JP2006502416A5 - - Google Patents

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JP2006502416A5
JP2006502416A5 JP2004543745A JP2004543745A JP2006502416A5 JP 2006502416 A5 JP2006502416 A5 JP 2006502416A5 JP 2004543745 A JP2004543745 A JP 2004543745A JP 2004543745 A JP2004543745 A JP 2004543745A JP 2006502416 A5 JP2006502416 A5 JP 2006502416A5
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mhc
binding
monomer
binding peptide
putative
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JP2004543745A
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JP4607590B2 (ja
JP2006502416A (ja
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Priority claimed from US10/269,473 external-priority patent/US20040072262A1/en
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Claims (6)

  1. 表面に一つまたはそれ以上のMHC単量体または改変MHC単量体が結合し、該単量体が、溶液から適したMHC結合ペプチドを組み入れる、固体表面を含むシステム。
  2. 請求項1記載のシステムを含むキット。
  3. 以下の段階を含む、MHCクラスI単量体または改変MHCクラスI単量体と、その推定のMHC結合ペプチドとの結合を決定する方法：
    複数のMHC単量体または改変MHC単量体が結合した固体表面を推定のMHC結合ペプチドの存在下および非存在下で再構成条件でインキュベートする段階であって、該単量体が変性され、再構成条件で適したMHC結合ペプチドを含む複合体を形成するように再構成される段階、ならびに
    MHC複合体に結合するが、MHC複合体の解離した成分には結合しないモノクローナル抗体に接触させた後のMHC単量体に対する結合を決定して、該抗体の結合により、単量体と推定のMHC結合ペプチドとが結合していることが示される段階。
  4. 以下の段階を含む、MHCクラスII単量体または改変MHCクラスII単量体と、その推定のMHC結合ペプチドとの結合を決定する方法：
    推定のMHC結合ペプチドの存在下および非存在下で複数のMHC単量体または改変MHC単量体が結合した固体表面を、適したペプチドローディング条件でインキュベートして、適したMHC結合ペプチドを含む複合体を形成する段階、ならびに
    MHC単量体と推定のMHC結合ペプチドとの結合を決定する段階。
  5. 以下の段階を含む、推定のMHC結合ペプチドに関するMHCクラスI単量体または改変MHCクラスI単量体の結合親和性の程度を決定する方法：
    固体表面に結合した少なくとも一つの変性MHC単量体または改変MHC単量体を、推定のMHC結合ペプチド、および対応する再構成MHC単量体を含む第一の複合体における立体配座エピトープに特異的に結合するが、MHC複合体の解離成分には結合しないモノクローナル抗体と共にインキュベートする段階であって、インキュベーションが再構成条件で行われる段階；ならびに
    推定のMHC結合ペプチドを含むMHC複合体に対するモノクローナル抗体の結合を、単量体と既知のMHC結合ペプチドとを含む対応する複合体に対するモノクローナル抗体の結合と比較して、結合の差によって、推定のMHC結合ペプチドに関する再構成単量体の結合親和性の相対度が示される段階。
  6. 以下の段階を含む、推定のMHC結合ペプチドに関するMHCクラスII単量体または改変MHCクラスII単量体の結合親和性の程度を決定する方法：
    固体表面に結合させた少なくとも一つのMHCクラスII単量体または改変MHCクラスII単量体を、推定のMHC結合ペプチド、および単量体を含む第一の複合体におけるエピトープに特異的に結合するが、MHC複合体の解離成分には結合しないモノクローナル抗体と共に、適した結合条件でインキュベートする段階；ならびに
    推定のMHC結合ペプチドを含むMHC複合体に対するモノクローナル抗体の結合を、単量体と既知のMHC結合ペプチドとを含む対応する複合体に対するモノクローナル抗体の結合と比較して、結合の差によって、推定のMHC結合ペプチドに関する単量体の結合親和性の相対度が示される段階。
JP2004543745A 2002-10-11 2003-10-10 Ｍｈｃクラスｉおよびクラスｉｉ結合ペプチドを検出するための方法およびシステム Expired - Lifetime JP4607590B2 (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/269,473 US20040072262A1 (en) 2002-10-11 2002-10-11 Methods and systems for detecting MHC class I binding peptides
PCT/US2003/032370 WO2004034033A2 (en) 2002-10-11 2003-10-10 Methods and systems for detecting mhc class i and class ii binding peptides

Publications (3)

Publication Number Publication Date
JP2006502416A JP2006502416A (ja) 2006-01-19
JP2006502416A5 true JP2006502416A5 (ja) 2006-11-24
JP4607590B2 JP4607590B2 (ja) 2011-01-05

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JP2004543745A Expired - Lifetime JP4607590B2 (ja) 2002-10-11 2003-10-10 Ｍｈｃクラスｉおよびクラスｉｉ結合ペプチドを検出するための方法およびシステム

Country Status (7)

Country Link
US (4) US20040072262A1 (ja)
EP (1) EP1549952B1 (ja)
JP (1) JP4607590B2 (ja)
CN (1) CN1703624B (ja)
AU (1) AU2003284113B2 (ja)
CA (1) CA2501864C (ja)
WO (1) WO2004034033A2 (ja)

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