CN1845990B - 耐热化蔗糖磷酸化酶(sp)的方法 - Google Patents

耐热化蔗糖磷酸化酶(sp)的方法 Download PDF

Info

Publication number
CN1845990B
CN1845990B CN2004800253207A CN200480025320A CN1845990B CN 1845990 B CN1845990 B CN 1845990B CN 2004800253207 A CN2004800253207 A CN 2004800253207A CN 200480025320 A CN200480025320 A CN 200480025320A CN 1845990 B CN1845990 B CN 1845990B
Authority
CN
China
Prior art keywords
amino acid
sucrose phosphorylase
seq
position corresponding
thermostable
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN2004800253207A
Other languages
English (en)
Chinese (zh)
Other versions
CN1845990A (zh
Inventor
藤井和俊
饭干雅惠
柳濑美千代
高田洋树
鹰羽武史
栗木隆
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ezaki Glico Co Ltd
Original Assignee
Ezaki Glico Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ezaki Glico Co Ltd filed Critical Ezaki Glico Co Ltd
Publication of CN1845990A publication Critical patent/CN1845990A/zh
Application granted granted Critical
Publication of CN1845990B publication Critical patent/CN1845990B/zh
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN2004800253207A 2003-09-04 2004-08-31 耐热化蔗糖磷酸化酶(sp)的方法 Expired - Fee Related CN1845990B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP313305/2003 2003-09-04
JP2003313305 2003-09-04
PCT/JP2004/012533 WO2005024008A1 (ja) 2003-09-04 2004-08-31 スクロースホスホリラーゼ（ｓｐ）の耐熱化方法

Publications (2)

Publication Number Publication Date
CN1845990A CN1845990A (zh) 2006-10-11
CN1845990B true CN1845990B (zh) 2012-03-21

Family

ID=34269765

Family Applications (1)

Application Number Title Priority Date Filing Date
CN2004800253207A Expired - Fee Related CN1845990B (zh) 2003-09-04 2004-08-31 耐热化蔗糖磷酸化酶(sp)的方法

Country Status (7)

Country Link
US (1) US7968309B2 (https=)
EP (1) EP1661985B1 (https=)
JP (2) JP4450392B2 (https=)
CN (1) CN1845990B (https=)
AU (1) AU2004271018B2 (https=)
DK (1) DK1661985T3 (https=)
WO (1) WO2005024008A1 (https=)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102803588B (zh) 2009-06-18 2014-10-08 近江绢丝(股份)有限公司 含碘以及直链淀粉的纤维、其制造方法及其应用
US20130029384A1 (en) 2010-04-06 2013-01-31 Universiteit Gent Thermostable sucrose phosphorylase
SG10201504733XA (en) * 2010-07-12 2015-07-30 Univ Gent Metabolically engineered organisms for the production of added value bio-products
CN102021152B (zh) * 2010-10-14 2012-01-11 广西大学 蔗糖磷酸化酶的突变体及其应用
CN108064306B (zh) * 2014-12-23 2022-11-01 营养与生物科学美国4公司 酶促产生的纤维素
KR20170008367A (ko) * 2015-07-13 2017-01-24 씨제이제일제당 (주) 글루코스 전이 활성을 가지는 단백질 및 이의 용도
CN105779404B (zh) * 2016-05-11 2019-04-16 广西大学 一种蔗糖磷酸化酶的突变体t155s及其应用
PT3545084T (pt) * 2016-11-28 2025-11-17 New Matterhorn Llc Trealose fosforilase
CN107236696B (zh) * 2017-07-31 2019-11-26 江南大学 一种表达L.mesenteroides来源的蔗糖磷酸化酶重组枯草芽孢杆菌
JP7431172B2 (ja) 2018-04-23 2024-02-14 ダニスコ・ユーエス・インク ホスホリラーゼ酵素を用いたβ－１，３グリコシド結合を含むグルカンの合成
US11802275B2 (en) 2018-07-23 2023-10-31 Danisco Us Inc. Alpha-glucose-1-phosphate synthesis from sucrose and glucan synthesis using glucan phosphorylases
CN109694892B (zh) * 2018-11-27 2021-03-23 北京工商大学 制备红景天苷的方法和试剂盒
CN109988778B (zh) * 2019-05-14 2020-10-27 南京工业大学 一种蔗糖磷酸化酶基因及其应用
WO2021003132A1 (en) * 2019-07-02 2021-01-07 Codexis, Inc. Engineered sucrose phosphorylase variant enzymes
CN110734899B (zh) * 2019-10-31 2021-06-18 江南大学 一种酶活提高的蔗糖磷酸化酶突变体及其构建方法和应用
CN112375724B (zh) * 2020-11-18 2022-12-16 江南大学 高效合成α-熊果苷的基因工程菌及其构建方法与应用
CN112300977B (zh) * 2020-11-18 2024-01-12 江南大学 合成α-熊果苷的基因工程菌株及其构建方法与应用
JP7650668B2 (ja) * 2021-01-12 2025-03-25 第一工業製薬株式会社 セロオリゴ糖の製造方法
WO2023236204A1 (en) * 2022-06-10 2023-12-14 Beren Therapeutics P.B.C. Methods for producing beta-cyclodextrins
CN115044568B (zh) * 2022-06-20 2024-01-23 华熙生物科技股份有限公司 高稳定型的蔗糖磷酸化酶突变体及其应用
CN116144625A (zh) * 2023-02-24 2023-05-23 杭州佳嘉乐生物技术有限公司 重组蔗糖磷酸化酶及其催化糖基化反应的方法
CN116731997A (zh) * 2023-06-19 2023-09-12 暨南大学 一种定点突变的蔗糖磷酸化酶及其应用
CN116987684B (zh) * 2023-08-08 2024-04-26 浙江赞源生物技术有限公司 蔗糖磷酸化酶突变体及其在制备甘油葡糖苷中的应用
CN119842654B (zh) * 2024-12-31 2025-08-05 南京诺云生物科技有限公司 一种耐热性增强的蔗糖磷酸化酶及其在高温催化制备α-熊果苷中的应用
CN120230733B (zh) * 2025-06-03 2025-07-29 山东三元生物科技股份有限公司 一种基于Spytag/SpyCatcher环化修饰的蔗糖磷酸化酶突变体及其应用
CN120230734B (zh) * 2025-06-03 2025-07-29 山东三元生物科技股份有限公司 蔗糖磷酸化酶突变体及其在合成2-O-α-D-吡喃葡萄糖基-L-抗坏血酸中的应用
CN121320295B (zh) * 2025-12-16 2026-03-24 山东三元生物科技股份有限公司 一种蔗糖磷酸化酶突变体及其在α-熊果苷合成中的应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1180380A (zh) * 1995-02-10 1998-04-29 孟山都公司 植物中蔗糖磷酸化酶的表达

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0107658D0 (en) * 2001-03-27 2001-05-16 Chiron Spa Streptococcus pneumoniae
AU2002307701C1 (en) * 2001-05-28 2008-04-03 Ezaki Glico Co., Ltd. Production method and preparation method of glucans

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1180380A (zh) * 1995-02-10 1998-04-29 孟山都公司 植物中蔗糖磷酸化酶的表达

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
刘华珍.细菌产生的蔗糖磷酸化酶的筛选及其特性.国外医药抗生素分册18 5.1997,18(5),347-348.
刘华珍.细菌产生的蔗糖磷酸化酶的筛选及其特性.国外医药抗生素分册18 5.1997,18(5),347-348. *

Also Published As

Publication number Publication date
JPWO2005024008A1 (ja) 2007-11-08
AU2004271018B2 (en) 2007-10-11
CN1845990A (zh) 2006-10-11
US7968309B2 (en) 2011-06-28
EP1661985B1 (en) 2017-09-27
EP1661985A1 (en) 2006-05-31
HK1090668A1 (en) 2006-12-29
WO2005024008A1 (ja) 2005-03-17
DK1661985T3 (en) 2018-01-08
JP4450392B2 (ja) 2010-04-14
US20080206822A1 (en) 2008-08-28
EP1661985A4 (en) 2006-12-20
JP2009195255A (ja) 2009-09-03
AU2004271018A1 (en) 2005-03-17

Similar Documents

Publication Publication Date Title
CN1845990B (zh) 耐热化蔗糖磷酸化酶(sp)的方法
US7723090B2 (en) Method of heat-stabilizing α-glucan phosphorylase (GP)
CN101198703B (zh) 生产糖原的方法
WO2000001796A2 (en) Starch debranching enzymes
US7374922B2 (en) Starch debranching enzymes
CN101657544A (zh) 新型α-半乳糖苷酶
KR101627921B1 (ko) 알돌레이즈 돌연변이체 및 이를 이용한 타가토스 생산 방법
JP2004313074A (ja) 新規α−１，２−マンノシダーゼおよびその遺伝子、ならびに該酵素を用いたα−マンノシル糖化合物の製造方法
Mizanur et al. Recombinant production and biochemical characterization of a hyperthermostable α‐glucan/maltodextrin phosphorylase from Pyrococcus furiosus
CN116103353B (zh) 一种纤维二糖磷酸化酶多肽的应用
CN108368492A (zh) 包含使用乙酰微小杆菌和凝结芽孢杆菌α-葡聚糖转移酶的组合物和方法
HK1090668B (en) Method of making sucrose phosphorylase(sp) heat-stable
KR100370882B1 (ko) 서머스 칼도필러스 지케이24 균주 유래 재조합 효소 및이를 이용한 알파-1,4-아밀로오스 제조방법
JPH10150986A (ja) 超耐熱性４−α−グルカノトランスフェラーゼ
KR101142950B1 (ko) 써모코커스 마리너스 균주 유래의 내열성 ｄｎａ 중합효소 및 이의 이용
KR102312806B1 (ko) 비피도박테리움 속 유래인 저항전분 분해 활성을 나타내는 아밀라아제들 및 이의 용도
KR102312807B1 (ko) 비피도박테리움 속의 저항전분 분해 활성을 가지는 아밀라아제들 및 이의 용도
KR20100107873A (ko) 써모코커스 씰러 균주 유래의 내열성 ｄｎａ 중합효소 및 이의 이용
KR20110103750A (ko) 써모코커스 래디오톨러런스 균주 유래의 내열성 ｄｎａ 중합효소 및 이의 이용

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
REG Reference to a national code

Ref country code: HK

Ref legal event code: DE

Ref document number: 1090668

Country of ref document: HK

C14 Grant of patent or utility model
GR01 Patent grant
REG Reference to a national code

Ref country code: HK

Ref legal event code: GR

Ref document number: 1090668

Country of ref document: HK

CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20120321

CF01 Termination of patent right due to non-payment of annual fee