DK1661985T3 - PROCEDURE FOR PREPARING HEAT STABLE SUCROSE PHOSPHORYLASE (SP) - Google Patents
PROCEDURE FOR PREPARING HEAT STABLE SUCROSE PHOSPHORYLASE (SP) Download PDFInfo
- Publication number
- DK1661985T3 DK1661985T3 DK04772489.3T DK04772489T DK1661985T3 DK 1661985 T3 DK1661985 T3 DK 1661985T3 DK 04772489 T DK04772489 T DK 04772489T DK 1661985 T3 DK1661985 T3 DK 1661985T3
- Authority
- DK
- Denmark
- Prior art keywords
- sucrose phosphorylase
- amino acid
- sequence
- position corresponding
- improved thermostability
- Prior art date
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
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- Biotechnology (AREA)
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- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2003313305 | 2003-09-04 | ||
| PCT/JP2004/012533 WO2005024008A1 (ja) | 2003-09-04 | 2004-08-31 | スクロースホスホリラーゼ（ｓｐ）の耐熱化方法 |
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| DK1661985T3 true DK1661985T3 (en) | 2018-01-08 |
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| DK04772489.3T DK1661985T3 (en) | 2003-09-04 | 2004-08-31 | PROCEDURE FOR PREPARING HEAT STABLE SUCROSE PHOSPHORYLASE (SP) |
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| EP (1) | EP1661985B1 (https=) |
| JP (2) | JP4450392B2 (https=) |
| CN (1) | CN1845990B (https=) |
| AU (1) | AU2004271018B2 (https=) |
| DK (1) | DK1661985T3 (https=) |
| WO (1) | WO2005024008A1 (https=) |
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| WO2010146875A1 (ja) | 2009-06-18 | 2010-12-23 | オーミケンシ株式会社 | ヨウ素およびアミロースを含有する繊維、その製造法およびその利用 |
| JP5955314B2 (ja) * | 2010-04-06 | 2016-07-20 | ウニベルズィタイト・ヘント | 熱安定性スクロースホスホリラーゼ |
| SG186836A1 (en) | 2010-07-12 | 2013-02-28 | Univ Gent | Metabolically engineered organisms for the production of added value bio-products |
| CN102021152B (zh) * | 2010-10-14 | 2012-01-11 | 广西大学 | 蔗糖磷酸化酶的突变体及其应用 |
| CN107109450A (zh) * | 2014-12-23 | 2017-08-29 | 纳幕尔杜邦公司 | 酶促产生的纤维素 |
| KR20170008367A (ko) * | 2015-07-13 | 2017-01-24 | 씨제이제일제당 (주) | 글루코스 전이 활성을 가지는 단백질 및 이의 용도 |
| CN105779404B (zh) * | 2016-05-11 | 2019-04-16 | 广西大学 | 一种蔗糖磷酸化酶的突变体t155s及其应用 |
| TWI871271B (zh) * | 2016-11-28 | 2025-02-01 | 德商Ｃ 樂克塔股份有限公司 | 海藻糖磷酸化酶 |
| CN107236696B (zh) * | 2017-07-31 | 2019-11-26 | 江南大学 | 一种表达L.mesenteroides来源的蔗糖磷酸化酶重组枯草芽孢杆菌 |
| CN112004939A (zh) * | 2018-04-23 | 2020-11-27 | 丹尼斯科美国公司 | 用磷酸化酶合成包含β-1,3糖苷键的葡聚糖 |
| EP3827090A1 (en) | 2018-07-23 | 2021-06-02 | Danisco US Inc. | Alpha-glucose-1 -phosphate synthesis from sucrose and glucan synthesis using glucan phosphorylases |
| CN109694892B (zh) * | 2018-11-27 | 2021-03-23 | 北京工商大学 | 制备红景天苷的方法和试剂盒 |
| CN109988778B (zh) * | 2019-05-14 | 2020-10-27 | 南京工业大学 | 一种蔗糖磷酸化酶基因及其应用 |
| WO2021003132A1 (en) * | 2019-07-02 | 2021-01-07 | Codexis, Inc. | Engineered sucrose phosphorylase variant enzymes |
| CN110734899B (zh) * | 2019-10-31 | 2021-06-18 | 江南大学 | 一种酶活提高的蔗糖磷酸化酶突变体及其构建方法和应用 |
| CN112300977B (zh) * | 2020-11-18 | 2024-01-12 | 江南大学 | 合成α-熊果苷的基因工程菌株及其构建方法与应用 |
| CN112375724B (zh) * | 2020-11-18 | 2022-12-16 | 江南大学 | 高效合成α-熊果苷的基因工程菌及其构建方法与应用 |
| JP7650668B2 (ja) * | 2021-01-12 | 2025-03-25 | 第一工業製薬株式会社 | セロオリゴ糖の製造方法 |
| WO2023236204A1 (en) * | 2022-06-10 | 2023-12-14 | Beren Therapeutics P.B.C. | Methods for producing beta-cyclodextrins |
| CN115044568B (zh) * | 2022-06-20 | 2024-01-23 | 华熙生物科技股份有限公司 | 高稳定型的蔗糖磷酸化酶突变体及其应用 |
| CN116144625A (zh) * | 2023-02-24 | 2023-05-23 | 杭州佳嘉乐生物技术有限公司 | 重组蔗糖磷酸化酶及其催化糖基化反应的方法 |
| CN116731997A (zh) * | 2023-06-19 | 2023-09-12 | 暨南大学 | 一种定点突变的蔗糖磷酸化酶及其应用 |
| CN116987684B (zh) * | 2023-08-08 | 2024-04-26 | 浙江赞源生物技术有限公司 | 蔗糖磷酸化酶突变体及其在制备甘油葡糖苷中的应用 |
| CN119842654B (zh) * | 2024-12-31 | 2025-08-05 | 南京诺云生物科技有限公司 | 一种耐热性增强的蔗糖磷酸化酶及其在高温催化制备α-熊果苷中的应用 |
| CN120230733B (zh) * | 2025-06-03 | 2025-07-29 | 山东三元生物科技股份有限公司 | 一种基于Spytag/SpyCatcher环化修饰的蔗糖磷酸化酶突变体及其应用 |
| CN120230734B (zh) * | 2025-06-03 | 2025-07-29 | 山东三元生物科技股份有限公司 | 蔗糖磷酸化酶突变体及其在合成2-O-α-D-吡喃葡萄糖基-L-抗坏血酸中的应用 |
| CN121320295B (zh) * | 2025-12-16 | 2026-03-24 | 山东三元生物科技股份有限公司 | 一种蔗糖磷酸化酶突变体及其在α-熊果苷合成中的应用 |
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| CN1180380A (zh) * | 1995-02-10 | 1998-04-29 | 孟山都公司 | 植物中蔗糖磷酸化酶的表达 |
| GB0107658D0 (en) * | 2001-03-27 | 2001-05-16 | Chiron Spa | Streptococcus pneumoniae |
| KR100995248B1 (ko) * | 2001-05-28 | 2010-11-18 | 에자끼구리고가부시키가이샤 | 글루칸의 생성방법 및 제조방법 |
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2004
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Also Published As
| Publication number | Publication date |
|---|---|
| JP2009195255A (ja) | 2009-09-03 |
| CN1845990A (zh) | 2006-10-11 |
| CN1845990B (zh) | 2012-03-21 |
| EP1661985A1 (en) | 2006-05-31 |
| AU2004271018A1 (en) | 2005-03-17 |
| JPWO2005024008A1 (ja) | 2007-11-08 |
| JP4450392B2 (ja) | 2010-04-14 |
| EP1661985A4 (en) | 2006-12-20 |
| US7968309B2 (en) | 2011-06-28 |
| HK1090668A1 (en) | 2006-12-29 |
| US20080206822A1 (en) | 2008-08-28 |
| WO2005024008A1 (ja) | 2005-03-17 |
| AU2004271018B2 (en) | 2007-10-11 |
| EP1661985B1 (en) | 2017-09-27 |
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