RU2005109385A - Клеточные анализы резонаннсного переноса энергии флуоресценции (fret) клостридиальных токсинов - Google Patents
Клеточные анализы резонаннсного переноса энергии флуоресценции (fret) клостридиальных токсинов Download PDFInfo
- Publication number
- RU2005109385A RU2005109385A RU2005109385/15A RU2005109385A RU2005109385A RU 2005109385 A RU2005109385 A RU 2005109385A RU 2005109385/15 A RU2005109385/15 A RU 2005109385/15A RU 2005109385 A RU2005109385 A RU 2005109385A RU 2005109385 A RU2005109385 A RU 2005109385A
- Authority
- RU
- Russia
- Prior art keywords
- substrate
- cell
- bont
- composition according
- specified
- Prior art date
Links
- 231100001102 clostridial toxin Toxicity 0.000 title claims 25
- 238000004458 analytical method Methods 0.000 title 1
- 238000002866 fluorescence resonance energy transfer Methods 0.000 title 1
- 239000000758 substrate Substances 0.000 claims 80
- 210000004027 cell Anatomy 0.000 claims 49
- 108030001720 Bontoxilysin Proteins 0.000 claims 46
- 238000000034 method Methods 0.000 claims 22
- 239000000816 peptidomimetic Substances 0.000 claims 13
- 229940053031 botulinum toxin Drugs 0.000 claims 10
- 229940124447 delivery agent Drugs 0.000 claims 9
- 210000002569 neuron Anatomy 0.000 claims 9
- 238000003776 cleavage reaction Methods 0.000 claims 8
- 230000007017 scission Effects 0.000 claims 8
- 230000000694 effects Effects 0.000 claims 5
- 239000012634 fragment Substances 0.000 claims 5
- 230000005855 radiation Effects 0.000 claims 5
- 230000001105 regulatory effect Effects 0.000 claims 5
- 238000000862 absorption spectrum Methods 0.000 claims 4
- 238000000295 emission spectrum Methods 0.000 claims 4
- 230000001939 inductive effect Effects 0.000 claims 4
- 108020004707 nucleic acids Proteins 0.000 claims 4
- 102000039446 nucleic acids Human genes 0.000 claims 4
- 150000007523 nucleic acids Chemical class 0.000 claims 4
- 101000574352 Mus musculus Protein phosphatase 1 regulatory subunit 17 Proteins 0.000 claims 3
- 125000003275 alpha amino acid group Chemical group 0.000 claims 3
- 108090000765 processed proteins & peptides Proteins 0.000 claims 3
- 230000007888 toxin activity Effects 0.000 claims 3
- 108700031308 Antennapedia Homeodomain Proteins 0.000 claims 2
- VHLZDSUANXBJHW-QWRGUYRKSA-N Gln-Phe Chemical compound NC(=O)CC[C@H](N)C(=O)N[C@H](C(O)=O)CC1=CC=CC=C1 VHLZDSUANXBJHW-QWRGUYRKSA-N 0.000 claims 2
- 108010043121 Green Fluorescent Proteins Proteins 0.000 claims 2
- 102000004144 Green Fluorescent Proteins Human genes 0.000 claims 2
- 206010029260 Neuroblastoma Diseases 0.000 claims 2
- 241000700584 Simplexvirus Species 0.000 claims 2
- 101710192266 Tegument protein VP22 Proteins 0.000 claims 2
- 210000003169 central nervous system Anatomy 0.000 claims 2
- 230000005281 excited state Effects 0.000 claims 2
- 239000005090 green fluorescent protein Substances 0.000 claims 2
- 210000005260 human cell Anatomy 0.000 claims 2
- 102000004169 proteins and genes Human genes 0.000 claims 2
- 108090000623 proteins and genes Proteins 0.000 claims 2
- RAVVEEJGALCVIN-AGVBWZICSA-N (2s)-2-[[(2s)-2-[[(2s)-2-[[(2s)-5-amino-2-[[(2s)-2-[[(2s)-2-[[(2s)-6-amino-2-[[(2s)-6-amino-2-[[(2s)-2-[[2-[[(2s)-2-amino-3-(4-hydroxyphenyl)propanoyl]amino]acetyl]amino]-5-(diaminomethylideneamino)pentanoyl]amino]hexanoyl]amino]hexanoyl]amino]-5-(diamino Chemical compound NC(N)=NCCC[C@@H](C(O)=O)NC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCCN=C(N)N)NC(=O)CNC(=O)[C@@H](N)CC1=CC=C(O)C=C1 RAVVEEJGALCVIN-AGVBWZICSA-N 0.000 claims 1
- WCKQPPQRFNHPRJ-UHFFFAOYSA-N 4-[[4-(dimethylamino)phenyl]diazenyl]benzoic acid Chemical compound C1=CC(N(C)C)=CC=C1N=NC1=CC=C(C(O)=O)C=C1 WCKQPPQRFNHPRJ-UHFFFAOYSA-N 0.000 claims 1
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 1
- SJQRQOKXQKVJGJ-UHFFFAOYSA-N 5-(2-aminoethylamino)naphthalene-1-sulfonic acid Chemical compound C1=CC=C2C(NCCN)=CC=CC2=C1S(O)(=O)=O SJQRQOKXQKVJGJ-UHFFFAOYSA-N 0.000 claims 1
- 239000012099 Alexa Fluor family Substances 0.000 claims 1
- WVRUNFYJIHNFKD-WDSKDSINSA-N Arg-Ala Chemical compound OC(=O)[C@H](C)NC(=O)[C@@H](N)CCCN=C(N)N WVRUNFYJIHNFKD-WDSKDSINSA-N 0.000 claims 1
- QYLJIYOGHRGUIH-CIUDSAMLSA-N Arg-Ile Chemical compound CC[C@H](C)[C@@H](C(O)=O)NC(=O)[C@@H](N)CCCNC(N)=N QYLJIYOGHRGUIH-CIUDSAMLSA-N 0.000 claims 1
- 101000609447 Beet necrotic yellow vein virus (isolate Japan/S) Protein P25 Proteins 0.000 claims 1
- UPEZCKBFRMILAV-JNEQICEOSA-N Ecdysone Natural products O=C1[C@H]2[C@@](C)([C@@H]3C([C@@]4(O)[C@@](C)([C@H]([C@H]([C@@H](O)CCC(O)(C)C)C)CC4)CC3)=C1)C[C@H](O)[C@H](O)C2 UPEZCKBFRMILAV-JNEQICEOSA-N 0.000 claims 1
- 208000003098 Ganglion Cysts Diseases 0.000 claims 1
- OPINTGHFESTVAX-BQBZGAKWSA-N Gln-Arg Chemical compound NC(=O)CC[C@H](N)C(=O)N[C@H](C(O)=O)CCCN=C(N)N OPINTGHFESTVAX-BQBZGAKWSA-N 0.000 claims 1
- CLSDNFWKGFJIBZ-YUMQZZPRSA-N Gln-Lys Chemical compound NCCCC[C@@H](C(O)=O)NC(=O)[C@@H](N)CCC(N)=O CLSDNFWKGFJIBZ-YUMQZZPRSA-N 0.000 claims 1
- 108010070875 Human Immunodeficiency Virus tat Gene Products Proteins 0.000 claims 1
- 108700000788 Human immunodeficiency virus 1 tat peptide (47-57) Proteins 0.000 claims 1
- DEFJQIDDEAULHB-IMJSIDKUSA-N L-alanyl-L-alanine Chemical compound C[C@H](N)C(=O)N[C@@H](C)C(O)=O DEFJQIDDEAULHB-IMJSIDKUSA-N 0.000 claims 1
- QOOWRKBDDXQRHC-BQBZGAKWSA-N L-lysyl-L-alanine Chemical compound OC(=O)[C@H](C)NC(=O)[C@@H](N)CCCCN QOOWRKBDDXQRHC-BQBZGAKWSA-N 0.000 claims 1
- ATIPDCIQTUXABX-UWVGGRQHSA-N Lys-Leu Chemical compound CC(C)C[C@@H](C(O)=O)NC(=O)[C@@H](N)CCCCN ATIPDCIQTUXABX-UWVGGRQHSA-N 0.000 claims 1
- 108010047702 MPG peptide Proteins 0.000 claims 1
- 102000018697 Membrane Proteins Human genes 0.000 claims 1
- 108010052285 Membrane Proteins Proteins 0.000 claims 1
- 101710149951 Protein Tat Proteins 0.000 claims 1
- 108010010469 Qa-SNARE Proteins Proteins 0.000 claims 1
- 208000005400 Synovial Cyst Diseases 0.000 claims 1
- 102000050389 Syntaxin Human genes 0.000 claims 1
- 108030001722 Tentoxilysin Proteins 0.000 claims 1
- 239000004098 Tetracycline Substances 0.000 claims 1
- 108010057266 Type A Botulinum Toxins Proteins 0.000 claims 1
- 239000012190 activator Substances 0.000 claims 1
- 108010056243 alanylalanine Proteins 0.000 claims 1
- UPEZCKBFRMILAV-UHFFFAOYSA-N alpha-Ecdysone Natural products C1C(O)C(O)CC2(C)C(CCC3(C(C(C(O)CCC(C)(C)O)C)CCC33O)C)C3=CC(=O)C21 UPEZCKBFRMILAV-UHFFFAOYSA-N 0.000 claims 1
- 238000003556 assay Methods 0.000 claims 1
- 239000013592 cell lysate Substances 0.000 claims 1
- 210000003710 cerebral cortex Anatomy 0.000 claims 1
- 230000002490 cerebral effect Effects 0.000 claims 1
- BHONFOAYRQZPKZ-LCLOTLQISA-N chembl269478 Chemical compound C([C@H](NC(=O)[C@H](CC=1C2=CC=CC=C2NC=1)NC(=O)[C@H]([C@@H](C)CC)NC(=O)[C@H](CCCCN)NC(=O)[C@@H](NC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](N)CCCNC(N)=N)[C@@H](C)CC)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(O)=O)C1=CC=CC=C1 BHONFOAYRQZPKZ-LCLOTLQISA-N 0.000 claims 1
- 108091006116 chimeric peptides Proteins 0.000 claims 1
- UPEZCKBFRMILAV-JMZLNJERSA-N ecdysone Chemical compound C1[C@@H](O)[C@@H](O)C[C@]2(C)[C@@H](CC[C@@]3([C@@H]([C@@H]([C@H](O)CCC(C)(C)O)C)CC[C@]33O)C)C3=CC(=O)[C@@H]21 UPEZCKBFRMILAV-JMZLNJERSA-N 0.000 claims 1
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 claims 1
- 108020001507 fusion proteins Proteins 0.000 claims 1
- 102000037865 fusion proteins Human genes 0.000 claims 1
- 210000004295 hippocampal neuron Anatomy 0.000 claims 1
- 210000002161 motor neuron Anatomy 0.000 claims 1
- 210000000496 pancreas Anatomy 0.000 claims 1
- 210000002856 peripheral neuron Anatomy 0.000 claims 1
- 210000000278 spinal cord Anatomy 0.000 claims 1
- 229960002180 tetracycline Drugs 0.000 claims 1
- 229930101283 tetracycline Natural products 0.000 claims 1
- 235000019364 tetracycline Nutrition 0.000 claims 1
- 150000003522 tetracyclines Chemical class 0.000 claims 1
- 231100000765 toxin Toxicity 0.000 claims 1
- 239000003053 toxin Substances 0.000 claims 1
- 108700012359 toxins Proteins 0.000 claims 1
- 238000013518 transcription Methods 0.000 claims 1
- 230000035897 transcription Effects 0.000 claims 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/554—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being a biological cell or cell fragment, e.g. bacteria, yeast cells
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/33—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Clostridium (G)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4702—Regulators; Modulating activity
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4725—Proteoglycans, e.g. aggreccan
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/16011—Herpesviridae
- C12N2710/16611—Simplexvirus, e.g. human herpesvirus 1, 2
- C12N2710/16622—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/16011—Human Immunodeficiency Virus, HIV
- C12N2740/16111—Human Immunodeficiency Virus, HIV concerning HIV env
- C12N2740/16122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Zoology (AREA)
- Immunology (AREA)
- Gastroenterology & Hepatology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Urology & Nephrology (AREA)
- Toxicology (AREA)
- Hematology (AREA)
- Virology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Cell Biology (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- Pharmacology & Pharmacy (AREA)
- Communicable Diseases (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Mycology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oncology (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US10/261161 | 2002-09-27 | ||
| US10/261,161 US7183066B2 (en) | 2002-09-27 | 2002-09-27 | Cell-based fluorescence resonance energy transfer (FRET) assays for clostridial toxins |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| RU2005109385A true RU2005109385A (ru) | 2006-01-27 |
Family
ID=32041812
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2005109385/15A RU2005109385A (ru) | 2002-09-27 | 2003-09-04 | Клеточные анализы резонаннсного переноса энергии флуоресценции (fret) клостридиальных токсинов |
Country Status (17)
| Country | Link |
|---|---|
| US (3) | US7183066B2 (https=) |
| EP (1) | EP1543329B1 (https=) |
| JP (1) | JP4554369B2 (https=) |
| KR (1) | KR20050071506A (https=) |
| CN (1) | CN1296712C (https=) |
| AU (2) | AU2003282790B2 (https=) |
| BR (1) | BR0314795A (https=) |
| CA (1) | CA2500040C (https=) |
| CO (1) | CO5721032A2 (https=) |
| DK (1) | DK1543329T3 (https=) |
| IL (1) | IL167410A (https=) |
| MX (1) | MXPA05003128A (https=) |
| NO (1) | NO20051049L (https=) |
| PL (1) | PL376103A1 (https=) |
| RU (1) | RU2005109385A (https=) |
| WO (1) | WO2004029576A2 (https=) |
| ZA (1) | ZA200501500B (https=) |
Families Citing this family (72)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080038274A1 (en) * | 1999-09-23 | 2008-02-14 | Foster Keith A | Inhibition of secretion from non-neuronal cells |
| US7354735B2 (en) * | 2001-05-24 | 2008-04-08 | Hong Kong University Of Science And Technology | Nucleic acid encoding fluorescent protein constructs and methods for detecting apoptosis |
| US20080064054A1 (en) * | 2001-08-28 | 2008-03-13 | Ester Fernandez-Salas | Fluorescence resonance energy transfer (fret) assays for clostridial toxin activity |
| US7332567B2 (en) * | 2001-08-28 | 2008-02-19 | Allergan, Inc. | Fret protease assays for clostridial toxins |
| US8022172B2 (en) * | 2001-08-28 | 2011-09-20 | Allergan, Inc. | Luminescence resonance energy transfer (LRET) assays for clostridial toxin activity |
| US7374896B2 (en) | 2001-08-28 | 2008-05-20 | Allergan, Inc. | GFP-SNAP25 fluorescence release assay for botulinum neurotoxin protease activity |
| US20060063221A1 (en) * | 2004-09-21 | 2006-03-23 | Williams Dudley J | Lanthanide-based substrates and methods for determining clostridial toxin activity |
| US7208285B2 (en) * | 2001-08-28 | 2007-04-24 | Allergan, Inc. | Fret protease assays for botulinum serotype A/E toxins |
| US7183066B2 (en) | 2002-09-27 | 2007-02-27 | Allergan, Inc. | Cell-based fluorescence resonance energy transfer (FRET) assays for clostridial toxins |
| WO2004031355A2 (en) * | 2002-10-01 | 2004-04-15 | University Of Maryland | Methods for identifying inhibitors of botulinum neurotoxins |
| WO2005076785A2 (en) * | 2003-12-19 | 2005-08-25 | Wisconsin Alumni Research Foundation | Method and compositions for detecting botulinum neurotoxin |
| ES2374822T3 (es) * | 2004-02-24 | 2012-02-22 | Allergan, Inc. | Ensayo de selección de toxinas botul�?nicas. |
| US8497081B2 (en) * | 2004-02-24 | 2013-07-30 | Allergan, Inc. | Botulinum toxin screening assays |
| DE602005011458D1 (de) * | 2004-09-01 | 2009-01-15 | Allergan Inc | Abbaubare clostridientoxine |
| US8617838B2 (en) * | 2004-09-20 | 2013-12-31 | University Of Massachusetts | Fluorescent proteins and related methods and compounds |
| US7399607B2 (en) * | 2004-09-22 | 2008-07-15 | Allergan, Inc. | Fluorescence polarization assays for determining clostridial toxin activity |
| US8031338B2 (en) * | 2004-12-02 | 2011-10-04 | Vanderbilt University | Measuring Forster resonance energy transfer with polarized and depolarized light |
| CA2605160A1 (en) | 2005-04-05 | 2006-10-05 | Allergan, Inc. | Clostridial toxin activity assays |
| EP2293064B1 (en) * | 2005-04-05 | 2012-04-04 | Allergan, Inc. | Lipophilic dye-based FRET assays for clostridial toxin activity |
| DE102005020384A1 (de) * | 2005-05-02 | 2006-11-09 | Therainvention Gmbh | Spektroskopisches Verfahren zum Nachweis von Analyten |
| ATE518882T1 (de) * | 2005-09-19 | 2011-08-15 | Allergan Inc | Mit clostridientoxin aktivierbare clostridientoxine |
| CA2602972C (en) * | 2005-10-12 | 2013-09-24 | Allergan, Inc. | Assays of molecular or subcellular interactivity using depolarization after resonance energy transfer (daret) |
| FR2903495A1 (fr) * | 2006-07-06 | 2008-01-11 | Pharmaleads Soc Par Actions Si | PROCEDE DE DETECTION D'INTERACTIONS A L'AIDE DU RAPPORTEUR DE FLUORESCENCE Pya |
| JP2008220302A (ja) * | 2007-03-14 | 2008-09-25 | Hiroshima Univ | タンパク質蓄積による神経変性疾患治療薬物のスクリーニング系 |
| US7733470B2 (en) * | 2007-06-26 | 2010-06-08 | Photon Technology International, Inc. | Method and apparatus for real-time measurement and calculation of a fluorescent lifetime |
| WO2009035476A1 (en) | 2007-09-14 | 2009-03-19 | Biosentinel, Llc | Resonance energy transfer assay with cleavage sequence and spacer |
| PT2271670E (pt) | 2008-03-14 | 2014-11-28 | Allergan Inc | Ensaio imunobaseado da atividade do serotipo a da toxina botulínica |
| KR101604515B1 (ko) * | 2008-03-14 | 2016-03-17 | 알러간, 인코포레이티드 | 면역-기반 보툴리눔 독소 세로타입 ａ 활성 검정 |
| CN105833254A (zh) | 2008-12-10 | 2016-08-10 | 阿勒根公司 | 梭菌毒素药物组合物 |
| WO2010085473A1 (en) | 2009-01-20 | 2010-07-29 | Trustees Of Tufts College | Methods for the delivery of toxins or enzymatically active portions thereof |
| BRPI1008940A2 (pt) | 2009-03-13 | 2020-10-27 | Allergan, Inc. | linhagem de célula clonal estabelecida suscetível à intoxicação por bont/a |
| KR101923847B1 (ko) * | 2009-03-13 | 2018-11-29 | 알러간, 인코포레이티드 | 면역 기반 재표적화된 엔도펩티다제 활성 검정 |
| US9247611B2 (en) * | 2009-06-01 | 2016-01-26 | Apple Inc. | Light source with light sensor |
| US8129139B2 (en) | 2009-07-13 | 2012-03-06 | Allergan, Inc. | Process for obtaining botulinum neurotoxin |
| US8926561B2 (en) | 2009-07-30 | 2015-01-06 | Tandem Diabetes Care, Inc. | Infusion pump system with disposable cartridge having pressure venting and pressure feedback |
| US20120183440A1 (en) * | 2009-09-29 | 2012-07-19 | Mitsui Engineering & Shipbuilding Co.,Ltd. | Fret measurement method and device |
| EP2483666A1 (en) | 2009-10-02 | 2012-08-08 | Allergan, Inc. | In vitro method of determining changes in a protein environment |
| FR2958293B1 (fr) | 2010-04-01 | 2014-09-26 | Centre Nat Rech Scient | Outils pour l'identification de ligands de lingo-1,lingo-2, lingo-3 et lingo-4, et utilisations |
| CN102243176B (zh) * | 2010-05-14 | 2013-11-20 | 中国人民解放军军事医学科学院毒物药物研究所 | 一种体外分子水平荧光法排除筛选药物假阳性结果的方法 |
| US8940482B1 (en) * | 2010-06-11 | 2015-01-27 | Synaptic Research, Llc | N-end rule protease activity indication methods and uses thereof |
| CN103228669B (zh) * | 2010-09-27 | 2016-02-24 | 国立大学法人京都大学 | 基于荧光共振能量转移的原理的单分子型fret生物传感器接头 |
| US8569254B2 (en) | 2010-12-10 | 2013-10-29 | National Yang Ming University | Methods for modulating the expression and aggregation of CAG-expanded gene product in cells and methods for identifying agents useful for doing the same |
| US11325954B2 (en) | 2011-06-01 | 2022-05-10 | Biomadison, Inc. | Compositions and methods for stability testing of botulinum toxin |
| US9303285B2 (en) | 2012-01-04 | 2016-04-05 | Biomadison, Inc. | Methods and compounds for increasing sensitivity of botulinum assays |
| US10908146B2 (en) | 2011-06-01 | 2021-02-02 | Biomadison, Inc. | Compositions and methods for improving sensitivity in cell based assays |
| KR101853103B1 (ko) | 2011-06-01 | 2018-04-27 | 바이오메디슨, 인코퍼레이티드 | 비-ｆｒｅｔ 보툴리눔 검정법 |
| US12422428B2 (en) | 2011-06-01 | 2025-09-23 | Biomadison, Inc. | Compositions and methods for cell-based assays |
| EP2798077A2 (en) | 2011-12-31 | 2014-11-05 | Allergan, Inc. | Highly Sensitive Cell-Based Assay to Detect the Presence of Active Botulinum Neurotoxin Serotype-A |
| WO2013103983A1 (en) * | 2012-01-06 | 2013-07-11 | The Research Foundation Of State University Of New York | High throughput screening of live cells |
| GB201219024D0 (en) * | 2012-10-23 | 2012-12-05 | Syntaxin Ltd | Assay |
| LT3030905T (lt) | 2013-08-09 | 2018-01-25 | Biomadison, Inc. | Padidinto jautrumo botulino toksino tyrimas |
| WO2015061738A1 (en) * | 2013-10-25 | 2015-04-30 | Biomadison, Inc. | Enzyme assay with duplicate fluorophores |
| KR20170026624A (ko) | 2014-07-07 | 2017-03-08 | 알러간, 인코포레이티드 | 조직 샘플에서 절단된 snap25를 검출하는 방법 |
| AU2015301737B2 (en) * | 2014-08-12 | 2021-01-28 | Biomadison, Inc. | Botulinum neurotoxins with modified light chain specifity and methods for producing same |
| CN104359883A (zh) * | 2014-11-13 | 2015-02-18 | 贵阳医学院 | 人源沉默信息调节因子6的活性荧光检测方法 |
| EP3590500B1 (en) | 2014-12-23 | 2025-06-25 | Merz Pharma GmbH & Co. KGaA | Botulinum toxin prefilled container |
| US10676723B2 (en) | 2015-05-11 | 2020-06-09 | David Gordon Bermudes | Chimeric protein toxins for expression by therapeutic bacteria |
| CN105784656B (zh) * | 2016-03-16 | 2019-01-01 | 大连理工大学 | 一种检测活细胞内RhoGDIα蛋白活性的生物探针 |
| TWI737742B (zh) | 2016-06-22 | 2021-09-01 | 德商梅茲製藥有限兩合公司 | 肉毒桿菌毒素的預填充式注射器系統、具有其之套組以及其之使用 |
| KR102480965B1 (ko) | 2016-09-13 | 2022-12-26 | 알레간 인코포레이티드 | 안정화된 비단백질 클로스트리듐 독소 조성물 |
| US11129906B1 (en) | 2016-12-07 | 2021-09-28 | David Gordon Bermudes | Chimeric protein toxins for expression by therapeutic bacteria |
| CN108305912B (zh) * | 2017-01-11 | 2024-03-26 | 中国科学院上海微系统与信息技术研究所 | 具有波长选择性的石墨烯仿生光探测器及其制备方法 |
| WO2018224561A1 (en) * | 2017-06-07 | 2018-12-13 | Original G B.V. | Method for detecting food spoilage microbes |
| US12158470B2 (en) * | 2018-03-09 | 2024-12-03 | Sartorius Bioanalytical Instruments, Inc. | Methods and compositions for live cell analysis of intracellular ATP |
| CN108333342A (zh) * | 2018-05-14 | 2018-07-27 | 广东药科大学 | 一种快速检测牛奶中四环素类药物残留的方法 |
| CA3111674A1 (en) * | 2018-09-28 | 2020-04-02 | Ipsen Biopharm Limited | Cell-based clostridal neurotoxin assays |
| CN109211863B (zh) * | 2018-10-26 | 2021-04-20 | 大连民族大学 | 利用Eu2+f-f跃迁光谱检测爆炸物TNP的方法 |
| TW202113072A (zh) * | 2019-06-07 | 2021-04-01 | 美商辛那皮克研究有限責任公司 | 基因工程化細胞 |
| US12416634B2 (en) * | 2020-06-01 | 2025-09-16 | Galderma Holding SA | SNAP-25 reporter constructs and methods of using the same |
| CN112557353B (zh) * | 2020-12-17 | 2022-02-18 | 天津工业大学 | 一种基于延迟发光光谱的细胞活力检测方法和装置 |
| EP4621057A1 (en) * | 2022-11-18 | 2025-09-24 | Biolinks Inc. | Method for detection or activity measurement of cell-based botulinum toxin by using recombinant fluorescent protein |
| KR20250158928A (ko) * | 2024-04-30 | 2025-11-07 | 주식회사 바이오링크스 | 앵커 단백질을 이용한 미광성 세포기반의 보툴리눔 독소의 검출 및 활성 평가방법 |
Family Cites Families (30)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5804604A (en) * | 1989-12-21 | 1998-09-08 | Biogen, Inc. | Tat-derived transport polypeptides and fusion proteins |
| JPH05505102A (ja) * | 1989-12-21 | 1993-08-05 | ホワイトヘツド・インスチチユート・フオー・バイオメデイカル・リサーチ | 真核細胞の中に分子を配達する方法 |
| US5962637A (en) * | 1994-06-03 | 1999-10-05 | Microbiological Research Authority | Toxin assay |
| GB9411138D0 (en) * | 1994-06-03 | 1994-07-27 | Microbiological Res Authority | Toxin assay |
| GB9508204D0 (en) * | 1995-04-21 | 1995-06-07 | Speywood Lab Ltd | A novel agent able to modify peripheral afferent function |
| US6069010A (en) | 1995-09-11 | 2000-05-30 | Axys Pharmaceuticals, Inc. | High throughput gene inactivation with large scale gene targeting |
| US6803188B1 (en) * | 1996-01-31 | 2004-10-12 | The Regents Of The University Of California | Tandem fluorescent protein constructs |
| US5965699A (en) * | 1996-11-06 | 1999-10-12 | The United States Of America As Represented By The Secretary Of The Army | Assay for the proteolytic activity of serotype a from clostridium botulinum |
| WO1999029721A1 (en) | 1997-12-10 | 1999-06-17 | Washington University | Anti-pathogen system and methods of use thereof |
| EP1073763A4 (en) | 1998-04-28 | 2002-08-07 | Univ Washington | METHODS FOR TRANSDUCING FUSION MOLECULES |
| US7090976B2 (en) * | 1999-11-10 | 2006-08-15 | Rigel Pharmaceuticals, Inc. | Methods and compositions comprising Renilla GFP |
| DE69903337T2 (de) | 1998-10-30 | 2003-08-21 | Cellomics, Inc. | Ein screeningsystem auf zellbasis |
| DE19856897A1 (de) * | 1998-12-10 | 2000-06-15 | Biotecon Ges Fuer Biotechnologische Entwicklung & Consulting Mbh | Therapeutikum zur Unterdrückung von Schnarchgeräuschen |
| JP2002531113A (ja) | 1998-12-10 | 2002-09-24 | ワシントン大学 | 蛋白質の形質導入システムおよびその使用法 |
| US20020076741A1 (en) | 2000-02-11 | 2002-06-20 | Tencza Sarah Burroughs | Peptide biosensors for anthrax protease |
| US6325899B1 (en) * | 2000-03-10 | 2001-12-04 | Action Caps, Llc | Disposable and recyclable intermediates for use in electrostatic coating processes |
| FR2809733B1 (fr) * | 2000-06-02 | 2004-04-30 | Inst Nat Sante Rech Med | Substrat peptidique reconnu par la toxine botulique de type bont/b et son utilisation pour doser et/ou detecter ladite toxine ou des inhibiteurs correspondants |
| DE60131468D1 (de) | 2000-09-25 | 2007-12-27 | U S Medical Res Inst Of Infect | Hoch-durchsatz assays für proteolytische aktivitäten von clostridium neurotoxinen |
| AU2002217839A1 (en) * | 2000-11-22 | 2002-06-03 | The Regents Of The University Of California | A method to measure the activation state of signaling pathways in cells |
| CA2390686A1 (en) * | 2001-06-14 | 2002-12-14 | Pierre Chambon | Cofactor-based screening method for nuclear receptor modulators and related modulators |
| US20080064054A1 (en) * | 2001-08-28 | 2008-03-13 | Ester Fernandez-Salas | Fluorescence resonance energy transfer (fret) assays for clostridial toxin activity |
| US8022172B2 (en) * | 2001-08-28 | 2011-09-20 | Allergan, Inc. | Luminescence resonance energy transfer (LRET) assays for clostridial toxin activity |
| US7208285B2 (en) * | 2001-08-28 | 2007-04-24 | Allergan, Inc. | Fret protease assays for botulinum serotype A/E toxins |
| US7332567B2 (en) * | 2001-08-28 | 2008-02-19 | Allergan, Inc. | Fret protease assays for clostridial toxins |
| US7374896B2 (en) * | 2001-08-28 | 2008-05-20 | Allergan, Inc. | GFP-SNAP25 fluorescence release assay for botulinum neurotoxin protease activity |
| US7183066B2 (en) * | 2002-09-27 | 2007-02-27 | Allergan, Inc. | Cell-based fluorescence resonance energy transfer (FRET) assays for clostridial toxins |
| ES2374822T3 (es) * | 2004-02-24 | 2012-02-22 | Allergan, Inc. | Ensayo de selección de toxinas botul�?nicas. |
| US7399607B2 (en) * | 2004-09-22 | 2008-07-15 | Allergan, Inc. | Fluorescence polarization assays for determining clostridial toxin activity |
| EP2293064B1 (en) * | 2005-04-05 | 2012-04-04 | Allergan, Inc. | Lipophilic dye-based FRET assays for clostridial toxin activity |
| CA2602972C (en) * | 2005-10-12 | 2013-09-24 | Allergan, Inc. | Assays of molecular or subcellular interactivity using depolarization after resonance energy transfer (daret) |
-
2002
- 2002-09-27 US US10/261,161 patent/US7183066B2/en not_active Expired - Lifetime
-
2003
- 2003-09-04 RU RU2005109385/15A patent/RU2005109385A/ru not_active Application Discontinuation
- 2003-09-04 CN CNB038228629A patent/CN1296712C/zh not_active Expired - Lifetime
- 2003-09-04 CA CA2500040A patent/CA2500040C/en not_active Expired - Lifetime
- 2003-09-04 KR KR1020057005144A patent/KR20050071506A/ko not_active Withdrawn
- 2003-09-04 JP JP2004540055A patent/JP4554369B2/ja not_active Expired - Fee Related
- 2003-09-04 BR BR0314795-9A patent/BR0314795A/pt not_active Application Discontinuation
- 2003-09-04 DK DK03774466.1T patent/DK1543329T3/en active
- 2003-09-04 MX MXPA05003128A patent/MXPA05003128A/es unknown
- 2003-09-04 PL PL03376103A patent/PL376103A1/xx not_active Application Discontinuation
- 2003-09-04 AU AU2003282790A patent/AU2003282790B2/en not_active Expired
- 2003-09-04 EP EP03774466.1A patent/EP1543329B1/en not_active Revoked
- 2003-09-04 WO PCT/US2003/028092 patent/WO2004029576A2/en not_active Ceased
-
2005
- 2005-02-21 ZA ZA2005/01500A patent/ZA200501500B/en unknown
- 2005-02-25 NO NO20051049A patent/NO20051049L/no unknown
- 2005-03-13 IL IL167410A patent/IL167410A/en active IP Right Grant
- 2005-03-23 CO CO05026476A patent/CO5721032A2/es not_active Application Discontinuation
-
2007
- 2007-01-09 US US11/621,212 patent/US20070122858A1/en not_active Abandoned
-
2008
- 2008-08-15 US US12/192,925 patent/US7749759B2/en not_active Expired - Fee Related
-
2009
- 2009-10-02 AU AU2009222589A patent/AU2009222589B2/en not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| WO2004029576A2 (en) | 2004-04-08 |
| US7183066B2 (en) | 2007-02-27 |
| IL167410A (en) | 2011-12-29 |
| MXPA05003128A (es) | 2005-06-22 |
| CN1685234A (zh) | 2005-10-19 |
| AU2003282790A1 (en) | 2004-04-19 |
| BR0314795A (pt) | 2005-07-26 |
| US20070122858A1 (en) | 2007-05-31 |
| AU2009222589B2 (en) | 2011-12-22 |
| DK1543329T3 (en) | 2016-12-05 |
| NO20051049D0 (no) | 2005-02-25 |
| NO20051049L (no) | 2005-06-21 |
| ZA200501500B (en) | 2005-10-26 |
| US20090117572A1 (en) | 2009-05-07 |
| EP1543329A2 (en) | 2005-06-22 |
| CO5721032A2 (es) | 2007-01-31 |
| PL376103A1 (en) | 2005-12-12 |
| AU2003282790B2 (en) | 2009-07-02 |
| EP1543329B1 (en) | 2016-08-03 |
| US20040072270A1 (en) | 2004-04-15 |
| US7749759B2 (en) | 2010-07-06 |
| AU2009222589A1 (en) | 2009-10-29 |
| JP4554369B2 (ja) | 2010-09-29 |
| KR20050071506A (ko) | 2005-07-07 |
| WO2004029576A3 (en) | 2004-06-10 |
| JP2006502378A (ja) | 2006-01-19 |
| EP1543329A4 (en) | 2006-10-18 |
| CN1296712C (zh) | 2007-01-24 |
| CA2500040C (en) | 2014-12-23 |
| CA2500040A1 (en) | 2004-04-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| RU2005109385A (ru) | Клеточные анализы резонаннсного переноса энергии флуоресценции (fret) клостридиальных токсинов | |
| JP2006502378A5 (https=) | ||
| EP1901069B1 (en) | Fret protease assays for clostridial toxins | |
| US7208285B2 (en) | Fret protease assays for botulinum serotype A/E toxins | |
| EP1807698B1 (en) | Assay for determining clostridial toxin activity combining fret and fluorescence polarization | |
| KR100487715B1 (ko) | 염료 이합체화에 기초한 형광발생 프로테아제 기질 | |
| JP2004505636A (ja) | 二色蛍光測定によるプロテアーゼ検査 | |
| Tomo et al. | Nearest neighbor analysis of D1 and D2 subunits in the photosystem II reaction center using a bifunctional cross‐linker, hexamethylene diisocyanate | |
| AU2008201932B2 (en) | Fret protease assays for clostridial toxins | |
| Kölbel et al. | Native state dynamics affects the folding transition of porcine pancreatic phospholipase A2 | |
| Morimoto et al. | Key elements for protein foldability revealed by a combinatorial approach among similarly folded but distantly related proteins | |
| Puntambekar | The synthesis, characterization and refolding of Aminotyrosyl derivatives of Ribonuclease A: a spectroscopic probe for monitoring the CIS/TRANS isomerization of Proline-114 | |
| AU2008202928A1 (en) | Fret protease assays for clostridial toxins | |
| Lee et al. | Protein structure, spectral properties, and photobiological function of lumazine protein | |
| HK1138316A (en) | Fluorescence polarization assays for determining clostridial toxin activity |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FA92 | Acknowledgement of application withdrawn (lack of supplementary materials submitted) |
Effective date: 20071015 |