
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CN114645070B (en) * | 2022-02-28 | 2022-12-20 | 北京焉支山科技有限公司 | Preparation method of hexose-6-phosphoric acid composition and application thereof in cosmetics |
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WO2024017987A1 (en) | 2022-07-20 | 2024-01-25 | Inbiose N.V. | Production of oligosaccharides in host cells |
CN116948928B (en) * | 2023-06-02 | 2024-06-18 | 虹摹生物科技(上海)有限公司 | Seed culture medium and fermentation production method of 2' -fucosyllactose without antibiotics and IPTG inducer |
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