LT3030905T - Padidinto jautrumo botulino toksino tyrimas - Google Patents

Padidinto jautrumo botulino toksino tyrimas

Info

Publication number
LT3030905T
LT3030905T LTEP14761444.0T LT14761444T LT3030905T LT 3030905 T LT3030905 T LT 3030905T LT 14761444 T LT14761444 T LT 14761444T LT 3030905 T LT3030905 T LT 3030905T
Authority
LT
Lithuania
Prior art keywords
botulinum toxin
improved sensitivity
toxin assay
assay
sensitivity
Prior art date
Application number
LTEP14761444.0T
Other languages
English (en)
Inventor
Ward C. Tucker
Tim PIAZZA
Original Assignee
Biomadison, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=52461971&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=LT3030905(T) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Biomadison, Inc. filed Critical Biomadison, Inc.
Publication of LT3030905T publication Critical patent/LT3030905T/lt

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • A—HUMAN NECESSITIES
    • A47—FURNITURE; DOMESTIC ARTICLES OR APPLIANCES; COFFEE MILLS; SPICE MILLS; SUCTION CLEANERS IN GENERAL
    • A47C—CHAIRS; SOFAS; BEDS
    • A47C15/00—Other seating furniture
    • A47C15/004—Seating furniture for specified purposes not covered by main groups A47C1/00 or A47C9/00
    • A47C15/006—Floating seats
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/33—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Clostridium (G)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Optics & Photonics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
LTEP14761444.0T 2013-08-09 2014-08-08 Padidinto jautrumo botulino toksino tyrimas LT3030905T (lt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361864436P 2013-08-09 2013-08-09
PCT/US2014/050424 WO2015021433A1 (en) 2013-08-09 2014-08-08 Botulinum toxin assay with improved sensitivity

Publications (1)

Publication Number Publication Date
LT3030905T true LT3030905T (lt) 2018-01-25

Family

ID=52461971

Family Applications (2)

Application Number Title Priority Date Filing Date
LTEP17183975.6T LT3270162T (lt) 2013-08-09 2014-08-08 Padidinto jautrumo botulino toksino tyrimas
LTEP14761444.0T LT3030905T (lt) 2013-08-09 2014-08-08 Padidinto jautrumo botulino toksino tyrimas

Family Applications Before (1)

Application Number Title Priority Date Filing Date
LTEP17183975.6T LT3270162T (lt) 2013-08-09 2014-08-08 Padidinto jautrumo botulino toksino tyrimas

Country Status (7)

Country Link
US (5) US9526345B2 (lt)
EP (3) EP3030905B9 (lt)
KR (2) KR102246789B1 (lt)
ES (1) ES2818986T5 (lt)
LT (2) LT3270162T (lt)
TW (1) TWI583794B (lt)
WO (1) WO2015021433A1 (lt)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3030905B9 (en) * 2013-08-09 2024-04-03 Biomadison, Inc. Botulinum toxin assay with improved sensitivity
LT3404112T (lt) 2013-10-25 2020-12-28 Biomadison, Inc. Fermento analizė su dublikuotais fluoroforais
AU2015301737B2 (en) * 2014-08-12 2021-01-28 Biomadison, Inc. Botulinum neurotoxins with modified light chain specifity and methods for producing same
US12019066B2 (en) * 2016-05-16 2024-06-25 Biomadison, Inc. Assay with synaptobrevin based moiety
JP6741788B2 (ja) * 2016-05-24 2020-08-19 メディトックス インク． レポーターモイエティ、基質モイエティ及び脱安定化モイエティを含むポリペプチドをコーディングする組み換えポリヌクレオチド、それを含む宿主細胞、並びにその用途
EP3825689A3 (en) 2018-11-29 2021-09-15 Hugel Inc. A cell-based method for determining an activity of botulinum toxin
AR119109A1 (es) * 2019-06-07 2021-11-24 Synaptic Res Llc Células genéticamente manipuladas
US20220326221A1 (en) * 2019-08-13 2022-10-13 Synaptic Research, Llc Cells Highly Sensitive to Clostridial Neurotoxin
WO2023205355A1 (en) * 2022-04-21 2023-10-26 Biomadison, Inc. Cell based assays for botulinum neurotoxin

Family Cites Families (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6699966B1 (en) * 1996-07-08 2004-03-02 University Of Massachusetts Proteins within the type E botulinum neurotoxin complex
US20080064054A1 (en) 2001-08-28 2008-03-13 Ester Fernandez-Salas Fluorescence resonance energy transfer (fret) assays for clostridial toxin activity
US7208285B2 (en) 2001-08-28 2007-04-24 Allergan, Inc. Fret protease assays for botulinum serotype A/E toxins
JP2003079365A (ja) 2001-09-07 2003-03-18 Kanegafuchi Chem Ind Co Ltd 環境有害化学物質の高感度検出法
US7183066B2 (en) 2002-09-27 2007-02-27 Allergan, Inc. Cell-based fluorescence resonance energy transfer (FRET) assays for clostridial toxins
EP1578382B1 (en) 2002-10-31 2009-09-09 Wisconsin Alumni Research Foundation Botulinum neurotoxin b receptors and use thereof
EP2332959B1 (en) * 2003-12-19 2014-11-26 Wisconsin Alumni Research Foundation Method and compositions for detecting botulinum neurotoxin
ATE533853T1 (de) * 2004-02-24 2011-12-15 Allergan Inc Botulinumtoxin-screening-assays
US8497081B2 (en) * 2004-02-24 2013-07-30 Allergan, Inc. Botulinum toxin screening assays
US20080274480A1 (en) * 2004-08-04 2008-11-06 Allergan, Inc. Botulinum Toxin Type a Immunoresistant Assay
US7399607B2 (en) 2004-09-22 2008-07-15 Allergan, Inc. Fluorescence polarization assays for determining clostridial toxin activity
JP2008535486A (ja) * 2005-03-15 2008-09-04 アラーガン、インコーポレイテッド クロストリジウム毒素標的細胞に対する改変された標的能力を有する修飾クロストリジウム毒素
US7449299B2 (en) * 2006-03-10 2008-11-11 David Bauer Quantum dot nanoparticle-based universal neurotoxin biosensor
EP2178905B1 (en) * 2007-07-26 2013-11-06 Allergan, Inc. Methods of activiting clostridial toxins
WO2009035476A1 (en) * 2007-09-14 2009-03-19 Biosentinel, Llc Resonance energy transfer assay with cleavage sequence and spacer
PT2271670E (pt) 2008-03-14 2014-11-28 Allergan Inc Ensaio imunobaseado da atividade do serotipo a da toxina botulínica
WO2010085473A1 (en) 2009-01-20 2010-07-29 Trustees Of Tufts College Methods for the delivery of toxins or enzymatically active portions thereof
US10246492B2 (en) * 2009-10-16 2019-04-02 Biomadison, Inc. Botulinum assay with synaptobrevin substrate moiety
CN102906254A (zh) 2010-03-26 2013-01-30 诺维信公司 热稳定性肌醇六磷酸酶变体
JP2013534415A (ja) * 2010-06-11 2013-09-05 シナプティック リサーチ，リミテッド ライアビリティ カンパニー Ｎ末端ルール・プロテアーゼ同定方法及びその使用法
CN103154257A (zh) * 2010-09-26 2013-06-12 大玉企业有限公司 重组大分子生成方法
LT2715355T (lt) * 2011-06-01 2017-06-12 Biomadison, Inc. Ne fret (fluorescencijos rezonansinės energijos perdavimas) būdu atliekamas botulino tyrimas
US9303285B2 (en) * 2012-01-04 2016-04-05 Biomadison, Inc. Methods and compounds for increasing sensitivity of botulinum assays
US11325954B2 (en) * 2011-06-01 2022-05-10 Biomadison, Inc. Compositions and methods for stability testing of botulinum toxin
US10908146B2 (en) * 2011-06-01 2021-02-02 Biomadison, Inc. Compositions and methods for improving sensitivity in cell based assays
KR101640694B1 (ko) * 2011-09-29 2016-07-18 셀스냅, 엘엘씨 독소생산능 시험용 조성물 및 방법
WO2013102088A2 (en) * 2011-12-31 2013-07-04 Allergan, Inc. Highly Sensitive Cell-Based Assay to Detect the Presence of Active Botulinum Neurotoxin Serotype-A
KR102296109B1 (ko) 2012-01-04 2021-08-30 바이오메디슨, 인코퍼레이티드 보툴리눔 분석 감도를 증진시키기 위한 방법 및 화합물
EP2823053B1 (en) 2012-03-07 2017-08-23 Merz Pharma GmbH & Co. KGaA Means and methods for determining neurotoxin activity based on a modified luciferase
US8778623B2 (en) * 2012-09-14 2014-07-15 Wisconsin Alumni Research Foundation Compositions and methods of using differentiated cells sensitized to botulinum neurotoxin
WO2014060373A1 (en) 2012-10-16 2014-04-24 Merz Pharma Gmbh & Co. Kgaa Cellular test systems for the determination of the biological activities of neurotoxin polypeptides
EP3030905B9 (en) * 2013-08-09 2024-04-03 Biomadison, Inc. Botulinum toxin assay with improved sensitivity
LT3404112T (lt) * 2013-10-25 2020-12-28 Biomadison, Inc. Fermento analizė su dublikuotais fluoroforais
AU2015301737B2 (en) * 2014-08-12 2021-01-28 Biomadison, Inc. Botulinum neurotoxins with modified light chain specifity and methods for producing same
WO2017201079A1 (en) * 2016-05-16 2017-11-23 Biomadison, Inc. Improved assay with synaptobrevin based moiety
WO2020219358A2 (en) * 2019-04-17 2020-10-29 Children's Medical Center Corporation Catalytically inactive botulinum neurotoxin-like toxins and uses thereof

Also Published As

Publication number Publication date
US20200363412A1 (en) 2020-11-19
TW201514307A (zh) 2015-04-16
US20190145974A1 (en) 2019-05-16
ES2818986T5 (es) 2024-01-25
EP3030905B9 (en) 2024-04-03
KR20160042971A (ko) 2016-04-20
EP3030905B1 (en) 2017-10-04
EP3030905B2 (en) 2024-01-10
LT3270162T (lt) 2020-09-10
ES2818986T3 (es) 2021-04-14
TWI583794B (zh) 2017-05-21
EP3030905A1 (en) 2016-06-15
US10191051B2 (en) 2019-01-29
KR20210047982A (ko) 2021-04-30
US20150159193A1 (en) 2015-06-11
KR102246789B1 (ko) 2021-04-29
US20170097350A1 (en) 2017-04-06
KR102349256B1 (ko) 2022-01-07
EP3742165C0 (en) 2023-06-21
EP3270162B2 (en) 2023-08-16
EP3742165B1 (en) 2023-06-21
EP3270162B1 (en) 2020-07-01
WO2015021433A1 (en) 2015-02-12
EP3270162A1 (en) 2018-01-17
US20220244256A1 (en) 2022-08-04
EP3742165A1 (en) 2020-11-25
US11333664B2 (en) 2022-05-17
US10768178B2 (en) 2020-09-08
US9526345B2 (en) 2016-12-27

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