JP2007537314A - 架橋したゼラチンを基にした造形体の製造法 - Google Patents
架橋したゼラチンを基にした造形体の製造法 Download PDFInfo
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- JP2007537314A JP2007537314A JP2007512103A JP2007512103A JP2007537314A JP 2007537314 A JP2007537314 A JP 2007537314A JP 2007512103 A JP2007512103 A JP 2007512103A JP 2007512103 A JP2007512103 A JP 2007512103A JP 2007537314 A JP2007537314 A JP 2007537314A
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- shaped body
- gelatin
- sheet material
- cross
- crosslinking
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- Polymers & Plastics (AREA)
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- Materials Engineering (AREA)
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- Rheumatology (AREA)
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- Materials For Medical Uses (AREA)
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- Laminated Bodies (AREA)
- Prostheses (AREA)
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- Manufacture Of Porous Articles, And Recovery And Treatment Of Waste Products (AREA)
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Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE102004024635A DE102004024635A1 (de) | 2004-05-12 | 2004-05-12 | Verfahren zur Herstellung von Formkörpern auf Basis von vernetzter Gelatine |
| PCT/EP2005/005174 WO2005111121A2 (de) | 2004-05-12 | 2005-05-12 | Verfahren zur herstellung von formkörpern auf basis von vernetzter gelatine |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2007537314A true JP2007537314A (ja) | 2007-12-20 |
| JP2007537314A5 JP2007537314A5 (https=) | 2008-06-26 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| JP2007512103A Pending JP2007537314A (ja) | 2004-05-12 | 2005-05-12 | 架橋したゼラチンを基にした造形体の製造法 |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US20070077274A1 (https=) |
| EP (1) | EP1747247A2 (https=) |
| JP (1) | JP2007537314A (https=) |
| AU (1) | AU2005243474A1 (https=) |
| BR (1) | BRPI0510782A (https=) |
| CA (1) | CA2558351A1 (https=) |
| CR (1) | CR8427A (https=) |
| DE (1) | DE102004024635A1 (https=) |
| EA (1) | EA200602031A1 (https=) |
| EC (1) | ECSP066989A (https=) |
| NO (1) | NO20065652L (https=) |
| WO (1) | WO2005111121A2 (https=) |
| ZA (1) | ZA200609115B (https=) |
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| JP2011036320A (ja) * | 2009-08-07 | 2011-02-24 | Rie Tsuchiya | 軟骨用移植材 |
| JP2012523870A (ja) * | 2009-04-17 | 2012-10-11 | テテック ティシュー エンジニアリング テクノロジーズ アクチェンゲゼルシャフト | ヒト及び／又は動物の筋骨格系に関連する損傷及び／又は疾患の治療のための移植片及び治療用組成物 |
| JP2014073408A (ja) * | 2008-06-18 | 2014-04-24 | Lifebond Ltd | 改良された架橋組成物 |
| JP5497449B2 (ja) * | 2007-11-28 | 2014-05-21 | 富士フイルム株式会社 | 生体高分子およびポリペプチドの化学修飾方法 |
| JP2015208369A (ja) * | 2014-04-24 | 2015-11-24 | 国立大学法人京都工芸繊維大学 | 機能性架橋構造体及びその製造方法並びに医療材料 |
| JP2017179175A (ja) * | 2016-03-31 | 2017-10-05 | 富士フイルム株式会社 | ゼラチン成形体の製造方法 |
| US10202585B2 (en) | 2009-12-22 | 2019-02-12 | Lifebond Ltd | Modification of enzymatic crosslinkers for controlling properties of crosslinked matrices |
| JP2019146534A (ja) * | 2018-02-28 | 2019-09-05 | 澁谷工業株式会社 | ゼラチン支持体を用いた細胞培養方法 |
| JP2020130541A (ja) * | 2019-02-18 | 2020-08-31 | 青葉化成株式会社 | 医療材料およびその製造方法 |
| US11998654B2 (en) | 2018-07-12 | 2024-06-04 | Bard Shannon Limited | Securing implants and medical devices |
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| DE102005054937A1 (de) * | 2005-11-17 | 2007-05-24 | Gelita Ag | Angiogenese förderndes Substrat |
| US8351679B2 (en) * | 2006-05-23 | 2013-01-08 | Kirin Techno-System Company, Limited | Exclusion of recognized parts from inspection of a cylindrical object |
| DE102006033167A1 (de) * | 2006-07-10 | 2008-01-24 | Gelita Ag | Verwendung von Gelatine und einem Vernetzungsmittel zur Herstellung eines vernetzenden medizinischen Klebers |
| DE102006033168A1 (de) | 2006-07-10 | 2008-01-17 | Gelita Ag | Verwendung von Gelatine und einem Vernetzungsmittel zur Herstellung einer vernetzenden therapeutischen Zusammensetzung |
| GB2444323B (en) * | 2006-11-30 | 2011-04-06 | Ethicon Inc | Protein sheet material |
| JPWO2008072379A1 (ja) * | 2006-12-13 | 2010-03-25 | 富士フイルム株式会社 | 修飾された生体高分子の製造方法及び生体高分子の架橋方法 |
| DE102007024256A1 (de) * | 2007-05-16 | 2008-11-20 | Gelita Ag | Gefäßstent |
| DE102007024239A1 (de) * | 2007-05-16 | 2008-11-20 | Gelita Ag | Angiogenese förderndes Substrat |
| DE102007051059B4 (de) | 2007-10-18 | 2014-04-03 | NMI Naturwissenschaftliches und Medizinisches Institut an der Universität Tübingen | Bioverbundmaterial für die kontrollierte Freisetzung von Wirkstoffen |
| DE102008020197A1 (de) | 2008-04-15 | 2009-10-22 | Gelita Ag | Schnell benetzbares, Hydrokolloid enthaltendes Material, Verfahren zu dessen Herstellung sowie dessen Verwendung |
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| US9849217B2 (en) | 2013-04-18 | 2017-12-26 | Board Of Regents, The University Of Texas System | Antimicrobial wraps for medical implants |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS62257387A (ja) * | 1986-04-18 | 1987-11-09 | アンステイチユ メリユ | コラ−ゲンシ−トの製造方法、該製造方法により得られるコラ−ゲンシ−トおよび該コラ−ゲンシ−トの応用 |
| JPH04135483A (ja) * | 1990-09-27 | 1992-05-08 | Kanebo Ltd | 二層性ゼラチンシート及びその製造方法 |
| JPH0654899A (ja) * | 1991-05-02 | 1994-03-01 | Kanebo Ltd | 二層性蛋白質シ−トの製造方法 |
| JP2004065780A (ja) * | 2002-08-08 | 2004-03-04 | Gunze Ltd | 生体材料およびそれを用いた癒着防止材 |
Family Cites Families (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB191329509A (en) * | 1913-12-22 | 1914-07-09 | Thomas Steele | Improved Method of Manufacturing Artificial Tooth Fronts. |
| DE7135934U (de) * | 1971-09-22 | 1971-12-16 | Agfa Gevaert Ag | Optische filter |
| DE2218009A1 (de) * | 1972-04-14 | 1973-11-08 | Agfa Gevaert Ag | Verfahren zur haertung gelatinehaltiger schichten |
| GB1538075A (en) * | 1975-03-24 | 1979-01-10 | Champion Paper Co Ltd | Formation of microcapsules by interfacial cross-linking and microcapsules produced thereby |
| US4264493A (en) * | 1978-10-18 | 1981-04-28 | Battista Orlando A | Natural protein polymer hydrogels |
| US4787900A (en) * | 1982-04-19 | 1988-11-29 | Massachusetts Institute Of Technology | Process for forming multilayer bioreplaceable blood vessel prosthesis |
| DE3608158A1 (de) * | 1986-03-12 | 1987-09-17 | Braun Melsungen Ag | Mit vernetzter gelatine impraegnierte gefaessprothese und verfahren zu ihrer herstellung |
| US5863531A (en) * | 1986-04-18 | 1999-01-26 | Advanced Tissue Sciences, Inc. | In vitro preparation of tubular tissue structures by stromal cell culture on a three-dimensional framework |
| US4904554A (en) * | 1988-03-21 | 1990-02-27 | Hughes Aircraft Company | Gas phase hardening of gelatin holograms |
| US5206023A (en) * | 1991-01-31 | 1993-04-27 | Robert F. Shaw | Method and compositions for the treatment and repair of defects or lesions in cartilage |
| JP2699260B2 (ja) * | 1994-05-18 | 1998-01-19 | 和歌山県 | ゼラチン樹脂の製造方法 |
| US5518732A (en) * | 1995-02-14 | 1996-05-21 | Chiron Vision, Inc. | Bio-erodible ophthalmic shield |
| US5834232A (en) * | 1996-05-01 | 1998-11-10 | Zymogenetics, Inc. | Cross-linked gelatin gels and methods of making them |
| TW528600B (en) * | 1996-11-20 | 2003-04-21 | Yasuhiko Shimizu | Artificial neural canal |
| DE19922078A1 (de) * | 1999-05-15 | 2000-11-23 | Weitzel Kage Doris | Gewebekonstrukt für die Transplantationschirurgie |
| JP4275836B2 (ja) * | 2000-02-24 | 2009-06-10 | 富士フイルム株式会社 | 水可溶性鎖延長ゼラチンの製造方法、それにより製造されたゼラチン、及び該ゼラチンを含むハロゲン化銀写真感光材料 |
| US7029838B2 (en) * | 2001-03-30 | 2006-04-18 | Arizona Board Of Regents On Behalf Of The University Of Arizona | Prevascularized contructs for implantation to provide blood perfusion |
| DE10135275A1 (de) * | 2001-07-13 | 2003-01-30 | Jotec Gmbh | Implantat und Verfahren zu seiner Herstellung |
| DE10149392A1 (de) * | 2001-09-27 | 2003-04-24 | Aesculap Ag & Co Kg | Implantat, insbesondere Gefäßprothese, und Verfahren zu seiner Herstellung |
-
2004
- 2004-05-12 DE DE102004024635A patent/DE102004024635A1/de not_active Withdrawn
-
2005
- 2005-05-12 AU AU2005243474A patent/AU2005243474A1/en not_active Abandoned
- 2005-05-12 WO PCT/EP2005/005174 patent/WO2005111121A2/de not_active Ceased
- 2005-05-12 CA CA002558351A patent/CA2558351A1/en not_active Abandoned
- 2005-05-12 BR BRPI0510782-2A patent/BRPI0510782A/pt not_active IP Right Cessation
- 2005-05-12 EA EA200602031A patent/EA200602031A1/ru unknown
- 2005-05-12 JP JP2007512103A patent/JP2007537314A/ja active Pending
- 2005-05-12 EP EP05745724A patent/EP1747247A2/de not_active Withdrawn
-
2006
- 2006-06-01 CR CR8427A patent/CR8427A/es not_active Application Discontinuation
- 2006-11-01 US US11/555,295 patent/US20070077274A1/en not_active Abandoned
- 2006-11-01 ZA ZA200609115A patent/ZA200609115B/en unknown
- 2006-11-10 EC EC2006006989A patent/ECSP066989A/es unknown
- 2006-12-07 NO NO20065652A patent/NO20065652L/no not_active Application Discontinuation
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS62257387A (ja) * | 1986-04-18 | 1987-11-09 | アンステイチユ メリユ | コラ−ゲンシ−トの製造方法、該製造方法により得られるコラ−ゲンシ−トおよび該コラ−ゲンシ−トの応用 |
| JPH04135483A (ja) * | 1990-09-27 | 1992-05-08 | Kanebo Ltd | 二層性ゼラチンシート及びその製造方法 |
| JPH0654899A (ja) * | 1991-05-02 | 1994-03-01 | Kanebo Ltd | 二層性蛋白質シ−トの製造方法 |
| JP2004065780A (ja) * | 2002-08-08 | 2004-03-04 | Gunze Ltd | 生体材料およびそれを用いた癒着防止材 |
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5497449B2 (ja) * | 2007-11-28 | 2014-05-21 | 富士フイルム株式会社 | 生体高分子およびポリペプチドの化学修飾方法 |
| JP2014073408A (ja) * | 2008-06-18 | 2014-04-24 | Lifebond Ltd | 改良された架橋組成物 |
| JP2012523870A (ja) * | 2009-04-17 | 2012-10-11 | テテック ティシュー エンジニアリング テクノロジーズ アクチェンゲゼルシャフト | ヒト及び／又は動物の筋骨格系に関連する損傷及び／又は疾患の治療のための移植片及び治療用組成物 |
| JP2011036320A (ja) * | 2009-08-07 | 2011-02-24 | Rie Tsuchiya | 軟骨用移植材 |
| US10202585B2 (en) | 2009-12-22 | 2019-02-12 | Lifebond Ltd | Modification of enzymatic crosslinkers for controlling properties of crosslinked matrices |
| JP2015208369A (ja) * | 2014-04-24 | 2015-11-24 | 国立大学法人京都工芸繊維大学 | 機能性架橋構造体及びその製造方法並びに医療材料 |
| JP2017179175A (ja) * | 2016-03-31 | 2017-10-05 | 富士フイルム株式会社 | ゼラチン成形体の製造方法 |
| JP2019146534A (ja) * | 2018-02-28 | 2019-09-05 | 澁谷工業株式会社 | ゼラチン支持体を用いた細胞培養方法 |
| JP7041346B2 (ja) | 2018-02-28 | 2022-03-24 | 澁谷工業株式会社 | ゼラチン支持体を用いた細胞培養方法 |
| US11998654B2 (en) | 2018-07-12 | 2024-06-04 | Bard Shannon Limited | Securing implants and medical devices |
| JP2020130541A (ja) * | 2019-02-18 | 2020-08-31 | 青葉化成株式会社 | 医療材料およびその製造方法 |
| JP7303511B2 (ja) | 2019-02-18 | 2023-07-05 | 青葉化成株式会社 | 医療材料およびその製造方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| CA2558351A1 (en) | 2005-11-24 |
| WO2005111121A2 (de) | 2005-11-24 |
| AU2005243474A1 (en) | 2005-11-24 |
| ZA200609115B (en) | 2008-06-25 |
| BRPI0510782A (pt) | 2007-11-20 |
| ECSP066989A (es) | 2006-12-29 |
| US20070077274A1 (en) | 2007-04-05 |
| NO20065652L (no) | 2006-12-07 |
| EP1747247A2 (de) | 2007-01-31 |
| DE102004024635A1 (de) | 2005-12-08 |
| WO2005111121A3 (de) | 2006-03-02 |
| EA200602031A1 (ru) | 2007-08-31 |
| CR8427A (es) | 2006-11-23 |
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