JP2006502378A5 - - Google Patents

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Publication number
JP2006502378A5
JP2006502378A5 JP2004540055A JP2004540055A JP2006502378A5 JP 2006502378 A5 JP2006502378 A5 JP 2006502378A5 JP 2004540055 A JP2004540055 A JP 2004540055A JP 2004540055 A JP2004540055 A JP 2004540055A JP 2006502378 A5 JP2006502378 A5 JP 2006502378A5
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JP
Japan
Prior art keywords
substrate
bont
cell
acceptor
clostridial toxin
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Application number
JP2004540055A
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English (en)
Japanese (ja)
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JP4554369B2 (ja
JP2006502378A (ja
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Publication date
Priority claimed from US10/261,161 external-priority patent/US7183066B2/en
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Publication of JP2006502378A publication Critical patent/JP2006502378A/ja
Publication of JP2006502378A5 publication Critical patent/JP2006502378A5/ja
Application granted granted Critical
Publication of JP4554369B2 publication Critical patent/JP4554369B2/ja
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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JP2004540055A 2002-09-27 2003-09-04 クロストリジウム毒素に関する細胞ベースの蛍光共鳴エネルギー転移(fret)アッセイ Expired - Fee Related JP4554369B2 (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/261,161 US7183066B2 (en) 2002-09-27 2002-09-27 Cell-based fluorescence resonance energy transfer (FRET) assays for clostridial toxins
PCT/US2003/028092 WO2004029576A2 (en) 2002-09-27 2003-09-04 Cell-based fluorescence resonance energy transfer (fret) assays for clostridial toxins

Publications (3)

Publication Number Publication Date
JP2006502378A JP2006502378A (ja) 2006-01-19
JP2006502378A5 true JP2006502378A5 (https=) 2006-10-12
JP4554369B2 JP4554369B2 (ja) 2010-09-29

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Family Applications (1)

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JP2004540055A Expired - Fee Related JP4554369B2 (ja) 2002-09-27 2003-09-04 クロストリジウム毒素に関する細胞ベースの蛍光共鳴エネルギー転移(fret)アッセイ

Country Status (17)

Country Link
US (3) US7183066B2 (https=)
EP (1) EP1543329B1 (https=)
JP (1) JP4554369B2 (https=)
KR (1) KR20050071506A (https=)
CN (1) CN1296712C (https=)
AU (2) AU2003282790B2 (https=)
BR (1) BR0314795A (https=)
CA (1) CA2500040C (https=)
CO (1) CO5721032A2 (https=)
DK (1) DK1543329T3 (https=)
IL (1) IL167410A (https=)
MX (1) MXPA05003128A (https=)
NO (1) NO20051049L (https=)
PL (1) PL376103A1 (https=)
RU (1) RU2005109385A (https=)
WO (1) WO2004029576A2 (https=)
ZA (1) ZA200501500B (https=)

Families Citing this family (72)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080038274A1 (en) * 1999-09-23 2008-02-14 Foster Keith A Inhibition of secretion from non-neuronal cells
US7354735B2 (en) * 2001-05-24 2008-04-08 Hong Kong University Of Science And Technology Nucleic acid encoding fluorescent protein constructs and methods for detecting apoptosis
US20080064054A1 (en) * 2001-08-28 2008-03-13 Ester Fernandez-Salas Fluorescence resonance energy transfer (fret) assays for clostridial toxin activity
US7332567B2 (en) * 2001-08-28 2008-02-19 Allergan, Inc. Fret protease assays for clostridial toxins
US8022172B2 (en) * 2001-08-28 2011-09-20 Allergan, Inc. Luminescence resonance energy transfer (LRET) assays for clostridial toxin activity
US7374896B2 (en) 2001-08-28 2008-05-20 Allergan, Inc. GFP-SNAP25 fluorescence release assay for botulinum neurotoxin protease activity
US20060063221A1 (en) * 2004-09-21 2006-03-23 Williams Dudley J Lanthanide-based substrates and methods for determining clostridial toxin activity
US7208285B2 (en) * 2001-08-28 2007-04-24 Allergan, Inc. Fret protease assays for botulinum serotype A/E toxins
US7183066B2 (en) 2002-09-27 2007-02-27 Allergan, Inc. Cell-based fluorescence resonance energy transfer (FRET) assays for clostridial toxins
WO2004031355A2 (en) * 2002-10-01 2004-04-15 University Of Maryland Methods for identifying inhibitors of botulinum neurotoxins
WO2005076785A2 (en) * 2003-12-19 2005-08-25 Wisconsin Alumni Research Foundation Method and compositions for detecting botulinum neurotoxin
ES2374822T3 (es) * 2004-02-24 2012-02-22 Allergan, Inc. Ensayo de selección de toxinas botul�?nicas.
US8497081B2 (en) * 2004-02-24 2013-07-30 Allergan, Inc. Botulinum toxin screening assays
DE602005011458D1 (de) * 2004-09-01 2009-01-15 Allergan Inc Abbaubare clostridientoxine
US8617838B2 (en) * 2004-09-20 2013-12-31 University Of Massachusetts Fluorescent proteins and related methods and compounds
US7399607B2 (en) * 2004-09-22 2008-07-15 Allergan, Inc. Fluorescence polarization assays for determining clostridial toxin activity
US8031338B2 (en) * 2004-12-02 2011-10-04 Vanderbilt University Measuring Forster resonance energy transfer with polarized and depolarized light
CA2605160A1 (en) 2005-04-05 2006-10-05 Allergan, Inc. Clostridial toxin activity assays
EP2293064B1 (en) * 2005-04-05 2012-04-04 Allergan, Inc. Lipophilic dye-based FRET assays for clostridial toxin activity
DE102005020384A1 (de) * 2005-05-02 2006-11-09 Therainvention Gmbh Spektroskopisches Verfahren zum Nachweis von Analyten
ATE518882T1 (de) * 2005-09-19 2011-08-15 Allergan Inc Mit clostridientoxin aktivierbare clostridientoxine
CA2602972C (en) * 2005-10-12 2013-09-24 Allergan, Inc. Assays of molecular or subcellular interactivity using depolarization after resonance energy transfer (daret)
FR2903495A1 (fr) * 2006-07-06 2008-01-11 Pharmaleads Soc Par Actions Si PROCEDE DE DETECTION D'INTERACTIONS A L'AIDE DU RAPPORTEUR DE FLUORESCENCE Pya
JP2008220302A (ja) * 2007-03-14 2008-09-25 Hiroshima Univ タンパク質蓄積による神経変性疾患治療薬物のスクリーニング系
US7733470B2 (en) * 2007-06-26 2010-06-08 Photon Technology International, Inc. Method and apparatus for real-time measurement and calculation of a fluorescent lifetime
WO2009035476A1 (en) 2007-09-14 2009-03-19 Biosentinel, Llc Resonance energy transfer assay with cleavage sequence and spacer
PT2271670E (pt) 2008-03-14 2014-11-28 Allergan Inc Ensaio imunobaseado da atividade do serotipo a da toxina botulínica
KR101604515B1 (ko) * 2008-03-14 2016-03-17 알러간, 인코포레이티드 면역-기반 보툴리눔 독소 세로타입 a 활성 검정
CN105833254A (zh) 2008-12-10 2016-08-10 阿勒根公司 梭菌毒素药物组合物
WO2010085473A1 (en) 2009-01-20 2010-07-29 Trustees Of Tufts College Methods for the delivery of toxins or enzymatically active portions thereof
BRPI1008940A2 (pt) 2009-03-13 2020-10-27 Allergan, Inc. linhagem de célula clonal estabelecida suscetível à intoxicação por bont/a
KR101923847B1 (ko) * 2009-03-13 2018-11-29 알러간, 인코포레이티드 면역 기반 재표적화된 엔도펩티다제 활성 검정
US9247611B2 (en) * 2009-06-01 2016-01-26 Apple Inc. Light source with light sensor
US8129139B2 (en) 2009-07-13 2012-03-06 Allergan, Inc. Process for obtaining botulinum neurotoxin
US8926561B2 (en) 2009-07-30 2015-01-06 Tandem Diabetes Care, Inc. Infusion pump system with disposable cartridge having pressure venting and pressure feedback
US20120183440A1 (en) * 2009-09-29 2012-07-19 Mitsui Engineering & Shipbuilding Co.,Ltd. Fret measurement method and device
EP2483666A1 (en) 2009-10-02 2012-08-08 Allergan, Inc. In vitro method of determining changes in a protein environment
FR2958293B1 (fr) 2010-04-01 2014-09-26 Centre Nat Rech Scient Outils pour l'identification de ligands de lingo-1,lingo-2, lingo-3 et lingo-4, et utilisations
CN102243176B (zh) * 2010-05-14 2013-11-20 中国人民解放军军事医学科学院毒物药物研究所 一种体外分子水平荧光法排除筛选药物假阳性结果的方法
US8940482B1 (en) * 2010-06-11 2015-01-27 Synaptic Research, Llc N-end rule protease activity indication methods and uses thereof
CN103228669B (zh) * 2010-09-27 2016-02-24 国立大学法人京都大学 基于荧光共振能量转移的原理的单分子型fret生物传感器接头
US8569254B2 (en) 2010-12-10 2013-10-29 National Yang Ming University Methods for modulating the expression and aggregation of CAG-expanded gene product in cells and methods for identifying agents useful for doing the same
US11325954B2 (en) 2011-06-01 2022-05-10 Biomadison, Inc. Compositions and methods for stability testing of botulinum toxin
US9303285B2 (en) 2012-01-04 2016-04-05 Biomadison, Inc. Methods and compounds for increasing sensitivity of botulinum assays
US10908146B2 (en) 2011-06-01 2021-02-02 Biomadison, Inc. Compositions and methods for improving sensitivity in cell based assays
KR101853103B1 (ko) 2011-06-01 2018-04-27 바이오메디슨, 인코퍼레이티드 비-fret 보툴리눔 검정법
US12422428B2 (en) 2011-06-01 2025-09-23 Biomadison, Inc. Compositions and methods for cell-based assays
EP2798077A2 (en) 2011-12-31 2014-11-05 Allergan, Inc. Highly Sensitive Cell-Based Assay to Detect the Presence of Active Botulinum Neurotoxin Serotype-A
WO2013103983A1 (en) * 2012-01-06 2013-07-11 The Research Foundation Of State University Of New York High throughput screening of live cells
GB201219024D0 (en) * 2012-10-23 2012-12-05 Syntaxin Ltd Assay
LT3030905T (lt) 2013-08-09 2018-01-25 Biomadison, Inc. Padidinto jautrumo botulino toksino tyrimas
WO2015061738A1 (en) * 2013-10-25 2015-04-30 Biomadison, Inc. Enzyme assay with duplicate fluorophores
KR20170026624A (ko) 2014-07-07 2017-03-08 알러간, 인코포레이티드 조직 샘플에서 절단된 snap25를 검출하는 방법
AU2015301737B2 (en) * 2014-08-12 2021-01-28 Biomadison, Inc. Botulinum neurotoxins with modified light chain specifity and methods for producing same
CN104359883A (zh) * 2014-11-13 2015-02-18 贵阳医学院 人源沉默信息调节因子6的活性荧光检测方法
EP3590500B1 (en) 2014-12-23 2025-06-25 Merz Pharma GmbH & Co. KGaA Botulinum toxin prefilled container
US10676723B2 (en) 2015-05-11 2020-06-09 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
CN105784656B (zh) * 2016-03-16 2019-01-01 大连理工大学 一种检测活细胞内RhoGDIα蛋白活性的生物探针
TWI737742B (zh) 2016-06-22 2021-09-01 德商梅茲製藥有限兩合公司 肉毒桿菌毒素的預填充式注射器系統、具有其之套組以及其之使用
KR102480965B1 (ko) 2016-09-13 2022-12-26 알레간 인코포레이티드 안정화된 비단백질 클로스트리듐 독소 조성물
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
CN108305912B (zh) * 2017-01-11 2024-03-26 中国科学院上海微系统与信息技术研究所 具有波长选择性的石墨烯仿生光探测器及其制备方法
WO2018224561A1 (en) * 2017-06-07 2018-12-13 Original G B.V. Method for detecting food spoilage microbes
US12158470B2 (en) * 2018-03-09 2024-12-03 Sartorius Bioanalytical Instruments, Inc. Methods and compositions for live cell analysis of intracellular ATP
CN108333342A (zh) * 2018-05-14 2018-07-27 广东药科大学 一种快速检测牛奶中四环素类药物残留的方法
CA3111674A1 (en) * 2018-09-28 2020-04-02 Ipsen Biopharm Limited Cell-based clostridal neurotoxin assays
CN109211863B (zh) * 2018-10-26 2021-04-20 大连民族大学 利用Eu2+f-f跃迁光谱检测爆炸物TNP的方法
TW202113072A (zh) * 2019-06-07 2021-04-01 美商辛那皮克研究有限責任公司 基因工程化細胞
US12416634B2 (en) * 2020-06-01 2025-09-16 Galderma Holding SA SNAP-25 reporter constructs and methods of using the same
CN112557353B (zh) * 2020-12-17 2022-02-18 天津工业大学 一种基于延迟发光光谱的细胞活力检测方法和装置
EP4621057A1 (en) * 2022-11-18 2025-09-24 Biolinks Inc. Method for detection or activity measurement of cell-based botulinum toxin by using recombinant fluorescent protein
KR20250158928A (ko) * 2024-04-30 2025-11-07 주식회사 바이오링크스 앵커 단백질을 이용한 미광성 세포기반의 보툴리눔 독소의 검출 및 활성 평가방법

Family Cites Families (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5804604A (en) * 1989-12-21 1998-09-08 Biogen, Inc. Tat-derived transport polypeptides and fusion proteins
JPH05505102A (ja) * 1989-12-21 1993-08-05 ホワイトヘツド・インスチチユート・フオー・バイオメデイカル・リサーチ 真核細胞の中に分子を配達する方法
US5962637A (en) * 1994-06-03 1999-10-05 Microbiological Research Authority Toxin assay
GB9411138D0 (en) * 1994-06-03 1994-07-27 Microbiological Res Authority Toxin assay
GB9508204D0 (en) * 1995-04-21 1995-06-07 Speywood Lab Ltd A novel agent able to modify peripheral afferent function
US6069010A (en) 1995-09-11 2000-05-30 Axys Pharmaceuticals, Inc. High throughput gene inactivation with large scale gene targeting
US6803188B1 (en) * 1996-01-31 2004-10-12 The Regents Of The University Of California Tandem fluorescent protein constructs
US5965699A (en) * 1996-11-06 1999-10-12 The United States Of America As Represented By The Secretary Of The Army Assay for the proteolytic activity of serotype a from clostridium botulinum
WO1999029721A1 (en) 1997-12-10 1999-06-17 Washington University Anti-pathogen system and methods of use thereof
EP1073763A4 (en) 1998-04-28 2002-08-07 Univ Washington METHODS FOR TRANSDUCING FUSION MOLECULES
US7090976B2 (en) * 1999-11-10 2006-08-15 Rigel Pharmaceuticals, Inc. Methods and compositions comprising Renilla GFP
DE69903337T2 (de) 1998-10-30 2003-08-21 Cellomics, Inc. Ein screeningsystem auf zellbasis
DE19856897A1 (de) * 1998-12-10 2000-06-15 Biotecon Ges Fuer Biotechnologische Entwicklung & Consulting Mbh Therapeutikum zur Unterdrückung von Schnarchgeräuschen
JP2002531113A (ja) 1998-12-10 2002-09-24 ワシントン大学 蛋白質の形質導入システムおよびその使用法
US20020076741A1 (en) 2000-02-11 2002-06-20 Tencza Sarah Burroughs Peptide biosensors for anthrax protease
US6325899B1 (en) * 2000-03-10 2001-12-04 Action Caps, Llc Disposable and recyclable intermediates for use in electrostatic coating processes
FR2809733B1 (fr) * 2000-06-02 2004-04-30 Inst Nat Sante Rech Med Substrat peptidique reconnu par la toxine botulique de type bont/b et son utilisation pour doser et/ou detecter ladite toxine ou des inhibiteurs correspondants
DE60131468D1 (de) 2000-09-25 2007-12-27 U S Medical Res Inst Of Infect Hoch-durchsatz assays für proteolytische aktivitäten von clostridium neurotoxinen
AU2002217839A1 (en) * 2000-11-22 2002-06-03 The Regents Of The University Of California A method to measure the activation state of signaling pathways in cells
CA2390686A1 (en) * 2001-06-14 2002-12-14 Pierre Chambon Cofactor-based screening method for nuclear receptor modulators and related modulators
US20080064054A1 (en) * 2001-08-28 2008-03-13 Ester Fernandez-Salas Fluorescence resonance energy transfer (fret) assays for clostridial toxin activity
US8022172B2 (en) * 2001-08-28 2011-09-20 Allergan, Inc. Luminescence resonance energy transfer (LRET) assays for clostridial toxin activity
US7208285B2 (en) * 2001-08-28 2007-04-24 Allergan, Inc. Fret protease assays for botulinum serotype A/E toxins
US7332567B2 (en) * 2001-08-28 2008-02-19 Allergan, Inc. Fret protease assays for clostridial toxins
US7374896B2 (en) * 2001-08-28 2008-05-20 Allergan, Inc. GFP-SNAP25 fluorescence release assay for botulinum neurotoxin protease activity
US7183066B2 (en) * 2002-09-27 2007-02-27 Allergan, Inc. Cell-based fluorescence resonance energy transfer (FRET) assays for clostridial toxins
ES2374822T3 (es) * 2004-02-24 2012-02-22 Allergan, Inc. Ensayo de selección de toxinas botul�?nicas.
US7399607B2 (en) * 2004-09-22 2008-07-15 Allergan, Inc. Fluorescence polarization assays for determining clostridial toxin activity
EP2293064B1 (en) * 2005-04-05 2012-04-04 Allergan, Inc. Lipophilic dye-based FRET assays for clostridial toxin activity
CA2602972C (en) * 2005-10-12 2013-09-24 Allergan, Inc. Assays of molecular or subcellular interactivity using depolarization after resonance energy transfer (daret)

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