JP4450392B2 - スクロースホスホリラーゼ（ｓｐ）の耐熱化方法 - Google Patents

スクロースホスホリラーゼ（ｓｐ）の耐熱化方法 Download PDF

Info

Publication number
JP4450392B2
JP4450392B2 JP2005513633A JP2005513633A JP4450392B2 JP 4450392 B2 JP4450392 B2 JP 4450392B2 JP 2005513633 A JP2005513633 A JP 2005513633A JP 2005513633 A JP2005513633 A JP 2005513633A JP 4450392 B2 JP4450392 B2 JP 4450392B2
Authority
JP
Japan
Prior art keywords
position corresponding
amino acid
sucrose phosphorylase
seq
phosphorylase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
JP2005513633A
Other languages
English (en)
Japanese (ja)
Other versions
JPWO2005024008A1 (ja
Inventor
和俊 藤井
雅恵 飯干
美千代 柳瀬
洋樹 高田
武史 鷹羽
隆 栗木
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ezaki Glico Co Ltd
Original Assignee
Ezaki Glico Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ezaki Glico Co Ltd filed Critical Ezaki Glico Co Ltd
Publication of JPWO2005024008A1 publication Critical patent/JPWO2005024008A1/ja
Application granted granted Critical
Publication of JP4450392B2 publication Critical patent/JP4450392B2/ja
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2005513633A 2003-09-04 2004-08-31 スクロースホスホリラーゼ（ｓｐ）の耐熱化方法 Expired - Lifetime JP4450392B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2003313305 2003-09-04
JP2003313305 2003-09-04
PCT/JP2004/012533 WO2005024008A1 (ja) 2003-09-04 2004-08-31 スクロースホスホリラーゼ（ｓｐ）の耐熱化方法

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2009139486A Division JP2009195255A (ja) 2003-09-04 2009-06-10 スクロースホスホリラーゼ（ｓｐ）の耐熱化方法

Publications (2)

Publication Number Publication Date
JPWO2005024008A1 JPWO2005024008A1 (ja) 2007-11-08
JP4450392B2 true JP4450392B2 (ja) 2010-04-14

Family

ID=34269765

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2005513633A Expired - Lifetime JP4450392B2 (ja) 2003-09-04 2004-08-31 スクロースホスホリラーゼ（ｓｐ）の耐熱化方法
JP2009139486A Pending JP2009195255A (ja) 2003-09-04 2009-06-10 スクロースホスホリラーゼ（ｓｐ）の耐熱化方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2009139486A Pending JP2009195255A (ja) 2003-09-04 2009-06-10 スクロースホスホリラーゼ（ｓｐ）の耐熱化方法

Country Status (7)

Country Link
US (1) US7968309B2 (https=)
EP (1) EP1661985B1 (https=)
JP (2) JP4450392B2 (https=)
CN (1) CN1845990B (https=)
AU (1) AU2004271018B2 (https=)
DK (1) DK1661985T3 (https=)
WO (1) WO2005024008A1 (https=)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010146875A1 (ja) 2009-06-18 2010-12-23 オーミケンシ株式会社 ヨウ素およびアミロースを含有する繊維、その製造法およびその利用
JP5955314B2 (ja) * 2010-04-06 2016-07-20 ウニベルズィタイト・ヘント 熱安定性スクロースホスホリラーゼ
SG186836A1 (en) 2010-07-12 2013-02-28 Univ Gent Metabolically engineered organisms for the production of added value bio-products
CN102021152B (zh) * 2010-10-14 2012-01-11 广西大学 蔗糖磷酸化酶的突变体及其应用
CN107109450A (zh) * 2014-12-23 2017-08-29 纳幕尔杜邦公司 酶促产生的纤维素
KR20170008367A (ko) * 2015-07-13 2017-01-24 씨제이제일제당 (주) 글루코스 전이 활성을 가지는 단백질 및 이의 용도
CN105779404B (zh) * 2016-05-11 2019-04-16 广西大学 一种蔗糖磷酸化酶的突变体t155s及其应用
TWI871271B (zh) * 2016-11-28 2025-02-01 德商Ｃ 樂克塔股份有限公司 海藻糖磷酸化酶
CN107236696B (zh) * 2017-07-31 2019-11-26 江南大学 一种表达L.mesenteroides来源的蔗糖磷酸化酶重组枯草芽孢杆菌
CN112004939A (zh) * 2018-04-23 2020-11-27 丹尼斯科美国公司 用磷酸化酶合成包含β-1,3糖苷键的葡聚糖
EP3827090A1 (en) 2018-07-23 2021-06-02 Danisco US Inc. Alpha-glucose-1 -phosphate synthesis from sucrose and glucan synthesis using glucan phosphorylases
CN109694892B (zh) * 2018-11-27 2021-03-23 北京工商大学 制备红景天苷的方法和试剂盒
CN109988778B (zh) * 2019-05-14 2020-10-27 南京工业大学 一种蔗糖磷酸化酶基因及其应用
WO2021003132A1 (en) * 2019-07-02 2021-01-07 Codexis, Inc. Engineered sucrose phosphorylase variant enzymes
CN110734899B (zh) * 2019-10-31 2021-06-18 江南大学 一种酶活提高的蔗糖磷酸化酶突变体及其构建方法和应用
CN112300977B (zh) * 2020-11-18 2024-01-12 江南大学 合成α-熊果苷的基因工程菌株及其构建方法与应用
CN112375724B (zh) * 2020-11-18 2022-12-16 江南大学 高效合成α-熊果苷的基因工程菌及其构建方法与应用
JP7650668B2 (ja) * 2021-01-12 2025-03-25 第一工業製薬株式会社 セロオリゴ糖の製造方法
WO2023236204A1 (en) * 2022-06-10 2023-12-14 Beren Therapeutics P.B.C. Methods for producing beta-cyclodextrins
CN115044568B (zh) * 2022-06-20 2024-01-23 华熙生物科技股份有限公司 高稳定型的蔗糖磷酸化酶突变体及其应用
CN116144625A (zh) * 2023-02-24 2023-05-23 杭州佳嘉乐生物技术有限公司 重组蔗糖磷酸化酶及其催化糖基化反应的方法
CN116731997A (zh) * 2023-06-19 2023-09-12 暨南大学 一种定点突变的蔗糖磷酸化酶及其应用
CN116987684B (zh) * 2023-08-08 2024-04-26 浙江赞源生物技术有限公司 蔗糖磷酸化酶突变体及其在制备甘油葡糖苷中的应用
CN119842654B (zh) * 2024-12-31 2025-08-05 南京诺云生物科技有限公司 一种耐热性增强的蔗糖磷酸化酶及其在高温催化制备α-熊果苷中的应用
CN120230733B (zh) * 2025-06-03 2025-07-29 山东三元生物科技股份有限公司 一种基于Spytag/SpyCatcher环化修饰的蔗糖磷酸化酶突变体及其应用
CN120230734B (zh) * 2025-06-03 2025-07-29 山东三元生物科技股份有限公司 蔗糖磷酸化酶突变体及其在合成2-O-α-D-吡喃葡萄糖基-L-抗坏血酸中的应用
CN121320295B (zh) * 2025-12-16 2026-03-24 山东三元生物科技股份有限公司 一种蔗糖磷酸化酶突变体及其在α-熊果苷合成中的应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1180380A (zh) * 1995-02-10 1998-04-29 孟山都公司 植物中蔗糖磷酸化酶的表达
GB0107658D0 (en) * 2001-03-27 2001-05-16 Chiron Spa Streptococcus pneumoniae
KR100995248B1 (ko) * 2001-05-28 2010-11-18 에자끼구리고가부시키가이샤 글루칸의 생성방법 및 제조방법

Also Published As

Publication number Publication date
JP2009195255A (ja) 2009-09-03
CN1845990A (zh) 2006-10-11
CN1845990B (zh) 2012-03-21
EP1661985A1 (en) 2006-05-31
AU2004271018A1 (en) 2005-03-17
JPWO2005024008A1 (ja) 2007-11-08
EP1661985A4 (en) 2006-12-20
DK1661985T3 (en) 2018-01-08
US7968309B2 (en) 2011-06-28
HK1090668A1 (en) 2006-12-29
US20080206822A1 (en) 2008-08-28
WO2005024008A1 (ja) 2005-03-17
AU2004271018B2 (en) 2007-10-11
EP1661985B1 (en) 2017-09-27

Similar Documents

Publication Publication Date Title
JP4450392B2 (ja) スクロースホスホリラーゼ（ｓｐ）の耐熱化方法
US7723090B2 (en) Method of heat-stabilizing α-glucan phosphorylase (GP)
AU699552B2 (en) DNA sequences coding for enzymes capable of facilitating the synthesis of linear alpha-1,4 glucans in plants, fungi and microorganisms
Monchois et al. Characterization of Leuconostoc mesenteroides NRRL B-512F dextransucrase (DSRS) and identification of amino-acid residues playing a key role in enzyme activity
US9957488B2 (en) Pharmaceutical compositions and dermatogic compositions synthesized from catalytic domains producing highly α1,2 branched dextrans
HU226813B1 (en) Nucleic acid molecules which encode proteins having fructosyl transferase activity and methods for producing long-chain inulin
JP4086312B2 (ja) グリコーゲンの製造方法
KR20010081985A (ko) 전분 절지 효소
EP2652130B1 (en) Trehalose phosphorylases and their use in the biocatalytic production of trehalose-analogues and glycosyl phosphates
JP2004313074A (ja) 新規α−１，２−マンノシダーゼおよびその遺伝子、ならびに該酵素を用いたα−マンノシル糖化合物の製造方法
Mizanur et al. Recombinant production and biochemical characterization of a hyperthermostable α‐glucan/maltodextrin phosphorylase from Pyrococcus furiosus
US6506592B1 (en) Hyperthermophilic alpha-glucosidase gene and its use
Koo et al. Cloning, sequencing, and expression of UDP-glucose pyrophosphorylase gene from Acetobacter xylinum BRC5
JPH10150986A (ja) 超耐熱性４−α−グルカノトランスフェラーゼ
JP7025941B2 (ja) 新規酵素剤、その製造方法およびその用途
HK1090668B (en) Method of making sucrose phosphorylase(sp) heat-stable
KR100370882B1 (ko) 서머스 칼도필러스 지케이24 균주 유래 재조합 효소 및이를 이용한 알파-1,4-아밀로오스 제조방법
JP4340107B2 (ja) 基質特異性改変キメラ酵素
KR20050051055A (ko) 더머스 칼도필러스 ｇｋ24 균주 유래의 알파-글루칸포스포릴라제, 재조합 균주를 이용한 상기 효소의제조방법 및 상기 효소를 이용한알파--디-포도당-1-인산염의 합성방법
KR101294598B1 (ko) 4-알파-글루카노트랜스퍼레이즈와 아밀로오스의 복합체 및 이의 이용
Berger Cloning and expression of the novel glgC genes from Chlamydia trachomatis and Bacillus subtilis and directed evolution of the recombinant Chlamydia ADP-glucose pyrophosphorylase

Legal Events

Date Code Title Description
A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20090414

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20090610

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20091014

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20091210

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20100121

A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20100122

R150 Certificate of patent or registration of utility model

Ref document number: 4450392

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

Free format text: JAPANESE INTERMEDIATE CODE: R150

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20130205

Year of fee payment: 3

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20130205

Year of fee payment: 3

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20140205

Year of fee payment: 4

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20150205

Year of fee payment: 5

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

RD04 Notification of resignation of power of attorney

Free format text: JAPANESE INTERMEDIATE CODE: R3D04

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

EXPY Cancellation because of completion of term