CN1845990B - 耐热化蔗糖磷酸化酶(sp)的方法 - Google Patents

耐热化蔗糖磷酸化酶(sp)的方法 Download PDF

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Publication number
CN1845990B
CN1845990B CN2004800253207A CN200480025320A CN1845990B CN 1845990 B CN1845990 B CN 1845990B CN 2004800253207 A CN2004800253207 A CN 2004800253207A CN 200480025320 A CN200480025320 A CN 200480025320A CN 1845990 B CN1845990 B CN 1845990B
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amino acid
sucrose phosphorylase
seq
position corresponding
thermostable
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CN1845990A (zh
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藤井和俊
饭干雅惠
柳濑美千代
高田洋树
鹰羽武史
栗木隆
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Ezaki Glico Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)

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  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN2004800253207A 2003-09-04 2004-08-31 耐热化蔗糖磷酸化酶(sp)的方法 Expired - Fee Related CN1845990B (zh)

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JP2003313305 2003-09-04
JP313305/2003 2003-09-04
PCT/JP2004/012533 WO2005024008A1 (ja) 2003-09-04 2004-08-31 スクロースホスホリラーゼ（ｓｐ）の耐熱化方法

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CN1845990A CN1845990A (zh) 2006-10-11
CN1845990B true CN1845990B (zh) 2012-03-21

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US (1) US7968309B2 (https=)
EP (1) EP1661985B1 (https=)
JP (2) JP4450392B2 (https=)
CN (1) CN1845990B (https=)
AU (1) AU2004271018B2 (https=)
DK (1) DK1661985T3 (https=)
WO (1) WO2005024008A1 (https=)

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WO2010146875A1 (ja) 2009-06-18 2010-12-23 オーミケンシ株式会社 ヨウ素およびアミロースを含有する繊維、その製造法およびその利用
JP5955314B2 (ja) * 2010-04-06 2016-07-20 ウニベルズィタイト・ヘント 熱安定性スクロースホスホリラーゼ
SG186836A1 (en) 2010-07-12 2013-02-28 Univ Gent Metabolically engineered organisms for the production of added value bio-products
CN102021152B (zh) * 2010-10-14 2012-01-11 广西大学 蔗糖磷酸化酶的突变体及其应用
CN107109450A (zh) * 2014-12-23 2017-08-29 纳幕尔杜邦公司 酶促产生的纤维素
KR20170008367A (ko) * 2015-07-13 2017-01-24 씨제이제일제당 (주) 글루코스 전이 활성을 가지는 단백질 및 이의 용도
CN105779404B (zh) * 2016-05-11 2019-04-16 广西大学 一种蔗糖磷酸化酶的突变体t155s及其应用
TWI871271B (zh) * 2016-11-28 2025-02-01 德商Ｃ 樂克塔股份有限公司 海藻糖磷酸化酶
CN107236696B (zh) * 2017-07-31 2019-11-26 江南大学 一种表达L.mesenteroides来源的蔗糖磷酸化酶重组枯草芽孢杆菌
CN112004939A (zh) * 2018-04-23 2020-11-27 丹尼斯科美国公司 用磷酸化酶合成包含β-1,3糖苷键的葡聚糖
EP3827090A1 (en) 2018-07-23 2021-06-02 Danisco US Inc. Alpha-glucose-1 -phosphate synthesis from sucrose and glucan synthesis using glucan phosphorylases
CN109694892B (zh) * 2018-11-27 2021-03-23 北京工商大学 制备红景天苷的方法和试剂盒
CN109988778B (zh) * 2019-05-14 2020-10-27 南京工业大学 一种蔗糖磷酸化酶基因及其应用
WO2021003132A1 (en) * 2019-07-02 2021-01-07 Codexis, Inc. Engineered sucrose phosphorylase variant enzymes
CN110734899B (zh) * 2019-10-31 2021-06-18 江南大学 一种酶活提高的蔗糖磷酸化酶突变体及其构建方法和应用
CN112300977B (zh) * 2020-11-18 2024-01-12 江南大学 合成α-熊果苷的基因工程菌株及其构建方法与应用
CN112375724B (zh) * 2020-11-18 2022-12-16 江南大学 高效合成α-熊果苷的基因工程菌及其构建方法与应用
JP7650668B2 (ja) * 2021-01-12 2025-03-25 第一工業製薬株式会社 セロオリゴ糖の製造方法
WO2023236204A1 (en) * 2022-06-10 2023-12-14 Beren Therapeutics P.B.C. Methods for producing beta-cyclodextrins
CN115044568B (zh) * 2022-06-20 2024-01-23 华熙生物科技股份有限公司 高稳定型的蔗糖磷酸化酶突变体及其应用
CN116144625A (zh) * 2023-02-24 2023-05-23 杭州佳嘉乐生物技术有限公司 重组蔗糖磷酸化酶及其催化糖基化反应的方法
CN116731997A (zh) * 2023-06-19 2023-09-12 暨南大学 一种定点突变的蔗糖磷酸化酶及其应用
CN116987684B (zh) * 2023-08-08 2024-04-26 浙江赞源生物技术有限公司 蔗糖磷酸化酶突变体及其在制备甘油葡糖苷中的应用
CN119842654B (zh) * 2024-12-31 2025-08-05 南京诺云生物科技有限公司 一种耐热性增强的蔗糖磷酸化酶及其在高温催化制备α-熊果苷中的应用
CN120230733B (zh) * 2025-06-03 2025-07-29 山东三元生物科技股份有限公司 一种基于Spytag/SpyCatcher环化修饰的蔗糖磷酸化酶突变体及其应用
CN120230734B (zh) * 2025-06-03 2025-07-29 山东三元生物科技股份有限公司 蔗糖磷酸化酶突变体及其在合成2-O-α-D-吡喃葡萄糖基-L-抗坏血酸中的应用
CN121320295B (zh) * 2025-12-16 2026-03-24 山东三元生物科技股份有限公司 一种蔗糖磷酸化酶突变体及其在α-熊果苷合成中的应用

Citations (1)

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CN1180380A (zh) * 1995-02-10 1998-04-29 孟山都公司 植物中蔗糖磷酸化酶的表达

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GB0107658D0 (en) * 2001-03-27 2001-05-16 Chiron Spa Streptococcus pneumoniae
KR100995248B1 (ko) * 2001-05-28 2010-11-18 에자끼구리고가부시키가이샤 글루칸의 생성방법 및 제조방법

Patent Citations (1)

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Publication number Priority date Publication date Assignee Title
CN1180380A (zh) * 1995-02-10 1998-04-29 孟山都公司 植物中蔗糖磷酸化酶的表达

Non-Patent Citations (2)

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Title
刘华珍.细菌产生的蔗糖磷酸化酶的筛选及其特性.国外医药抗生素分册18 5.1997,18(5),347-348.
刘华珍.细菌产生的蔗糖磷酸化酶的筛选及其特性.国外医药抗生素分册18 5.1997,18(5),347-348. *

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JP2009195255A (ja) 2009-09-03
CN1845990A (zh) 2006-10-11
EP1661985A1 (en) 2006-05-31
AU2004271018A1 (en) 2005-03-17
JPWO2005024008A1 (ja) 2007-11-08
JP4450392B2 (ja) 2010-04-14
EP1661985A4 (en) 2006-12-20
DK1661985T3 (en) 2018-01-08
US7968309B2 (en) 2011-06-28
HK1090668A1 (en) 2006-12-29
US20080206822A1 (en) 2008-08-28
WO2005024008A1 (ja) 2005-03-17
AU2004271018B2 (en) 2007-10-11
EP1661985B1 (en) 2017-09-27

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