SG11202106291RA - PRODUCTION OF 3-FUCOSYLLACTOSE AND LACTOSE CONVERTING a-1,3-FUCOSYLTRANSFERASE ENZYMES - Google Patents

PRODUCTION OF 3-FUCOSYLLACTOSE AND LACTOSE CONVERTING a-1,3-FUCOSYLTRANSFERASE ENZYMES

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Publication number
SG11202106291RA
SG11202106291RA SG11202106291RA SG11202106291RA SG11202106291RA SG 11202106291R A SG11202106291R A SG 11202106291RA SG 11202106291R A SG11202106291R A SG 11202106291RA SG 11202106291R A SG11202106291R A SG 11202106291RA SG 11202106291R A SG11202106291R A SG 11202106291RA
Authority
SG
Singapore
Prior art keywords
fucosyllactose
lactose
converting
production
fucosyltransferase enzymes
Prior art date
Application number
SG11202106291RA
Inventor
Joeri Beauprez
Nausicaä Lannoo
Kristof Vandewalle
Annelies Vercauteren
Original Assignee
Inbiose Nv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Inbiose Nv filed Critical Inbiose Nv
Publication of SG11202106291RA publication Critical patent/SG11202106291RA/en

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/18Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00Processes for the preparation of sugar derivatives
    • C07H1/06Separation; Purification
    • C07H1/08Separation; Purification from natural products
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • C12N1/16Yeasts; Culture media therefor
    • C12N1/18Baker's yeast; Brewer's yeast
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70Vectors or expression systems specially adapted for E. coli
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1048Glycosyltransferases (2.4)
    • C12N9/1051Hexosyltransferases (2.4.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y204/00Glycosyltransferases (2.4)
    • C12Y204/01Hexosyltransferases (2.4.1)
    • C12Y204/010653-Galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase (2.4.1.65), i.e. alpha-1-3 fucosyltransferase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y204/00Glycosyltransferases (2.4)
    • C12Y204/01Hexosyltransferases (2.4.1)
    • C12Y204/011524-Galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase (2.4.1.152)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Botany (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
SG11202106291RA 2018-12-18 2019-12-18 PRODUCTION OF 3-FUCOSYLLACTOSE AND LACTOSE CONVERTING a-1,3-FUCOSYLTRANSFERASE ENZYMES SG11202106291RA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP18213728 2018-12-18
PCT/EP2019/085841 WO2020127417A2 (en) 2018-12-18 2019-12-18 PRODUCTION OF 3-FUCOSYLLACTOSE AND LACTOSE CONVERTING α-1,3-FUCOSYLTRANSFERASE ENZYMES

Publications (1)

Publication Number Publication Date
SG11202106291RA true SG11202106291RA (en) 2021-07-29

Family

ID=64745987

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202106291RA SG11202106291RA (en) 2018-12-18 2019-12-18 PRODUCTION OF 3-FUCOSYLLACTOSE AND LACTOSE CONVERTING a-1,3-FUCOSYLTRANSFERASE ENZYMES

Country Status (10)

Country Link
US (1) US20220002773A1 (en)
EP (1) EP3898642A2 (en)
JP (1) JP2022514743A (en)
KR (1) KR20210107736A (en)
CN (1) CN113195509A (en)
AU (1) AU2019409833A1 (en)
BR (1) BR112021011298A2 (en)
CA (1) CA3121848A1 (en)
SG (1) SG11202106291RA (en)
WO (1) WO2020127417A2 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114438001B (en) * 2020-11-05 2022-11-15 中国科学院上海高等研究院 Recombinant bacillus subtilis for producing 3-fucosyllactose and construction method and application thereof
CN112662604B (en) * 2020-12-29 2023-10-20 量子高科(广东)生物有限公司 Recombinant escherichia coli for synthesizing 3-fucosyllactose and construction method thereof
CN113648405B (en) * 2021-08-19 2023-06-02 重庆医科大学 Oral recombinant helicobacter pylori protein vaccine nanoparticle and preparation method thereof
WO2023110994A1 (en) * 2021-12-14 2023-06-22 Inbiose N.V. Production of alpha-1,4-fucosylated compounds
WO2023110995A1 (en) * 2021-12-14 2023-06-22 Inbiose N.V. Production of alpha-1,3-fucosylated compounds
CN115287273A (en) * 2022-06-30 2022-11-04 华熙生物科技股份有限公司 1, 2-fucosyltransferase and fusion protein and encoding gene thereof
CN115058465A (en) * 2022-06-30 2022-09-16 山东大学 Fucosylated chondroitin and preparation method and application thereof
KR20240017427A (en) * 2022-07-29 2024-02-08 주식회사 진켐 Recombinant microorganism for producing 3'-fucosyllactose and methods using the same
WO2024089131A1 (en) 2022-10-25 2024-05-02 Inbiose N.V. Saccharide importers for lacto-n-triose
CN116948928B (en) * 2023-06-02 2024-06-18 虹摹生物科技(上海)有限公司 Seed culture medium and fermentation production method of 2' -fucosyllactose without antibiotics and IPTG inducer
CN116425810B (en) * 2023-06-14 2023-08-11 山东合成远景生物科技有限公司 Purification method of 3-fucosyllactose in mixed solution
CN118047820A (en) * 2024-04-16 2024-05-17 北京三元食品股份有限公司 Preparation method of 3-fucosyllactose and prepared standard substance

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU8005098A (en) 1997-06-06 1998-12-21 Governors Of The University Of Alberta, The Alpha1,3-fucosyltransferase of helicobacter pylori
EP3235907B1 (en) * 2010-07-12 2023-06-21 Inbiose N.V. Metabolically engineered organisms for the production of added value bio-products
ES2663627T3 (en) * 2010-10-11 2018-04-16 Jennewein Biotechnologie Gmbh Novel fucosyltransferases and their applications
WO2016040531A1 (en) * 2014-09-09 2016-03-17 Glycosyn LLC Alpha (1,3) fucosyltransferases for use in the production of fucosylated oligosaccharides
RU2021109889A (en) 2014-11-14 2021-05-06 Инбиос Н.В. MUTANT MICROORGANISMS RESISTANT TO DEATH UNDER THE ACTION OF LACTOSE
CN108103039B (en) * 2016-11-25 2021-10-22 上海交通大学 Fucosyltransferase mutants and screening method and application thereof

Also Published As

Publication number Publication date
US20220002773A1 (en) 2022-01-06
KR20210107736A (en) 2021-09-01
WO2020127417A2 (en) 2020-06-25
WO2020127417A3 (en) 2020-07-30
EP3898642A2 (en) 2021-10-27
AU2019409833A1 (en) 2021-08-05
CN113195509A (en) 2021-07-30
BR112021011298A2 (en) 2021-11-30
JP2022514743A (en) 2022-02-15
CA3121848A1 (en) 2020-06-25

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